University Scientific Research Projects Research Articles

Correction to: Determination of Reference Intervals of Biochemistry Parameters in healthy individuals in Gaziantep Province

In the first published version of this article [1], the authors stated that "Funding: The authors declare no funding" was misspelled. This has been changed to "Funding: The study was supported by Gaziantep University Scientific Research Projects Unit with project number TF.11.36". The authors take full responsibility for this confusion, and they apologize for the confusion. Publisher’s Note: The original article has been corrected, and a correction note was added. The original article can be find online at; https://doi.org/10.58600/eurjther.20232902-1343.y

Effect of intra-abdominal boric acid in the experimental adhesion model.

The continuous advancement in medical and surgical techniques has led to a rise in the frequency of abdominal operations, subsequently increasing the incidence of intra-abdominal adhesions. Over 90% of laparotomies result in postoperative intra-abdominal adhesions. This study investigates the effect of a 5% boric acid solution on the development of intra-abdominal adhesions in rats, using an adhesion model. This study was conducted with two groups: a control group, in which the adhesion model was applied without any treatment, and a boric acid group, which was treated with a 5% boric acid solution. Each group comprised 16 rats. On the 14th postoperative day, the rats were sacrificed, re-explored, and the developed adhesions were evaluated both macroscopically and microscopically. The data from macroscopic and microscopic scoring were analyzed using the Mann-Whitney U test in the IBM Statistical Package for the Social Sciences (SPSS) Statistics 24 program. A p-value of less than 0.05 was considered statistically significant. This research was supported by the Manisa Celal Bayar University Scientific Research Projects Commission. A statistically significant difference was observed between the boric acid-treated group and the control group, with the boric acid group showing a significant decrease in adhesion development both macroscopically and microscopically (p<0.05). In the future, boron could play a significant role in reducing and preventing intra-abdominal adhesions after surgery. This investigation could pave the way for further research into the mechanism by which boric acid prevents the development of intra-abdominal adhesions. Moreover, it is imperative to explore the potential side effects of intra-abdominal boron application at the optimum concentration of the solution.

Possibilities of Using “BUGA” Named Software for Breeding Value Estimation of Anatolian Water Buffalo Population of Istanbul

In this study, variance components and breeding value estimations of Anatolian water buffaloes were estimated from the data obtained within the scope of community based water buffalo improvement program of Anatolian water buffalo population of İstanbul. The "BUGA" named software, which was started to be developed with the support of Harran University Scientific Research Project Unit (HUBAP) for use in the estimation of (co)variance components and breeding value were used in this study. The BUGA software used in the research can predict genetic and environmental effects together with variance components as Best Linear Unbiased Prediction (BLUP) with the Restricted Maximum Log likelihood (REML) method. Unlike its counterparts of another software of foreign origin, it has a visual interface. The language of use is Turkish. The dataset includes records such as city, district, village, genealogy information, date of birth, gender, calving date, lactation parity, number of test day milk yield, lactation milk yield and previous calving date of 442 Anatolian buffaloes raised in this population. The records were analysed using the repeatability model with BUGA software and the additive genetic and permanent environmental effects of all animals in the dataset were estimated. Random environment variance, genotypic variance and permanent environment variance values were estimated as 107112.60 kg2, 20045.22 kg2 and 1259.50 kg2, respectively, while heritability (h2) and degree of repeatability (r) were determined as 0.1561 and 0.1659, respectively. The results obtained as an example of case study applied for the possibility of usage the BUGA software in the estimation of genetic parameters and breeding values showed that BUGA software can be easily used in farm recording conditions with the advantage of friendly user features.

Use of service robots in hospitality: An observational study in terms of technology acceptance model

This study aims to explore hospitality customers’ behavioral intentions and reactions toward service robots from the perspective of the Technology Acceptance Model. Kastamonu University Scientific Research Projects Coordination Department has supported this research. In this context, the service robot was leased. The service robot has been shown to customers in the lobbies, restaurants, rest areas, and cafes of accommodation establishments. Customers were enabled to interact with the robot. The participant observation method was adopted in the study. Unstructured interviews were conducted. The data divided into categories from the perspective of the Technology Acceptance Model were processed using the MAXQDA analysis tool. The density map was drawn from the “Document Portfolio.” The data were obtained as a result of participant observation. It is divided into two categories. These; are “Behavioral Responses” and “Cognitive and Emotional Responses.” The most prominent themes within the categories are video/photo taking, speaking, touching (behavioral), excitement, liking, and having fun (cognitive and emotional). The user rate of these reactions is 7/10 on average. It was observed that the participants enjoyed, were satisfied, and wanted to interact with the service robot. These findings reveal that behavioral intentions related to technology acceptance may occur against service robots.

