Vertical Shoot Position Research Articles

Effects of grapevine canopy leaning on grape composition and wine quality of ‘Bobal’

Exploring new field strategies for adapting winegrowing regions to the adverse effects of climate change becomes imperative. This study focuses on the effects of canopy architecture through the trellis systems leaning of North–South oriented vineyard’s rows vertical shoot positioned (VSP) system by 30° towards either the East (ESP) or the West (WSP) as a possible adaptation technique. The trial was conducted over two seasons in a Vitis vinifera L. cv. Bobal vineyard located in Requena (Valencia), eastern Spain, under a temperate-warm climate. The canopy leaning was considered to affect the radiation load and its timing with potential effects on vine physiology and grape metabolism and, consequently, on grape and wine quality. Leaning treatments affected vegetative growth, likely due to slight differences in vine water status. In comparison to VSP and ESP, WSP intercepts more light in the morning, when the photosynthetic capacity of the vines is at its highest. However, this results in a more negative vine water potential, which in turn leads to a reduction in vigour with no impact on yield. Musts from the WSP showed a tendency to increase total acidity compared to VSP, while ESP tended to decrease it. This was likely due to the reduction in temperature within the cluster microclimate in WSP in comparison to the ESP and VSP treatments. In addition, musts from the WSP treatment showed higher colour intensity, anthocyanins and polyphenols, potentially attributable to its more negative potentials, likely due to its more negative potentials, which may have prompted the synthesis of phenolic compounds. Consequently, wines from the WSP treatment displayed higher total acidity and lower pH, as well as higher colour intensity, anthocyanins, and polyphenolic content. In addition, WSP wines had higher concentrations of esters and higher alcohols. The improvements in grape and wine composition are likely due to an increased cluster radiation interception in the morning and reduced heating in the afternoon when VPD is higher. Our findings demonstrate that canopy management by leaning VSP vines towards the West can be a useful technique for adapting grape and wine composition to climate change and provide insights into its physiological basis.

Canopy Architecture and Sun Exposure Influence Berry Cluster-Water Relations in the Grapevine Variety Muscat of Alexandria.

Climate-change-related increases in the frequency and intensity of heatwaves affect viticulture, leading to losses in yield and grape quality. We assessed whether canopy-architecture manipulation mitigates the effects of summer stress in a Mediterranean vineyard. The Vitis vinifera L variety Muscat of Alexandria plants were monitored during 2019-2020. Two canopy shoot-positioning treatments were applied: vertical shoot positioning (VSP) and modulated shoot positioning (MSP). In MSP, the west-side upper foliage was released to promote partial shoot leaning, shading the clusters. Clusters were sampled at pea size (PS), veraison (VER), and full maturation (FM). Measurements included rachis anatomy and hydraulic conductance (Kh) and aquaporins (AQP) and stress-related genes expression in cluster tissues. The results show significant seasonal and interannual differences in Kh and vascular anatomy. At VER, the Kh of the rachis and rachis+pedicel and the xylem diameter decreased but were unaffected by treatments. The phloem-xylem ratio was either increased (2019) or reduced (2020) in MSP compared to VSP. Most AQPs were down-regulated at FM in pedicels and up-regulated at VER in pulp. A potential maturation shift in MSP was observed and confirmed by the up-regulation of several stress-related genes in all tissues. The study pinpoints the role of canopy architecture in berry-water relations and stress response during ripening.

Pneumatic spray delivery-based fixed spray system configuration optimization for efficient agrochemical application in modern vineyards.

