Rape Varieties Research Articles

Methyl jasmonate-induced bHLH42 mediates tissue-specific accumulation of anthocyanins via regulating flavonoid metabolism-related pathways in Caitai.

Anthocyanin is a type of plant secondary metabolite beneficial to human health. The anthocyanin content of vegetable and fruit crops signifies their nutritional quality. However, the molecular mechanism of anthocyanin accumulation, especially tissue-specific accumulation, in Caitai, as well as in other Brassica rapa varieties, remains elusive. In the present study, taking advantage of three kinds of Caitai cultivars with diverse colour traits between leaves and stems, we conducted a comparative transcriptome analysis and identified the molecular pathway of anthocyanin biosynthesis in Caitai leaves and stems, respectively. Our further investigations demonstrate that bHLH42, which is robustly induced by MeJA, closely correlates with tissue-specific accumulation of anthocyanins in Caitai; bHLH42 upregulates the expression of flavonoid/anthocyanin biosynthetic pathway genes to activate anthocyanin biosynthesis pathway, importantly, overexpression of bHLH42 significantly improves the anthocyanin content of Caitai. Our analysis convincingly suggests that bHLH42 induced by jasmonic acid signalling plays a crucial role in tissue-specific accumulation of anthocyanins in Caitai.

Screening of low-Cd-accumulating and Cd-remediating oilseed rape varieties using a newly indicator system for risk management of Cd-contaminated agricultural land

Although oilseed rape is frequently used as an alternative planting crop in the phytoremediation of cadmium (Cd)-contaminated agricultural land, methods for screening excellent oilseed rape varieties in this regard are inadequate. Herein, we developed a screening method that incorporates Cd accumulation, distribution, and removal, economic output, adaptability to Cd-contaminated agricultural land, and trace element variation. A Cd-adaptability index (Cd-AI) based on 10 agronomic traits was used to measure the adaptability of varieties to Cd-contaminated agricultural land. Moreover, to simplify the evaluation of adaptability, yield, biomass, and pod number with high weightings were selected to construct a discriminant function for Cd-contaminated agricultural land adaptability (correctly classified 94.20%). In a 2 year field trial, we evaluated 225 oilseed rape varieties, among which we identified two promising low-Cd-accumulating and two Cd-remediating varieties. For the low-Cd-accumulating varieties (HuYou17 and DeXingYou558), we obtained grain bioaccumulation factor (BAF) values of 0.07 and 0.08, BAFsoil-stalk values of <1, and economic outputs of RMB 25,054 and 32,292 yuan hm−2, respectively. Similarly, the Cd-remediating varieties (ZaoZa8 and YuYou61) were characterized by BAFsoil-stalk values of 4.65 and 3.61, BAFsoil-grain values of 0.16 and 0.16, Cd removals of 69.02 and 58.25 g hm−2, and economic outputs of RMB 31,189 and 24,962 yuan hm−2, respectively. Compared with the control variety, we detected lower uptakes of multiple trace elements (3–43%) in the low-Cd-accumulating varieties, whereas the Cd-remediating varieties were characterized by 15.40% and 8.30% increases in the accumulation of magnesium and zinc, respectively. Our findings augment the evaluation indices used for evaluating oilseed rape varieties and provide valuable insights from the perspectives of varietal screening and promotional application. The effective varieties identified have application potential for safe production and the remediation of agricultural land without interrupting annual agricultural production, and provide an economically sustainable approach for the utilization of Cd-contaminated agricultural land.

