Uterine Fibroids Patients Research Articles

The mitochondrial DNA 4977-bp deletion and copy number alteration in Han Chinese samples with uterine fibroids.

Uterine fibroids (UFs) are the most common benign neoplasms, but their pathogenesis is not completely understood. Thus far, alterations in the mitochondrial DNA (mtDNA) content and the mtDNA 4977-bp deletion level in UFs, as well as the corresponding nontumorous tissue, have remained elusive. To test whether large mtDNA deletions and mtDNA content are involved in the pathogenesis of UFs, a total of 309 UF tissues and 28 paired adjacent myometrium from 270 UF patients were enrolled for the analysis of large mtDNA deletions and mtDNA content through the use of nested PCR and qPCR techniques, respectively. In our samples, a 4977-bp deletion was identified: 36 out of 309 UF tissues (11.56%) and 15 out of 28 (53.57%) paired adjacent myometrium were detected to harbor the 4977-bp deletion. In addition, a novel 4838-bp mtDNA deletion was identified in three UF tissues, and other different sizes of deleted fragments (4910, 4926, 5135-bp) were also found in UFs for the first time. Furthermore, older age was significantly associated with an mtDNA large deletion in the paired adjacent myometrium. We also found that increased mtDNA content and higher expression of ND1 occurred in solitary fibroids compared to adjacent myometrium. In conclusion, we identified a lower frequency of mtDNA large deletions and some novel large deletion in UFs for the first time. Furthermore, there was a general increase of mtDNA copy number during solitary UF development. Although the definite mechanism by which mtDNA was altered is supposed to be further confirmed, it will be helpful for further studies on the pathological mechanism of UFs.

Effect of HIFU on endometrial receptivity and sex hormone level in uterine fibroid patients and analysis of influencing factors for its treatment rate.

Effect of high intensity focused ultrasound (HIFU) uterine fibroid ablation on the endometrial receptivity and sex hormone level in uterine fibroid patients and the influencing factors for treatment rate were investigated. A retrospective analysis of 266 uterine fibroid patients admitted to the Department of Gynaecology in the Jining Maternity and Child Care Hospital from October 2013 to October 2016 was performed. Among them, observation group was treated with HIFU ablation (n=143), control group with myomectomy (n=123). The pulsatility index (PI) and the resistance index (RI) of the uterine arterial blood flow were measured during the luteal phase of menstruation by transvaginal ultrasonography. The serum luteinizing hormone (LH), follicle stimulating hormone (FSH) and estradiol (E2) were detected by chemical immunofluorescence. The relationship between HIFU treatment rate and clinical pathology of uterine fibroid patients was analyzed, and univariate/multivariate regression analysis was used to analyze the influencing factors for HIFU treatment rate. There was no significant difference in preoperative and postoperative PI and RI between the two groups (P>0.05); no significant difference between preoperative and postoperative PI/RI in the same group (P>0.05). There was no significant difference in preoperative and postoperative LH, FSH and E2 between the two groups (P>0.05); no significant difference between preoperative LH and postoperative LH in the same group (P>0.05), neither FSH or E2 (P>0.05). Results of multivariate analysis showed that fibroid location and ultrasound contrast intensity were independent influencing factors for HIFU treatment rate (P<0.05). Treatment of uterine fibroid with HIFU has no effect on the patient's endometrial receptivity and sex hormone level. Fibroid location and ultrasound contrast intensity are independent risk factors for HIFU treatment rate. This study provides guidance for the clinical optimization of treatment methods and is more conducive to the promotion of HIFU ablation therapy.

Laparoscopic surgery for early cervical squamous cell carcinoma and its effect on the micrometastasis of cancer cells.