The hydraulic spray delivery (HSD)-based solid set canopy delivery system (SSCDS) emitter configuration has been optimized for agrochemical applications in vertical shoot position (VSP) vineyards. It uses cost-prohibitive emitters, and their placement restricts the mechanical pruning activities. Therefore, this study focused on optimizing the spray performance of a pneumatic spray delivery (PSD)-based SSCDS variant that addresses the earlier issues. Three PSD-SSCDS emitter configurations (C1-C3) were designed using modified low-cost emitters (E1: modified flat fan, E2: 90° modular flat fan) for agrochemical applications in VSP vineyards. C1 had an E1 installed on trellis posts at 1.67 m above ground level. C2 had a pair of E2 installed per vine at 0.3 m below the cordon, while C3 combined the emitter placement of C1 and C2. The spray deposition (ng cm-2) and coverage (%) were quantified (mean ± standard error) using mylar cards and water-sensitive paper samplers placed within the canopy, respectively. Spray deposition for C1, C2, and C3 was 301.12 ± 63.30, 347.9 ± 66.29, and 837.6 ± 92.53 ng cm-2, respectively. Whereas spray coverage for corresponding configurations was 18.02 ± 2.63, 8.98 ± 1.84, and 28.84 ± 2.46%, respectively. Overall, configuration C3 provided significantly higher spray deposition and coverage than C1 and C2. Substantially reduced system installation cost and emitter density per hectare with improved spray performance were achieved by C3 compared to earlier optimized HSD-SSCDS configuration in the VSP vineyards. © 2024 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Physiological efficiency of grapevine canopies having varying geometries: Seasonal and diurnal whole canopy gas exchange assessment under well-watered and water deficit conditions

Grapevine training systems should be thoroughly examined to contribute to vineyards more resilient to climate change. This study was conducted on 18 three-year-old fruiting Sangiovese vines grown in pots outdoors with canopies trained to a vertical shoot positioned (VSP) spur-pruned cordon, a single high wire (SHW), and a Pergola (P) training system. Whole-canopy net CO2 exchange rate (NCER) and transpiration (T) were monitored by an enclosure system on a 24/7 basis from DOY 179–249. All vines were kept well-watered (WW) from DOY 179 to DOY 199, and the water supply was first reduced to 50% (DOY200–204) and then to 35% (DOY 205–214) before final rewatering (DOY 215–217). Vegetative growth, yield components, and grape composition were also assessed. Seasonal gas exchange on a per vine basis reflected vine size given as total leaf area (LA) varying from 2.5 to 3.1 m2; however, when diurnal trends of NCER/LA and T/LA within the WW and rewatering periods were considered, the good correlations found with the modeled total canopy light interception (TCLI) in SHW and P, were not confirmed in VSP. In all canopy geometries, the best predictor of NCER/LA was the total direct light intensity, whereas T/LA was linearly and closely correlated with air VPD. In terms of NCER/LA during the WW period, SHW had 24% higher rates than VSP and P. Under progressive water stress, SHW again exhibited higher drought resilience, while NCER and T were severely reduced in VSP and P. Severe stress also caused lower light saturation points and quantum yield in VSP and P. Under similar yield levels, P reached the best fruit maturity. In terms of photosynthetic efficiency and adaptive response to increasing water stress, the SHW performed best. Results extrapolated at the field level indicate the P system as the most rewarding.

Climate Change Affects Choice and Management of Training Systems in the Grapevine

Although vertical shoot positioned (VSP) training systems, either cane- or spur-pruned, are adopted in the great majority of the vineyards worldwide, the lianas nature of the grapevine and the presence of long and flexible canes confer high plasticity and render structural and pruning changes quite easy. The focus of this review is if, in light of the most consistent features triggered by global warming (e.g., longer growing season, earlier phenology, faster ripening, higher incidence of overheating stress and sunburn, higher frequency of extreme weather events), the type and management of training systems should also be reconsidered. We surveyed the main methods to assess training system efficiency and the current attempts and outlook toward exploiting the training system as an adaptation tool to climate change. For the latter, we considered 12 main trellis types and scored them based on climate-related features and general traits such as vigor, yield control, susceptibility to fungal diseases, and suitability according to wine types (still or sparkling). The resulting balance of positive and negative recommendations leads to a re-evaluation of either old, nonmechanizable trellis types (e.g., Raggi-Bellussi and pergola types), divided canopy systems (e.g., GDC and Scott Henry) or, among the single canopy types, of the single high wire (SHW) trellis. However, historical systems traditionally used by best regions and producers (e.g., goblet and VSP either cane- or spur-pruned) overall show less adherence to the chosen evaluation criteria. To direct future evolution of training systems, regardless of the broadly shared need for suitability to partial or full mechanization, the scenario looks different depending on cool and temperate (warm) areas. The former experiences an outburst of interest as warming is broadening growing areas and affordable genotypes. Under such circumstances, training systems should help accelerate or favor the ripening process through vigor control and lower yield, better cluster exposure, and nonlimiting leaf area-to-fruit ratio. Whereas, in warm areas that are now becoming sub-tropical areas in the worst cases, the SHW gains credit as compared to goblet and traditional VSP. The latter requires an increasing number of canopy manipulations and a rethinking of some planting choices to accommodate the needs of slower and more delayed ripening, more cluster shading, and higher cordons, the latter reducing the probability of incurring significant frost damage.

