Variety Of Origins Research Articles

BASS. XXXIV. A Catalog of the Nuclear Millimeter-wave Continuum Emission Properties of AGNs Constrained on Scales ≤ 100–200 pc

We present a catalog of the millimeter-wave (mm-wave) continuum properties of 98 nearby (z < 0.05) active galactic nuclei (AGNs) selected from the 70 month Swift/BAT hard-X-ray catalog that have precisely determined X-ray spectral properties and subarcsecond-resolution Atacama Large Millimeter/submillimeter Array Band 6 (211–275 GHz) observations as of 2021 April. Due to the hard-X-ray (>10 keV) selection, the sample is nearly unbiased for obscured systems at least up to Compton-thick-level obscuration, and provides the largest number of AGNs with high-physical-resolution mm-wave data (≲100–200 pc). Our catalog reports emission peak coordinates, spectral indices, and peak fluxes and luminosities at 1.3 mm (230 GHz). Additionally, high-resolution mm-wave images are provided. Using the images and creating radial surface brightness profiles of mm-wave emission, we identify emission extending from the central sources and isolated blob-like emission. Flags indicating the presence of these emission features are tabulated. Among 90 AGNs with significant detections of nuclear emission, 37 AGNs (≈41%) appear to have both or one of extended or blob-like components. We, in particular, investigate AGNs that show well-resolved mm-wave components and find that these seem to have a variety of origins (i.e., a jet, radio lobes, a secondary AGN, stellar clusters, a narrow-line region, galaxy disk, active star formation regions, or AGN-driven outflows), and some components have currently unclear origins.

Human Papillomavirus (HPV) Infections Among Participants Undergoing Chlamydia trachomatis Testing in Reunion Island (RUN-SurV-HPV Study): Protocol for a Prevalence Study.

Infection by human papillomavirus (HPV) induces various cancers, mainly cervical cancer but also anal and pharyngeal cancers. Reunion Island is a French department in the Indian Ocean. Due to the history of its settlement, the island's population has a wide variety of origins, and the population's sociodemographic characteristics differ from those of the French mainland. These characteristics make the island's population an original French population in its own right, particularly in terms of health. Cervical cancer incidence and mortality in Reunion Island are 10.4 per 100,000 and 4.4 per 100,000, respectively, both of which are much higher than those in mainland France. There is also a disparity in the prevalence of different HPV types, with types 33 and 52 being overrepresented and type 18 being underrepresented. However, vaccination and screening coverage in Reunion Island is low. It is important to understand the burden of infection and its risk factors in members of the young Reunionese population at risk of HPV infection to promote and evaluate the implementation of future vaccination and screening programs on a larger scale. The RUN-SurV-HPV study will have the following four objectives: (1) to describe the prevalence of HPV genotypes in a population at risk of sexually transmitted infections in Reunion Island; (2) to describe the prevalence of HPV genotypes by anatomical site, gender, and sexuality; (3) to describe the correlates and risk factors for HPV detection; and (4) to examine HPV genotypes between different anatomical sites. Cross-sectional analyses of 1200 routine vaginal, anal, pharyngeal, and urinary samples collected between October 2020 to December 2022 from female and male patients aged 16 to 30 years undergoing Chlamydia trachomatis testing at a sexually transmitted infection (STI) testing center at Reunion Island will be performed. The population included 333 women who all had vaginal and pharyngeal swabs, with 80 of them also having had an anal swab. There are 167 men who have sex with men who have had anal and pharyngeal swabs, and 120 men who have sex with women who have had a urine swab only. Results will be correlated with sociodemographic and clinical data collected routinely during the consultations. HPV detection and genotyping will be performed using the Anyplex II HPV28 detection assay (Seegene). The first polymerase chain reactions should begin in November 2023. The first results should be submitted for publication in 2024. The study will determine HPV prevalence by age, sex, male sexual preference, human immunodeficiency virus status, and STI co-infection. Collecting data from young patients not usually routinely screened for HPV infection will be a simple and reproducible way of better understanding local specificities, encouraging vaccination campaigns in the short-term, and evaluating their effectiveness in the future. DERR1-10.2196/47379.

