Western Medicine Control Group Research Articles

Effect of Tanyu-Tongzhi recipe on Rho/Rho kinase signal transduction pathway of myocardial ischemia-reperfusion injury in rats

Objective To investigate the influence of Tanyu-Tongzhi (TYTZ)recipe on Rho/Rho kinase signal transduction pathway of myocardial ischemia-reperfusion injury in high fat-fed rats.Methods 60 SD rats were divided into 5 groups randomly,a sham-operated group,a model group,a western medicine control group,a high-dose group and a low-dose group of TYTZ recipe.The model of ischemia reperfusion of the myocardium was reproduced by ligation of left descending artery for 30 min followed by releasing the ligation for 2 hours in rats.The downstream substrates of ROCK myosin light chain (MLC) phosphorylation expression of myocardial tissues in rats were detected by Western blotting method.The levels of RhoA,ROCK Ⅱ mRNA were determined with RT-PCR.Results Compared with the model group,the expression of p-MLC and RhoA,ROCK Ⅱ mRNA were lower in low-dose group of TYTZ,there were significant differences between the two groups(P=0.004、0.003、0.018、0.004,P＜0.01).Conclusion The TYTZ Recipe can protect myocardium from MI/RI.The mechanism of action was related to its inhibiting the protein expression of RhoA,ROCK Ⅱ and p-MLC,restraining the activation of Rho/Rho kinase signal transduction pathway. Key words: Tanyu-Tongzhi recipe; Myocardial ischemia-reperfusion injury; Signal transduction pathway

Systematic evaluation of kidney-tonifying and blood-activating traditional Chinese medicines in treatment of ovulatory disorder infertility

To evaluate clinical studies on effect of traditional Chinese medicines in treating ovulatory disorder infertility by using evidence-based medical method. According to the standard of Cochrane Handbook, the selection standard of the randomized controlled clinical trail on kidney-tonifying and blood-activating traditional Chinese medicines in the treatment of ovulatory disorder infertility was formulated. Literatures in line with the standard were searched through computers among Chinese and English databases. The literatures included were evaluated by evidence-based medicine method, and analyzed with Revman 5. 1. There were 19 articles in line with the standard. Data for pregnancy rate, ovulation rate and miscarriage rate was extracted from them, and heterogeneity test was conducted with Meta, which showed no statistical heterogeneity. Results showed pregnancy rate (n = 17) , RRp 1. 66, 95% C1 [1. 45, 1.92], Z7.06 (P<0.000 01); ovulation rate (n =11) , RRo 1.05, 95% CI[0.97,1. 12], Z 1.25 (P=0.21); miscarriage rate (n =5) , RRM, 0. 24, 95% CI[0. 11,0. 56] , Z 3.33 (P =0. 000 9). After analysis and comparison, it is found that the Chinese medical treatment group was superior to the Western medicine control group in terms of pregnancy rate, which showed statistic significance. Both groups showed no obvious difference in the ovulation rate, and no statistic significance; the miscarriage rate of the Chinese medicine group is obviously lower than that of the Western medicine control group, which indicated statistic significances. This study preliminarily proves the good efficacy and unique advantages of kidney-tonifying and blood-activating traditional Chinese medicines in treating ovulatory disorder infertility.

Clinical observation on the effect of acupuncture treatment to improve digestive system symptoms of malignant tumor patients

Objective To observe the therapeutic effect of acupuncture for treatment of malignant tumor patients with symptoms of digestive system.Methods We carried out a cohort study and enrolled 92 malignant tumor patients hospitalized at Yantai cancer hospital from July 2008 to Jun 2009.They were randomly divided into three groups by the random number table with the ratio of 1 ∶ 1 ∶ 1.The three groups were pattern differentiation acupuncture group that including excess and deficiency syndromes,non pattern differentiation acupuncture group and the western medicine control group.Patients in pattern differentiation acupuncture group accept different acupuncture treatment with different acupoints according to their pattern.Patients in non pattern differentiation acupuncture group only accept acupuncture treatment with fixed acupoints.All patients in the two groups accepted acupuncture treatment one times per day,seven days is a course of treatment.Western medicine control group get routine chemotherapy including CTX,ADM,VCR,VP-16,PDD,MTX 5-FU,and symptomatic and support treatment were added according to the patients symptoms.Efficacy of all groups were evaluated and compared after one course of treatment.Results (1) Comparison of symptom change scores:①Inner group comparison:Patients' symptoms were significantly improved after treatment in pattern differentiation acupuncture group and non pattern differentiation acupuncture group (P＜0.05); The difference of patients' symptoms before and after treatment didn't show statistical significance in western medicine control group differences (P＜0.05).② Comparison among groups:Compared with western medicine control group,the difference among pattern differentiation acupuncture group and non pattern differentiation acupuncture group were statistically significant after treatment (t=2.212,1.989,P＜0.05),the difference between pattern differentiation acupuncture group and non pattern differentiation acupuncture group didn't show statistically significant (t=1.548,P＞0.05).(2) Improvement of digestive system symptoms:Compared with western medicine control group,the difference among pattern differentiation acupuncture group and non pattern differentiation acupuncture group was statistically significant after treatment (x2=15.28,13.45,P＜0.05),the difference between pattern differentiation acupuncture group and non pattern differentiation acupuncture group didn't show statistically significant (x2=9.81,P＞0.05).Conclusion Acupuncture treatment can improve the symptoms of digestive system of malignant tumor patients. Key words: Malignant tumor; Symptoms of digestive system; Acupuncture treatment

