Probe Volume Research Articles

Comments on "Investigation of Histology Region Dielectric Measurements of Heterogeneous Tissues".

In the paper, "Investigation of histology region in dielectric measurements of heterogeneous tissues," by Porter and O'Halloran, the authors utilize a flexible phantom in a layered material dielectric property analysis to quantify the effective sensing volume of a coaxial dielectric probe. Ostensibly, this test has been used by others to characterize the region for which percent variation in the material composition in front of the probe corresponds to percent variation in the computed effective dielectric properties. By employing a compressible material, the authors fail to isolate features that are attributable solely to the probe, itself, and inadvertently incorporate confounding characteristics associated with the compressible nature of the material. The net effect is to exaggerate the probe's sensing volume which undermines conclusions drawn from the subsequent tissue dielectric property studies.

Non-invasive time domain reflectometry probe for transient measurement of water retention curves in structured soils

Transient evaporation method is promising for rapid measurement of soil water retention curve (WRC). Existing apparatuses of transient evaporation method are not ideal for measuring the WRC of structured soils because of the sample disturbance induced by invasive water content probes used. In this study, non-invasive TDR probes with different waveguide layouts (i.e., different number of conductors and waveguide length) were developed to measure the WRC of loess during transient evaporation, while the soil suction is recorded through a tensiometer. Results show that the WRCs of undisturbed and remolded loess measured by a three-conductor non-invasive TDR probe are in good agreement with the results of pressure plate tests. The differences in the air entry value (AEV) and desorption rate between the WRCs measured by the transient evaporation method and the pressure plate measurement are less than 10 %. On the other hand, the WRCs measured by a two-conductor non-invasive TDR probe underestimate the water content for a given suction. The AEV of undisturbed and remolded loess determined by the transient evaporation method is 66.7 % and 112.9 % smaller than the results of pressure plate measurements, respectively. The underestimation of the two-conductor non-invasive TDR probe in measuring the WRC of loess can be attributed to the fact that the vertical sampling range of two-conductor non-invasive TDR probe is two times greater than that of the tensiometer. The results in this study show the effectiveness of using the non-invasive TDR probe along with a tensiometer for measuring the WRC. More importantly, great caution should be taken on compliant sampling volume of the non-invasive TDR probe and tensiometer to obtain accurate WRC measurements.

Turbulence Measurements with Dual-Doppler Scanning Lidars

Velocity-component variances can be directly computed from lidar measurements using information of the second-order statistics within the lidar probe volume. Specifically, by using the Doppler radial velocity spectrum, one can estimate the unfiltered radial velocity variance. This information is not always available in current lidar campaigns. The velocity-component variances can also be indirectly computed from the reconstructed velocities but they are biased compared to those computed from, e.g., sonic anemometers. Here we show, for the first time, how to estimate such biases for a multi-lidar system and we demonstrate, also for the first time, their dependence on the turbulence characteristics and the lidar beam scanning geometry relative to the wind direction. For a dual-Doppler lidar system, we also show that the indirect method has an advantage compared to the direct one for commonly-used scanning configurations due to the singularity of the system. We demonstrate that our estimates of the radial velocity and velocity-component biases are accurate by analysis of measurements performed over a flat site using a dual-Doppler lidar system, where both lidars stared over a volume close to a sonic anemometer at a height of 100 m. We also show that mapping these biases over a spatial domain helps to plan meteorological campaigns, where multi-lidar systems can potentially be used. Particularly, such maps help the multi-point mapping of wind resources and conditions, which improve the tools needed for wind turbine siting.