The aim of this study was to evaluate the effect of laparoscopic radical hysterectomy on expressions of circulating tumor cells (CTCs) of cytokeratin 19 (CK19), cytokeratin 20 (CK20), and squamous cell carcinoma antigen (SCC-Ag) mRNA.We collect 78 patients with stage IA2-IIA1 cervical cancer who underwent radical hysterectomy by laparotomy or laparoscopy in our study, and 34 uterine fibroids patients and 32 healthy subjects were recruited as the positive control group and negative control group, respectively. Blood samples were taken from early-stage primary cervical squamous cell carcinoma patients. Real-time reverse transcription polymerase chain reaction (RT-PCR) was used to amplify peripheral blood CK19, CK20 and SCC-Ag from total RNA. We measured the expression of CK19, CK20, and SCC-Ag before laparoscopic radical hysterectomy, 24 hours and 30 days after surgery. Meanwhile, the expression of these markers was compared between laparoscopic and laparotomy groups.The expressions of CK19, CK20, and SCC-Ag in the experimental group before surgery were (0.0035 ± 0.0018), (1.06 ± 0.49), and (1.48 ± 0.46), respectively, and the positive rates were 32.1%, 33.3%, and 35.9%, respectively. The expression levels of CK19, CK20, and SCC-Ag in the experimental group before surgery was significantly higher than the positive and negative control groups, and there were no significant differences between the positive and negative control groups. The expressions and positive rates of CK19, CK20, and SCC-Ag before laparoscopic radical hysterectomy were significantly lower than the stage at 24 hours after surgery (P < .05), but higher than the stage at 30 days after surgery (P > .05). There were no significant differences in CK19, CK20, and SCC-Ag expressions before surgery, 24 hours and 30 days after surgery between laparoscopic group and laparotomy group (P > .05).Both laparotomy and laparoscopic radical mastectomy tend to increase the expression of CTCs in peripheral blood, and the expressions have no differences between these 2 groups. So, the use of CK19, CK20, and SCC-Ag expression levels from peripheral blood from early stage cervical cancer radical patients before hysterectomy can aid to overcome the lack of radiographic examination and tumor markers measurement, and provide clues for postoperative treatment and prognosis determination.

Serum cadmium level of uterine fibroid patients in Korea

Objective: The aim of this study is checking the blood level of cadmium (Cd) and lead in the patients with endometriosis or uterine leiomyomas. Materials and Methods: A total of 100 patients who underwent surgery for gynecological disease in the present tertiary teaching hospital were enrolled in this study. Twenty patients who underwent surgery for benign ovarian cysts were enrolled into the control group. Forty patients who underwent surgery for endometriosis and 40 patients who underwent surgery for leiomyoma were enrolled. Whole blood was collected by venipuncture in ordinary tubes after over eight hours of fasting. The Cd and lead levels were measured using graphite furnace atomic absorption spectrometry. Results: Blood lead and Cd levels were significantly higher in the leiomyoma group than in the control or endometriosis group. All participants were grouped into one of three groups according to their Cd level. Group 1 consisted of patients with Cd ≤ 0.05 ug/L (low quartile of the Cd level), group 2 included patients with 0.05 ug/L Conclusion: Blood Cd level was elevated in leiomyoma patients, not in endometriosis patients. Blood lead and Cd levels showed significant positive correlation.

Measurement of Uterine Leiomyomata Volume Pre and Post Uterine Artery Embolization Aided by MRI

Background: uterine fibroid embolization (UFE) is now evolving to be the first-line treatment for symptomatic uterine leiomyomata alongside the conventional surgical treatment of hysterectomy and myomectomy. Cochrane review comprises six RCTs comparing UAE versus hysterectomy or myomectomy for management of symptomatic leiomyomata where UFE had similar short and midterm outcomes, inclusive of symptomatic relief, quality of life and patient satisfaction, while benefiting from uterine preservation, shorter hospital stay and a faster recovery before resuming ordinary life activities. These benefits are in line with the minimally invasive nature of the embolization procedures. Aim of the Work: t this study aimed to review and illustrate the role of MR imaging in the pre- and post-procedural assessment for uterine fibroid patients undergoing uterine artery embolization. Patients and Methods: the current study was carried out in Radiodiagnosis Department, Faculty of Medicine, Ain shams University and specialized private radiology centers during the period between April 2017 and December 2017. It included 30 patients that were referred from gynecology clinic with their ultrasonographic reports to Radiodiagnosis Department, body imaging unit with a view to carry-out pelvic MRI with contrast. Results: imaging follow-up via pelvic MRI with contrast was obtained in 30 patients at 3, 6 months after treatment. The mean uterine volume was reduced by 25% (standard deviation was SD 0.03763) 3 months after treatment and was further reduced by a mean of 30% (SD 0.02638) by six months after treatment. Post-procedural subtraction MRI revealed mean myoma enhancement to be 5.6 + 0.5 SI in contrast to 133.4 + 18.3 SI prior to UFE. The mean dominant fibroid volume reduced by 34 % (SD 0.09247) after 3 months, 44 % (SD 0.05994) after 6 months. A cumulative reduction in both uterine and dominant leiomyoma mean volume from baseline to 6 months post – UAE was evident with significant interval changes between 3, 6 months follow up (p 0.000) Conclusion: the MRI findings following UAE vary with the interval from embolization and success of the procedure. MRI with its multiplanar capabilities was typically employed to evaluate the uterus following UAE for fibroid infarction, size reduction, location change, persistent enhancement, fibroid recurrence, changes in adenomyosis, and unexpected complications that may require surgical intervention or identify women who would benefit from repeated UAE. Recommendations: further studies on a larger scale of patients are needed to confirm the results obtained by this work.