Epigenetic mechanism of Gtl2-miRNAs causes the primitive sheep characteristics found in purebred Merino sheep

BackgroundIt is not uncommon for some individuals to retain certain primitive characteristics even after domestication or long-term intensive selection. Wild ancestors or original varieties of animals typically possess strong adaptability to environmental preservation, a trait that is often lacking in highly artificially selected populations. In the case of the Merino population, a world-renowned fine wool sheep breed, a phenotype with primitive coarse wool characteristic has re-emerged. It is currently unclear whether this characteristic is detrimental to the production of fine wool or whether it is linked to the adaptability of sheep. The underlying genetic/epigenetic mechanisms behind this trait are also poorly understood.ResultsThis study identified lambs with an ancestral-like coarse (ALC) wool type that emerged during the purebred breeding of Merino fine wool sheep. The presence of this primitive sheep characteristic resulted in better environmental adaptability in lambs, as well as improved fine wool yield in adulthood. Reciprocal cross experiments revealed that the ALC phenotype exhibited maternal genetic characteristics. Transcriptomic SNP analysis indicated that the ALC phenotype was localized to the imprinted Gtl2-miRNAs locus, and a significant correlation was found between the ALC wool type and a newly identified short Interstitial Telomeric Sequences (s-ITSs) at this locus. We further confirmed that a novel 38-nt small RNA transcribed from these s-ITSs, in combination with the previously reported 22-nt small RNAs cluster from the Gtl2-miRNAs locus, synergistically inhibited PI3K/AKT/Metabolic/Oxidative stress and subsequent apoptotic pathways in wool follicle stem cells, resulting in the ALC wool type. The necessity of Gtl2-miRNAs in controlling primary hair follicle morphogenesis, as well as the wool follicle type for ALC wool lambs, was verified using intergenic differentially methylated region-knockout mice.ConclusionThe ALC wool type of Merino sheep, which does not reduce wool quality but increases yield and adaptability, is regulated by epigenetic mechanisms in the imprinted Gtl2-miRNAs region on sheep chromosome 18, with the maternally expressed imprinted gene responsible for the ALC phenotype. This study highlights the significance of epigenetic regulation during embryonic and juvenile stages and emphasizes the advantages of early adaptation breeding for maternal parents in enhancing the overall performance of their offspring.

Comprehensive evaluation of introduced cherry varieties cultivated in the submontane province of Dagestan

The study was conducted from 2019 to 2022 with sour cherry varieties cultivated in the conditions of the mountainous province of Dagestan. The research objects were 13 introduced acidic cherry varieties of different ecological and geographical origins. Productivity and assessing the fruit quality parameters were studied using standard methods of variety research and biochemical analysis. It was determined that the varieties Shaten Morel, Heyman Konservn, Vladimirskaia, and Basler Adler are characterised by the highest content of soluble dry matter (14.66–16.21%) and sugars (10.15–11.30%) in the fruits. The varieties Krasa Severa, Basler Adler, Shaten Morel, and Liubskaia proved to be the most valuable in terms of the mass concentration of vitamin C (8.95–10.43 mg%). Liubskaia, Shaten Morel, and Padus Maaki had a higher acid content than the reference variety Podbelskaia (1.24%). Varieties Krasa Severa, Chudo-vishnia, Heyman Konservn, and Basler Adler exhibited large fruit sizes (4.8–6.9 g). The sensory evaluation of the fruits was high (within the range of 4.8–5.0 points on a five-point scale) for the varieties Chudo-vishnia, Heyman Konservn, and Basler Adler. These same varieties demonstrated consistently high yields (5.21–7.66 t/ha) over the years of the study. The results of the comprehensive analysis of the cherry assortment allowed the identification of varieties that are most promising for cultivation on an industrial scale in the territory of Dagestan and use in breeding – Chudo-vishnia, Heyman Konservn, Basler Adler, Krasa Severa, and Shaten Morel.

