Unsaturated Fatty Acid Composition Research Articles

The strengthening effect of a static magnetic field on activated sludge activity at low temperature

The strengthening effects of static (homopolar and heteropolar) magnetic fields (MF) on microorganisms were compared in activated sludge degrading organic matter at low temperature. The TTC dehydrogenase activity improved substantially through external heteropolar MF intensification, and led to the highest COD removal rate of 94.9% at 5°C. Phospholipid fatty acid analysis showed that C16:1ω7c, C18:1ω7c and C18:1ω9c were the essential unsaturated fatty acids in cell membrane at low temperature (4-15°C), accounting for the majority of the whole unsaturated fatty acids. The MF effect increased the Gram-negative bacteria content to improve the cold adaptability. Shannon-Wiener diversity analysis demonstrated the samples with heteropolar MF had a higher PLFA diversity index (1.17-1.25) than that with homopolar MF (0.89-1.13). AFM observation showed MF smoothed part of the microbial cell surface, with some remaining distinct protuberances. Heteropolar MF enhancement performance is much more effective than that of the homopolar MF with identical plate distance.

Effects of Dietary Bamboo Charcoal on the Carcass Characteristics and Meat Quality of Fattening Pigs

The purpose of this study was to investage the effects of dietary bamboo charcoal on the carcass characteristics and meat quality of fattening pigs. Fifty four crossed pigs of <TEX>$61.0{\pm}1.0$</TEX> kg body weight (BW) were grouped and housed in 6 animals (3 barrows and 3 gilts) per pen and 3 replications per treatment. The basal diet (C) was supplied with 0.3% bamboo charcoal as treatment 1 (T1) and 0.6 % as treatment 2 (T2). The pigs were fed that experimental diet for 42 days, thereafter 10 longissimus dorsi (LD) per treatment were randomly collected at the time the pigs reached an average weight of <TEX>$110.0{\pm}5.0$</TEX> kg. The carcass weight, backfat thickness, and the carcass grade were better (p<0.05) in the pigs fed bamboo charcoal than in C. The crude fat concentration of LD was higher (p<0.05) in T1 than in C. While the composition of stearic acid and arachidonic acid was lower (p<0.05) in treatments than in C, the composition of oleic acid and linoleic acid of treatments was higher (p<0.05) than C. The physico-chemical characteristics, such as meat color and amino acid composition of LD were not affected (p>0.05) by the supplemented bamboo charcoal. In conclusion, dietary supplementation with bamboo charcoal improved the carcass grade and fatty acids composition of pork meat from fattening pigs, where the composition of unsaturated fatty acids was increased, but that of saturated fatty acids was decreased.

사육방식에 따른 계란의 지방산 조성: 현장연구를 중심으로

This study examined the effects of cage rearing system and backyard raising system on fatty acid profile of egg in laying hens during 9 weeks. Two hundred hens, 70-wk-old, Hy-line Brown, were used in this study and fed basal diets. 100 hens, randomly collected from facility, were transferred to cage rearing system and 100 remaining hens were moved to backyard raising system. For overall period, higher unsaturated fatty acid percentages in two different rearing systems than did saturated fatty acid (SFA) were observed. In unsaturated fatty acid compositions, both rearing system showed a higher mono unsaturated fatty acid (MUFA) contents than poly unsaturated fatty acid (PUFA) contents. Egg quality traits of laying hens reared in backyard system had considerably lower contents of SFA and higher contents of MUFA compared with eggs from cage rearing system (P<0.05). In addition, eggs from cage rearing system resulted in a significantly lower n-3 contents and higher n-6:n-3 ratios and CLA contents in comparison with backyard raising system (P<0.05). However, no significant effects of two different rearing systems on PUFA, PUFA:SFA ratio, and n-6 contents were observed during the experimental period. In conclusion, this result suggests that cage rearing system and backyard raising system will play an important role in improving egg production as functional foods.