Impact of moderate pump–Stokes chirp on femtosecond coherent anti‐Stokes Raman scattering spectra

AbstractThe effects of inducing moderate chirp in the pump and Stokes beams in chirped‐probe‐pulse femtosecond coherent anti‐Stokes Raman scattering (CARS) spectroscopy are discussed. This is an important issue for measurements in high‐pressure systems where the CARS beams must be transmitted through glass of significant thickness to reach the probe volume of interest. The effects were investigated both experimentally, by inserting disks of SF‐11 glass into the pump and Stokes beam paths, and theoretically, by incorporating pulse chirp into our time‐dependent density matrix (TDDM) simulations. Experimentally, we found that inducing moderate pump and Stokes chirp produced significant spectral narrowing of the nonresonant four‐wave mixing contribution to the CARS signal, and this allowed us to control the resonant excitation bandwidth to eliminate interferences from neighboring species. The temperature measurement accuracy and precision were essentially unchanged with respect to the unchirped pump and Stokes case. The effect of moderate pump and Stokes chirp on the narrowing of the Raman excitation efficiency envelope was investigated by solving the full set of TDDM through direct numerical integration. The numerical efficiency of the solutions was enhanced dramatically by developing a parallel version of the TDDM code. The calculated Raman excitation efficiency profile was incorporated in our phenomenological spectral fitting codes.

Spatially resolved thermoreflectance techniques for thermal conductivity measurements from the nanoscale to the mesoscale

Thermoreflectance techniques, namely, time- and frequency-domain thermoreflectance (TDTR and FDTR, respectively), are ubiquitously used for the thermophysical characterization of thin films and bulk materials. In this perspective, we discuss several recent advancements in thermoreflectance techniques to measure the thermal conductivity of solids, with emphasis on the governing length scales and future directions in expanding these advances to different length scales and material structures. Specifically, the lateral resolution of these techniques, typically on the order of several micrometers, allows for an understanding of the spatially varying properties for various materials. Similarly, limitations of TDTR and FDTR with respect to their volumetric probing regions are discussed. With a recently developed steady-state thermoreflectance technique, these limitations are overcome as probing volumes approach spot sizes. Finally, recent pushes toward the implementation of these techniques without the use of a thin metal transducer are presented, with guidelines for future avenues in the implementation under these specimen configurations.

Three-dimensional feedback-driven trapping of a single nanoparticle or molecule in aqueous solution with a confocal fluorescence microscope.

Control of electroosmotic flows in a two-layer microfluidic device with crossed channels is used to counteract Brownian diffusion in aqueous solution for three-dimensional trapping of a single nanoparticle or molecule within the probe volume of a confocal fluorescence microscope. A field programmable gate array sorts and counts photons into four channels synchronous with laser pulses in four beams focused to waists slightly offset from the center of the confocal volume and uses the counts to update voltages between the four fluidic inlets every 13.5 µs. Trapping is demonstrated for 40 nm nanoparticles for up to 240 s, 20 nm nanoparticles for up to 25 s, and single molecules of streptavidin-Alexa 647 for up to 1.2 s.

Pressure-scaling characteristics of femtosecond two-photon laser-induced fluorescence of carbon monoxide.

Broadband femtosecond (fs) two-photon laser-induced fluorescence (TP-LIF) of the B1Σ+←X1Σ+, Hopfield-Birge system of carbon monoxide (CO) is believed to have two major advantages compared to narrowband nanosecond excitation. It should (i) minimize the effects of pressure-dependent absorption line broadening and shifting, and (ii) produce pressure-independent TP-LIF signals as the effect of increased quenching due to molecular collisions is offset by the increase in number density. However, there is an observed nonlinear drop in the CO TP-LIF signal with increasing pressure. In this work, we systematically investigate the relative impact of potential deexcitation mechanisms, including collisional quenching, forward lasing, attenuation of the source laser by the test cell windows or by the gas media, and a 2+1 photoionization process. As expected, line broadening and collisional quenching play minor roles in the pressure-scaling behavior, but the CO fs TP-LIF signals deviate from theory primarily because of two major reasons. First, attenuation of the excitation laser at high pressures significantly reduces the laser irradiance available at the probe volume. Second, a 2+1 photoionization process becomes significant as the number density increases with pressure and acts as a major deexcitation pathway. This work summarizes the phenomena and strategies that need to be considered for performing CO fs TP-LIF at high pressures.