Phenological phases of &lt;i&gt;Weigela×wagneri&lt;/i&gt; L. H. Bailey under conditions of humid subtropics in Russia

Weigela varieties of hybrid origin, known as Weigela hortensis (W. hortensis C.A. Mey) or &lt;i&gt;Weigela×wagneri&lt;/i&gt; (W.wagneri L. H. Bailey) are cultivated on the Black Sea coast of Krasnodar Krai. A current range of this beautifully flowering shrub includes more than 150 varieties. However, only 24 grow in the region and are predominately selected in the early XX century and are rarely used in the landscaping of the resort town. Ornamental varieties of Weigela grow mostly in limited use areas (Sochi Arboretum, Subtropical Botanical Garden in Kuban region, Friendship Tree Garden Museum). The study aims at investigating the phenological phases of &lt;i&gt;Weigela×wagneri&lt;/i&gt; L. H. Bailey under humid subtropical conditions of the Black Sea coast, Krasnodar Krai, to be used for the design of gardens with continuous flowering plants, as well as for breeding. The study was conducted in the period from 2013 to 2019 in the Friendship Tree Garden Museum and Subtropical Botanical Garden in Kuban region. The study involved 15 varieties of W.×wagneri. The authors established differences in phenological phases, including the start/end of growing season and flowering, duration of these periods, as well as the sums of effective temperatures required for these phases. The start of the growing season was established to vary from February 12 (Arlequin) with an accumulated sum of effective temperature of 261.2℃ to March 14 (Brictol Ruby) and a sum of temperature of 376.9℃. Differences between varieties in this phenophase account for 30 days. On the basis of the long-term average annual data, the varieties were divided into groups according to the floral initiation: early flowering (from April 14 to April 21) group included Arlequin, Gustave Malet, Mon Blanc, Newport Red, Venusta, Madame Lemoine; medium-flowering (April 22 to April 30) group included Lacepede, Ballet, Pavillon Blanc, Brictol Ruby, Madame Lemoine; Eva Ratke, Kosteriana variegata, Red Prince, Augusta were included into a late-flowering (from May 01 to May 06) group. According to the dates of the start and end of growing season, the varieties were divided into the following phenological groups: EE – Early starting (before Feb 12) and Early ending vegetation (before Dec 05); EL – Early starting (before Feb 12) and Late ending (after Dec 19); LE – Late starting (after March 4) and Early ending (before Dec 5); LL – Late starting (after March 4) and Late ending vegetation (after Dec 19).

Comparison of Policosanol Profiles of the Sprouts of Wheat Mutant Lines and the Effect of Differential LED Lights on Selected Lines.

Policosanols (PCs) are long-chain linear aliphatic alcohols that are present in the primary leaves of cereal crops, such as barley and wheat, sugar cane wax, and beeswax. PCs have been used as a nutraceutical for improving hyperlipidemia and hypercholesterolemia. However, the PC content in mutant wheat lines has not been investigated. To select highly functional wheat sprouts with a high content of PCs in wheat mutant lines developed via gamma-irradiated mutation breeding, we cultivated the sprouts of wheat mutant lines in a growth chamber with white LED light (6000 K) and analyzed the PC content in these samples using GC-MS. We studied the PC content in 91 wheat sprout samples: the original variety (Woori-mil × D-7; WS01), commercially available cv. Geumgang (WS87) and cv. Cheongwoo (WS91), and mutant lines (WS02-WS86 and WS88-WS90) developed from WS01 and WS87. Compared to WS01, 18 mutant lines exhibited a high total PC content (506.08-873.24 mg/100 g dry weight). Among them, the top 10 mutant lines were evaluated for their PC production after cultivating under blue (440 nm), green (520 nm), and red (660 nm) LED light irradiation; however, these colored LED lights reduced the total PC production by 35.8-49.7%, suggesting that the cultivation with white LED lights was more efficient in promoting PCs' yield, compared to different LED lights. Therefore, our findings show the potential of radiation-bred wheat varieties as functional foods against hyperlipidemia and obesity and the optimal light conditions for high PC production.

Productivity of soft and hard spring wheat varieties under the conditions of Primorsky Krai

Relevance. Currently, the most important tasks of breeding are the creation and introduction into production of new high—yielding varieties, resistant to stressful environmental conditions and with high grain quality, adapted to the conditions of the monsoon climate.The purpose of the study is to study varieties of spring soft and durum wheat of various origins according to the main economically valuable characteristics in the conditions of the Primorsky Territory.Methods. The research was conducted at FSBSI «FSC of Agricultural Biotechnology of the Far East named after A.K. Chaiki» in 2020–2022. Sixteen spring wheat varieties of various breeding origin served as the research object — six soft spring wheat varieties and ten hard spring wheat varieties. The weather conditions during the growing season of wheat differed over the years of the research (2020–2022). This allowed an objective evaluation of the breeding material for adaptability and resistance to stress factors.Results. For breeding and production, wheat varieties with high yields (more than 4.0 t/ha) have been identified: spring soft — Voronezh 18, Rima, Primorskaya 50, spring hard — Don Elegia. As a result of the correlation analysis, high positive associations were revealed between yield and productive bushiness (r = 0.95), the mass of 1000 grains (r = 0.85), the number of spikelets in the ear (r = 0.84). Aleyskaya, Khabarovsk and Primorskaya 40 varieties stood out with the highest index of protein and gluten in grain. Variety Sunny 573 was distinguished by high hardness (67.5%) and grain type (785 g/l).