Open-Format Prescription Maps for Variable Rate Spraying in Orchard Farming

Highlights Straightforward procedure to code prescription maps independent of manufacturers. Methodology suitable for orchard crops and vineyards including pressures up to 10 bar and flowrates up to 100 L/min. Algorithm validated for both trellis-based continuous rows and traditional orchard rows of discrete trees. Method scalable to other agricultural operations, such as fertilizing or data registration for auditing purposes. Abstract. The implementation of crop protection solutions contributing to significant reductions in the use and risk of chemical pesticides has motivated the interest of site-specific systems, such as variable rate technologies based on user-set prescriptions. However, the practical implementation of variable rates has been dissuasive for many growers who are not familiar with digital solutions, as proprietary firmware is often difficult to grasp. This paper presents a method for formatting prescription maps that is independent of manufacturers, can be easily and freely implemented by industry and can be adopted by growers not familiar with advanced software tools. The methodology was successfully demonstrated for perennial specialty crops, in particular for trellised vineyards in vertical shoot position and for traditional olive groves. Results showed that the variations within user prescription maps were translated to the fields, but flow errors appeared due to the challenge of measuring flow and pressure in the highly pulsating actuation of pulse-width-modulated (PWM) valves. Such errors diminished with the use of low-volume rates and the optimal selection of the nozzles when adjusted to the real pressure measured in the field. The use cases validated in the field showed the consistency of the method for highly populated prescription maps of 1,221 points/ha accessible to advanced farmers, as well as for modest maps of 80 points/ha within reach of the majority of growers. Keywords: Automated map building, Local tangent plane coordinates, Orchard crop operations, Precision farming, Prescription maps, User-centered approach, Variable rate spraying.

Consistent benefits of fungicide reduction on arthropod predators and predation rates in viticulture: a five-year experiment

For reliable pest suppression, benefits of habitat management for natural enemies of agricultural pests need to be consistent over time. Unfortunately, most research projects allow only for one or two years of data collection. Here, we present a five-year study on effects of fungicide reduction and altered plant architecture on arthropod abundances and natural pest control in an experimental vineyard. The vineyard rows were divided into eight groups, half of which were trained in vertical shoot position (“trellis system”) and the other half as semi-minimal pruned hedge (“minimal pruning”). Every row was divided in three sections receiving three different plant protection intensities, respectively, with fungicides certified for organic viticulture. In each year we sampled arthropods from the grapevine canopy by standardized leaf collection and beat-sheet sampling, and exposed baits of a major grapevine pest (Lobesia botrana) to assess natural pest control. Arthropods, in particular predators, benefited from reduced fungicide sprayings and in turn promoted natural pest control. In contrast, effects of minimal pruning were less strong, and restricted to the leaf mesofauna, earwigs and leafhoppers. Across the five study years with their variable weather conditions, we conclude that the advantages of reduced fungicide sprayings in fungus-resistant varieties are consistent over time.

Cluster microclimate, canopy management and its influence on the berry (size and composition) quality

