Objective: To analyze the compliance of nursing practice in transfusion therapy, according to current legislation, and their participation in hemovigilance actions. Methodology: descriptive exploratory research held in a teaching hospital in western Parana involving 24 nurses who developed actions hemotherapy. Was used a data collection instrument to assess nursing hemotherapy practice in accordance with the appropriate standards. Results: Of the participants, 58% they refer to be insufficiently informed about it and 63% did not guide the patient about the risks of therapy. A total of 63% label the sample at the nursing station away from the patient, it tends to induction of error considering the demand for labor and not always adequate supply of human resources. And 46% received no training on the subject. Conclusion: The development of training programs for nurses on transfusion therapy may be strategies for qualifying assistance and meet the legislation.