Nutrients balances and milk fatty acid profile of mid lactation dairy cows supplemented with unsaturated fatty acid

The objective was to evaluate the effect of unsaturated fatty acid sources supplementation on nutrients balances and milk fatty acid profile of mid lactation dairy cows. Twelve Brazilian Holstein cows in the mid lactation (mean of 128 days) and (580 ± 20kg of weight; mean ± SD) with milk yield of 25kg/d were assigned randomly into three 4 x 4 Latin square, fed the following diets: control (C); refined soybean oil; (SO); whole soybean raw (WS) and; calcium salts of unsaturated fatty acids (CSFA). Milk yield was 26.6; 26.4; 24.1 and 25.7 to the diets CO, SO, WS and CSFA respectively. Cows fed the WS treatment produced less milk (1.95kg/d of milk), fat and lactose than did cows fed the SO and CSFA. Cows fed the CSFA treatment showed less blood, urine (g/d) concentrations of N more energetic efficiency and intake of energy than did cows fed the SO treatment. Cows fed the unsaturated fatty acids sources showed more C18:2 cis-9, trans-11 CLA and trans-C18:1 FA concentration in milk than did cows fed the CO treatment. Diets with whole soybeans and soybeans oil provide more efficient digestive processes, and increase milk composition of unsaturated fatty acids.

Ultra‐endurance exercise differentially affects highly unsaturated fatty acid composition in cheek cells and serum phospholipids

The Western States Endurance Run is a 100‐mile trail footrace through the Sierra Nevada Mountains. We hypothesized that the event would generate enough reactive oxygen species (ROS) in runners to cause ‘membrane damage’ as evidenced by degradation of highly unsaturated fatty acids, primarily arachidonate (AA) and docosahexaenoate (DHA). Ten runners (7M/3F, aged 27 to 60 yr) with a completion time of 22.83±4.26 hours provided buccal (cheek) cell and serum phospholipid (PL) samples for determination of fatty acid composition at baseline, immediately post‐race, and days 1 and 2 post‐race. Consistent with our hypothesis, cheek cell AA decreased by 19% (P = 0.003) and DHA by 23% (P = 0.002) immediately post‐race. In contrast, serum PL AA increased by 15% (P = 0.005) and DHA increased by 8% (NS). The implications of a decrease in cheek cell AA and DHA remain unclear, but they could reflect a failure of cellular antioxidant defenses to manage the cumulative ROS burden associated with ultra‐endurance exercise. The increase in serum PL AA may reflect release from other pools such as adipose tissue or damaged skeletal muscle, or alternatively it could represent an attempt to deliver AA to aid in repair and regeneration of ROS‐mediated tissue damage. The speed of fatty acid changes suggest that cheek cells can be used to assess acute systemic oxidative stress.

Thermophile-fermented compost as a possible scavenging feed additive to prevent peroxidation

The extract of compost from fermented marine animals and thermophiles, including Bacillaceae, confers health benefits as a feed additive for fish and pigs. However, little research has explored how such compost extracts affect the physiological functions of the animals. In this study, the physiological effects of oral administration of the compost extract on the liver and muscle of rats are evaluated. After long-term administration of the compost extract in rats fed with either a normal diet or a high-fat diet over 3 months, accumulation of lipid peroxide and malondialdehyde, a marker of peroxidation, in the livers was reduced. Under such conditions, the unsaturated fatty acid composition in the liver was not significantly different in the rats fed either with or without the compost extract. In contrast, analyzes of 2,2-diphenyl-1-picrylhydrazyl (DPPH) revealed that free-radical-scavenging activity was increased in the livers of rats fed with the compost extract, although the extract itself had little of this activity. Glutathione, an antioxidant, was slightly increased following compost exposure. In addition, the levels of glutamate and glutamine, sources of glutathione, were slightly raised. Such a tendency was also observed in the muscle. Thus, thermophile-fermented compost can be a fermented feed additive to prevent peroxidation in the liver and muscle, and the effects of this additive may, in part, be associated with the retention of antioxidants and free amino acids within the organs.

Evaluation of Physicochemical Properties of Iranian Tomato Seed Oil

The major components of tomato processing industry wastes are seed that is not edible. The physicochemical properties and chemical composition of oil from tomato seed were studied by established methods. The oil yield of tomato seeds is about 35% on a dry weight basis. This vegetable oil has low sulphur (0.04 wt%), low ash content (0.034%), and high viscosity (51.5 mPa.s at 50°C). The fatty acid profile of tomato seed oil shows that there is a predominance of compounds containing an even number of carbon atoms, especially C16 and C18. The total saturated and unsaturated fatty acid composition is 18.28% and 81.72%, respectively, and the most abundant fatty acid is linoleic acid (56.12%). This study indicates that tomato seed wastes are a potential source of edible oil.