The results of the study of multiple selections in a breeding nursery when creating new soybean varieties

Relevance. At the present stage for agricultural production, the creation of new domestic competitive varieties of various plants is of paramount importance within the framework of the large-scale program of the Russian Federation for import substitution, while one of the most important and sought-after crops in Russia is soybean.Methods. The material for the research was the hybrid populations F7-F17 generations of the breeding nursery (SP), which is annually replenished with selections of individual plants from the F6 hybrid nursery.Results. An estimate of the duration of multiple selections by the pedigry method in a breeding nursery (SP) until obtaining homozygous lines is given. The analysis of the conducted studies showed that out of 29 soybean varieties of hybrid origin included in the State Register of Breeding Achievements of the Russian Federation and being tested for 2023, 13 varieties (Harmoniya, Lydia, Nega 1, MK-100, Lebedushka, Zhuravushka, Lacemaker, Cinderella, Tisey, Lyana, Olimp, Lastochka, Tatiana) (44.8%) were obtained on the basis of multiple selections F7-F9 in the breeding nursery, and the variety Persona, which is included in the State Register for 11 regions and is in demand in the Amur Region, was isolated from the hybrid population of the F17 generation. As a result of the assessment for 2020–2022. F7-F17 generations of the breeding nursery according to the duration of splitting in hybrid populations, it was found that multiple selection up to the tenth generation is most effective (F7 — 35.1%, F8 — 30.8%, F9 — 12.1%), but promising highly productive lines with economically valuable traits can also be obtained from later generations

Оценка зимостойкости и засухоустойчивости сортов люцерны изменчивой в условиях Северного Казахстана

Research on the study of the adaptive properties of varieties of alfalfa variable for introduction into the forage production system in the sharply continental conditions of Northern Kazakhstan is an urgent task. The aim of the research is a comparative assessment of alfalfa varieties of different ecological and geographical origin in the conditions of the region. Experimental work was carried out on the basis of stationary field experiments, laid down in the Research and Production Center for Grain Farming named after. A.I. Baraev. The scheme of the experiment, which included 32 varieties of different ecological and geographical origin, including 10 varieties of Kazakhstan selection, 18 varieties of Russian selection, 3 varieties of Canadian selection and 1 variety - Ukrainian. Varieties Raikhan, Shortandinskaya 2, Karaganda 1 Karabalykskaya rainbow, Karabalykskaya Zhemchuzhina, Lyutsiya 14, Lazurnaya of Kazakh selection, varieties Guzel, Muslima, Sarga, Bibinur, Tatar pasture, Uralochka, Nakhodka of Russian selection and varieties Ferax, Rangelander of Canadian selection had an average variability of the trait, about as evidenced by the coefficient of variation V ≤ 20%. .6% drought tolerance. Keywords: ALUCERN VARIABLE, VARIETIES, WINTER HARDER, DROUGH RESISTANCE, VARIATION

Influence of weather conditions on variability of breeding valuable characteristics of winter rye varieties in Kirov region