Optimizing HR management system on attention deficit in faculty work

BackgroundWith the increase of occupational stress and workload, more and more occupational people have problems of work attention deficit. The HRM system will be helpful to the occupational group, so it can be used in the study of the work attention deficit of faculty members.Subjects and Methods60 teaching staff of a vocational college were selected as the research subjects and randomly divided into experimental and control groups, with 30 people in each group. The experimental group applied the optimized human resource management system; the control group was managed according to the unoptimized system. Memory load n-back task was used and relevant experimental data were recorded; Pearson was used to analyze the relevant data.ResultsThe mean reaction time of the experimental group in the 1-back task was (505±100) ms, while that of the control group was (457±75) ms, and the difference between the groups was statistically significant after repeated-measures ANOVA (P=0.017). In the 2-back task, the mean reaction time of the experimental group was (573±125) ms, while the control group was (511±100) ms, and the difference between the groups was statistically significant (P=0.016).ConclusionsFaculty members optimized for the HRM system showed longer reaction time and increased ISD when completing n-back tasks, and these differences were associated with clinical symptoms of attention deficit at work. Therefore, HRM system optimization research can explore additional interventions to improve faculty members’ level of work attention.AcknowledgementGuangzhou Education Bureau’s University Scientific Research Project - Youth Talents Scientific Research Project (No. 202032782); Research Project of Guangzhou Education Research Institute (No. 21AZYJY21126).

O.2.3-9 The working shifts &amp; physical literacy factors associated with physical activity level in various settings among nurses: preliminary results

Abstract Purpose This study aims to examine (I) the importance of working shifts on physical activity (PA) level and (II) the association between physical literacy (PL) and energy spent with PA in occupational, leisure-time, and sports settings among nurses who live most of their time in healthcare environments and are aware of health literacy and PA. Methods The data of this cross-sectional study were collected from 201 nurses (MAGE= 34.53±8.41; MBMI= 24.32±4.17) nurses working at hospitals in Eskisehir. Woking shifts were 08:00-16:00 (n = 53), 16:00-08:00 (n = 19), alternating between 08:00-16:00 and 16:00-08:00 (n = 47), and 24-hour shifts with a day off between shifts (n = 82). The 7-Day PA Assessment Questionnaire developed by Karaca and Turnagöl (2007) was used as a data collection tool as it enables individuals to report their various types of physical activities (occupational, leisure-time, sport) in the past seven days, and the energy spent is computed in terms of MET. Moreover, the Turkish version of the PL Questionnaire (Munusturlar and Yıldızer, 2021) was used to measure PL level in knowledge, communication, and self-awareness subdimensions. The data were analyzed with One-way ANOVA and multiple linear regression models. Results The one-way ANOVA results indicated nurses working in 24-hour shifts reported significantly higher MET values (276.05±71.07 MET/week) in occupational PA than nurses working between 08:00-16:00 (222.00±53.70 MET/week) and 16:00-08:00 (223.27±73.67 MET/week) shifts, F(3, 200)=8.539, p &amp;lt; 0.001. Regression models indicated that PL was only associated with spent energy in sports activities [F(4,200)=9.21, p &amp;lt; 0.001], but not with occupational and leisure-time PA. Lastly, knowledge was the only PL factor associated with energy spent on sports activities (ß=1.35, OR:0.77-1.93). Conclusions 24-hour working shifts with day-offs increase energy expenditure with occupational physical activity, however, there was no significant differences were found in energy expenditure with leisure time or sports activities in favor of those who work in these shifts. Moreover, increased physical literacy knowledge is the only factor for increasing physical activity participation through sports activities. Hence, policies, PA prescriptions, and interventions should focus on PL knowledge and understanding. Support/Funding Source This study was supported by Eskişehir Technical University Scientific Research Projects Commission under grant no: 23LÖT069.