Comparison of Meat Quality and Fatty Acid Profile in Slow-Growing Chicken Genotypes Fed Diets Supplemented with Origanum Vulgare or Melissa Officinalis Leaves Under the Organic System

This study was conducted to compare the meat quality and selected fatty acids profile of two different slow-growing broiler genotypes (Hubbard S757; S757 and Hubbard Grey Barred JA; GB-JA) fed diets supplemented with dry oregano (Origanum vulgare L., OV; 10 g/kg basal diets) or lemon balm leaves (Melissa officinalis L., MO; 10 g/kg basal diets) under organic housing system. It is concluded that slow-growing genotypes had no effect on L* parameter of the breast, thigh and abdominal fat meat quality. Two hundred and forty chicks were allocated randomly into 4 experimental groups according to a 2×2 factorial arrangement. Birds were raised until 98 days in order to achieve an acceptable market live weight. The b* colours of breast and thigh meat were significant different among genotypes and also a* colour of breast meat of GB-JA increased (P<0.05). Slow-growing female broilers produced a higher dry matter content and lower fat content of breast meat as compared with males. There were the higher concentrations of linoleic (C18:2n-6) acid and the lower concentrations of linolenic acid (C18:3n-3) in genotypes fed with supplemented dry oregano or lemon balm leaves diet. Sex affected total unsaturated fatty acids (UFA) composition, polyunsaturated fatty acid (PUFA) and linoleic acid, were higher in slow growing males breast meat as compared with females breast meat. These results suggested that the slow-growing genotypes might had influenced the colour of breast and thigh meat, although overall meat quality was not affected under the organic system.

Physicochemical Characteristics and QTL Mapping Associated with the Lipid Content of High-Lipid Rice

This study was conducted to examine the physicochemical characteristics and perform QTL mapping of genetic factors associated with the lipid content of rice. A rice strain with a high lipid content, “P31-2-2-2-B-B”, was developed from mutants of “Dongjin” created by T-DNA insertion. The lipid content of “P31-2-2-2-B-B” brown rice was 4.42% whereas that of the donor cultivar “Dongjin” was 2.56%. The total fatty acid content of the high-lipid mutant brown rice was 7.82% and that of “Dongjin” was 3.43%. The unsaturated fatty acid composition of the mutant brown rice was 2.73% oleic acid, 2.74% linoleic acid, and 0.34% linolenic acid. In contrast, the fatty acid composition of the donor cultivar “Dongjin” was 1.30% oleic acid and 0.99% linoleic acid. The percentage of unsaturated fatty acid to total fatty acid in the high-lipid mutant was higher (74.3%) than that of “Dongjin” (66.8%). Continuous frequency distribution and transgressive segregation of the lipid content were observed in the F3 family (seeds) derived from a cross between the high-lipid mutant “P31-2-2-2-B-B” and a tongil-type cultivar “Samgang”. This result implied that the lipid content was a quantitative trait controlled by a polygene. Additionally, the broad sense heritability of lipid content was estimated to be 89.6% based on analysis of the F3 seeds. A significant QTL, qRLC5, was identified on chromosome 5 with a LOD score of 2.37, and was flanked by 5007 and 5014. Results of the present study should be useful for improving rice nutritional quality through marker-assisted selection.

요소 착물형성에 의한 포화지방산 고함유 팜유 및 우지 유래 바이오디젤의 저온유동성 개선효과 연구

Biodiesel is non-petroleum based fuel produced from vegetable oils or animal fats through transesterification. The compositions of saturated and unsaturated fatty acids in the feedstocks are important factors for biodiesel quality in terms of low-temperature fluidity and oxidative stability. The goal of this study is to improve the cold flow property of biodiesel from vegetable and animal origin containing highly saturated methyl esters (approx. 50%). In this purpose poly-saturated methyl esters in palm and tallow biodiesel were removed via urea-based fractionation and then the recovered fractions (enriched unsaturated fatty acid methyl esters) were supplemented with cold flow improvers. The highest concentration of unsaturated fatty acid methyl esters (93.8%) was obtained using a urea/fatty acid ratio of 3:1 at the crystallization temperature of <TEX>$0^{\circ}C$</TEX> for 17 hours in incubation, with recovery of 71% and the addition of cold flow improver (Flozol<TEX>$^{(R)}$</TEX> 515, 3,000 ppm) to the enriched poly-unsaturated fatty acid methyl esters reduced the CFPP(cold filter plugging point) of palm biodiesel from <TEX>$12^{\circ}C$</TEX> to <TEX>$-42^{\circ}C$</TEX>. In tallow biodiesel both the enrichment of unsaturated fatty acid methyl esters (93.71%) and the addition of cold flow improver (Infineum R408, 3,000ppm) reduced the CFPP from <TEX>$10^{\circ}C$</TEX> to <TEX>$-32^{\circ}C$</TEX>.