Experimental assessment of effectively probed volume in confocal XRF spectrometry using microparticles

Current approaches for assessing a confocal micro‐X‐rayfluorescence–probing volume involve the use of sharp knife edges, thin films, or wires, which are moved through this volume. The fluorescence radiation excited in the material of the object is measured, and profiles are built to enable the determination of the full width at half maximum in any of the three axes of the excited volume. Such approaches do not provide information on the shape of the volume, and the consequent alignment of both used lenses is made based on the position of the maxima of the registered intensity measurements. The use of particles that are smaller than the interaction volume (isolated enough to prevent the influence of nearby particles) and translated through the interaction volume (3D scan) is presented as an alternative methodology to determine the confocal probing volume. Spherical shaped uranium particles with diameter of 1–3 μm originally produced for scanning electron microscopy analysis calibration purposes were used in this study. The results obtained showed that the effectively probed confocal volume has a distinct prolate spheroidal shape that is longer in the axis of the confocal detector than it is wide on the axes of the plane perpendicular to it. The diameter in the longest axis (tilted accordingly to the angle between the two silicon drift detectors) was found to be approximately 25 μm, whereas the shorter was found about 15 μm each, with a volume of about 3,000 μm3.

Detection of wakes in the inflow of turbines using nacelle lidars

Abstract. Nacelle-mounted lidar systems offer the possibility of remotely sensing the inflow of wind turbines. Due to the limitation of line-of-sight measurements and the limited number of focus positions, assumptions are necessary to derive useful inflow characteristics. Typically, horizontally homogeneous inflow is assumed which is well satisfied in flat, homogeneous terrain and over sufficiently large time averages. However, it is violated if a wake impinges the field of view of one of the beams. In such situations, the turbine yaw misalignment measurements show large biases which require the detection and correction of these observations. Here, a detection algorithm is proposed based on the spectral broadening of the Doppler spectrum due to turbulence within the probe volume. The small-scale turbulence generated within wake flows will typically lead to a significantly larger broadening than in the ambient flow. Thus, by comparing the spectral widths at several measurement locations, situations where a wake is impinging one or more measurement locations can be identified. The correction method is based on an empirical relationship between the difference in turbulence levels at distinct beams and the difference in wind direction derived from the lidar and the real wind direction. The performance of the algorithm is evaluated in a field experiment identifying all wake situations and, thus, correcting the lidar derived wind direction.

Mole fraction measurement through a transparent quarl burner using filtered Rayleigh scattering.

A filtered Rayleigh scattering system is developed and applied to measure the mole fraction of methane in a methane-air swirl flow through a transparent conical quartz quarl. Light scattering from the location where the laser beam is incident on the surface of the quarl is orders of magnitudes larger than Rayleigh scattering from the gas mixture of interest. This diffusive scattering is suppressed using molecular absorption by an iodine cell and using spatial filtering by an optical aperture. Residual stray light accounted for up to 5% of the total signal and had to be removed for accurate measurements. The flow consisted of a nonpremixed mixture of methane and air in the central jet surrounded by a strong swirling air flow. Measurements were conducted at a height of 4 mm from the fuel tube's exit for six different conditions of the swirl flow to demonstrate the ability of the instrument to study the effects of swirl strength and fuel flow rate on the mixing process. By using a four-leg pulse stretcher to allow higher laser energies in the probe volume, large collection optics and a reference iodine cell to monitor laser wavelength variations, standard deviations of ∼0.006 in air and ∼0.012 in a laminar methane flow were achieved for mole fraction measurements.