Long-term results of the research on the influence of weather conditions on the formation of economically biological and technological characteristics of winter rye varieties are valuable for further use in practical breeding, as well as in solving the problem of realizing their biological potential in a wide range of soil and climatic factors. The aim of the research was to evaluate the influence of weather conditions on the variability of yield, economically biological characteristics and baking properties of 10 winter rye varieties of different geographical origin for the subsequent creation of varieties adapted to the conditions of Kirov region. As a result of eight years of research (2015-2022), the variability of 19 biological and technological characters was analyzed in different weather and climatic conditions (index of environmental conditions from +1.44 to -1.39). The highly stable characteristics were grain nature, ear density and relative ear productivity (CV = 1-8 %). The least stable traits were yield and winter hardiness (CV = 75-89 %). Analysis of variability of characteristics by varieties revealed the most adapted to changes in weather conditions varieties - Rushnik, Falenskaya 4, Flora, Alisa (from 2-3 to 38-42 %). The principal component method with visualization on a biplot chart was used to assess the influence of characteristics on yield formation. It was revealed that the yield was more dependent on the weight of 1000 grains, snow mold damage, winter hardiness, resistance to lodging, length and weight of the ear, number of spikelets and grains in the ear, weight of grain per ear and plant, relative productivity of the ear. These traits determined 65.9 % of yield variability. The second biplot chart allowed to identify the most stable varieties for 8 years of study – Rushnik, Falenskaya 4, Flora and Alisa. It was found that the variety of winter rye Alisa realizes its yield potential both in years with optimal conditions of vegetation and in conditions of excessive moisture, but differs from the varieties Falenskaya 4, Flora and Rushnik in high ear productivity and can be used as a source material for breeding.

Genotypical response of barley to increased cadmium content in soil

The results of a comparative evaluation of spring barley (Hordeum vulgare L.) varieties obtained by different methods on control and provocative soil background for cadmium are presented. A total of 10 varieties were studied: Rodnik Prikamya, Novichok, Dina, Zazersky 85, Triumph, Tallon (hybridization and selection); Forward, Bionic, Vitrum (cell selection); In memory of Dudin (mutagenesis). The studies were carried out in 2021 under the climatic conditions of the Kirov region. Seeds of the plants of each variety were sown in vegetation containers filled with soddy-podzolic soil. Cadmium provocative background (6.4 ± 0.5 mg/kg) was created by adding cadmium acetate to the soil. The average yield of barley varieties in the control (soil background without cadmium) was 279 g/m2; against a provocative background – 216 g/m2. Under the conditions of cadmium stress, the yield of hybrids decreased compared to the control by 12.2 %, regenerants – by 29.6 %, mutant – by 42.4 %. Among the studied varieties, the Bionic variety, which has a regenerated origin, showed a consistently high yield both on the control (334 g/m2) and cadmium background (263 g/m2). Productive bushiness of hybrid barley varieties against the control background varied from 2.8 to 4.1; in regenerants – from 3.4 to 4.8; mutant varieties – 4.3 pieces. Under the conditions of cadmium stress, productive bushiness decreased by an average of 2.7 – in hybrids; for 3.4 – for regenerants, for 2.6 pieces – a mutant. Bionic, regardless of the soil background, compared with other varieties, had the highest productive bushiness. Significant differences in the content of polyphenols in the grain of the studied varieties on the control and provocation soil background for cadmium were not revealed. Under the conditions of cadmium stress, a decrease in the content of flavonoids in grain was noted, as well as the accumulation of cadmium (0.29–0.92 mg/kg). Varieties of hybrid origin showed the ability to accumulate cadmium in grain to a greater extent.

Transcriptome analysis reveals the mechanism of different fruit appearance between apricot (Armeniaca vulgaris Lam.) and its seedling.

Apricot fruit has great economic value. In the process of apricot breeding using traditional breeding methods, we obtained a larger seedling (named Us) from the original variety (named U). And Us fruit is larger than U, taste better. Therefore, revealing its mechanism is very important for Apricot breeding. In this study, de novo assembly and transcriptome sequencing (RNA-Seq) was used to screen the differently expressed genes (DEGs) between U and Us at three development stages, including young fruits stage, mid-ripening stage and mature fruit stage. The results showed that there were 6,753 DEGs at different sampling time. "Cellulose synthase (UDP-forming) activity" and "cellulose synthase activity" were the key GO terms enriched in GO, of which CESA and CSL family played a key role. "Photosynthesis-antenna proteins" and "Plant hormone signal transduction" were the candidate pathways and lhca, lhcb, Aux/IAA and SAUR were the main regulators. The auxin signaling pathway was active in Us, of which Aux/IAAs and SAUR were the key fruit size regulators. The low level of lhca and lhcb in Us could reveal the low demand for exogenous carbon, but they increased at mature stage, which might be due to the role of aux, who was keeping the fruit growing. Aux and photosynthesis maight be the main causes of appearance formation of Us fruits. Interestingly, the higher expression of CESA and CSL proved that Us entered the hardening process earlier than U. The advanced developmental progress might also be due to the role of Aux.