Age‐related cognitive decline are manifested by decreased event‐related delta and theta oscillations

AbstractBackgroundEvent‐related oscillations (EROs) during cognitive paradigms reflect several cognitive functions in the brain. Event‐related delta responses are related to signal detection and decision making processes whereas theta is associated with working memory and selective attention (Yener and Başar, 2013; Başar et al., 2016). During physiological aging, EROs may be altered. The aim of the current study was to investigate time‐frequency measurements of event‐related oscillations in younger and older healthy participants.MethodFifty‐nine healthy participants were included in this study. They were divided into two subgroups as younger and older according to the age median score. Thirty younger healthy participants (mean age 22.37 years; standard deviation: 2.85) and 25 older healthy adults (mean age 56.48 years; standard deviation: 9.71) were completed a visual oddball task during EEG recording. Event‐related band power was assessed using event‐related spectral perturbation. Inter‐trial coherence values were analyzed by phase‐locking measurements. Event‐related power and phase‐locking analysis were performed for delta (0.5‐3.5 Hz), and theta frequency band (4‐7 Hz) over frontal (F3, Fz, F4), central (C3, Cz, C4), parietal (P3, Pz, P4), occipital (O1, Oz, O2) electrode locations. Repeated measures of ANOVA were used for statistical analysis.ResultThere were main GROUP effects for both event‐related delta [F(1,53) = 6.328, p = .015, ηp 2 = .107, Figure 1] and theta power [F(1,53) = 28.069, p&lt;.001, ηp 2 = .346, Figure 2], indicating older healthy adults had reduced power values compared to younger healthy individuals (for all; p&lt;.016). Furthermore, an interaction effect of Anterior‐Posterior Electrode Location x Group was found for theta power [F(3,159) = 13.869, p&lt;.001, ηp 2 = .207], showing older healthy individuals had reductions in all electrode locations (for all; p&lt;.017). Lastly, event‐related theta phase‐locking values were found to be reduced in older healthy adults [F(1,53) = 5.512, p = .023, ηp 2 = .094, Figure 3].ConclusionThe present study demonstrated older healthy participants had decreased event‐related delta and theta responses during visual oddball paradigm. These reductions may be explained by the alterations in cognitive capacities such as working memory/attention and decline in processing information during healthy aging. Acknowledgement: The inclusion of younger healthy participants in this study was supported by Ankara University Scientific Research Projects Coordination Unit (Project Number: 16L02000001).

Akıllı Kampüs Konsepti Altında Dijital Süreçler İçin Süreç Madenciliği Metodolojisi

Digital transformation affects universities as well as many industries. Universities are increasingly using various digital resources and systems to manage their knowledge. The smart campus, on the other hand, supports informed decision-making by integrating these resources and systems. Process mining provides real insights for digital transformation, allowing processes to be examined more transparently. This study aims to examine the proposed project implementation processes related to the smart university with the process mining methodology. For this purpose, 32 completed projects submitted to İzmir Bakırçay University Scientific Research Projects Coordinatorship (BAPK) with the proposed methodology adapted from Deming's continuous improvement cycle were examined. The data are taken from two different pages in the project automation system. According to the research findings, Projects are grouped into three categories: Guided Projects (GDM, 5 projects), Graduate Thesis Projects (TEZ, 5 projects), and Career Start Support Projects (KBP, 22 projects). 40.6% (13 projects) of the applications went directly to the project review stage, while 19 (59.4%) needed procedural correction. Considering the time from the creation of the application of 32 projects to the signing of the contract, it is seen that the arithmetic average of the cycle time is 15.1 weeks, and the median average is 52.5 days. The notable difference between arithmetic and median mean is that very few projects are of long duration. Procedural adjustments affect project evaluation cycle time by an additional 14 days. The carelessness or lack of knowledge of the applicants extends the cycle time of the process from 15 days to 53 days. The total duration of unnecessary waiting time in the process is 17 days. This study primarily proposes that non-digital processes should be digitized as soon as possible.