Effects of ruanmaijiangzhi mixture on circulating endothelial cells after artery bypass graft in rabbits

Objective To observe the effect of Ruanmaijiangzhi mixture on circulating endothelial cells (CECs) of rabbits by artery bypass graft,to explore the significance of changes in CECs in the process of restenosis after artery bypass graft,and the possibility of drugs to improve the endothelial injury.Methods A New Zealand white rabbit model of right common carotid artery bypass graft was constructed.Thereafter,to randomly divided into four groups:model group,control group,middle dose Ruanmaijiangzhi group,high dose Ruanmaijiangzhi group.Eight weeks after dosing,animals blood CECs level were detected by flow cytometry.Results Whether single-labeled CD146 (0.38 ± 0.15,0.12 ± 0.06,0.08 ±0.02) or two markers count CECs (0.14 ±0.11,0.07 ±0.02,0.03 ±0.01),the model group average percentage of CECs are significantly higher than treatment group (P ＜ 0.05).Chinese medicine group has statistically significant for western medicine control group (single-labeled 0.24 ± 0.11,two markers 0.08 ± 0.05) (P ＜ 0.05).Two groups of Ruanmaijiangzhi showed a positive dose-related correlation.Conclusion Chinese medicine Ruanmaijiangzhi mixture could promote the repair of vascular endothelial injury after artery bypass graft,thereby is helpful to improve restenosis after artery bypass graft. Key words: Ruanmaijiangzhi mixture; Artery bypass graft; Circulating endothelial cells

Study on clinical intervention of Jiawei Shengdan Louxie Sini Fang on coronary heart disease with depression

To study the differentiation of symptoms and signs of major syndrome factors of traditional Chinese medicine and the efficacy of Jiawei Shengdan Louxie Sini Fang combined with methods for nourishing qi and yin, promoting blood circulation, nourishing heart and kidney, soothing and nourishing liver and reducing phlegm. In the randomized controlled clinical trial, patients of coronary heart disease with stable angina and depression are selected and divided into the traditional Chinese medicine intervention group and the western medicine control group. Maintaining the basic treatment with western medicines for coronary heart disease, the Chinese traditional medicine group was administered with Jiawei Shengdan Louxie Sini Fang, while the western medicine control group fluoxetine. Compared with the control group, the traditional Chinese medicine group was better in angina curative effect, syndrome curative effect and HAMA rating scale. In the comparison for sign and symptom score, the test group was better than the control group in short of breath, lack of power, self-sweat, night sweats, insomnia, frequent micturition in night, constipation, abdominal distension, frequent deep sigh, pharynx obstruction and dark color of lips. Intervention with traditional Chinese medicine can ameliorate angina and other symptoms of whole body. It can also improve anxiety scores of patients in order to reduce the impact of depression and anxiety on coronary heart disease.

Effect of Wenyang-Yixin recipe on NF-κB signal transduction pathway of myocardial ischemia-reperfusion injury in rats

Objective To investigate the influence of Wenyang-Yixin recipe(WYYXR) on NF-κB signal transduction pathway of myocardial ischemia-reperfusion injury in rats. Methods 32 SD rats were divided into 4 groups randomly, sham-operated group, model group, western medicine control group and WYYXR group. Sham-operated group and model group were given physiological saline solution (10 ml/kg),estern medicine control group were treated with fluvastatin (4 mg/kg), and self-made Chinese herb was perfusedinto rats in WYYXR group (1 ml/100 g) each time, once a day, for successive three weeks before model making.The model of ischemia reperfusion of the myocardium was reproduced by ligation of left descending artery for 30 min followed by releasing the ligation for 2 hours in rats. NIK, IKKβ and IκBα protein expression of myocardial tissues in rats were detected by western blotting method. And the levels of NF-κBp65mRNA were determined with RT-PCR. Results Compared with model group, the expressions of NIK, IKKβ and NF-κBp65mRNA were lower, and the protein expression of IκBα was increased in WYYXR group, there were significant differences between the two groups (P=0.004> 0.003, 0.018, 0.004,P<0.05) . Conclusion The Wenyang Yixin Recipe can protect myocardium from MI/RI. Key words: Wenyang-Yixin recipe; Myocardial ischemia-reperfusion injury; Signal transduction pathway