DETERMINATION OF LEAF AND FRUIT CHARACTERISTICS OF ALMOND VARIETIES OF FOREIGN ORIGIN CULTIVATED IN CAPPADOCIA REGION

Almond is a type of fruit that grows both economically and naturally in many regions of Turkey. In this study, leaf and fruit characteristics of 3 different almond varieties of foreign origin grown in Ürgüp district of Nevşehir province in the Cappadocia region in 2021 and 2022 were determined. Ferragnes variety with a value of 23.47 mm in terms of leaf width, Ferragnes variety with a value of 67.9 mm in leaf length, and Ferraduel variety with a value of 13.62 in terms of petiole length formed the highest values in the leaf characteristics examined. Considering the fruit data, it was determined that Ferragnes variety had the highest value in terms of fruit length with 40.62 mm, Ferragnes variety had the highest value in fruit width values with 23.99 mm, and Ferragnes variety had the highest value in terms of fruit weight with 6.24 g. When the kernel weight data were examined, the highest value was found in Ferragnes variety with 1.61 g, and when the kernel width and kernel length data were examined, the highest values were determined in Ferragnes variety with 14.55 mm and 27.70 mm, respectively. The results of the study are thought to be important in terms of shedding light on future studies, especially since limited studies have been carried out in this region.

Economically valuable characteristics and breeding indices of spring barley varieties of different ecological and geographical origin in the conditions of the Ryazan region

The article presents the results of research conducted in the collection nursery of spring barley in the conditions in 2020–2022. The purpose of the research is to identify economically valuable traits and calculate breeding indices of selected sources of spring barley of different ecological and geographical origin in the conditions of the forest–steppe zone of the Ryazan region for inclusion in the hybridization program. It was revealed that the varieties Reliable (RF) and Dzivosny (Belarus) had the highest productivity in the conditions of the region with an average yield of 6.48 and 6.40 t/ha, respectively. It was found that the shortest straw had the German varieties KVS Vermont and Ellinor, whose height is in the range of 63-67 cm and varies little over the years of research (CV, % = 3.9 ...6.0). According to the length of the ear, the varieties Bente (Germany) (8.2 cm), Jaromir and Noble (8.0 cm) (RF) stood out. It was revealed that the maximum number of grains in the ear is formed by the Yaromir and Noble varieties – 23.7 pcs. and 23.4 pcs., respectively. According to grain size, the varieties Kufal, Raider (Belarus) and Bente were distinguished, having a mass of 1000 grains of more than 50.0 g. When calculating the breeding indices, it was found that the productivity of domestic varieties was influenced by the Canadian Ki index (r= +0.829) and the harvest index of KHOZ, % (r= +0.559), the Belarusian varieties of the IPPR plant productivity index (r= +0.478), German varieties Ki (r= +0.747) and the linear density index ear of LPC (r = +0.604). On average, according to the number of selected indices, the Noble and Dzivosny varieties come out on top – the maximum indicators for three indices, Yaromir (RF), Ataman (Belarus), KVS Harris, KVS Vermont, Beatrice (Germany) – in second place – for two indices. The varieties of spring barley distinguished by the individual studied positive signs and their complex are recommended for use in breeding programs.

Conservation of the Bird Cherry (Padus Mill.) Germplasm by Cold Storage and Cryopreservation of Winter Cuttings.

Conservation at cryogenic temperatures, usually in liquid nitrogen (LN) or in its vapor, is the only reliable method for the long-term ex situ conservation of fruit and berry crops with vegetative reproduction. In this study, five bird cherry (Padus Mill.) varieties of different genetic origin from the bird cherry genebank at the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR, Russia) were tested for their response to cryopreservation in LN vapor (-183--185 °C). The response included viability under laboratory and field conditions, morphological assessment of the developed plants and biochemical analysis of fruits produced during three consecutive years by plants developed from cryopreserved cuttings. All parameters were compared to those recorded after cold storage of cuttings (-5 °C), a routine mid-term conservation method currently used at the VIR genebank. The initial viability of winter cuttings varied from 86.7% to 93.3%. Six-month cold storage and cryopreservation reduced viability to 53.3-86.7% and 43.3-60.0%, respectively, which was above the 40% viability threshold in all varieties tested. Cuttings after cold storage showed better viability when recovered in the laboratory (80% mean viability) than in the field (58% mean viability); viability of cryopreserved cuttings was not affected by recovery conditions. The results of a two-way analysis of covariance suggested that storage and recovery conditions had the most significant effect on viability (p < 0.0001), while the effects of genotype (p = 0.062) and factor interactions (p = 0.921) were minor. Cryopreservation had little or no influence on morphological parameters of the plants recovered in the field, including plant height, number of shoots, internodes and roots, and root length. Similarly, no effect of cryopreservation was recorded on dry matter content, total sugar content and ascorbic acid concentration in fruits produced by plants developed from the cryopreserved cuttings. These results suggest that cryopreservation in LN vapor is a reliable method for conservation of the bird cherry genetic collection and is worth testing with a broader variety of genotypes.