Sunlight and microclimate inside the clusters’ zone are key factors in berry development and must composition. Plant geometry and training system should be joined with a proper sunlight and temperature cluster microclimate and, also in the rest of the plant. Berry temperature can vary between 2 and 10 ºC or even more in inner clusters, depending on their exposure (Spayd et al., 2002). Sunlight, air ventilation within the canopy, temperature cluster and microclimate are affected by the exposure and radiation percentage received by grapes during its growth and maturation period (Deloire and Hunter 2005). In Mediterranean conditions (warm and dry climate), the use of porous systems may help plants establish a better leaf distribution inside this area (de la Fuente et al., 2015), providing more space and enhancing certain physiological processes, both in leaves (photosynthesis, ventilation, transpiration) and berries (growth and maturation). Grapes exposed to direct radiation are more sensitive to ripening and they can even suffer a dehydration process in the Mediterranean regions, where the temperature during the ripening after midday is frequently between 30-35 ºC or higher than 40 ºC (de la Fuente et al., 2015). A key point for well microclimate management inside the canopy is heat flux control, which is usually generated by three factors: surface area (SA) to PAR (direct or indirect) radiation; intensity or thermal value (related to the temperature) and time of exposure (de la Fuente, 2009; de la Fuente et al., 2013). Sprawl systems are non-positioned systems where vegetation is in multidirectional directions. Therefore, as sun position changes along the day, some leaves are first shaded and then others, so the sun leaf exposure decreases (Gutiérrez et al., 2021). The study was conducted in D.O. Uclés vineyards (lat. 39º50’8” N; long. 3º09’48.6” W; elevation 746 m above sea level) during the 2020 season, in cv. Tempranillo. The trial was designed with two training system: Vertical Shoot Positioned (VSP) and Sprawl (SP). Berry sampling was done every three days within the final 15 days before the estimated harvest date. A single sample comprised 100 berries collected from the clusters of the 10 selected vines in each block. Weight, size, must composition (reducing sugar, pH, acidity, volatile acidity, etc.) and skin composition (total and acylated monomeric pigments, TPI) were analysed. Regarding the berry composition, SP accumulated larger concentration of reducing sugars (+7.4%). No statistical differences were observed in the remaining oenological parameters measured in the berries between treatments. Nevertheless, inside total pigments and color parameters, TPI values reached significantly higher (+40%) in the SP vs VSP treatments. In the total concentration of pigments (including the acylated fraction) and pH, no differences were founded. Berry's weight and size showed some relevant differences between treatments. During the end maturity-harvest period, the berry weight (12-11%) and berry size (6-9%) were higher in SP treatment compared to VSP. These results suggest that the SP systems can induce an increment of reducing sugars, and TPI and also, can modulate the berry weight and size, helping to control overripening and berry dehydration processes. Therefore, sprawl systems (SP) represent an alternative to VSP systems in warm areas for achieving an increment of pigments, as well as for better control of the accumulation of reducing sugars, without compromising the harvest yield (higher berry weight and size).

Responses of grapevines to planting density and training systems in semiarid environments

Aim. To investigate the physiological and agrobiological responses of grapevines on different systems of training systems in row plantations depending on the planting density and the possibility of adjusting plants for non-irri- gated crop cultivation under cold semiarid climate. Methods. The field two-way experiment of 2 × 4 was applied to study the effect of two planting density variants (3 × 1.5 m; 3 × 1 m) and four vine training systems (when the horizontal cordon is located at the height of 0.4 m, 0.8 m, 1.2 m, 1.6 m) on the parameters of the leaf area, leaf index, water loss through transpiration, activity of the photosynthetic apparatus of leaves and the yield of Zahrei wine grape cultivar (Vitis vinifera L.). The experiment data were processed using the analysis of variance. Results. The increase in the density of vine row plantations per area units from 3 × 1.5 m to 3 × 1 m enlarges the total leaf area, the exposed leaf area, water loss through transpiration, and yield weight, but these indices decrease in the evaluations per plant. The responses of grapevine to the training systems are similar and independent from the ex- perimental variants of planting density. At the height of the cordon of 0.4 and 0.8 m, the vertical shoot positioning enhances the potential of the photosynthetic capacity of the plantations, but this potential is realized only during the years with lower water deficit for plants. Free-growing shootings on the cordons, located at the height of 1.2 m, form the canopy architecture with relatively low water loss through transpiration which has a positive effect on the activity of the photosynthetic apparatus and yield, especially in dry years. The downward shoot positioning on the cordons of 1.6 m decreases the leaf area of the vines and creates the canopy architecture with increased transpiration which enhances the effect of the water deficit and has a negative effect on the productivity of plants. Conclusions. The agronomic methods of planting and training systems for grapevines ensure the management of the character of spatial shoot location, the formation of certain canopy architecture, and setting the parameters of the leaf area; their optimization mitigates the negative effect of water deficit and provides for adapting the plants for non-irrigated crops under semiarid climate. The positive effect of compacting plantations on crop yield was determined without irrigation in semiarid environments. The variant of planting density of 3 × 1 m decreases the yield on the vines on average by 12.1–31.0 %, as compared to the variant of 3 × 1.5 m. Yet, more dense plantations are remarkable for their yield, which is 18.5–61.3 % higher depending on the training system for vines. Under dry conditions, the most efficient system is the training system with the formation of the horizontal cordon at the height of 1.2 m and free-growing shoots. The system optimizes the leaf area density, and forms the canopy architecture with rather low water loss through transpiration which has a positive effect on the activity of the photosynthetic apparatus of leaves during droughts. Under free growth, the yield of the plantations increases by 4.3–12.3 % on average as compared to the vertical shoot positioning and by 21.3 % – under their downward positioning.

