The Biology of Forest Growth experiment was established in a 10-year-old Pinus radiata stand and comprised the following treatments: once-only fertilisation (400 kg N ha −1) with and without irrigation, a repetitive weekly application of liquid-soluble fertiliser applied with irrigation at rates ranging up to 20 kg N week −1, and unfertilised stands with and without irrigation. The treatment resulted in highly contrasting stands in terms of water and nutrient relations, rates of growth and allocation of biomass. Uptake of N from soil and litter, accumulation of N into foliage, subsequent retranslocation and resultant concentrations of N in each age class of foliage were measured in each stand over 4 years. Following fertilisation there was a rapid uptake of N into foliage and negligible net retranslocation within foliage during the subsequent 6 months. However, retranslocation of N out from ageing foliage became significant in the second and subsequent years and there was a positive linear correlation between the amount retranslocated and the total amount of N in the foliage of both irrigated and non-irrigated stands. Retranslocated N constituted 40–50% of N required for new foliage production. The sum of retranslocated N, and directly measured N taken up from soil, explained 98% of the variation in N accumulated into new foliage and 90% of the variation in new foliage production. However retranslocation was correlated with growth only if stands were irrigated. The peak foliage production of 8 t ha −1 year −1 resulting from fertilisation, required 130 kg N ha −1 year −1 which was derived from uptake from soil and retranslocation from older foliage. This was similar to the peak requirement of unfertilised 5- to 6-year-old stands on fertile sites in New Zealand. It was estimated that the duration of the growth response of trees associated with (a) initial uptake of fertiliser N from mineral soil, (b) re-mineralisation of N which was initially immobilised after fertilisation, (c) enhanced uptake from an N-enriched litter layer, and (d) retranslocation from older foliage, was likely to exceed 12 years.
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