PO33 Presentation Time: 10:30 AM: Testing the Usability of the Vaginal Speculum Tongue Made of Polyetheretherketone Material in Brachytherapy by Dosimetry Method

<h3>Purpose</h3> In the tandem + ovoid brachytherapy application, during the metallic speculum removing process after the vaginal packaging process, the positions of the sponges may change due to the spoon-shaped structure of the speculum, and this situation may cause not desired efficiency from the packaging process. In this project, we aimed to 3D printing of vaginal speculum tongues from polyetheretherketone (PEEK) material and make the dosimetric evaluation to assess usability during packing in gynecologic brachytherapy. <h3>Materials and Methods</h3> In this study, we designed a modular structure in which the upper and lower tongue can be easily separated from the handle similarly to the metallic vaginal speculum we use in our routine practice with no requirement to remove the speculum tongues from the patient after packaging. The tongues of the designed speculum were produced with PEEK material with a layer thickness of 200 microns using a 3D printer (Intamsys Technology Co. Ltd., Shanghai, China). To evaluate the radiation permeability of the produced speculum tongues, a phantom containing water was designed to perform dosimetric studies and 2 different dosimetric methods were used such as the treatment planning system (TPS) and Radiochromic Film Dosimeter system (Gafchromic: EBT3). TG43 and ACUROS BV algorithms are used as TPS. Prescribed dose (3 Gy) at the surface of phantom (side with the speculum) was calculated for a single source Dwell position in TPS using with ACUROS BV algorithm. The irradiation time was 631.1 seconds for both algorithms. The activity of the radioactive source was 11,086 Ci. The measured values that were obtained from the same points with different techniques were recorded and relations with each other were evaluated by using the Pearson Correlation test. In each measurement method, dose values were recorded in 4 different directions such as right (with no speculum), left (with speculum), up, and down. Dose-distance graphs were created for each direction from these values, and no break in the curve was detected on the side of the speculum tongue in phantom. This work was supported by Eskisehir Osmangazi University Scientific Research Projects Unit as project number TSA-2021-2143. <h3>Results</h3> The measurement values obtained from two different TPS algorithms and the film dosimetry method are given in Figure 1, and no significant difference was found in the statistical evaluation. The values obtained by using different methods show parallelism with each other, and no breakage was detected due to the speculum tongue. <h3>Conclusions</h3> In conclusion, all values obtained from TPS and radiochromic film dosimetry were parallel to each other. When the dose values at the same points and dose-distributions were evaluated, we concluded the speculum tongues we produced from PEEK did not have any negative effect on the dose distribution. However, this proposition needs to be supported by making more measurements with different dosimetry methods.

Effect of transcutaneous posterior tibial nerve stimulation and repetitive transcranial magnetic stimulation on neurogenic overactive bladder symptoms in female patients with multiple sclerosis: The study protocol of a randomized controlled study.

Neurogenic bladder is frequently seen in patients with multiple sclerosis (MS). Electrical stimulation methods (neuromodulation) can be used for patients that have persistent symptoms despite pharmacological treatment. This study aims to compare the effects of two different neuromodulation techniques used in the treatment of neurogenic bladder. This is a single-center randomized controlled trial for MS patients with neurogenic bladder. Patients determined to be eligible according to the study criteria will be randomized into two treatment groups: the transcutaneous posterior tibial nerve stimulation (PTNS) and repetitive transcranial magnetic stimulation (rTMS) groups. Each group will include eight patients. The patients will be treated for a total of 10 sessions for two consecutive weeks. The pressure-flow study will be used to compare the initial and final urodynamic results as the primary outcome. All the participants will fill in a 3-day bladder diary before and after the treatments in each group. Patients will also be asked to complete specific questionnaires for incontinence and quality of life (QOL): Overactive Bladder Questionnaire-V8 score (OAB-V8), Incontinence Severity Index (ISI), Incontinence Quality of Life Scale score (I-QOL), International Incontinence Questionnaire (ICIQ-SF) score, and International Consultation on Incontinence Questionnaire-Female Lower Urinary Tract Symptoms (ICIQ-FLUTS) score) as the secondary outcomes. An ethical approval number was obtained from the Non-Invasive Clinical Research Ethics Committee of Istanbul Medipol University (ethical approval number: 768). Support was received within the scope of the Istanbul Medipol University Scientific Research Project with project number 2020-2017. The result of this study will be published in a peer-reviewed journal. NCT05312138.