Transcriptome and metabolome reveal the effects of three canopy types on the flavonoids and phenolic acids in ‘Merlot’ (Vitis vinifera L.) berry pericarp

The flavonoids and phenolic acids in grape berries greatly influence the quality of wine. Various methods are used to shape and prune grapevines, but their effects on the flavonoids and phenolic acids remain unclear. The flavonoids and phenolic acids in the berry pericarps from grapevines pruned using three types of leaf canopy, namely, V-shaped, T-shaped, and vertical shoot-positioned (VSP) canopies, were compared in this study. Results showed that the V-shaped canopy was more favorable for the accumulation of flavonoids and phenolic acids. Transcriptome and metabolome analyses revealed that the differentially expressed genes (DEGs) and differentially regulated metabolites (DRMs) were significantly enriched in the flavonoid and phenylpropanoid biosynthesis pathways. A total of 96 flavonoids and 32 phenolic acids were detected among the DRMs. Their contents were higher in the V-shaped canopy than in the T-shaped and VSP canopies. Conjoint analysis of transcriptome and metabolome showed that nine DEGs (e.g., cytochrome P450 98A9 and 98A2) were significantly correlated to nine phenolic acids (e.g., gentisic acid and neochlorogenic acid) and three genes (i.e., chalcone isomerase, UDP-glycosyltransferase 88A1, and caffeoyl-CoA O-methyltransferase) significantly correlated to 15 flavonoids (e.g., baimaside and tricin-7-O-rutinoside). These genes may be involved in the regulation of various flavonoids and phenolic acids in grape berries, but their functions need validation. This study provides novel insights into the effects of leaf canopy on flavonoids and phenolic acids in the skin of grape berries and reveals the potential regulatory networks involved in this phenomenon.

Adapting wine grape production to climate change through canopy architecture manipulation and irrigation in warm climates.

Grape growing regions are facing constant warming of the growing season temperature as well as limitations on ground water pumping used for irrigating to overcome water deficits. Trellis systems are utilized to optimize grapevine production, physiology, and berry chemistry. This study aimed to compare 6 trellis systems with 3 levels of applied water amounts based on different replacements of crop evapotranspiration (ETc) in two consecutive seasons. The treatments included a vertical shoot position (VSP), two modified VSPs (VSP60 and VSP80), a single high wire (SH), a high quadrilateral (HQ), and a Guyot pruned VSP (GY) combined with 25%, 50%, and 100% ETc water replacement. The SH had greater yields, whereas HQ was slower to reach full production potential. At harvest in both years, the accumulation of anthocyanin derivatives was enhanced in SH, whereas VSPs decreased them. As crown porosity increased (mostly VSPs), berry flavonol concentration and likewise molar % of quercetin in berries increased. Conversely, as leaf area increased, total flavonol concentration and molar % of quercetin decreased, indicating a preferential arrangement of leaf area along the canopy for overexposure of grape berry with VSP types. The irrigation treatments revealed linear trends for components of yield, where greater applied water resulted in larger berry size and likewise greater yield. 25% ETc was able to increase berry anthocyanin and flavonol concentrations. Overall, this study evidenced the efficiency of trellis systems for optimizing production and berry composition in Californian climate, also, the feasibility of using flavonols as the indicator of canopy architecture.

Improvement of biogenic amines in grape juice from Vitis labrusca and hybrid grapes grown in different training systems

ABSTRACT Bioactive amines present in grapes and their derivatives are used as quality markers and have important physiological functions in plants and humans. We quantified the biogenic amines and antioxidant activity in whole juices of Vitis labrusca and hybrid grapes developed in Brazil. The grapes used were V. labrusca (‘Bordô’ and ‘Isabel’) and Brazilian hybrids (‘IAC 138–22 Maximo’ and ‘BRS Violeta’) grafted onto ‘IAC 766 Campinas’ rootstock. All vines were trained in low and high vertical shoot positions. The training systems and genotypes used were highly related to the presence of amino compounds and antioxidants. The juices from ‘Bordô’, ‘IAC 138–22 Maximo’ and ‘BRS Violeta’ grapes presented higher contents of beneficial biogenic amines. All juices contained biogenic amines at considerable levels and, consequently, had high biological potential. A high vertical shoot position system is recommended to improve dopamine, melatonin, and serotonin levels.

