Captive Wild Animals Research Articles

Toxoplasma gondii among captive wild mammals in zoos in Brazil and Cuba: seroprevalence and associated risk factors.

Toxoplasma gondii is a zoonotic parasite of worldwide distribution that can infect several species of homeothermic animals. Few studies have evaluated the exposure of captive wild animals to T. gondii. This study involved a serological survey of anti-T. gondii antibodies in mammals kept in Cuba's National Zoo (PZN) and in the Rio de Janeiro Zoo (RIOZOO) in Brazil. The study consisted of a total of 231 serum samples from mammals, 108 from PZN and 123 from RIOZOO. All the samples were subjected to IgG anti-T. gondii testing by means of the inhibition ELISA method and the modified agglutination test, respectively. T. gondii antibodies were detected in 85.2% samples from PZN and 32.5% samples from RIOZOO. At the PZN, Perissodactyla (92.3%) was the order with the highest serological prevalence rate, whereas at the RIOZOO, the order Primates (46.7%) stood out (p<0.05). In addition to this association, the origin of the PZN animals was also associated with T. gondii infection. This finding demonstrates the need for constant veterinary monitoring of captive wild mammals in order to link the serological diagnosis with clinical alterations indicative of toxoplasmosis.

Occurrence of KPC-Producing Escherichia coli in Psittaciformes Rescued from Trafficking in Paraíba, Brazil.

The emergence and spread of antimicrobial resistance pose a threat to public health globally. Antibiotic-resistant bacteria and genes can disseminate among environments, animals and humans. Therefore, investigation into potential reservoirs of multidrug-resistant bacteria is of great importance to the understanding of putative transmission routes of resistant bacteria and resistance genes. This study aimed to report the occurrence of Escherichia coli harboring the Klebsiella pneumoniae carbapenemase-producing gene (blaKPC) in Psittaciformes rescued from wildlife trafficking in Paraíba State, Brazil. Cloacal swabs were collected from thirty birds and cultured by conventional microbiology using MacConkey and serum tryptone glucose glycerol (STGG) media supplemented with selective antimicrobials. E. coli isolates (n = 43) were identified by phenotypic tests and confirmed by MALDI-TOF. Antimicrobial susceptibility profiles were determined by means of Kirby–Bauer test. All isolates were further screened for extended-spectrum beta-lactamase (ESBL) production, and putative genes encoding ESBL were investigated by PCR. Additionally, blaKPC-harboring strains were genotyped by REP-PCR. A total of 43 E. coli phenotypically resistant isolates were recovered. The highest resistance rate was observed against ciprofloxacin. Among the resistance genes, only blaKPC was found in seven different birds from three species. According to the genotyping, these seven isolates belonged to four different strains. To date, this is the first report on the occurrence of KPC-E. coli in Psittaciformes rescued from trafficking in Northeastern Brazil. Due to the high clinical importance of KPC-E. coli, our findings suggest that wild animals in captivity at wildlife rescue centers can play a role as reservoirs of bacteria that are resistance to Critically Important antimicrobials in human medicine.

Estimating the Cultural Value of Wild Animals in the Qinling Mountains, China: A Choice Experiment.

Simple SummaryThe presence of wildlife makes an important contribution to ecosystem services, and in the hot spots of biodiversity conservation, the results of wild animal value evaluation can provide a basis for the formulation of ecological compensation standards. Applying the choice experiment method, we assessed the value of wild animals in the surrounding areas of the Changqing National Nature Reserve in China from the tourist perspective. The results of our field survey suggest that the cultural value of wild animals, measured in terms of willingness-to-pay, is significantly higher than that of the infrastructure and other natural landscape features. These findings can provide a basis for the rational allocation of resources for wild animal conservation.In this study, we use the choice experiment method to evaluate the value of wild animals in the surrounding areas of the Changqing National Nature Reserve in China. For areas focused on biodiversity conservation, the results of wild animal value evaluation can provide a basis for the formulation of local ecological compensation standards. We identified wild animals, natural landscape features, infrastructure and facilities, and ticket price as major attributes determining the utility and value of the reserve area. The results of our field survey suggest that the cultural value of wild animals is significantly higher than the value of the infrastructure and other natural landscapes. Tourists indicated a willingness-to-pay of 31.4 Yuan to see wild animals in captivity, compared to 71.9 Yuan to see wild animals in the field. Moreover, tourists with different characteristics have different preferences for the cultural value of wildlife. Female tourists have a higher willingness-to-pay than male tourists. In addition, as age, education, and income level increase, tourists’ willingness-to-pay also increases. These conclusions can provide a reference for local government to formulate wildlife protection compensation and ecotourism ticket pricing.