Influence of laparoscopic hysteromyomectomy and hysterectomy on ovarian function in patients with uterine fibroids

Objective To observe the influence of laparoscopic hysteromyomectomy (LSHM) and hysterectomy (TH) on ovarian function in patients with uterine fibroids (UF). Methods 96 UF patients with accepted surgery in our hospital were selected.The patients were randomly divided into observation group and control group by random number table method, 48 cases in each group.LSHM was used for surgery in the observation group, while the control group accepted TH surgery.E2, AMH, FSH and LH levels before and after surgery, the operation time, intraoperative blood loss, flatus time and average length of stay, as well as proportion of perimenopausal symptoms in the two groups were recorded and compared. Results The operation time, intraoperative blood loss, flatus time and average length of stay in the observation group were (65.6±13.2)min, (82.4±6.2)mL, (20.4±4.6)h and (5.2±1.2)d, respectively, which in the control group were (76.5±15.6)min, (118.6±8.9)mL, (34.2±5.4)h and (8.3±1.2)d, the differences were statistically significant(t=3.695, P=0.00; t=23.120, P=0.00; t=13.482, P=0.00; t=12.650, P=0.00). The differences of E2, FSH, LH and AMH levels before surgery were not statistically significant (all P>0.05). After surgery, the E2 and AMH levels were significantly higher in the observation group(t=22.630, P=0.00; t=5.910, P=0.00), and FSH and LH levels were significantly lower in the observation group (t=6.853, P=0.00; t=7.024, P=0.00). The incidence rate of perimenopausal symptoms in observation group was 8.3% (4/48), which in the control group was 29.2% (14/48), the difference was statistically significant(χ2=6.838, P=0.00). Conclusion LSHM is helpful to protect UF patients' ovarian function, with faster postoperative recovery, and deserves further promotion in clinical practice. Key words: Laparoscopic hysteromyomectomy; Hysterectomy; Leiomyoma; Ovarian function

Influencing factors analysis for high intensity focused ultrasound ablation in treatment of uterine fibroids

This study aims to analyse the influencing factors for High Intensity Focused Ultrasound (HIFU) ablation in treatment of uterine fibroids. Fifty-one uterine fibroids patients with 76 uterine fibroids were enrolled in this study. All patients received the HIFU ablation of uterine fibroids. The treatment efficacy was evaluated. The residual rate of ablation was calculated. The influencing factors related to the treatment outcome of HIFU ablation were analysed. Results showed that, in 76 uterine fibroids, 25 (32.9%) cases obtained effective treatment outcome, with residual rate ≥ 50%, and 51 (67.1%) cases obtained remarkably effective treatment outcome, with residual rate<50%. The treatment outcome had significant difference among subserous, submucous and intramural fibroid type (χ2=6.614, P=0.037), among fibroid position of posterior wall, fundus uteri and anterior wall (χ2=11.410, P=0.003), between fibroid size of <5 cm and ≥ 5 cm (χ2=4.259, P=0.039), and among low, equal and high contrast enhancement (χ2=6.153, P=0.046). The logistic regression analysis showed that, the fibroid position was the independent risk factor of treatment outcome, with odds ratio of 2.545 (1.175-5.513). In conclusion, the fibroid type, fibroid position, fibroid size, and contrast enhancement are significantly related to the outcome of HIFU ablation in treatment of uterine fibroids. The fibroid position is the independent risk factor for treatment outcome.