Crop coefficients (Kc) developed from canopy shaded area in California vineyards

Seasonal grapevine crop coefficients (Kc) were estimated from the shaded area (SA) beneath grapevine canopies at solar noon in vineyards with different trellises and row spacings. The trellises included the vertical shoot positioning (VSP) trellis, Lyre trellis, single and double curtains, cross-arms of varying lengths, and sprawl systems (shoots not positioned). Measurements were taken throughout the grape growing regions of California. The percent shaded area (PSA) was multiplied by 0.017, slope of the relationship between PSA and Kc, to calculate the Kc. Vines used to measure SA were not stressed for water as midday leaf water potential was ≥ −1.0 MPa. The seasonal increase in SA was significantly correlated with the accumulation of degree-days (DDs; base 10°C from the approximate date of budbreak) across locations and years. The PSA was a significant function of trellis – wider trellises spread the canopy and increased SA more than narrower trellises, thus having greater Kc values. The mid-season Kc (Kc mid) for the Lyre and VSP trellises on a 2.74 m row spacing were 0.96 and 0.49, respectively. In addition, Kc mid was inversely correlated with vineyard row spacing – the closer the row spacing for a particular trellis, the greater the Kc mid. The Kc mid for a VSP trellis on 1.83 and 3.05 m row spacings were 0.87 and 0.52, respectively. In contrast, the effects of vineyard row direction and location were minor. The seasonal Kc values developed here do not decrease from mid-season, even after harvest, with a few exceptions. Equations given herein express the seasonal Kc as a function of degree-days, trellis type, and row spacing, thus providing useful estimates of seasonal Kc values for use in California or elsewhere in vineyards with trellises like those in this study.

3D Assessment of Vine Training Systems Derived from Ground-Based RGB-D Imagery

In the field of computer vision, 3D reconstruction of crops plays a crucially important role in agriculture. On-ground assessment of geometrical features of vineyards is of vital importance to generate valuable information that enables producers to take the optimum actions in terms of agricultural management. A training system of vines (Vitis vinifera L.), which involves pruning and a trellis system, results in a particular vine architecture, which is vital throughout the phenological stages. Pruning is required to maintain the vine’s health and to keep its productivity under control. The creation of 3D models of vineshoots is of crucial importance for management planning. Volume and structural information can improve pruning systems, which can increase crop yield and improve crop management. In this experiment, an RGB-D camera system, namely Kinect v2, was used to reconstruct 3D vine models, which were used to determine shoot volume on eight differentiated vineyard training systems: Lyre, GDC (Geneva Double Curtain), Y-Trellis, Pergola, Single Curtain, Smart Dyson, VSP (Vertical Shoot Positioned), and the head-trained Gobelet. The results were compared with dry biomass ground truth-values. Dense point clouds had a substantial impact on the connection between the actual biomass measurements in four of the training systems (Pergola, Curtain, Smart Dyson and VSP). For the comparison of actual dry biomass and RGB-D volume and its associated 3D points, strong linear fits were obtained. Significant coefficients of determination (R2 = 0.72 to R2 = 0.88) were observed according to the number of points connected to each training system separately, and the results revealed good correlations with actual biomass and volume values. When comparing RGB-D volume to weight, Pearson’s correlation coefficient increased to 0.92. The results reveal that the RGB-D approach is also suitable for shoot reconstruction. The research proved how an inexpensive optical sensor can be employed for rapid and reproducible 3D reconstruction of vine vegetation that can improve cultural practices such as pruning, canopy management and harvest.

Evolution of Volatile Aroma Compounds and Amino Acids in Cabernet Gernischt Grape Berries (Vitis vinifera L.): Comparison of Different Training Systems for Mechanical Soil Burial.