Anachronic Fruit Traits and Natural History Suggest Extinct Megafauna Herbivores as the Dispersers of an Endangered Tree.

Megafaunal seed dispersal syndrome refers to a group of traits attributed to the evolution of plants in the presence of large mammals. Present-day plants that bear these traits in areas where megafauna are absent are presumed to represent anachronic dispersal systems. Gomortega keule is an endangered tree species from a monotypic family (Gomortegaceae), endemic to Chile. Its fruit traits suggest adaptation to seed dispersal by large vertebrates; however, none are present today along its area of distribution. Here, we conducted a detailed revision on the fruit morphology of G. keule to examine whether its fruit traits fit a megafaunal dispersal syndrome. Additionally, we examined the fruit processing behavior of large domestic and captive wild animals fed with G. keule fruits, and its effect on germination. G. keule fruits had traits consistent with those of a Type 1 megafaunal fruit. Compared to intact, whole stones, seed germination probabilities decreased when fruits were handled by animals, suggesting that the seed was damaged during mastication and/or ingestion. Moreover, results from our feeding trials with elephants may also imply low efficiency of extinct gomphotheres as seed dispersers of this species. Our results also suggest that although domestic animals may disperse G. keule, it is unlikely that at present they can substitute the services of its original dispersers. Further investigation on seedling survival, local livestock management and forest management practices may help reinstate sexual regeneration in G. keule. Finally, integrating observations on fruit ecology and local people’s knowledge with experimental data enriches our species-centered approach and may help to address regeneration problems in other endangered plants.

Hypermucoviscous/hypervirulent and extensively drug-resistant QnrB2-, QnrS1-, and CTX-M-3-coproducing Klebsiella pneumoniae ST2121 isolated from an infected elephant (Loxodonta africana)

The rapid dissemination of extended-spectrum β-lactamases (ESBLs)-producing Enterobacterales from different spheres worldwide over recent years has become a serious problem in both human and veterinary medicine. CTX-M-3-type ESBL has only been reported on few occasions, and in Brazil the blaCTX-M-3 gene has been identified only once in clinical strains. In this study, we aimed to molecularly characterize a hypermucoviscous (hm), hypervirulent (hv), and extensively drug-resistant (XDR) Klebsiella pneumoniae strain isolated from a lung tissue culture of an infected elephant. The A246 strain belonged to ST2121 and presented hm phenotype, hypervirulence-associated genes, and carried blaCTX-M-3 and plasmid-mediated quinolone resistance genes (qnrB2 and qnrS1) on an IncFII-IncQ1-IncM1 multireplicon plasmid (pA246-CTX-M-3, ∼ 162 kb). A novel genetic context of blaCTX-M-3, in which a 482-bp ISEcp1 was truncated by an IS26, was also harbored by pA246-CTX-M-3. Furthermore, in vivo experiments revealed that the hm/hv A246 strain killed 100 % of the Galleria mellonella larvae at 72 h post-infection. Our findings evidence the intercontinental dissemination of a rare K. pneumoniae ST2121 and the multidrug resistance IncFII-IncQ1-IncM1 plasmid. Therefore, to the best of our knowledge, this is the first report of an XDR K. pneumoniae coproducing CTX-M-3, QnrB2, and QnrS1 isolated from captive wild animals.

Formulating fungal spores to prevent infection by trichostrongylids in a zoological park: Practical approaches to a persisting problem

Two different formulations containing a blend of spores of the filamentous fungi Mucor circinelloides and Duddingtonia flagrans have been assayed against trichostrongylids in wapitis captive in a grassy parcel from a zoological park and dewormed with fenbendazole. One approach (sprayed-on pellets) consisted of providing them, during 10 months, nutritional pellets sprayed the spores just before to be ingested, and the other (formulated pellets) involved the administration of pellets industrially manufactured with the spores for an identical period. Data collected on wapitis treated with fenbendazole without receiving spores during a 10-months interval were considered as controls. The effect of these strategies was evaluated through the analysis of feces collected directly from the soil, by estimating the reduction of the counts of eggs of trichostrongylids per gram of feces (EPG). The possibility of side effects in the digestive tract, respiratory apparatus or on the skin was assessed. The coprological analyses showed that, despite the successfulness of fenbendazole, the values of trichostrongylids egg-output increased four months later above 300 EPG. Through the administration of pellets sprayed spores, a significant EPG reduction of 69% (30–100) was recorded, and 71% (36–100) by giving the wapitis pellets industrially enriched with the spores. No unfavorable effects were observed among the wapitis taking the spores. It is concluded that ingestion of a blend of spores of M. circinelloides and D. flagrans every two days affords a beneficial long-term effect on controlling the infection by trichostrongylids in wild captive animals.