Biological accumulation of tellurium nanorod structures via reduction of tellurite by Shewanella oneidensis MR-1

The dissimilatory metal-reducing bacterium, Shewanella oneidensis MR-1, reduced tellurite (Te(IV), TeO32−) to elemental tellurium under anaerobic conditions resulting in the intracellular accumulation of needle shaped crystalline Te(0) nanorods. Fatty acid analyses showed that toxic Te(IV) increased the unsaturated fatty acid composition of the lipid components of the cell membrane, implying a deconstruction of the integrity of the cellular membrane structure. The current results suggest that dissimilatory metal reducing bacteria such as S. oneidensis MR-1 may play an important role in recycling toxic tellurium elements, and may be applied as a novel selective biological filter via the accumulation of industry-applicable rare materials, Te(0) nanorods, in the cell.

The stearoyl-acyl-carrier-protein desaturase promoter (Des) from oil palm confers fruit-specific GUS expression in transgenic tomato

The stearoyl-acyl-carrier-protein (ACP) desaturase is a plastid-localized enzyme that catalyzes the conversion of stearoyl-ACP to oleoyl-ACP and plays an important role in the determination of the properties of the majority of cellular glycerolipids. Functional characterization of the fatty acid desaturase genes and their specific promoters is a prerequisite for altering the composition of unsaturated fatty acids of palm oil by genetic engineering. In this paper, the specificity and strength of the oil palm stearoyl-ACP desaturase gene promoter (Des) was evaluated in transgenic tomato plants. Transcriptional fusions between 5′ deletions of the Des promoter (Des1–4) and the β-glucuronidase (GUS) reporter gene were generated and their expression analyzed in different tissues of stably transformed tomato plants. Histochemical analysis of the Des promoter deletion series revealed that GUS gene expression was confined to the tomato fruits. No expression was detected in vegetative tissues of the transgenic plants. The highest levels of GUS activity was observed in different tissues of ripe red fruits (vascular tissue, septa, endocarp, mesocarp and columella) and in seeds, which harbored the promoter region located between −590 and +10. A comparison of the promoter-deletion constructs showed that the Des4 promoter deletion (314bp) produced a markedly low level of GUS expression in fruits and seeds. Fluorometric analysis of the GUS activity revealed a 4-fold increase in the activity of the full-length Des promoter compared to the CaMV35S promoter. RNA-hybridization analyses provided additional evidence of increased GUS expression in fruits driven by a Des fragment. Taken together, these results demonstrate the potential of the Des promoter as a tool for the genetic engineering of oil palms and other species, including dicots, in improving the quality and nutritional value of the fruits.

Roselle (Hibiscus sabdariffa L.) seeds as unconventional nutritional source

The composition of roselle seed from oil, protein, ash, fiber, fatty acids and amino acids was determined and compared in three cultivars in order to use it as an unconventional nutritional source. Aswan cv. occupies the highest significant rank in protein (31.51), oil (23.70) and fiber (4.87%) contents. Aswan and Sewa cvs. had the highest significant unsaturated fatty acid composition, especially oleic and linoleic acids, with oleic acid having values of 36.22 and 33.34% and linoleic acid, 14.95 and 15.10% values. Protein of Aswan cv. had the highest significant values of seven essential amino acids and four non-essential amino acids, especially lysine and phenylalanine. Key words: Amino acids, fatty acids, nutrition, oil, protein, roselle, unconventional crops.

Ruminal microbe of biohydrogenation of trans-vaccenic acid to stearic acid in vitro

BackgroundOptimization of the unsaturated fatty acid composition of ruminant milk and meat is desirable. Alteration of the milk and fatty acid profile was previously attempted by the management of ruminal microbial biohydrogenation. The aim of this study was to identify the group of ruminal trans-vaccenic acid (trans-11 C18:1, t-VA) hydrogenating bacteria by combining enrichment studies in vitro.MethodsThe enrichment culture growing on t-VA was obtained by successive transfers in medium containing t-VA. Fatty acids were detected by gas chromatograph and changes in the microbial composition during enrichment were analyzed by denaturing gradient gel electrophoresis (DGGE). Prominent DGGE bands of the enrichment cultures were identified by 16S rRNA gene sequencing.ResultsThe growth of ruminal t-VA hydrogenating bacteria was monitored through the process of culture transfer according to the accumulation of stearic acid (C18:0, SA) and ratio of the substrate (t-VA) transformed to the product (SA). A significant part of the retrieved 16S rRNA gene sequences was most similar to those of uncultured bacteria. Bacteria corresponding to predominant DGGE bands in t-VA enrichment cultures clustered with t-VA biohydrogenated bacteria within Group B.ConclusionsThis study provides more insight into the pathway of biohydrogenation. It also may be important to control the production of t-VA, which has metabolic and physiological benefits, through management of ruminal biohydrogenation bacterium.