Application of comprehensive project nursing in patients with hysteromyoma

Objective To observe the application effect of comprehensive project nursing in hysteromyoma patients. Methods Totally 68 hysteromyoma patients admitted from January 2015 to December 2016 were enrolled in this study. All patients were randomly divided into control group and observation group, with 34 cases in each. The control group was given routine nursing care, while the observation group was given comprehensive nursing project. The postoperative recovery of gastrointestinal function, clinical situation and complications were compared between the two groups. The psychological state at admission and discharge, the quality of sleep and the compliance of medical and nursing management were observed. Patients' satisfaction with nursing work were investigated and compared. Results All the indicators of gastrointestinal function in the observation group were recovered earlier than those in the control group, and the clinical observation indexes in the observation group were better than those in the control group. All the differences were statistically significant (P<0.05) . The psychological state of the two groups before discharge, the compliance of nursing treatment and the quality of sleep were all improved, and the evaluation indexes of the observation group were superior to those of the control group, with statistical differences (P<0.05) . In the observation group, there were 21 satisfied cases, 12 mostly satisfied cases and 1 not satisfied case, while there were 12, 17, 5 cases in the control group respectively (Z=-2.382, P=0.017) . The total incidence of postoperative complications was 5.88% in the observation group, which was significantly lower than 35.29% in the control group (χ2=8.995, P=0.003) . Conclusions The comprehensive nursing project can promote the recovery of gastrointestinal function in uterine fibroids patients after surgery, alleviate the psychological pressure of patients, improve patients' sleep quality and compliance of treatment and nursing care, reduce incidence of postoperative complications, so as to improve the satisfaction of patients with nursing work overall. Key words: Hysteromyoma; Sleep quality; Psychological state; Postoperative care; Compliance

Disability benefits as an incentive for hysterectomy: Uterine fibroid patients in Taiwan

ABSTRACTWorker compensation insurance in Taiwan ensures that a woman under the age of 45 years who has her uterus removed can receive disability compensation benefits. The present study investigated whether such a compensation policy was related to a woman’s inclination to have a hysterectomy. We extracted the records of 16,030 women diagnosed with uterine fibroids (UF) between 2000 and 2010 from the Longitudinal Taiwan Health Insurance Database. Each younger and older age group had a significantly lower hysterectomy rate compared to that of the 44-year-old age group. Moreover, significantly more patients with lower monthly wages had had hysterectomies than those with higher monthly wages. Policy makers should be aware that worker compensation regulations in Taiwan might encourage women with economic need to undergo hysterectomy surgery when approaching the age of 45 years.

Volumetric magnetic resonance-guided high-intensity focused ultrasound ablation of uterine fibroids through abdominal scars: the impact of a scar patch on therapeutic efficacy and adverse effects

BackgroundTo retrospectively compare the treatment success, therapeutic efficacy, and adverse effects of magnetic resonance-guided high-intensity focused ultrasound (MRgHIFU) treatment for uterine fibroid patients with and without abdominal scars.MethodsSeventy-six women who underwent treatment were divided into group 1 (patients with abdominal scars, which were covered with scar patches that prevents ultrasound energy from reaching the scar tissue immediately behind the patch) and group 2 (patients without abdominal scars). Non-perfused volume (NPV) ratios immediately after treatment, and fibroid volume reduction ratios and symptom severity scores (SSS) at the 6-months follow-up were assessed. All adverse effects were recorded.ResultsThe mean NPV ratios in groups 1 and 2 were 87.0 ± 14.1% and 91.5 ± 13.3%. At the 6-months follow-up, the fibroid volume reduction ratios in groups 1 and 2 were 0.45 ± 0.27 and 0.43 ± 0.21, and the corresponding improvement in mean transformed SSS were 0.7 ± 0.39 and 0.79 ± 0.28, respectively. No serious adverse effects were reported. The minor adverse effects encountered in this study are likely related to the temperature increase in the near-field of the ultrasound beam path, which inevitably leads to skin burns, or far-field heat absorption by distant bony structures (i.e., sciatic nerve symptoms), and are typically manifested inter-procedurally and resolved shortly thereafter.ConclusionsThe findings in this study suggest that the scar patch could be used safely and efficiently in MRgHIFU treatment for the patients with uterine fibroids and abdominal scars in the ultrasound beam path.