Characteristics and Epidemiology of Extended-Spectrum β-Lactamase-Producing Multidrug-Resistant Klebsiella pneumoniae From Red Kangaroo, China

Due to its drug resistant nature, β-lactamase represents a serious challenge for public health. Extended-spectrum β-lactamase (ESBL) producing Klebsiella pneumoniae clones are increasingly reported worldwide. Little is known about the prevalence and biological characteristics of drug-resistant strains in zoos. During routine surveillance at the Zhengzhou Zoo of China, we found Klebsiella pneumoniae isolate in healthy Red Kangaroos (Macropus Rufus) with severe MDR. The Klebsiella pneumoniae were especially resistant to Cefuroxime Sodium (MIC, > 64 μg/mL), Ceftriaxone (MIC, >8 μg/mL) and Cefepime (MIC, >64 μg/mL), and belonged to ST290. Subsequently, whole genome sequencing (WGS) showed that the Chrome Chr-M297-1 harbored blaDHA–3, blaSHV–1, blaCTX–M–14, fosA5, dfrA3, sul3, etc., and pM297-1.1 [222,864 bp, IncFIB(K)], which carried nine antimicrobial genes including blaCTX–M–14, blaTEM–191, aph(3″)-Ib, aph(6)-Id and qnrS1, etc., and pM297-1.2 [225,763 bp, IncFII(K)] carried 22 antimicrobial genes including blaTEM–1, blaCTX–M–3, aph(3′)-Ia, aac(3)-IIa, aac(6′)-Ib-cr, aadA16, qnrB2, qnrS1, qacEΔ1, mphA, sul1, and dfrA27, etc. A traceability analysis then revealed that these two plasmids were highly similar to those recovered from human clinical samples in some southern cities in Sichuan Province, China (>99%), suggesting that these plasmids are spreading in China. Furthermore, two plasmids harboring conjugal transfer genes facilitated the transmission of antimicrobial genes by conjugation with E. coli J53. Our research shows that the transmission and adaptation of Klebsiella pneumoniae producing ESBLs is occurring in zoo environments, suggesting that zoos may be becoming important potential reservoirs for clinically important drug-resistant genes. It is therefore necessary to monitor the emergence and spread of drug-resistant gene strains in captive wild animals held in zoo environments.

Hunting strategies used in protected areas in the atlantic rainforest of northeastern Brazil

The significance of faunal resources and their utilization potentials for the benefit of mankind is well known, and constitute important natural resources in local communities in the Atlantic forests of region Northeastern Brazil, including in their protected areas. In order to gain access to these resources, hunters have developed a series of techniques and strategies that are described in the present work from interviews with 109 hunters in 4 official conservation units. Fifteen different techniques for the hunting and capture of wild animals have been registered, divided into 3 categories: Trapping (n=27,9%), active search (n=31%) and passive waiting (n=41,1%). All the techniques listed under the active search and passive waiting categories are complemented by the use of firearms. The environmental and administrative characteristics of the studied areas and the socioeconomic profile of the hunters interviewed, influenced the differences in the techniques and hunting strategies. Additional studies concerning these hunting activities will be useful to contribute to proposals for oversight and management plans for hunting in the region, with the objective of attaining sustainable use of faunal resources to the local human communities.

Prevalence and diversity of Gastro-intestinal parasites in captive wild animals in Northern Nigeria.

Copro-parasitological survey of 217 captive wild animals belonging to 17 orders from northern Nigeria was conducted.Faecal samples were collected from the wild animals in zoological gardens in four states and analysed using the flotation andsimple sedimentation techniques. Overall, parasite eggs or cysts were detected in 47% (n=102) of the animals examined.High parasite prevalence, 80%, 75%, 71%, 60 % and 50% were recorded for members of the orders Testudines,Perissodactyla, Artiodactyla, Primates and Rodentia respectively. Multiple infections involving two or three parasite taxawere recorded in some of the captive wild animals examined with a significant association (p&lt;0.05) between the animals andnematode eggs, Taenia eggs and Eimeria oocysts. Others helminth species such as Ascaris, Hookworm, Enterobius,Entamoeba, Trichuris and Strongyloides were also detected. This calls for caution as the wild animals may serve as source ofinfection to attendants and visitors to the zoos.&#x0D; Keywords: parasites, zoonoses, ecology, conservation, wildlife, Nigeria

Making a Tiger's Day: Free-Operant Assessment and Environmental Enrichment to Improve the Daily Lives of Captive Bengal Tigers (Panthera tigris tigris).

