Over recent decades, numerous strong earthquakes have caused widespread devastation, including citywide destruction, significant loss of life, and severe structural damage. Seismic base isolation is a well-established method for mitigating earthquake-induced risks in buildings; however, its high cost often limits its implementation in developing countries. Simultaneously, the global rise in vehicle numbers has led to the accumulation of discarded tires, intensifying environmental challenges. In response to these issues, this study investigates the development of a seismic isolator matrix using recycled rubber from vehicle tires, proposed as a sustainable and cost-effective alternative. Ten recycled rubber matrices were experimentally evaluated for their physical and mechanical properties. The matrix with optimal granulometry and binder content, demonstrating superior performance, was identified. This optimized matrix underwent further validation through compression and cyclic shear tests on reduced-scale prototypes of fiber-reinforced isolators, which included five prototype designs, two of which featured flexible reinforcement. The best-performing prototype comprised a recycled rubber matrix with 15% binder and glass fiber, exhibiting vertical stiffness and damping characteristics superior to those of natural rubber. Specifically, this prototype achieved a damping ratio of up to 22%, surpassing the 10% minimum required for seismic isolation, along with a vertical stiffness of 45 kN/mm, critical for withstanding the vertical loads transferred by buildings. These findings suggest that the recycled tire rubber matrix, when combined with glass fiber, is a viable material for the production of seismic isolators. This combination utilizes discarded materials, contributing to environmental sustainability.