Magnolia kobus DC.’ nin yeşil çelikle üretiminde farklı hormon uygulamalarının etkileri

Anavatanı Japonya olan Magnolia kobus DC., Magnoliaceae familyasından, kışın yaprağını döken küçük ağaç formunda bir bitkidir. Formu, aromatik yaprakları, bol miktarda açan pembemsi beyaz çiçekleri ve meyve güzelliği ile Peyzaj Mimarlığı bitkilendirme tasarımlarında tercih edilen bitkilerden biridir. Bu çalışmanın amacı, estetik özelikleri ile etkili olan bu bitkinin çelikle çoğaltılarak kentsel peyzajda kullanımının teşvik edilmesidir. Çalışmada, farklı hormon uygulamalarının Magnolia kobus’un yeşil çeliklerinin köklenme başarısı üzerine etkisi araştırılmıştır. Çeliklere, IBA (Indol-3-bütrik asit), IAA (Indol-3-asetik asit) ve NAA (Naftalin asetik asit) hormonların 0 (kontrol), 3000 ppm, 5000 ppm ve 8000 ppm dozları uygulanmıştır. Çelikler sera koşulları altında (20±2 ºC hava sıcaklığı, 25±2 ºC köklendirme masası alt sıcaklığı, %70±2 nem) perlit ortamına dikilmişlerdir. Yaklaşık 4 ay sonra çelikler sökülerek köklenme oranı (%) ve kök uzunlukları (cm) belirlenmiştir. Çalışmanın sonucunda en yüksek köklenme yüzdesi (%60) IBA hormonunun 8000 ppm dozuyla işlem görmüş çeliklerinde elde edilmiştir. Çeliklerdeki en yüksek kök uzunluğu değerleri IAA 3000 ppm ve 5000 ppm dozla işlem görmüş çeliklerde tespit edilmiştir. Yapılan varyans analizi sonucuna göre hormon uygulamalarının köklenme üzerine anlamlı bir etkisi olduğu tespit edilmiştir (P: 0,000 ˂ 0,05).

Silencing of NILCO by Small Interfering RNA Effects MDR1 in Colon Cancer Cells

The aim of the study is to investigate the effect of silencing of NILCO by siRNA on the MDR1 gene, which plays a role in chemoresistance. Cells of the human colon cancer lines Caco‐2 and HT‐29 were cultured at 37˚C in DMEM supplemented with 5% FBS in a humid incubator with 5% CO2. Exponentially growing untreated Caco‐2 and HT‐29 cells were collected and plated (4 x 105/ well in 2 mL) 24 hours before transfection. Plated cells were treated with either double‐stranded siRNA targeting Notch1 mRNA, leptin mRNA, IL‐1 β mRNA, control siRNA, or Paclitaxel (PTX) alone, Notch1 siRNA+PTX, Leptin siRNA+PTX, IL‐1β siRNA+PTX. The siRNAs of Notch1, IL‐1β and Leptin, were applied to the cells at a final concentration of 30 nM, and the cells were transfected with siRNA for 48 hours. After transfection, 100 nM Paclitaxel chemotherapeutic agent for HT‐29 and 130 nM for Caco‐2 was applied to the cells. Cells were harvested for PCR analysis after treatment with PTX for 24 hours.According to our findings, MDR1 gene expression was significantly increased in the paclitaxel group compared to the control group (p&lt;0.05). Silencing of Notch, leptin and IL‐1β decreased MDR1 gene expression compared with the control group, but it was not statistically significant. (p&gt;0.05). However, our results indicated that Notch1 siRNA+PTX, IL‐1β siRNA+PTX and Leptin siRNA+PTX downregulated MDR gene expression in the Caco‐2 cell line compared with PTX alone treated group (p&lt;0.01). In addition, MDR1 gene expression levels were significantly decreased in the Leptin siRNA+PTX and IL‐1β siRNA+PTX groups compared with PTX alone group in the HT‐29 cell line, but the decrease in the Notch1 siRNA+PTX group was not statistically significant. (p&lt;0.01, p&lt;0.05, p&gt;0.05). In conclusion, knockdown of NILCO genes is effective in regulating MDR1 expression, which is a hallmark in chemoresistance.The present study was supported by Eskisehir Osmangazi University Scientific Research Projects (BAP) Coordination Unit (grant no. 202011D11).