There are more captive tigers in the United States than there are wild tigers in the entire world. Many animals under human care engage in problem behaviors such as excessive grooming and aggression, although the origin of these behaviors is typically unknown. Environmental enrichment may mitigate these issues in captive animals of all kinds. In order to individualize enrichment experiences, the current study used a free-operant assessment procedure to establish a menu of most preferred play items and scents among 7 Bengal tigers (Panthera tigris tigris) housed at a sanctuary in southwest Missouri. Each tiger was tested 3 times with scents (cinnamon and Calvin Klein Obsession perfume) and play items (boxes, balls, leaves, and pumpkins). The importance of rigorous assessment of presumed reinforcers among captive wild animals, as well as the difficulty of effectively assessing tigers while ensuring the safety of both the participants and researchers, is discussed.

Granulomatous pneumonia due to pythiosis in captive South American coatis (Nasua nasua)

ABSTRACT: Pythiosis is an emerging infectious disease affecting captive and free-ranging wild animals. We report granulomatous pneumonia due to Pythium insidiosum in two South American coatis (Nasua nasua), who were found dead without any clinical records. Severe granulomatous pneumonia associated with pleural effusion was revealed in the necropsy. Microscopically, variably sized granulomas and pyogranulomas presented negative hyphae profiles at the periphery of their necrotic cores. Grocott methenamine silver stain highlighted these structures, and immunostain (anti- P. insidiosum) was strongly positive. Molecular analysis by polymerase chain reaction amplified P. insidiosum specific DNA. These findings characterized P. insidiosum as a cause of granulomatous pneumonia in coatis and proved that pythiosis needs to be considered in the differential diagnosis of respiratory diseases affecting this species in endemic areas.

Reflections on Potential Risk Factors of Callitrichidae Run Over in an Urban Area: A Case Report of Marmoset Deaths

ABSTRACT Interest in the well being of wild animals in their natural habitats is still lower than that of domesticated animals and captive wild animals. Urban development is one of the events that has the greatest impact on fauna, as it affects the survival and well being of wild species in many ways. This study aimed to record death by being run over of two Callithrix penicillata (black-tufted) marmosets in a fragmented environment by urbanization and to discuss how anthropic intervention modifies species behavior and influences their well being, and to suggest measures which can reduce the occurrence of incidents, thereby contributing to maintaining the fauna and their well being.

CONSERVATION CHALLENGES: THE LIMITATIONS OF ANTEMORTEM TUBERCULOSIS TESTING IN CAPTIVE ASIATIC LIONS (PANTHERA LEO PERSICA).

Genetic diversity of captive wild animals can be enhanced by moving those individuals with valuable genes between collections and through introduction of a new pair from a range country. This requires movement of animals, which is inherent with disease risks, such as the introduction of pathogenic Mycobacterium sp. (MTBC) into a zoological collection. Decisions need to be made based on the outcome of perimovement disease screening using an array of tests, the majority of which are unvalidated in the species. A pair of endangered Asiatic lions (Panthera leo persica) imported from India to the United Kingdom were screened for MTBC using the comparative intradermal tuberculosis (TB) test, the feline interferon-γ blood test, and the experimental bacteriophage assay. Reactions on all three tests prompted screening of the three resident Asiatic lions using the same tests, all of which were negative for MTBC. Based on these test results, the decision had to be made to exclude the genetically valuable pair from the current collection. MTBC could not be identified using further tests, including culture and PCR on a bronchoalveolar lavage, on feces, or on postmortem tissues. This case series highlights the usefulness of a control group when interpreting unvalidated test results for detection of MTBC, the value of training big cats for conscious blood sampling, and the practical implications of placing the comparative intradermal TB test in the eyelids, when dealing with a species that requires a general anesthetic for most hands-on interventions.