A new spring rape variety for Siberia conditions

A new spring rape variety for Siberia conditions

Economic and biological value of spring rape varieties for solving the protein problem under the conditions of the Southern Trans-Urals region

Rapeseed is an excellent representative of forage crops, which has no equal in nutritional value, feeding value or low cost of production. It can be considered an excellent source of edible oil, as well as feed protein, and occupies one of the leading positions in solving problems associated with the availability of protein nutrition in animals. The importance of this crop has increased with the development of various modern erucosinolate-free and low-glucosinolate varieties. The purpose of the research was to study the possibility of obtaining a stable yield of oilseeds and herbage mass of spring rape, identifying the most productive varieties for use in solving the problem of protein in feed under the conditions of the Southern Trans-Urals. Field experiments were carried out at the Kurgan State Agricultural Academy named after T. S. Maltsev – branch of the Federal State Budgetary Educational Institution of Higher Education “Kurgan State University” in 2021–2023. During the years of research, a negative eff ect of drought on the yield of seeds and green mass of spring rapeseed varieties was noted. The lowest average seed yield was obtained for all tested varieties in 2022 – 1,99 t/ha, in 2023 the yield was 2,23 t/ha and in 2021 – 2,37 t/ha. The strength of influence of the “weather conditions of the year” factor was 24,5 %, and the “variety” factor on the productivity of spring rapeseed was 35,7 %, which was by 1,5 times higher than the “weather conditions of the year” factor. The highest herbage mass yield on average was obtained on the variety Yarilo and amounted to 20,6 t/ha, then followed by the variety Yubileiny (standard). The lowest average over three years of research was shown by the variety Ratnik – 9,6 t/ha.

ACBP4-WRKY70-RAP2.12 module positively regulates submergence-induced hypoxia response in Arabidopsis thaliana.

ACYL-CoA-BINDING PROTEINs (ACBPs) play crucial regulatory roles during plant response to hypoxia, but their molecular mechanisms remain poorly understood. Our study reveals that ACBP4 serves as a positive regulator of the plant hypoxia response by interacting with WRKY70, influencing its nucleocytoplasmic shuttling in Arabidopsis thaliana. Furthermore, we demonstrate the direct binding of WRKY70 to the ACBP4 promoter, resulting in its upregulation and suggesting a positive feedback loop. Additionally, we pinpointed a phosphorylation site at Ser638 of ACBP4, which enhances submergence tolerance, potentially by facilitating WRKY70's nuclear shuttling. Surprisingly, a natural variation in this phosphorylation site of ACBP4 allowed A. thaliana to adapt to humid conditions during its historical demographic expansion. We further observed that both phosphorylated ACBP4 and oleoyl-CoA can impede the interaction between ACBP4 and WRKY70, thus promoting WRKY70's nuclear translocation. Finally, we found that the overexpression of orthologous BnaC5.ACBP4 and BnaA7.WRKY70 in Brassica napus increases submergence tolerance, indicating their functional similarity across genera. In summary, our research not only sheds light on the functional significance of the ACBP4 gene in hypoxia response, but also underscores its potential utility in breeding flooding-tolerant oilseed rape varieties.

РОЛЬ ФУНГИЦИДОВ В ПОВЫШЕНИИ ПРОДУКТИВНОСТИ ОЗИМЫХ СУРЕПИЦЫ И РАПСА

The paper analyses long-term studies into the effectiveness of fungicidal treatment of winter turnip rape and winter rapeseed with preparations PiktorAktiv, Karamba Duo. The studies were conducted on dark gray soils of Ryazan region; the experiment was set up in 2018–2022. The experiment revealed that variants treated with the Piktor Aktiv and Karamba Duo preparations had high survival of plants for harvesting. On average, over the experimental period, variants Severyanin + PiktorAktiv, Karamba Duo 70.1 % (87.6 pcs/m2 ), and F1 Mercedes + PiktorAktiv, Karamba Duo 68.4 % (85,5 pieces/m2 ) had the maximum survival rate of rapeseed plants. The effective action of fungicides PiktorAktiv and Karamba Duo was also traced with turnip rape variants: the survival rate was 51.9 % (103.8 pcs/m2 ) in variety Enigma, it was 49.1 % (98.2 pcs/m2 ) in variety Zarya. On average, the study results demonstrated the maximum gain in the variant of winter turnip rape treated with fungicides PiktorAktiv, Karamba Duo: the F1 Mercedes hybrid had 26.7 c/ha (+3.7 c/ha, or +16.0 %); the Severyanin variety had 26.6 centners/ha (+3.6 centners/ha, or +15.6 %). The studies proved the efficacy of fungicidal preparations in agrocenoses of winter oilseed crops of the Brassica family. The research studies showed high productivity of winter turnip rape varieties Enigma and Zarya, winter rapeseed variety Severyanin and hybrid Mercedes grown in the conditions of the southern part of the Non-Black soil zone of Russia.