Inventory survey and influence factor analysis on the sleep quality of cervical cancer patients

Objective To understand about the sleep quality of cervical cancer patients, investigate the correlative factors that influence sleep quality, so as to provide the basis for improving the sleep quality of cervical cancer patients. Methods Cervical cancer patients whose postoperative tissue pathology was diagnosed as FIGO stage I and II were selected.7 days before and after operation, 1 month after operation in the follow-up visit (3 times), demographic questionnaire form, PSQI, CES-D self-rating depression scale, POMS-SF self-rating anxiety scale and cancer therapy function questionnaire (FACT-G) were used to have investigation and analysis on cervical cancer patients and hospitalized benign tumor (uterine fibroid) patients in the same term. Results Cervical cancer patients had no statistical difference from uterine fibroid patients on demographic questionnaire form.In different stages, PSQI aggregate score, CES-D depression, POMS-SF anxiety and FACT-G scores to the cervical cancer patients 7 days before and after operations, follow-up visit 1 month after operation had no significant statistical difference. However, compared with uterine fibroid patients, PSQI aggregate score, CES-D depression, POMS-SF anxiety and FACT-G scores of cervical cancer patients were higher 7 days before and after operations, follow-up visit 1 month after operation.However, the living quality score of FACT-G was lower than uterine fibroid patients, and the difference had statistical significance. Multivariate Logistic regression analysis indicated that marital status, educational level, working condition, family economic status, medical payment method, disease diagnosis and staging, pain score, depression, anxiety and living quality were obviously associated with the sleep disorder of cervical cancer patients. Conclusion The sleep quality of cervical cancer patients in different stages before operation and in the early stage after operation was inferior to benign tumor patients.In addition, sleep disorder occurrence rate of cervical cancer patients is higher.Sleep disorder can not only result in depression and anxiety easily, but also influence the living quality of patients. Key words: Cervical cancer; Sleep quality; Sleep disturbance

Validation of the Chinese version of the uterine fibroid symptom and health-related quality of life

Objective: To evaluate the reliability and validity of the Chinese version of the uterine fibroid symptom and health-related quality of life (UFS-QOL) in patients with uterine fibroid. Methods: The original English UFS-QOL was translated into Chinese and linguistically validated following the cross-cultural adaptation of health-related quality of life measures. Patients recruited randomly from Peking Union Medical College Hospital from June 2013 to December 2016 were scheduled for two visits with 2 weeks apart, and they were surveyed through the Chinese version of UFS-QOL and short-form 12-item health survey (SF-12). The reliability and validity of the Chinese version of UFS-QOL were evaluated. Results: A total of 190 uterine fibroid patients who met the criteria participated this study. The Chinese version of UFS-QOL had a high internal consistency (Cronbach's α, uterine fibroid symptom severity scale:0.912, health-related quality of life scale: 0.976) and high test-retest reliability (intraclass correlation coefficient, 0.572-0.951, P<0.01). The symptom severity scores and SF-12 scores were negatively correlated (r=-0.813, P<0.01); the health-related quality of life scores and SF-12 scores were positively correlated (r=0.620, P<0.01). The factor analysis showed good construct validity. Conclusion: Psychometric testing supports the reliability and validity of the Chinese version of UFS-QOL as an disease-specific measure of health-related quality of life.

Patient-reported prevalence and symptomatic burden of uterine fibroids among women in the United States: findings from a cross-sectional survey analysis.