The effect of varying laboratory conditions on the locomotor activity of the nocturnal Namaqua rock mouse (Micaelamys namaquensis) and the diurnal Four-striped grass mouse (Rhabdomys dilectus)

Rodents are the most common laboratory animals all over the world, however, most studies on the effects of laboratory conditions on the behavior and physiology of the study animals have been performed on traditional laboratory animals. We investigated the effects of environmental enrichment, lighting conditions and ambient temperature cycles on the locomotor activity of wild trapped, nocturnal Namaqua rock mice and diurnal Four-striped grass mice. When considering the general activity of the two species, the diurnal species showed more variability in locomotor activity than the nocturnal species. Cage enrichment differentially affected the intensity of the locomotor activity in the two species. Despite a decrease in activity, the diurnal species showed more structured rhythms in an enriched cage. Twilight conditions changed the behavior of both species, the active time of the nocturnal animal was contracted to the completely dark hours of the light cycle, while the active time of the diurnal species was extended in the longer daylight hours. The natural light appears to stabilize the entrainment of the diurnal species. The natural ambient temperature cycle caused changes in intensity of activity, but reinforced entrainment in both species. These results show that changes in laboratory housing conditions can affect the activity of captive wild animals and that these effects are species specific. By increasing our understanding of the effects of different environmental factors on the outcomes of experiments, both the results obtained, and the welfare of the captive animals may be improved.

The Design of Musical Instruments for Grey Parrots: An Artistic Contribution toward Auditory Enrichment in the Context of ACI

One particular approach in the context of Animal Computer Interaction (ACI) is auditory enrichment for captive wild animals. Here we describe our research and the methodology used to design musical instruments and interfaces aimed at providing auditory enrichment for grey parrots living in captivity. The paper is divided into three main phases: a project review and classification, sonic experiments at the parrot shelter and the design of musical instruments. The overview of recent projects that involve animals in the interaction and music-generation process highlights the costs and benefits of projects of this kind and provides insights into current technologies in this field and the musical talents of animals. Furthermore, we document a series of sonic experiments conducted at a parrot shelter to develop acoustically enriched environments through the use of musical instruments. These investigations were intended to provide a better understanding of how grey parrots communicate through sound, perceive and respond to auditory stimuli and possibly generate sound and music through the usage of technological devices. Based on the cognitive, physiological, and auditory abilities of grey parrots, and their intrinsic interest in sonic and physical interactions, we finally developed and tested various interactive instrument prototypes and here we present our design results for auditory enrichment in the context of ACI and artistic research.

Study on Gastrointestinal Parasitism of Wild Animals in Captivity at the Zoological Garden of Haramaya University, Ethiopia

Captivity of wild animals and restriction of their movement can lead to stress, resulting in suppressed immune response and reduced disease resistance ability. Wild animals in zoological gardens can predominately affected by various gastro-intestinal parasites. This study was therefore, conducted to determine occurrence, identify types and assess seasonal variation of gastrointestinal parasites in captive animals at the Zoological garden of Haramaya University. The study involved a retrospective investigation of zoo records and fecal examinations. Twenty two fecal samples were collected in December, 2013 from all the animals in the garden and examined using concentration and fecal culture techniques. Sampling was repeated after four months in April, 2014 to assess seasonal variations of the parasites. The result from the retrospective study showed death of 21 animals in the last five years. Fecal examination confirmed presence of gastrointestinal parasites with an overall mean prevalence rate of 73.8%. A total of 9 parasite taxa were identified of which 6 (66.67%) and 4 (33.33%) were helminths and protozoa respectively. No Cestode and Trematode species were recovered. Trichuris spp., Toxocara spp., Strongyloides spp., Toxascaris leonine, Passalurus ambiguous and Ascaridia spp. were the helminth parasites observed in the garden. Entamoeba spp., Isospora spp. and Eimeria spp. were the protozoan parasites encountered. No remarkable seasonal variation in parasitic infection was noticed in the garden. The study revealed occurrence of gastrointestinal parasites in the zoological garden, most of which are zoonotic and hence future studies are needed to determine risks of cross-transmission.