Event related delta and theta oscillations are reduced in severe obstructive sleep apnea as a possible electrophysiologial evidence of subtle cognitive changes

AbstractBackgroundObstructive Sleep Apnea Syndrome (OSAS) is a common sleep disorder that leads to widespread cognitive impairment when left untreated. OSAS is also reported as a risk factor for developing Alzheimer’s disease. Recent studies report a shared pathophysiology between OSAS and Alzheimer’s disease (Polsek et al., 2018; Baril et al., 2018). Working memory and attention related ERP P300 deficits were previously reported (for a review see, Raggi and Ferri, 2012). This study aims to investigate electrophysiological changes by using time‐frequency analysis to gain a better understanding on cognitive impairment in OSAS.Method56 severe OSAS patients and age, education and gender matched 33 healthy controls were participated to the study. Participants completed a classical auditory oddball task. A complex Morlet with Gabor normalization was applied and total power within four frequency bandwidths, delta (0.5‐3.5 Hz), theta (4‐7 Hz), alpha (8‐13 Hz), and beta (15‐30 Hz), was calculated using Event‐Related Spectral Perturbation (ERSP) analyses.ResultOSAS patients had reduced delta and theta ERPS to target stimuli compared to healthy controls in repeated measures ANOVA yielding a significant main GROUP effect (respectively, [F(1,87)=9.181, p=0.003] and [F(1,87)=5.083, p=0.027]). No differences were found on alpha and beta power.ConclusionWe found reduced delta and theta activity in severe OSAS patients. Delta EROs activity is associated with attention and decision making (Başar ve Stampfer, 1985; Stampfer ve Başar, 1985), whereas theta activity was associated with task induced activation of working memory (Yordanova et al. 2000). Reduced delta and theta EROs activity can be useful indices of subtle cognitive impairment in severe OSAS even before neuropsychological test. These results strengthen our understanding of the cognitive changes in OSAS by means of electrophysiological methodologies. This study was supported by Dokuz Eylul University Scientific Research Projects (Project Number: 2014.KB.SAG.058).

Development of the Electronic Information System for University Biomedical Engineering Scientific Research Management Based on Computer Internet

The quality of scientific research management (SRM) in universities plays a vital role in the development of universities, and it is still one of the most important factors reflecting university reform and development and informatization construction. As the number of university scientific research (SR) projects has increased year by year and funding has increased, the emergence of diversification has put forward new challenges and requirements for SRM. Therefore, there is an urgent need for university SRM departments. Have it already. It is more systematic, standardized and efficient in managing SR projects. Various evaluation methods of electronic information systems, such as student evaluation, question evaluation, and teacher evaluation, provide a comprehensive view of the university’s medical and bioengineering SRM electronic information system. As for the experimental results, more than 90% of the students in the experimental class accepted interviews and surveys, and more than 90% of the students agreed to research and develop electronic information systems for the management of biomedical engineering SR in the university and expressed their satisfaction. It shows that it is. Based on the computer Internet.

Cerrahi Hastalarının Hemşirelik Bakımını Algılayışı ve Memnuniyet Düzeyleri

Objective: This study was conducted to examine the Nursing Care Perception and Satisfaction Levels of Surgical Patients. Materials and Methods: The sample of this descriptive and cross-sectional study consisted of 300 patients aged 18 and over, who accepted to participate in the study, who were hospitalized at a university hospital surgical clinics between 17 December 2018 and 15 March 2019. Data were collected with the “Patient Information Form” and “Newcastle Nursing Satisfaction Scale” and ”Perception of Nursing Care of Scale” created by the researchers. Percentage, mean, t test, ANOVA, Kruskal Wallis test, Mann-Whitney U test and Pearson’s correlation analysis were used to evaluate the data.This study was supported by Ege University Scientific Research Projects Coordination Unit(Project Number: TLP-2019-20440). Results: The mean satisfaction score of the patients hospitalized in surgical clinics was 79.86 ± 19.31 and the mean score of perception of nursing care was 68.03 ± 9.87. It was found that there was a statistically significant relationship between the total score average of the Newcastle nursing satisfaction scale and the total score average of the perception of nursing care scale (r =, 665; p =, 000). While there was no significant difference between gender, marital status, residence, social security, previous hospital experience and satisfaction with care (p&amp;gt; 0.05), a significant difference was found in terms of education level and chronic disease status, type of hospitalization, number of patients in the room. (p &amp;lt;0.05). Conclusion: Surgical patients were highly satisfied with nursing care, there is a significant relationship between their perception of nursing care and nursing care satisfaction levels, as the educational level of the patients increased, their perception of satisfaction decreased.

