To reconstruct naturally and anthropogenically induced environmental change in SE Europe since the Little Ice Age, we have examined five partially varved sediment cores from coastal Lake Vouliagmeni, Greece. Our reconstructions are based on a multi-proxy approach including microfacies analysis, X-ray fluorescence core scanning, radionuclide dating (210Pb, 137Cs, and 241Am), and palynological analysis (pollen, spores, and dinoflagellate cysts). A 131 -cm-long composite record that encompasses the past c. 300 years reveals 181 varves of endogenic and mixed clastic-biogenic types. The formation of these varves was controlled by seasonal variability in clastic input and primary productivity. The non-varved intervals consist of homogenous lake sediments and turbidite deposits. Based on a chronology developed through radionuclide dating, varve counting and exclusion of turbidites from the sequence, we have compared our proxy data with meteorological data and historical records of earthquakes and human activities. Our results suggest that the surroundings of Lake Vouliagmeni experienced relatively wet conditions during the late solar Maunder Minimum (1645–1715 AD) and relatively dry conditions during the Dalton Minimum (1790–1830 AD), which highlights the hydroclimatic heterogeneity in SE Europe during the Little Ice Age. The evaluation of historical documentation suggests that the majority of the turbidites is related to lake-slope instabilities following earthquake shocks. Human impact on Lake Vouliagmeni includes (i) a change in aquatic biota following the artificial connection of the lake to the sea at c. 1880 AD, and (ii) expansion of agricultural areas and reduction of natural forests around the lake due to population growth over the past 300 years.