Soil burial is a survival strategy for grapevines that can safely overwinter in north-western regions of China. A suitable training system was beneficial for soil burial to provide winter protection. Moreover, the training system can also significantly affect fruit quality during the development of grape berries, such as primary and secondary metabolites. In this study, four-year-old Cabernet Gernischt grapevines were used as experimental material and exposed to four training systems, including the Ningxia traditional vertical trunk (control, CK); the gobelet (T1); single guyot (T2); slant trunk with vertical shoot positioning (STVSP) (T3). The results showed that total soluble solid total phenol content was 12.69%, 57% higher under T3 training systems than in the control, and T3 alleviated the canopy density, leading to improving the leaf photosynthetic efficiency gas chromatography-mass spectrometry (GC-MS) assay used to detect the aroma compounds. The results indicated that the T3 training system enhanced the accumulation of alcohols, carbonyl compounds, C6/C9 and esters, which account for the largest proportion of volatile compounds, and the qRT-PCR reveals that VvEcar, Vvter, VvCCD1, and VvLis were raised under T3 at the transcriptional level. Moreover, T3 contributes to most free amino acid synthesis. Additionally, the PCA reveals the correlation of free amino acids under four training systems, which reflected the mostly amino acid related to T3, and thus, we could speculate that T3 enhances the overall aroma. These results may lead to new strategies to select a new, short trunk training system to achieve mechanized buried soil, to prevent cold and produce high-quality wine in this area.

Minimal versus Intensive: How the Pruning Intensity Affects Occurrence of Grapevine Leaf Stripe Disease, Wood Integrity, and the Mycobiome in Grapevine Trunks.

Previous works on grapevine-trunk diseases indicate that minimal or non-pruning of the grapevine under certain circumstances can significantly reduce the risk of symptom expression. Nevertheless, knowledge of the mechanisms behind these observations are limited. Therefore, it was the aim of this study to investigate in more detail the effect of pruning intensity on the grapevine trunk by means of trunk integrity and the fungal community in the wood tissue. Two German vineyards partially trained in vertical-shoot position and semi-minimally pruned hedges were chosen for this survey due to the accessibility of multi-annual esca-monitoring data. The results revealed that only in one of the two vineyards was the incidence of external esca symptoms significantly reduced over a period of five years (2017–2021) by minimal pruning, which was up to 73.7% compared to intensive pruning. In both vineyards, the trunks of intensively pruned vines not only had more pruning wounds on the trunk (by 86.0% and 72.9%, respectively) than minimally pruned vines, but also exhibited a larger (by 19.3% and 14.7%, respectively) circumference of the trunk head. In addition, the percentage of white rot and necrosis in the trunks of esca-positive and esca-negative vines was analyzed and compared between the two pruning intensities; hereby, significant differences were only found for esca-negative ‘Dornfelder’ vines, in which the proportion of necrosis was higher for intensively pruned vines (23.0%) than for minimally pruned vines (11.5%). The fungal communities of the differently pruned vine trunks were mainly dominated by four genera, which are also associated with GTDs: Diplodia, Eutypa, Fomitiporia and Phaeomoniella. All in all, the fungal diversity and community composition did not differ between minimally and intensively pruned, esca-positive vines.

Estimation of Leaf Area Index in vineyards by analysing projected shadows using UAV imagery

A few decades ago, farmers could precisely monitor their croplands just by walking over the fields, but this task becomes more difficult as farm size increases. Precision viticulture can help better understand the vineyard and measure some key structural parameters, such as the Leaf Area Index (LAI). Remote Sensing is a typical approach to monitoring vegetation which measures the spectral information directly emitted and reflected from vegetation. This study explores a new method for estimating LAI which measures the projected shadows of plants using UAV (unmanned aerial vehicle) imagery. A flight mission over a vineyard was scheduled in the afternoon (15:30 to 16:00 solar time), which is the optimal time for the projection of vine shadows on the ground. Real LAI was measured destructively by removing all the vegetation from the area. Then, the projected shadows in the image were detected using machine learning methods (k-means and random forest) and analysed at pixel level using a customised R code. A strong linear relationship (R² = 0.76, RMSE = 0.160 m² m-2 and MAE = 0.139 m² m-2) was found between the shaded area and the LAI per vine. This is a quick and simple method, which is non-destructive and gives accurate results; moreover, flights can be scheduled during other periods of the day than solar noon, such as in the morning or afternoon, thus enabling pilots to extend their working day. Therefore, it may be a viable option for determining LAI in vineyards trained on Vertical Shoot Positioned (VSP) systems.