Assessment of gene pool of black currant Federal Horticultural Center for Breeding, Agrotechnology and Nursery by resistance to currant gall mite in the Bryansk Region

Since controlling currant gall mite (Cecidophyopsis ribis Westw.) is a challenging and ineffective task, developing genetic resistance to the pest is one of the main priorities in breeding programs for Ribes nigrum both in Russia and abroad. The task was assigned at the FSBSO “Federal Horticultural Center for Breeding, Agrotechnology and Nursery” to combine efficiently the positive economic and biological traits of previously developed varieties with resistance to phytophagous pests in hybrid progeny. The study evaluates the genetic collection and hybrid material of black currant for resistance to currant gall mite, as well as identifies the most promising parent pairs for mite-resistant progeny. The research objects included 107 varieties of various genetic origins, 30 of which were of foreign breeding. An assessment of the genetic collection carried out for ten years (since 2012) during the seasons of mass manifestation of the currant gall mite and reversion, is presented. Approximately 1,200 seedlings, planted in 2017, were studied. The methodologies for studying fruit, berry, and nut crops were used to assess the degree of incidence of the currant gall mite. The statistical processing of experimental results was performed using the Microsoft Excel program. As a result, the genetic sources and donors of resistance to the currant gall mite were identified (Barmaley, Dar Smolyaninovoi, Kipiana, Svityazyanka, Chernooka, Wonderful Moment, Ben Hope, Big Ben, etc.). The promising combinations of crosses were identified based on the yield of seedlings resistant to Cecidophyopsis ribis: Ben Hope×Kipiana, Ben Hope×Dar Smolyaninovoi, Kipiana×Dar Smolyaninovoi, Barmalei×Dar Smolyaninovoi, Ben Tirran×Kipiana, 9-28-1/02×Bryansk Agate, Dar Smolyaninovoi×33-27-1, 33-27-1×Kipiana. In the progeny of these parental forms, the selected forms were identified (3-63-01, 3-63-02, 9-197-3, 3-16-1, 7-28-01, 8-123-1), which combine resistance to the currant gall mite with large fruits and tolerance to fungal diseases.

Oat arabinoxylans and their potential glycemic index-lowering role: an in vitro study

ABSTRACT Oat (Avena sativa) is a cereal crop rich in dietary fibers, which are generally considered beneficial for human health. The objective of this work was to investigate the in vitro starch digestibility-reducing effect of the flour of a high arabinoxylan (AX) content (6.1%) oat line obtained from a random mutagenized oat population, compared to the original variety as a reference. The effect of the oat flours was tested on gelatinized potato starch as a dietary starch model, using a digestion dialysis method to calculate the predicted Glycaemic Index (pGI). The high AX flour reduced the potato starch pGI to larger extent than the flour from the original line. The pGI-lowering effect of the AX in the mutant line was confirmed by its attenuation following pre-treatment of the flour with xylanase. The larger pGI-lowering effect of the high AX oat line suggested this type of mutant as a potential functional food/ingredient for better management of postprandial glycemic responses.