Inter-Varietal Variation in Phenolic Profile, Antioxidant, Anti-Inflammatory and Analgesic Activities of Two Brassica rapa Varieties: Influence on Pro-Inflammatory Mediators.

The present research study aims to appraise the potential of polyphenol-rich extracts from two Brassica rapa varieties on antioxidant, anti-inflammatory and analgesic activities using carrageenan-induced paw edema model in rats. Methanol extracts of peels and pulps of Brassica rapa yellow root (BRYR) and Brassica rapa white root (BRWR) were prepared using the soxhlet extraction technique. All four extracts were analyzed by reversed-phase high-pressure liquid chromatography (RP-HPLC) for the polyphenols, and results showed that 10 phenolic acids and 4 flavonoids were detected. Gallic acid was the major phenolic acid (174.6-642.3 mg/100 g of dry plant material) while catechin was the major (34.45-358.5 mg/100 g of dry plant material) flavonoid detected in the extracts. The total phenolic contents (TPC) of BRYR peel, BRWR peel, BRYR pulp and BRWR pulp extracts were in the range of 1.21-5.01 mg/g of dry plant material, measured as GAE, whereas the total flavonoid contents (TFC) were found in the range of 0.90-3.95 mg/g of dry plant material, measured as QE. BRYR peel extract exhibited the best DPPH radical scavenging activity (IC50, 3.85 µg/mL) and reducing potential as compared with other extracts. The in vivo anti-inflammatory potential was assessed by carrageenan-induced rat paw edema, and the analgesic potential was investigated by a hot plate test. Suppression of biochemical inflammatory biomarkers including C-reactive protein (CRP), rheumatoid factor (RF) and tumor necrosis factor (TNF-α), and interleukin-6 (IL-6) concentration were also determined. Results showed that BRYR peel extracts reduced paw edema and suppressed the production of TNF-α, IL-6, CRP and RF most significantly, followed by BRWR peel, BRYR pulp and BRWR pulp extracts. In addition, histopathology observation also supports the anti-inflammatory effect of peel extracts as being greater than that of root pulp extracts. Moreover, it was observed that the analgesic effect of the root-peel extracts was also more pronounced as compared with root-pulp extracts. It can be concluded that BRYR peel extract has higher phenolic contents and showed higher suppression of TNF-α, IL-6, CRP and RF, with strong antioxidant, anti-inflammatory and analgesic effects.

Combined archaeobotanical and linguistic evidence does not support the early domestication of Brassica rapa varieties

Combined archaeobotanical and linguistic evidence does not support the early domestication of Brassica rapa varieties

Effect of different concentrations of pyridoxine on physiological indices of Brassica rapa varieties

Effect of different concentrations of pyridoxine on physiological indices of Brassica rapa varieties

Economic and biological evaluation of spring rape varieties and hybrids under conditions of the Kirov region

In recent years there has been a tendency in the Russian Federation to increase the area under rapeseed, the area of which is constantly expanding. Research on economic and biological evaluation of spring rape varieties and hybrids was carried out in one of the farms of the Kirov region. Promising varieties and hybrids were studied under comparative aspect in production conditions. The seeds field germination was studied, the safety of the plants by the time of harvesting was determined. The duration of interphase and vegetation periods of the promising varieties and hybrids of spring rape was estimated, the yield of their seeds was compared. Analysis of the productivity structure elements of these varieties and hybrids is presented. In the experiment spring rape varieties Heros, Campino, Yarilo, obtained under farm conditions and having appropriate quality documents, and the first generation hybrids Joy, Jazz, Hephaestus, Joker, Jerome, Jerry were sown. When assessing the duration of the vegetation period, it was found that all the samples by biological rhythms fit into the vegetation period of the Kirov region and belong to the medium-maturing group. Field germination of the studied varieties and hybrids on average during the years of the study ranged from 48.8 to 75.8%. On average for 2 years, the highest field germination was observed in the first generation hybrids Joker and Hephaestus (75.8 and 69.1%, respectively). Preservation of plants for harvesting higher than the control (84%), on average for 2 years was observed in the varieties Campino and Yarilo. In terms of seed yield, hybrids Jerry F1 (16.7 c/ha) and Joker F1 (16.2) and the variety Yarilo (16.3 c/ha) significantly outperformed the control (15.5 c/ha). Hybrids Joy (450), Joker (482) and the variety Yarilo (459) reliably surpassed the control (358) by the number of pods on one plant on average for 2 years.