PurposeTo estimate the prevalence of women diagnosed with uterine fibroids and the associated symptom burden in the US.Patients and methodsResponses of women aged 18–54 years, who completed an online survey, were analyzed. Data were weighted based on age, education, race, geographic region, income, and propensity score to derive national estimates of the prevalence of women diagnosed with uterine fibroids and associated symptom burden. Weighted means and percentages were reported. Prevalence across age and ethnic groups was examined. Symptom burden among women with and without uterine fibroids was compared using weighted logistic regressions.ResultsOf 59,411 respondents who met study inclusion criteria, 7.7% reported receiving a diagnosis of uterine fibroids. Of these, 5,670 women (1,402 in the uterine fibroid group and 4,268 in the control group) were excluded from analysis because they had a hysterectomy. Among the non-hysterectomized study participants, 3,031 self-reported a diagnosis of uterine fibroids (prevalence: 5.8%, 95% confidence interval [CI]: 5.5%–6.1%); prevalence increased as women aged and was greatest in the 50–54 age group (11.4%; 95% CI: 10.4%–12.4%). In addition, prevalence was greater in black vs white women (9.8%; 95% CI: 8.7%–11.0% vs 5.4%; 95% CI: 5.1%–5.7%). A greater percentage of women with uterine fibroids (vs those without) experienced severe heavy menstrual bleeding (16.7% vs 7.7%), severe constipation/bloating/diarrhea (7.7% vs 4.7%), severe passage of clots (6.7% vs 2.4%), severe spotting/bleeding between periods (1.7% vs 1.0%), and severe pelvic pressure (1.6% vs 0.6%). Among uterine fibroid patients with these typical uterine fibroid-related symptoms, 56.4%, 32.3%, 26.4%, 25.8%, and 20.4% reported heavy menstrual bleeding, passage of clots, spotting/bleeding between periods, constipation/bloating/diarrhea, and pelvic pressure, respectively, as extremely bothersome.ConclusionUterine fibroids impose a heavy burden on women aged 18–54 years in the US.

Perioperative psychological intervention for uterine fibroids patients

Objective The Maslow′s hierarchy concepts theory, in clinical uterine fibroids surgery patients, was used to analyze the effects of psychological intervention. Methods A retrospective analysis in our department from January to June 2015 was conducted in 76 uterine fibroids patients for their nursing care. Patients were divided into different groups for psychological intervention. Results The length of pain, time of hospitalization, medical cost, complication rates in the intervention group were lower than those in the conventional group; the satisfaction in the intervention group was high than that of the conventional group (P<0.05). Conclusions Nursing psychological intervention can help shorten hospital stays, reduce medical costs and promote early recovery of patients. Key words: Uterine fibroids; Hierarchy concepts theory; Psychological intervention; Peroperatiue period

Uterine artery embolization in uterine fibroids patients of reproductive age and its role in shaping of autoimmune oophoritis

In women of the reproductive age who have preserved the uterus after uterine artery embolization (UAE) and are interested in restoring fertility, the clinical evaluation of long-term results of this treatment method was executed with the use both of a questionnaire survey (n = 98), and determination of the status of ovarian reserve, ovaries, thyroid gland(n = 52). Performed retro- and prospective clinical-laboratory and instrumental methods of the investigation allowed to reveal a higher risk of the development of remote complications of UAE, in particular in the premature failure of ovarian function.

PIH3 - A Systematic Literature Review of Comorbidities and Symptoms among Uterine Fibroids Patients between 2000 and 2013

PIH3 - A Systematic Literature Review of Comorbidities and Symptoms among Uterine Fibroids Patients between 2000 and 2013

Diagnostic accuracy of peripheral blood Kisspeptin mRNA and plasma CA125 protein for detection of epithelial ovarian cancer in patients who have ever been pregnant.

To evaluate the diagnostic accuracy of peripheral blood kisspeptin (KISS1) mRNA and plasma cancer antigen 125 (CA125) protein of epithelial ovarian cancer (EOC) in previously pregnant patients, we prospectively enrolled 40 EOC patients as cases and 20 uterine fibroids patients with normal ovary as controls. Levels of peripheral blood KISS1 mRNA and plasma CA125 protein was respectively measured by RT-PCR and electrochemiluminescent method, respectively. Receiver operating characteristic curves with area under curve (AUC) were used to evaluate the diagnostic accuracy. Logistic regression analysis was used to obtain a prediction model for combined diagnosis of KISS1 mRNA and CA125 protein. Both KISS1 mRNA and CA125 protein and had good diagnostic accuracy for EOC, early EOC and advanced EOC (AUC > 0.5, P < 0.05). The CA125 protein had higher diagnostic accuracy than KISS1 mRNA for advanced EOC (P = 0.0009). Moreover, the combination of KISS1 mRNA and CA125 protein had higher diagnostic accuracy for EOC than them alone (P 0.05). Peripheral blood KISS1 mRNA was a novel biomarker for detecting EOC in previously pregnant patients. Combination application of KISS1 mRNA and CA125 protein was recommended for the diagnosis of EOC, but not for advanced and early EOC.