Tularemia Seroprevalence in Humans in the Region of the Hittite-Arzawa War (Inner Aegean Region), Where the First Biological Weapon Was Used 3300 Years Ago

Background: According to Egyptian records, tularemia emerged in the Canaan region, where it was first identified and spread to Anatolia over the Euphrates. It was used as an active biological weapon for the first time in the Hittite-Arzawa War in 1320-1318 BC. This study aimed to investigate the seroprevalence of tularemia in the Inner Aegean Region, which is thought to be the regions where this war was fought 3300 years ago. Methods: Tularemia seropositivity in humans was investigated in 27 villages/neighborhoods in 3 districts in each of Manisa, Kutahya, and Usak provinces. Before the study, the participants were informed about the disease via posters, and their blood samples were taken following filling out the questionnaire. Sera were separated from the blood on the same day and stored in a suitable environment until the tests were performed. Microagglutination tests were performed using in-house tularemia antigen and V plate for serological experiments. Rose-Bengal test was also performed on seropositive sera. Results: Of the total 410 people, 226 (55·12%) were male. The mean age of the volunteers was 43·72. Among the age groups of those who volunteered to participate in the study, the most participation was 61 years and over. The most participation was from Kutahya province. According to the results of the tularemia microagglutination test, seropositivity was detected in 6 cases. It was determined that all of the seropositive volunteers were in Kutahya. When the tularemia antibody titers were examined, seropositivity was determined at 1/20-1/160 titers. No positivity was detected in the Rose-Bengal test for cross-reaction. Interpretation: Kutahya has been identified as a risky region in terms of tularemia in the Inner Aegean Region. In order to use the resources in the country economically, first of all, the risk areas in terms of tularemia should be determined by serological studies in all regions. In order to increase awareness about the disease, physicians and filiation teams should be trained in risky areas. Surveillance studies should be conducted to identify and monitor possible sources in areas identified as risky. Funding Information: Trakya University Scientific Research Projects Commission Declaration of Interests: We declare no competing interests. Ethics Approval Statement: This study was carried out with the approval of the Trakya University Scientific Research Ethics Committee dated 13/04/2020 and numbered TUTF-BAEK 2019/381 and the support of the Trakya University Scientific Research Projects Commission (TUBAP) project numbered 2020/121.

Ecclesiastical Personages of Side (Σίδη) of Pamphylia according to Literary and Sphragistic Data

Combining the hagiographic and historiographic data with sigillographic evidence, this article aims to contribute to our understanding of the ecclesiastical personages once active in Side of Pamphylia. It derives from my ongoing work on Byzantine seals, for which I received funding for my on-site and library research project&#x0D; on the “History of Byzantine Side of Pamphylia in the Light of Sigillographic Sources (4th-14th centuries) from the Anadolu University Scientific Research Projects Commission under grant no: E1105E98 (2011). Using old and new sigillographic evidence, duly complemented by relevant references in literary and hagiographic&#x0D; sources, prosopographic lists of the Byzantine officials, courtly and ecclesiastical figures are compiled and presented.

Kynurenine-PARP 1 link mediated by microRNA 210 may be dysregulated in pulmonary hypertension