Mulching with oilseed rape improving soil moisture and fertility and apple yield in orchard in semi‐arid region

AbstractThis study was aimed to alleviate water deficiency and improve soil fertility in orchards in a semi‐arid region. The Brassica napus L. winter varieties (including the varieties V6 and V7) (V6 and V7 belong to the Brassica napus L. winter variety 1721‐1B, and Qin‐you‐7) generally had higher aboveground biological yield, lower root yield, and main root length compared to the Brassica campestris L. winter or spring varieties. By mulching root area of apple tree with aboveground part of these rape varieties in later autumn, the water contents in 0–20, 20–40, and 40–60 cm soil were enhanced or changed at following March and positively correlated with the mulching‐plant biomass. Similarly, under treatments of the V6‐I and V7‐I (V6‐I and V7‐I belong to mulching root area of apple tree with aboveground part of the variety V6 and V7) (the variety V6 and V7 mulching), and the V6‐II and V7‐II (V6‐II and V7‐II belong to mulching root area of apple tree with aboveground part of the V6 and V7 + 5% soil), the average water content in 0–150 cm soil was significantly increased by about 12%, 8%, 18%, and 15% at following spring, respectively; meanwhile, the treatment V6‐II and V7‐II induced significant increase in water contents in 0–25, 25–50, 50–75, 75–100, 100–125, and 125–150 cm soil profile; these treatments were conducted over 2 years; the available K, total N, and organic matter in 0–20 cm soil was significantly increased by 39.3%–151.8%, 10.0%–17.9%, and 22.7%–34.2%, the apple tree growth was promoted, and the fruit yield per plant was significantly enhanced by 16.9%–95.5%. In conclusion, the Brassica napus L. winter variety mulching, especially the mulching with the aboveground part of the Brassica napus L. winter variety + 5% soil, showed significant effect in improving soil moisture, soil fertility, and fruit yield in orchards in semi‐arid regions.

Profiling bacterial communities of irrigation water and leafy green vegetables produced by small-scale farms and sold in informal settlements in South Africa

Morogo is an African indigenous term used for leafy green vegetables harvested in the wild or cultivated in small-scale farms and consumed by the local populations of the region. Small-scale farmers have gained recognition as important suppliers of morogo to informal settlements. In commercial production systems, leafy green vegetables have increasingly been reported as associated with foodborne pathogens and disease outbreaks. Little is known of the presence of these organisms on leafy green vegetables in the informal unregulated food systems. This study aimed to profile bacterial communities in irrigation water (flooding and overhead irrigation water) and leafy green vegetables (Brassica rapa L. chinensis and Brassica rapa varieties of morogo) to establish the natural bacterial flora at the water-fresh produce interface from five small-scale farms in two provinces in South Africa. Illumina MiSeq high-throughput sequencing showed that each farm exhibited a unique bacterial community composition, with an overall high relative abundance of Proteobacteria, Firmicutes and Actinobacteria, including prominent families such as Burkholderiaceae (48%), Enterobacteriaceae (34%), Bacillales Family XII (8%), Rhodobacteraceae (3%), Micrococcaceae (1.98%) and Pseudomonadaceae (1.79%). Specific Enterobacteriaceae Serratia, Enterobacter, Salmonella, Shigella, Escherichia coli, Buchnera, Citrobacter, Klebsiella and Proteus were identified, in addition to unique communities associated with plant or irrigation water source. These findings suggest that the edible plant microbiome can play an important role as transient contributor to the human gut and has the potential to affect overall health.