Differential plasma lipids profiling and lipid signatures as biomarkers in the early diagnosis of ovarian carcinoma using UPLC-MS

Biological requirements for tumor cell proliferation include the sustained increase of structural, energetic, signal transduction and biosynthetic precursors. Because lipids participate in the self-assembly of phospholipids to form biological membranes, they play important roles in membrane construction, energy storage, and cell signaling. We hypothesized that the differences in lipids between malignant ovarian carcinomas, benign ovarian tumors and normal controls could be reflected in the bio-fluids. A total of 215 pre-operative plasma samples were collected from 139 epithelial ovarian cancer (EOC), 38 benign ovarian tumor (BOT) and 38 uterine fibroid (UF) patients; these samples were then characterized by lipid profiling using ultra performance liquid chromatography/electrospray ionization mass spectrometry (UPLC–MS). The lipid profiles of the EOC and control samples (BOT/UF) as well as the different stages and histological subtypes of EOC were compared. Differentially expressed lipids were categorized as glycerophospholipids, sphingolipids and glyceroglycolipids and were found to be linked with three pathways. Almost all glycerophospholipids were down-regulated, especially phosphatidylcholine and phosphoethanolamine plasmalogens, whereas sphingolipids were up-regulated in the EOC patients compared to the controls. Lipids associated with pathological stage and histological subtype were also identified. We further identified five diagnostic lipids that were complementary to CA125. The predictive accuracy of five diagnostic lipids together with CA125 was higher than that of CA125 alone when distinguishing EOC and BOT/UF. In the future, the differential lipids identified may provide biologists with additional information regarding lipid metabolism and guide clinicians in making individualized therapeutic decisions if these results are confirmed in a larger study.

Identification of phosphatidylcholine and lysophosphatidylcholine as novel biomarkers for cervical cancers in a prospective cohort study.

Metabolites are the end products of cellular regulatory processes. Squamous cervical cancer (SCC) can alter the level of certain small molecular metabolite in plasma through modulating gene expression. In this study, we identified two metabolites, phosphatidylcholine (PC) and lysophosphatidylcholine (LPC), which are significantly down- and upregulated in plasma of SCC as compared to uterine fibroid (UF) patients via ultra-performance liquid chromatographic-mass spectrometry (UPLC-MS). In external prospective cohort, our assay has a sensitivity of 93.2%, a specificity of 91.3%, and an area under the receiver operating characteristic (ROC) curve (AUC) of 0.972. The level of LPC is significantly higher in SCC than in UF patients. An opposite result was observed in PC level. Our findings suggest that the PC and lysoPC could be used as novel biomarkers to facilitate SCC diagnosis.

Health guidance role in treatment of uterine fibroids in high intensity focused ultrasound ablation

Objective To explore the health guidance role on treating uterine fibroids in high intensity focused ultrasound thermal ablation(HIFU). Methods High intensity focused ultrasound ablation treatment was used in 74 cases of uterine fibroids patients before and after treatment to enable patients to better health guidance with treatment. Results After treatment,symptoms of patients with the clinical manifestation including menstrual too much,dysmenorrhea, frequent urination, constipation and anemia, were significantly improved. The volume of myoma of uterus before treatment and after treatment for 3 months,6 months and 1 year were(100.780±104.243)cm3,(53.446±49.546)cm3,(32.704±32.246)cm3 and(21.991±16.301)cm3, respectively. The volume of myoma of uterus gradually reduced after treatment as compared with that before treatment, the difference had statistical significance(t=6.758,186,7.567,all P<0.01). Conclusion Effective health guidance is beneficial to HIFU treatment for patient. Key words: High intensity focused ultrasound thermal ablation; Uterine tumor; Health education