Effect of compound Polygonum multiflorum extract on Alzheimer's disease

To observe the clinical effect of compound Polygonum multiflorum extract on Alzheimer's disease (AD). We collected 209 AD patients, among whom 120 were treated with compound Polygonum multiflorum extract as a treatment group, 60 were treated with Polygonum multiflorum extract as a Chinese herb control group, and 29 were treated with Naofukang as a western medicine control group. The scores for the Mini-Mental State Examination (MMSE), Ability of Daily Living Scale (ADL) and the therapeutic effect were assessed and compared before and after the 12 week treatment. After the treatment the scores for MMSE and ADL were improved in all groups. The scores of MMSE and ADL in the compound Polygonum multiflorum extract treatment group were significantly improved compared with the Chinese herb control group and the western medicine control group (P < 0.01). The total effective rate of 93.33% in the compound Polygonum multiflorum extract treatment group was better than 73.33% in the Chinese herb control group and 68.97% in the western medicine control group (P < 0.01). Compound Polygonum multiflorum extract has effect on AD, and it is superior to Polygonum multiflorum extractor Naofukang.

Clinical observation on treatment of endometriosis with acupuncture plus herbs

Objective: To compare the clinical efficacy of combined acupuncture and herbs with danazol in treating endometriosis. Methods: Seventy-eight patients were randomly divided into a combined acupuncture and herbs group (n=40) and a Western medicine control group (n=38). Observation was made on changes in clinical symptoms, signs, serum CA125, β-EP, PGE2 and PGF2α, and adverse reaction to the medicine in the two groups. Results: The overall efficacy was similar in the two groups, but the effects on lumbosacral pain, anal downbearing distention, irregular menstruation and infertility were significantly better in the treatment group than in the control group (P<0.05), and in the treatment group serum CA125, PGE2 and PGF2α, were significantly lower and serum β-EP was significantly higher after treatment than before (P<0.05). Conclusion: Combined use of acupuncture and herbs has a marked effect on endometriosis and the adverse reaction is lower than the control group.

Clinical study on preventing and treating left ventricle remodeling after acute myocardial infarction with integrative Chinese and western medicine

Objective: To explore the clinical efficacy of Xuefu Zhuyu, XFZY) oral liquid combined with western medicine (WM) in reversing left ventricle remodeling (LVR) after acute myocardial infarction (AMI) in patients and its therapeutic mechanism.Methods: Sixty patients conforming to criteria were divided into the treated group and WM control group with randomly stratified method. Thirty cases were assigned to each group, they were given XFZY + WM + general treatment and WM + general treatment respectively. Color Doppler ultrasonography was used to study the changes of LVR before and after treatment. Results: In both groups, before and after treatment theP value of such parameters as the changes of their left diastolic volume (LDV), left systolic volume (LSV), left ventricle ejection fraction (EF) was <0.05, suggesting that the difference of various parameters was significantly different; but when compared with the results of the 12th week after medications, the change of LDV, LSV, EF showed that the LDV, LSV of the treated group were lower than that of the control group, the EF of the treated group was higher than that of the control group, and theP value of various groups was all <0.05, suggesting that the different data of various groups showed significant difference.Conclusion: Conventional WM treatment and XFZY combined with WM could both, in treating AMI, effectively reverse the patients’ LVR; yet the use of integrative Chinese and western medicine on reversing patient’s LVR showed even better results than that of simple WM treatment.

Study on clinical therapeutic effect of composite salvia injection matched with western medicine in treating diabetic foot

Objective: To investigate the clinical effects of composite salvia injection (CSI) matched with western medicine (WM) in treating diabetic foot. Methods: Seventy-two patients, whose diagnosis were confirmed to be diabetic foot, were randomly divided and equally assigned to the comprehensive CSI plus WM treated group and the WM control group, they were treated with the combined CSI and WM and the conventional WM respectively. The therapeutic effect was evaluated in combining with data of electromyogram and hemorrheologic investigation. Results: All the indexes were improved to various extent after treatment in both groups, but the improvement was more significant in the CSI g roup than in the WM group, shown as quickening of motor and sensory nerve conduction velocity of median nerve and lowering of blood viscosity, as compared with those before treatment, P0 01. Conclusion: CSI matched with WM in treating diabetic foot is beneficial for resulting in better effect.