A review of sources of resistance to turnip yellows virus (TuYV) in Brassica species.

Turnip yellows virus (TuYV; previously known as beet western yellows virus) causes major diseases of Brassica species worldwide resulting in severe yield-losses in arable and vegetable crops. It has also been shown to reduce the quality of vegetables, particularly cabbage where it causes tip burn. Incidences of 100% have been recorded in commercial crops of winter oilseed rape (Brassica napus) and vegetable crops (particularly Brassica oleracea) in Europe. This review summarises the known sources of resistance to TuYV in B. napus (AACC genome), Brassica rapa (AA genome) and B. oleracea (CC genome). It also proposes names for the quantitative trait loci (QTLs) responsible for the resistances, Turnip Yellows virus Resistance (TuYR), that have been mapped to at least the chromosome level in the different Brassica species. There is currently only one known source of resistance deployed commercially (TuYR1). This resistance is said to have originated in B. rapa and was introgressed into the A genome of oilseed rape via hybridisation with B. oleracea to produce allotetraploid (AACC) plants that were then backcrossed into oilseed rape. It has been utilised in the majority of known TuYV-resistant oilseed rape varieties. This has placed significant selection pressure for resistance-breaking mutations arising in TuYV. Further QTLs for resistance to TuYV (TuYR2-TuYR9) have been mapped in the genomes of B. napus, B. rapa and B. oleracea and are described here. QTLs from the latter two species have been introgressed into allotetraploid plants, providing for the first time, combined resistance from both the A and the C genomes for deployment in oilseed rape. Introgression of these new resistances into commercial oilseed rape and vegetable brassicas can be accelerated using the molecular markers that have been developed. The deployment of these resistances should lessen selection pressure for resistance-breaking isolates of TuYV and thereby prolong the effectiveness of each other and extant resistance.

Comparative Study of Bolting Adaptability between 60Co-Induced Rape and Its Original Material

In southern China, the fresh shoots of rape are used as a high-quality seasonal vegetable owing to their pleasant taste. In this study, we investigated the taste and quality of fresh shoots of Fanmingyoutai, which was derived from WH23 by 60Co mutation. WH23 was used as a control (CK). Physiological indexes, transcriptome analyses, and metabolomics analyses between Fanmingyoutai and CK were studied and the related key differential genes were identified. The results showed that the glucosinolate content of Fanmingyoutai seeds was 51.14% lower than that of CK, and the contents of soluble sugar and vitamin C in the fresh shoots of Fanmingyoutai were 2.1 times and 1.4 times higher, respectively, than CK. Using transcriptome analyses, we identified that the differential genes were involved in glycan biosynthesis and metabolism, energy metabolism, carbohydrate metabolism, and the metabolism of cofactors and vitamins. Metabolomics analyses demonstrated that the contents of sucrose and D-fructose in the fresh shoots of Fanmingyoutai were 1.22 times and 1.15 times higher, respectively, than those in CK. Using qRT-PCR analyses, the expression of SWEET17, STP5, and GSL in the fresh shoots and leaves of Fanmingyoutai was two times higher than that in CK. SWEET17 (involved in sugar production and transport), STP5 (involved in monosaccharide transport), and GSL (involved in glucosinolate accumulation) may be the key functional genes. We concluded that the low glucosinolate content and high sucrose and D-fructose contents may be the main factors affecting the taste of fresh shoots of Fanmingyoutai and CK; SWEET17, STP5, and GSL may be the key related genes. This research provides a reference for the breeding and molecular mechanisms of new edible rape varieties.