УВОЛОГИЧЕСКАЯ ОЦЕНКА СТОЛОВЫХ СОРТОВ ВИНОГРАДА НА КОЛЛЕКЦИИ В 2022 ГОДУ

The paper presents the results of a study of the uvological indicators of table grapevine varieties growing on Don ampelographic collection named after Ya.I. Potapenko (Novocherkassk, Rostov region) in 2022. The varieties were studied in grafted culture (rootstock Kober 5BB). Planting scheme was 3.0×1.5 m. The study of grapevine varieties was carried out using methods generally accepted in viticulture and State Standards. The object of the research was 20 table and seedless grapevine varieties of various origin and maturation dates. Very high sugar content of berry juice had the varieties: Einset Seedless (25.4 g/100 cm3) and Nizina (23.5 g/100 cm3). Titrated acidity ranged from 3 g/dm3 (Esenin) to 7.6 g/dm3 (Bogotyanovskij). After taking measurements of bunches and berries, we note that the largest bunches had the varieties: Valentina, Kishmish Uzbekistan, Aku-Aku, Dolchatyj, Roshfor, Vostorg (length from 20.8 to 26.9 cm); the largest berries (in diameter) – Valentina, Bogotyanovskij, Talisman (from 25.3 to 29.5 mm). The highest average berry weight had the varieties ‒ Valentina (11 g), Bogotyanovskij (9.6 g), Talisman (8.9 g), Kardinal Ustojchivyj (8.1 g), Nizina (8.0 g). The following varieties received high degustation ratings: Kardinal Ustojchivyj (8.6 points), Roshfor (8.5 points), Aku-Aku (8.4 points), Talisman (8.1 points), control varieties – Dolchatyj (8.2 points). The lowest tasting rating among table varieties (at the level of 7.0‒7.5 points) had native varieties – Buryj and Efremovskij. Among seedless varieties, the following varieties received high tasting ratings: Kishmish Uzbekistan (8.8 points) and Einset Seedless (8.7 points), which is higher than the control variety Korinka Russkaya (8.3 points). According to the data of the conducted studies, table varieties were identified according to a set of morphological indicators ‒ Kardinal Ustojchivyj, Roshfor, Aku-Aku, Bogotyanovskij, Talisman, Valentina; among the seedless varieties are Kishmish Uzbekistan and Einset Seedless.

A novel wearable device for automated real-time detection of epileptic seizures

BackgroundEpilepsy is a neurological disorder that has a variety of origins. It is caused by hyperexcitability and an imbalance between excitation and inhibition, which results in seizures. The World Health Organization (WHO) and its partners have classified epilepsy as a major public health concern. Over 50 million individuals globally are affected by epilepsy which shows that the patient’s family, social, educational, and vocational activities are severely limited if seizures are not controlled. Patients who suffer from epileptic seizures have emotional, behavioral, and neurological issues. Alerting systems using a wearable sensor are commonly used to detect epileptic seizures. However, most of the devices have no multimodal systems that increase sensitivity and lower the false discovery rate for screening and intervention of epileptic seizures. Therefore, the objective of this project was, to design and develop an efficient, economical, and automatically detecting epileptic seizure device in real-time.MethodsOur design incorporates different sensors to assess the patient’s condition such as an accelerometer, pulsoxymeter and vibration sensor which process body movement, heart rate variability, oxygen denaturation, and jerky movement respectively. The algorithm for real-time detection of epileptic seizures is based on the following: acceleration increases to a higher value of 23.4 m/s2 or decreases to a lower value of 10 m/s2 as energy is absorbed by the body, the heart rate increases by 10 bpm from the normal heart rate, oxygen denaturation is below 90% and vibration should be out of the range of 3 Hz -17 Hz. Then, a pulsoxymeter device was used as a gold standard to compare the heart rate variability and oxygen saturation sensor readings. The accuracy of the accelerometer and vibration sensor was also tested by a fast-moving and vibrating normal person’s hand.ResultsThe prototype was built and subjected to different tests and iterations. The proposed device was tested for accuracy, cost-effectiveness and ease of use. An acceptable accuracy was achieved for the accelerometer, pulsoxymeter, and vibration sensor measurements, and the prototype was built only with a component cost of less than 40 USD excluding design, manufacturing, and other costs. The design is tested to see if it fits the design criteria; the results of the tests reveal that a large portion of the scientific procedures utilized in this study to identify epileptic seizures is effective.ConclusionThis project is objectively targeted to design a medical device with multimodal systems that enable us to accurately detect epileptic seizures by detecting symptoms commonly associated with an episode of epileptic seizure and notifying a caregiver for immediate assistance. The proposed device has a great impact on reducing epileptic seizer mortality, especially in low-resource settings where both expertise and treatment are scarce.

Drought resistance of soft spring wheat varieties of different ecological and geographical origins in the Forest Steppe of Ukraine

Drought resistance of soft spring wheat varieties of different ecological and geographical origins in the Forest Steppe of Ukraine