Biochemical profile of ‘Niagara Rosada’ grapes under different rootstocks and training system

Grapevines are used to be trained to different systems, while there is a paucity of literature on the main cordon disposition with regards to grape quality, especially grafted on to different rootstocks. Therefore, a field experiment took place in the city of São Manuel, state of São Paulo, Brazil. This study aimed to evaluate the quality of the ‘Niagara Rosada’ grapes by training them to vertical shoot position (VSP) system with unilateral and bilateral cordons grafted on to rootstocks during two crop seasons (2015 and 2016). To assess the data, a 22 factorial setup organized in a randomized complete block design was used, corresponding to two rootstocks (‘IAC 766 Campinas’ and ‘IAC 572 Jales’) and two cordons (unilateral and bilateral cordon). The variables assessed were the content of soluble solids, titratable acidity, fruit-ripeness index, reducing sugars, total phenolic compounds, total flavonoids, anthocyanins, antioxidant activity via DPPH and FRAP. Results indicated that ‘IAC 766 Campinas’ rootstock and bilateral cordon improved the chemical quality in grapes, especially the contents of anthocyanins and sugars. This management practice should be selected when growing ‘Niágara Rosada’ grapes, provided that there is an influence in the quality of the final product.

‘Frontenac’ Grape Response to Canopy Management in North Dakota

Experiments were conducted in 2013 and 2014 to evaluate the effects of training system and leaf removal treatments on yield and quality for ‘Frontenac’, an interspecific hybrid wine grape, at a research vineyard located near Absaraka, North Dakota. The experiment was structured as a randomized complete block design with a split-plot arrangement including four training system treatments (Geneva Double Curtain (GDC), High Cordon (HC), Vertical Shoot Positioned (VSP), Four-Arm Kniffin (4AK)), and four-leaf removal timing treatments (bloom, post-bloom, veraison, and a control, no removal) with eight replicates. In 2013, 1428 growing degree days (GDDs) accumulated in the 155 days between frost events. In 2014, 1156 GDDs accumulated in the 121 days between frost events, 272 GDDs less than the year prior. Even with the large GDD differences between years, there was no significant interaction between trellis type and leaf removal, and the main factor of leaf removal did not influence any of the fruit variables where data were collected. Combined data analysis showed no significant differences in fruit juice total soluble solids (TSS) or titratable acidity (TA). The fruit juice pH in 2013 was greater when grapes were grown in the VSP system compared to grapes grown in the other trellis systems. In 2014, live nodes and total shoots were greater for grapes grown on GDC and 4AK systems compared to grapes grown on the HC and VSP systems. Additionally, in 2014, grapes grown on the GDC system had a greater cluster number and yield when compared to grapes grown on the 4AK or VSP systems. These findings suggest that ‘Frontenac’ fruit TSS accumulation and TA were not affected by leaf removal or trellis system in North Dakota vineyards and that yield gains may be reached due to the training system without negatively affecting fruit quality.

Modeling Carbon Balance and Sugar Content of Vitis vinifera under Two Different Trellis Systems.

Environmental factors might influence the carbon balance and sugar content in grapevine. In this two-year research, the STELLA software was employed to predict dry matter accumulation in Sangiovese vines, comparing the traditional vertical shoot positioning (VSP) and the single high wire (SHW) trellis systems. Every week, vegetative, eco-physiological and grape quality parameters were collected for 15 tagged vines per trellis system to set up the software. Significant differences in photosynthesis were recorded in 2014, with higher values in VSP (23–25% more). Shoot growth was significantly higher in VSP (20–25% more), whereas higher dry matter (30%) and yield (9–11% more) were detected for SHW. At harvest, berry composition suggested a slower ripening in SHW compared to VSP, which was linked to the shading of clusters in SHW. Finally, for the first time, linear regressions were found between measured berry sugar content and STELLA-estimated dry matter (R2 = 0.96 in VSP; R2 = 0.95 in SHW). This latter evidence allowed the estimation of berry sugar content, showing this software to be a practical tool to support winegrowers in decision making. Other studies are already underway to calibrate and validate the model for other varieties, training systems and environments.