Hydrocarbon and Fatty Acid Composition of Cheese As Affected by the Pasture Vegetation Type

The determination of the geographical origin of dairy products is an ongoing issue. In this paper the effects of botanical diversity of two pastures on the hydrocarbon and fatty acid composition of cheese fat were studied, over 2 years of experimentation. Two areas in the Italian southwestern Alpine region, dominated by Trifolium alpinum (T) and Festuca nigrescens (F) vegetation, respectively, were chosen, and milk obtained from cows grazing on these pastures was used to produce a semihard traditional cheese. Cheese samples showed a significantly different composition of most linear hydrocarbons, odd-chain (C15, C17, and C17:1) and unsaturated (trans-11,cis-15-C18:2, C18:3, C20:4n-6, C20:4n-3, and 20:5n-3) fatty acids, according to pasture type. The ratio between C(29) and C(27) linear hydrocarbons, unlike the absolute content of the single molecules, showed a good discriminating ability between the two pastures and was little affected by the natural variability due to the climatic and environmental factors.

Effects of β-carotene on the thermal oxidation of fatty acids

β-Carotene is one of the most important fat soluble pigment with well known antioxidant and provitamin A activities. It is used in industries as food colorant and a source of vitamin A. The thermal induced degradation during processing leads to color and properties losses. The thermal stability of the fatty acids composition of edible oils is of great importance to food manufacturers. Corn oil, rapeseed and sunflower oils were fortified with 50 to 300 μg/g of β-carotene and oxidized using Rancimat (air flow rate 20 L/h) at 110°C for 14 h. Fatty acid methyl esters (FAMEs) were measured using gas chromatography with Agilent-Technologies DB-Wax capillary column. It was found that by adding β-carotene (50 to 300 μg/g) to the corn, rapeseed and sunflower oils, no significant changes was observed in saturated fatty acids. Saturated fatty acids were relatively more protected in the presence of unsaturated fatty acids of similar carbon atoms and in the presence of β-carotene. The addition of β-carotene affected the composition of unsaturated fatty acids in the tested oils. Thus, β-carotene acts as a pro-oxidant in highly unsaturated sunflower oil. Unsaturated fatty acids are oxidized earlier and results to the formation of unpleasant flavor and consequent rancidity. Key words: β-Carotene, lipids oxidation, thermal stability, edible oils, fatty acids composition.

Fatty acid profile of &amp;lt;i&amp;gt;Longissimus dorsi&amp;lt;/i&amp;gt; muscle of crossbred pigs fed with addition of conjugated linoleic acid or sunflower oil

Abstract. The aim of the paper was to investigate the impact of feeding pigs with different levels of conjugated linoleic acid or sunflower oil on fatty acid profile of Longissimus dorsi muscle. The subjects of research were 60 crossbred gilts divided into 6 groups, fed with different levels of conjugated linoleic acid (CLA) or sunflower oil (SFO) (0.5; 1.0 and 2.0 %, respectively). All fatteners were kept and fed under standardized conditions. Animals were slaughtered at 95 kg of body weight. Fatty acid profile was determined in samples of Longissimus dorsi muscle from each animal. Gas chromatography was used (in the research). The significance of differences between groups was verified by Duncan’s test. In the present study the addition of conjugated linoleic acid or sunflower oil did not impact the composition and amounts of saturated or unsaturated fatty acids in Longissimus dorsi muscle.

Long term storage stability of Jatropha curcas biodiesel

The objective of this work was to study the long term storage stability of JCB ( Jatropha curcas biodiesel). For the objective JCB was mixed with PY (Pyrogallol) and different metal contaminants. JCB samples were stored for 6 months in open air exposed to sunlight. Different properties were checked with respect to time. It was found that stability of fresh JCB was not acceptable as per EN 14214. When PY was mixed with JCB, it stability increased and 200 ppm of PY was sufficient to make fresh and pure JCB stable for almost 6 months. Viscosity (n), PV (peroxide value) and AV (acid value) increased with respect to time. Unsaturated fatty acid composition was also checked with respect to time. From the experiment it was clear that as oxidation deterioration advanced, linoleic and linolenic acid methyl esters decreased and the fraction of oleic acid methyl became relatively high with respect to storage time.

