The aim of the present study was to optimize the manufacturing process of grapefruit seed extract (GFSE) incorporated Chitosan (0.75 %, 2 % w/w) and various corn starch concentration of CHCS film of ratio 2:1, 1:1, 1:2 respectively using sorbitol as plasticizer. Optimization of chitosan concentration in chitosan corn starch film was done on the basis of physicochemical properties e.g. moisture content, film thickness, solubility, water vapour permeability and characterization of the films were done. It was reported that the films with CH: CS ratio 2:1 had tensile strength values comparable to that of other films with CH: CS ratio 1:1 and 1:2. Hence, films with 2 % (w/w) chitosan and CH: CS ratio 2:1 was found best among all the film samples shelf life extended to 20 days. GFSE incorporated film was found to have better anti-microbial properties as compared to commercial polypropylene (PP) and low density polyethylene (LDPE) films. GFSE incorporated CHCS film of ratio 2:1 was applied on Grape fruits. The antimicrobial effect of coating formulation on grape was mathematically calculated by predictive kinetic models and digital imaging. Sensory evaluation of applied coating formulation was found very efficient and confirmed the shelf life enhancement of 20 days.
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