Differences in N use efficiency, N translocation and N immobilization capacity of their residues of oilseed rape varieties due to N fertilization

ABSTRACT Winter oilseed rape (OSR) is known to poorly utilize nitrogen (N), thus increasing the risk of N losses. In five environments in Germany, eight OSR varieties were grown in combination with five fertilizer N treatments enabling to fit N response curves for each variety separately. At Hohenschulen, additional plant sampling after flowering and at harvest allowed to calculate N translocation from the vegetative plant parts into the seeds and to estimate the potential of the residues to immobilize N after harvest. Nitrogen fertilization increased seed and N yield. Varieties differed significantly in their yields and consequently in their NUE, however, without any significant interaction with the N supply. Total N accumulation at harvest, (N) harvest index and N translocation after flowering into the seeds as well as the N immobilization potential (Ipot) of the residues followed a similar pattern. Without N, the low amount of residues reduced Ipot despite the wide C/N ratio, while a high N fertilization only slightly increased the amount of residues, but clearly decreased their C/N ratio resulting in a lower Ipot. Our results support the approach that breeding for increased seed yields seems to be the most promising way to also improve NUE.

Comparative Study of the Nutritional and Chemical Composition of New Oil Rape, Safflower and Mustard Seed Varieties Developed and Grown in Serbia.

Oilseed crops are widely cultivated and are related to nutrition and human health as valuable nutraceutical sources with valuable biological properties. The growing demand for oil plants used in human and animal nutrition or for the processing industry has contributed to the diversification and development of a new variety of oil crops. Increased oil crop diversity, besides ensuring reduced sensitivity to pests and climate conditions, has also led to improved nutritional values. In order to enable oil crop cultivation to become commercially sustainable, a comprehensive characterization of newly created varieties of oilseeds, including their nutritional and chemical composition, is required. In this study, two varieties of safflower and white and black mustard were investigated as alternative oil species for nutritional parameters, mainly protein, fat, carbohydrate, moisture, ash, polyphenols, flavonoids, chlorophylls contents, acids and mineral composition, and compared with those of two different genotypes of rapeseeds as a traditional oil crop plant. The proximate analysis found that the highest oil content was found in the oil rape NS Svetlana genotype (33.23%), while the lowest was in black mustard (25.37%). The protein content varies from around 26% in safflower samples to 34.63%, determined in white mustard. High content of unsaturated fatty acids and low content of saturated fatty acid was observed in the analyzed samples. In mineral analysis, the dominant elements were phosphorus, potassium, calcium and magnesium, in descending order. The observed oil crops are also good sources of microelements, including iron, copper, manganese and zinc, accompanied by high antioxidant activity due to the presence of significant amounts of polyphenolic and flavonoid compounds.

Identification and Expression Analysis of the Isopentenyl Transferase (IPT) Gene Family under Lack of Nitrogen Stress in Oilseed (Brassica napus L.).

BnIPT gene family members in Brassica napus and analyzing their expression under different exogenous hormones and abiotic stress treatments to provide a theoretical basis for clarifying their functions and molecular genetic mechanisms in nitrogen deficiency stress tolerance of B. napus. Using the Arabidopsis IPT protein as the seed sequence, combined with the IPT protein domain PF01715, 26 members of the BnIPT gene family were identified from the whole genome of the rape variety ZS11. Additionally, the physicochemical properties and structures, phylogenetic relationships, synteny relationships, protein-protein interaction network, and gene ontology enrichment were analyzed. Based on transcriptome data, the expression patterns of the BnIPT gene under different exogenous hormone and abiotic stress treatments were analyzed. We used the qPCR method to identify the relative expression level of BnIPT genes that may be related to the stress resistance of rapeseed in transcriptome analysis under normal nitrogen (N: 6 mmol·L-1) and nitrogen deficiency (N: 0) conditions and analyzed its effect on rapeseed under nitrogen deficiency stress role in tolerance. In response to nitrogen deficiency signals, the BnIPT gene showed a trend of up-regulation in shoots and down-regulation in roots, indicating that it may affect the process of nitrogen transport and redistribution to enhance the stress resistance of rapeseed to respond to the nitrogen deficiency stress. This study provides a theoretical basis for clarifying the function and molecular genetic mechanism of the BnIPT gene family in nitrogen deficiency stress tolerance in rape.