Investigating temporal variation in the apparent volume fraction measured by time-resolved laser-induced incandescence

In many time-resolved laser-induced incandescence (TiRe-LII) experiments, it is common practice to relate the intensity emitted by laser-heated nanoparticles to the detected LII signal through a factor (here called the intensity scaling factor, ISF) that includes the particle volume fraction and other parameters that may not be the focus of the analysis. While, in the absence of evaporation or sublimation, the ISF should theoretically remain constant with respect to time, recent multi-wavelength measurements show that, in reality, it may vary with both time and fluence. We consider four candidate effects that contribute to this behavior: particle annealing; polydispersity in the nanoparticle-size distribution; background luminosity due to emission from nanoparticles in the line-of-sight before and behind the probe volume; and the temporal resolution of the detector. We demonstrate these effects by simulating TiRe-LII data for in-flame soot at atmospheric pressure, using new simplified heat transfer and annealing models. Analysis of experimental signals collected from flame-generated soot at atmospheric pressure reveals trends in the ISF similar to those predicted by simulations. These temporal variations provide important insights that can help to diagnose problems in TiRe-LII experiments and improve TiRe-LII models.

Robust Lidar Data Processing and Quality Control Methods Developed for the SWiFT Wake Steering Experiment

Sandia National Laboratories and the National Renewable Energy Laboratory conducted a wake-steering field experiment at the Scaled Wind Farm Technology facility. The campaign included the use of two highly instrumented V27 wind turbines, an upstream meteorological tower, and high-resolution wake measurements of the upstream wind turbine using a customized scanning SpinnerLidar from the Technical University of Denmark. The present work details how the SpinnerLidar data uploaded to the Department of Energy Atmosphere to Electrons Data Archive and Portal was processed, quality controlled and assured to guarantee high data availability with the removal of invalid measurements. A multidimensional approach to processing the SpinnerLidar Doppler spectra was developed based on matching erroneous measurements within the two-dimensional lidar scan with patterns inside the multidimensional lidar Doppler spectra. This method allows image processing techniques to be used to remove regions of the Doppler spectra that are contaminated by hard targets and isolate the velocity field of interest, allowing more accurate line-of-sight velocity measurements and enabling the estimation of the turbulence of the line-of-sight velocities within the probe volume.

Investigation of the Specific Retention Volume of the Probe Volume and the Effects on the Polymer-Probe System by Inverse Gas Chromatography

In this study, the effects of probe quantities on retention volume and the physical and thermodynamic results of polymer-probe systems were investigated. For this purpose, by using inverse gas chromatographic method. Alcohols and alkanes with different chemical and physical properties were injected as probes on homopolymer (2-cyclohexylidene-1,3-dioxolane-4-yl-methyl methacrylate) (CHMMA). Probe quantities of 0.3, 0.6, and 0.9 μl were selected, and an injection was made at every 10°C between 40 and 150°C. In addition, 3 μl volume probes were tried but reproducible results were not obtained in these volumes and the detector was observed to be out of order after several injections. It has been observed that the specific retention volume of alcohols and alkanes partially increased by increasing the injection amount. A linear relationship was observed between probe quantities and specific retention volume. This linear relationship is apparent from the specific retention volume values, where the probes are independent of the physical and chemical structures. It was observed that the results obtained in all three injections were close to each other and within acceptable limits. The glass transition temperature of the polymer was determined to be a Tg of 60°C. The thermodynamic data calculated for the injection of different amounts of probes were close to each other.

Temperature and water measurements in flames using 1064 nm Laser-Induced Grating Spectroscopy (LIGS)

Laser-Induced Grating Spectroscopy (LIGS) is applied to premixed CH4/air laminar flat flames under operating pressures of 1 to 6 bar. For the first time, temperature and water concentration have been acquired simultaneously in a reacting flow environment using LIGS. A 1064 nm pulsed laser is used as pump to generate a temporary stationary intensity grating in the probe volume. Water molecules in the flame products absorb the laser energy and generate a thermal grating if sufficiently high energies are delivered by the laser pulses, here more than 100 mJ per pulse. Such energies allow the electric field to polarize the dielectric medium, resulting in a detectable electrostrictive grating as well. This creates LIGS signals containing both the electrostrictive and the thermal contributions. The local speed of sound is derived from the oscillation frequency of LIGS signals, which can be accurately measured from the single shot power spectrum. Data show that the ratio between the electrostrictive and the thermal peak intensities is an indicator of the local water concentration. The measured values of speed of sound, temperature, and water concentration in the flames examined compare favorably with flame simulations with Chemkin, showing an estimated accuracy of 0.5 to 2.5% and a precision of 1.4–2%. These results confirm the potential for 1064 nm LIGS-based thermometry for high-precision temperature measurements of combustion processes.