Thermal regulation of membrane lipid fluidity by a two‐component system in Bacillus subtilis

This article describes a simple and robust laboratory exercise on the regulation of membrane unsaturated fatty acid composition in bacteria by a decrease in growth temperature. We take advantage of the well characterized Des pathway of Bacillus subtilis, composed of a Δ5-desaturase (encoded by the des gene) and the canonical two-component system DesK-DesR, to study the transcriptional regulation of des during cold shock. Students analyze the expression of a reporter transcriptional fusion between the des promoter and the bacterial lacZ gene in a wild-type B. subtilis strain and in des or desK-desR mutants grown under different culture conditions. Measurements of β-galactosidase activity allow them to investigate how the Des pathway works and to assess the role of each component of this regulatory system.

한우육의 성분조성에 미치는 인진쑥 TMR 사료 급여 영향

본 연구에서는 제주도에서 자라는 인진쑥을 일정량 첨가한 TMR 사료를 개발하여, 이를 제주 한우에게 급여한 경우, 인진쑥의 급여가 한우등심의 성분조성에 미치는 영향을 확인하였으며, 그 결과로 고급화된 기능성 한우의 생산 가능성을 검토하였다. 대조군(control)은 인진쑥을 혼합하지 않은 비육우 사료를 급여하였고, 급여군(treatment)은 인진쑥을 혼합한 비육우 사료를 급여하였다. 한우등심의 수분, 조단백질, 조지방, 조회분 함량, 열량, 콜레스테롤 및 콜라겐은 인진쑥 급여에 의한 영향이 없었다. 무기질 함량은 대조군 및 급여군 사이에 유의한 차이가 없었으며, 총무기질 함량은 대조군 및 급여군이 각각 3,559.20 및 3,998.01 ppm으로 유의한 차이가 없었다. 비타민 <TEX>$B_1$</TEX> 및 <TEX>$B_2$</TEX>함량은 대조군 및 급여군 사이에 유의한 차이가 없었다. 구성아미노산 총량은 대조군 및 급여군이 각각 14.56% 및 13.90%로 유의한 차이가 없었으며, 유리아미노산 총량은 대조군(763.06 ppm)과 급여군(720.92 ppm) 사이에 유의한 차이가 없었다. 포화지방산은 대조군 및 급여군이 각각 42.956% 및 40.918%이고, 불포화지방산은 각각 57.043% 및 59.083%이었다. 인진쑥을 급여한 한우등심에서 유의하게 증가한 지방산은 myristic acid, myristoleic acid, pentadecenoic acid, palmitic acid 및 <TEX>${\gamma}$</TEX>-linolenic acid이었으며, 급여하지 않은 한우등심에서 많이 함유된 지방산은 arachidic acid이었다(p<0.05). TMR feed developed by adding mugwort originating from Jeju Island,was fed to Hanwoo to investigate the effects of feeding mugwort on nutritional composition, as well as the feasibility of producing Hanwoo beef with high quality and functionality. The samples for the experiment consisted of Hanwoo fed fattening cattle TMR feed without mugwort (control), and Hanwoo fed fattening cattle TMR feed with added mugwort (treatment). Feeding mugwort did not lead to a significant difference in the moisture, crude protein, crude fat, crude ash, calorie, cholesterol, and collagen contents. There was no significant difference between the control and treatment groups in mineral components. The control and treatment groups had total mineral amounts of 3,559.20 and 3,998.01 ppm, respectively. The total amount of structural amino acids did not show any significant difference between the control (14.56%) and treatment groups (13.90%). The total amount of free amino acids did not show any significant difference between the control (763.044 ppm) and treatment groups (720.885 ppm). The composition of saturated fatty acids was 42.956% for the control and 40.918% for the treatment group. Further, the composition of unsaturated fatty acids was 57.044% for the control and 59.082% for the treatment group. Myristic acid, myristoleic acid, pentadecenoic acid, palmitic acid, and <TEX>${\gamma}$</TEX>-linolenic acid were increased by feeding mugwort. Arachidic acid was contained the most among the fatty acid of Hanwoo fed feed without mugwort (p<0.05).