Identification of Heat-Resistant Varieties of Non-Headed Chinese Cabbage and Discovery of Heat-Resistant Physiological Mechanisms

Affected by global warming, continuous high temperature has a negative impact on plant growth and development and become a major constraint to crop production. Germplasm resource identification has become a research hotspot in many fields, and it is also necessary to establish effective identification methods. In this study, twenty Brassica rapa varieties were selected to investigate the physiological and biochemical characteristics under heat stress, explore the relationship between physiological response and the heat resistance mechanism, and select some typical heat-resistant and heat-sensitive varieties. The effects of photosynthetic electron transfer and antioxidant pathway on the heat resistance of Brassica rapa were identified. These findings will provide important guidance for the physiological regulation and identification method of heat stress in plants.

OIL CONTENT IN WINTER RAPE SEEDS DEPENDING ON MICRONUTRIENT NUTRITION

The article is devoted to the study of the role of micronutrients in their application in the form of foliar fertilization in view of their influence on the quality indicators of the formed crop, in particular on the oil content of seeds as a basic indicator that determines the value of winter rape as a crop for multipurpose use. The effectiveness of monocomponent application of the main physiologically active microelements in two phenological critical phases of growth and flowering of winter rape plants with different dominance of growth and growth-reproductive processes of plant organism differentiation - in the phenological phases of stemming and flowering - was investigated. The effectiveness of the influence of single-component microelements selected by the typological composition of modern multicomponent chelated microfertilizers in the form of sulfate salts, boric acid and ammonium sulfate at a physiological concentration of 0.2 %, taking into account the regulation of concentrations of physiologically active agrochemicals in the format of foliar feeding with aqueous salt solutions, was determined. It was found that the use of each of the studied microelements was effective and significant in terms of increasing the oil content of seeds in comparison with the control variant of water treatment. Individual peculiarities of seed oil content formation for two high-intensity varieties of winter rape for each of the studied microelements were established both from the standpoint of the increase in oil content to the control and from the standpoint of assessing the variability of its value, taking into account the two-year cycle of study and variation within the general set of the obtained accounting values within the replications of the experiment. On the basis of the data obtained, the expediency of the timing of foliar fertilization with microfertilizers of winter rape hybrids was determined and the role of the genotypic component in the effectiveness of the use of corrective foliar fertilization with various microelements was noted. It was established on the basis of a two-year research cycle for a guaranteed increase of oil content in seeds of intensive winter rape hybrids by at least 0.6-1, 8 % under the condition of one-component use of trace elements in the form of foliar feeding, they should be used in the following order of increasing expediency and validity of Co&gt;Zn&gt;B&gt;Cu&gt;Mn&gt;Mo in the form of a solution of salt sulfates, boric acid and ammonium molybdate at 0.2 % concentration of the working solution for foliar feeding. Prospects for further research in the field of optimization of fertilization of intensive hybrids of winter rape with microfertilizers are formulated.

Statistical Multivariate Methods for the Selection of High-Yielding Rapeseed Lines with Varied Seed Coat Color

A crucial aim for rapeseed breeders is increasing the seed yield through improving yield- and silique-related traits. In this research, traits including the number of seeds per silique, silique length, silique width, and thousand-seed weight combined with seed coat color were investigated. Yellow-seeded lines of oilseed rape have a thinner seed coat, lower fiber content, and higher protein and oil content than traditional varieties. Hybrids of yellow- and black-seeded Brassica napus, which combine the advantages of both types of seeds, are tested for their suitability in breeding. Therefore, the aim of this study was to assess a population of 78 doubled haploid lines with different seed coat colors to select genotypes connecting a light color of seeds with good yielding characteristics. Multivariate methods such as analysis of variance, correlation analysis, regression analysis, canonical variate analysis, Mahalanobis distances, and estimation of gene effects were used. The results showed that all tested genotypes were significantly different regarding all five studied traits. The multidimensional analyses gave an accurate overview of the relationship between the examined features. The color and weight of seeds were considered the most important for breeding rapeseed with better seed properties. Although a negative correlation between these two characteristics occurs quite commonly, it was not present in our plant material, which allowed us to select lines with a-light seed color and high thousand-seed weight, which is a significant achievement. As a result, two doubled haploid lines were chosen as valuable breeding material for creating oilseed rape varieties with improved biochemical characteristics of seeds.