A highly sensitive assay of DNA based on inductively coupled plasma mass spectrometry detection with gold nanoparticle amplification and isothermal circular strand-displacement polymerization reaction

In this work, a simple, sensitive and specific assay for DNA was proposed by combining inductively coupled plasma mass spectrometry (ICP-MS) detection with gold nanoparticle (AuNPs) amplification and isothermal circular strand-displacement polymerization reaction (ICSDPR). First, AuNPs were decorated with hairpin-structured DNA (HP-DNA) through Au–S bond to form the AuNPs probe. The ICSDPR was conducted on AuNPs probe in a homogeneous phase to realize the dual amplification and simplify the analytical process at the same time. By using a biotin modified primer, AuNPs were connected with biotins after the ICSDPR, then captured by the streptavidin modified magnetic beads (SA-MBs), and finally detected by ICP-MS. Many key factors including probe volume, hybridization time, polymerase amount, primer concentration, enzyme reaction time, SA-MBs capture time and desorption time were optimized. Under the optimized condition, the proposed method could detect target DNA as low as 45 zmol (8.9 fM in 5 μL) in a relative short time (about 4.5 h) with good specificity, and the linear range of this method is 0.1–10000 pM, the relative standard deviations are in the range of 3.6–6.4%. The proposed method was applied for determination of target DNA in human serum samples, the recovery for the spiked human serum samples is in the range of 84–120%. It demonstrates a good application potential of the developed method for biological studies and clinical diagnosis of human pathogenic diseases.

Optimizing Measurement of Vascular Endothelial Growth Factor in Small Blood Samples of Premature Infants

To establish a method that allows for the reliable assessment of vascular endothelial growth factor (VEGF-A) concentrations in very small blood samples of preterm infants. Systemic VEGF measurements are important in view of the most appropriate Anti-VEGF drug to be used for the treatment of acute retinopathy of prematurity (ROP). Cord blood samples from preterm (n = 6) infants, blood samples from preterm infants with treatment requiring ROP (n = 12), and blood samples from healthy adults (n = 10) were collected. Serum, citrate plasma, and serum from recalcified citrate blood were obtained. Levels of VEGF-A and platelet factor-4 (PF-4) were quantified by ELISA or AlphaLISA immunoassay. VEGF-A levels could be detected by both assays, with the AlphaLISA generating slightly lower levels in healthy adults, but not in cord blood of preterm infants. In plasma samples, VEGF levels ranged from non detectable to 181 pg/ml. PF-4 concentrations were between 0.16–3.88 µg/ml. Values of VEGF-A and PF-4 in serum and recalcified serum were significantly higher compared to plasma through the release of these cytokines after platelet activation. In plasma samples of infants with ROP, VEGF-A could always be detected and its values ranged from 19.50 to 245.91 pg/ml and PF-4 concentrations were between 0.1 and 3.3 µg/ml. Using the AlphaLISA kit, we were able to detect VEGF in small sample volumes (5 µl plasma or serum/well) in premature infants with treatment requiring ROP and to monitor platelet activation by PF-4 detection. Minimal blood probe volumes reduce phlebotomy losses avoiding the risk of iatrogenic anemia, thus allowing close monitoring of the cytokine levels in these very fragile infants.

Inline Reaction Monitoring of Amine-Catalyzed Acetylation of Benzyl Alcohol Using a Microfluidic Stripline Nuclear Magnetic Resonance Setup.