Bandicoot bunkers: training wild-caught northern brown bandicoots (Isoodon macrourus) to use microchip-automated safe refuge

Abstract ContextSoft-release involving supplementary feeding or shelter is commonly used in wildlife reintroduction and rehabilitation projects. However, competition for nestboxes and supplementary feed, as well as predation at feed stations or nestboxes, can reduce the benefits of soft-release. The use of microchip-automated technology can potentially alleviate these concerns, by providing targeted supplementation to only the intended, microchipped animals. AimsWe aimed to train wild-caught northern brown bandicoots, Isoodon macrourus, to use microchip-automated doors to access safe refuge. MethodsBandicoots were trapped from the wild and brought to the Hidden Vale Wildlife Centre, where eight were trained to use the doors in a six-stage process, and then six were trained in a three-stage process, using a peanut butter reward. Key resultsBandicoots learned to use the doors in as few as 3 days. The duration of visits to the door generally increased during training, although the number of visits decreased. ConclusionsThe bandicoots successfully learned to use the microchip-automated doors, which shows that this technology has great potential with wildlife, particularly given the short training times required. ImplicationsThe use of these microchip-automated doors with wildlife has many potential applications, including supplementary feeding stations, nestboxes, monitoring in the wild, as well as enrichment for wild animals in captivity.

High Mortality and Altered Diurnal Activity Pattern of Captive Blackbuck (Antilope cervicapra) in Mrigasthali Enclosure, Pashupatinath Area, Kathmandu

One of the major objectives of keeping the wild animals in captivity is their successful breeding, population growth and future translocation. However, many species have lesser behavioral flexibility and fail to establish a viable population in captive conditions due to poor management, intolerant climatic conditions, competitions with other co-housed species, diseases, etc. Blackbuck (Antilope cervicapra) is a nationally endangered and protected mammalian species of Nepal. Blackbucks have been kept under captivity of Mrigasthali enclosure at Pashupatinath Temple area in Kathmandu since 2004 AD and the population has dwindled sharply in recent years. This study was designed to assess the population trend and diurnal activity pattern of the species in the Mrigasthali enclosure. Population census data for the last fifteen years were analyzed and behavioral samplings were done by ‘Focal animal sampling’ and ‘Scan sampling’ methods from 5th April to 29th July 2016. The study revealed a sharp decrease of the population since the outbreak of the foot-and-mouth disease in 2014 exposing the remnant population into the risk of extirpation. The surviving individuals have the diurnal activity pattern and the time budgets prominently different than that of the wild populations, especially, they invest lesser time on feeding and more time on resting. Living in open areas despite cooler climate, intense competition for food and space with spotted deer and monkeys, lower behavioral flexibility of the species, anthropogenic disturbances, stochasticity related to the smaller population size, etc. were perceived as the major threats to the Blackbuck in Mrigasthali enclosure.

Normalizing Human-Animal Power Relations Through Media: Zoo discourses in Turkey

This study examines zoo discourses on media as a convenient site for probing into human-animal power relations. A form of critical discourse analysis is carried out in national daily news discourse focusing on how zoo discourses portray animals through lexical choices, grammatical structures, and discursive strategies of capitalism, hospitality, and conservation. These strategies overall operate to conceal the domination, oppression, and suffering of captive wild animals behind the benevolent image of the zoo institution promoting conservation, education, and recreation. Through language, animals are constructed, on a superficial level, as subjects who enjoy their lives on natural habitats with their families. Yet further analysis reveals a power abuse in which animals are objectified and commodified for an exclusively human agenda. The study concludes that through the naturalizing effect of discourses human dominance over wild animals are never questioned and the zoos grant animals an instrumental value rather than inherent value.

Different enriched environments for Eurasian lynx in the Barnaul Zoo

Our primary interest is in welfare of Eurasian lynx (Lynx lynx) in the Barnaul Zoo. As discussed below, behavioural researches can reveal much about the welfare of captive wild animals. Eurasian lynx behaviour is studied and time budget is calculated for male and female. The Schorygin’ similarity coefficient of behaviour is determined (87.2%). The male lynx demonstrated low degree of stereotypic behaviour–5.9%, the female lynx–3.8%. We analyzed the differences in their behaviour before and after environmental enrichment. Olfactory environmental enrichment exhibits a decrease of male stereotypic behaviour on 24.3% and of female on 18.8%. Feeding environmental enrichment reduces male stereotypic behaviour on 15.9%, female on 20.3% (with a rat trunk); the male lynx pacing is diminished on 0.9% and female stereotypic behaviour on 1.4% in experiments with meat under fresh-cut grass. Based on our findings, we recommend that lynxes should be housed by mating pair (male with female), with appropriate hiding locations and with the varied feeding, change decorations for captive lynx, carry out tests by olfactory environmental enrichment (with pony droppings and with valerian) and feeding environmental enrichment. It could be concluded that animal welfare depends on keepers’ goodwill relation with captive lynx.