We present an in-depth study of the acetylation of benzyl alcohol in the presence of N,N-diisopropylethylamine (DIPEA) by nuclear magnetic resonance (NMR) monitoring of the reaction from 1.5 s to several minutes. We have adapted the NMR setup to be compatible to microreactor technology, scaling down the typical sample volume of commercial NMR probes (500 μL) to a microfluidic stripline setup with 150 nL detection volume. Inline spectra are obtained to monitor the kinetics and unravel the reaction mechanism of this industrially relevant reaction. The experiments are combined with conventional 2D NMR measurements to identify the reaction products. In addition, we replace DIPEA with triethylamine and pyridine to validate the reaction mechanism for different amine catalysts. In all three acetylation reactions, we find that the acetyl ammonium ion is a key intermediate. The formation of ketene is observed during the first minutes of the reaction when tertiary amines were present. The pyridine-catalyzed reaction proceeds via a different mechanism.

High resolution LDA measurements in transitional oblique shock wave boundary layer interaction

Spatial development of a transitional Oblique Shock Wave Interaction at Mach 1.68 is presented. This type of flow is characterised by very small length scales (boundary layer thickness is smaller than 1mm), high velocities, reverse flows and a wide range of velocity fluctuations along the transition process. Unsteady velocity fields have been obtained using a high spatial resolution Laser Doppler Anemometry system, allowing quantitative measurements of the velocity fluctuations down to $$y/\delta =0.1$$ . A model to take into account the finite size of the probe volume on the mean and RMS velocity measurements is used and applied to the present measurements. Finally, the amplification of the velocity fluctuations along the transitional separated shear layer is described.

Status and nutritional therapy in elective and emergency neurosurgery patients.

To evaluate the Nutritional Status (NS) and follow the Enteral Nutritional Therapy (ENT) of patients in neurosurgical intervention. Cohort study in emergency or elective surgery patients with exclusive ENT. Anthropometric measurements (Arm Circumference (AC and Triceps Skinfold (TSF)) were measured on the first, seventh and 14th day. For the ENT monitoring, caloric/protein adequacy, fasting, inadvertent output of the enteral probe and residual gastric volume were used. 80 patients, 78.7% in emergency surgery and 21.3% in elective surgery. There was a reduction in AC and Body Mass Index (BMI) (p>0.01), especially for the emergency group. The caloric/protein adequacy was higher in the emergency group (86.7% and 81.8%). The EN change was greater in the emergency group, even with better ENT adequacy. Changes in body composition are frequent in neurosurgical patients, regardless of the type of procedure.

Single Layer Graphene for Estimation of Axial Spatial Resolution in Confocal Raman Microscopy Depth Profiling.

Single layer graphene (SLG), with its angstrom-scale thickness and strong Raman scattering cross section, was adapted for measurement of the axial ( Z-direction) probe beam profile in confocal Raman microscopy depth-profiling experiments. SLG adsorbed to a glass microscope coverslip (SLG/SiO2) served as a platform for the estimation of axial spatial resolution. Profiles were measured by stepping the confocal probe volume through the SLG/SiO2 interface while measuring Raman scattering from the sample. Using a high numerical aperture (1.4 NA) oil immersion objective, axial profiles were derived from the graphene 2D vibrational mode and fit to a Lorentzian instrument response function (IRF). Subsequently, the Z-direction spatial resolution in depth-profiling studies of polymer interfaces was estimated through convolution of the Lorentzian IRF with a step function representing the ideal junction separating the phases of interest. In the study of a bipolar polymer membrane, confocal Raman depth profiles of the AEM/CEM (anion exchange membrane/cation exchange membrane) interface show that the transition region is broader than the limiting response and are consistent with roughness at the boundary on the order of a few micrometers. Using ClO4- as a Raman active mobile ion probe, application of self-modeling curve resolution (SMCR) to spectral data sets within a profile showed ClO4- ions track the spatial distribution of the AEM phase. Finally, in measurements on a liquid-solid interface formed between 1-octanol and a polydimethylsiloxane (PDMS) membrane, the IRF derived from fitting the experimental profile was slightly narrower than those obtained from profiling SLG, indicating the potential to use polymer-liquid interfaces formed from widely available materials and reagents for estimation of axial spatial resolution in confocal Raman depth-profiling.