Abstract Background The aims of the present study were to determine the alterations in mRNA and miR expressions and their role in signaling pathways, to correlate their levels with the severity of PH, and to investigate the relationship between those alterations and serum levels of apelin, kynurenine, and endocan in PH. Methods The study included 32 treatment-naive patients with precapillary PH and 55 controls. All subjects underwent RHC. Total RNA was isolated and cDNAs for mRNA and miR were synthesized. mRNA expressions of HIF-1α, HIF-2α, STAT 3, FGF-2, FGFR-1, PARP 1 and miR expressions of miR-210, miR-130a, miR-424, miR-204, and miR-223 were determined. Concentrations of kynurenine, apelin, and endocan were analyzed. Results HIF-2α, STAT 3, and FGF-2 were increased; miR-210 and miR-130a were increased; miR-223 and miR-204 were decreased. Apelin and kynurenine concentrations were decreased. There were positive correlations: HIF-2α-miR-424: r=0.474, p=0.011; APLN-miR-424: r=0.385, p=0.030; kynurenine-miR-210: r=0.551, p=0.004; STAT 3- PVR: r=0.478, p=0.006; miR-210- RAP: r=0.536, p=0.07; kynurenine-RAP: r=0.409, p=0.022. There were negative correlations: PARP 1-miR-210: r=−0.561, p=0.007; PARP 1- RAP: r=−0.424, p=0.27. miR-130a (OR= 1.257, p=0.016) and APLN (OR= 0.223, p=0.004) were independent risk factors for PH. Conclusions We report: 1) novel relationship between the kynurenine and PARP 1 signaling pathways that could be mediated by miR-210, 2) relationship between the apelin and HIF-2α signaling pathways that could be mediated by miR-424, 3) reduced levels of apelin and elevated levels of miR-130a are associated with PH, 4) elevated levels of STAT 3, miR-210, and kynurenine, and reduced levels of PARP 1 correlate with more severe hemodynamics. Funding Acknowledgement Type of funding source: Other. Main funding source(s): Pamukkale University Scientific Research Projects Coordination Unit

Anadolu Çömlekçiliğinde Gelenek ve İnanışlar

Anadolu topraklarında köklü bir geçmişe sahip olan çömlek üretimi, binlerce yıldır varlık göstermektedir. En eski üretimlerden günümüze kadar gelinen süreçte üretildiği toplumun yeme içme alışkanlıkları, sosyal yapısı, ekonomisi, gelenek, görenek ve inanışları gibi bir çok unsurla o toplumun kültürünü tanımlamaktadır. Bu yönüyle önceleri arkeoloji ve etnoarkeolojinin başlıca çalışma alanını oluşturmaktayken son yıllarda maddi kültür varlıklarının değer görmesiyle sosyal antropologların da ilgi odağı haline gelmiştir. Anadolu’da 1900’lü yılların başından günümüze değin yüzün üzerinde çömlek üretim merkezi bulunmaktadır. Bu merkezlerdeki üretimler ile ilgili bir çok yayın bulunmakta ancak bu yayınlar çoğunlukla toprağın elde edilişinden pişirime kadar geçirdiği süreyi ifade eden teknik anlatımlarla sınırlı kalmaktadır. Oysa her merkezde birbirinden farklılık ve benzerlik gösteren birçok gelenek ve inanış bulunmaktadır. Bu araştırmanın amacı; Anadolu çömlekçiğinin gelenek ve inanışlarla ilgili verilerini sunmak, özellikle ülkemizdeki halkbilimi, folklor, antropoloji ve sosyoloji alanlarında çalışan bilim insanlarının ilgisini çekmek, bu çalışma alanlarına katkı sağlayacağı düşünülen bilgileri paylaşmaktır. Çalışma verilerini; literatür taraması ve 2010-2015 yılları arasında Dokuz Eylül Üniversitesi Bilimsel Araştırma Projesi kapsamında yapılan “Günümüzde Anadolu’da Yaşayan İlkel Çömlekçilik Merkezleri” başlıklı proje oluşturmaktadır. Araştırmada; çömlekçiliğin çıkışı, mesleğin kutsallığı, mesleğin önemi, ürünlerin pişirimi ve ürünlerin kullanım alanları ile ilgili gelenek ve inanışların olduğu saptanmıştır. Bu veriler; Meslek, Pişirim ve Ürünler ile ilgili gelenek ve inanışlar olmak üzere üç ana başlıkta incelenmiştir. Mesleğin rivayetler ile kutsal sayılması, devamlığının sağlanması ve gelecek nesillere aktarılmasında bir çok gelenek ve inanışın olduğu görülmektedir. Üretimin en önemli aşamalarından olan pişirimde deneyimle kazanılan bilgilerin inançlarla bağdaşlaştırılarak ritüellere dönüştüğü, ayrıca üretilen ürünlerin, toplumun sosyal yaşam dinamiklerinden olan düğün ve ölüm olaylarında bir ifade aracı olarak kullanıldığı görülmektedir.

The Role of Mitochondria in Cyanotic and Acyanotic Congenital Heart Disease

Objectives: Congenital heart disease (CHD) is one of the most specialized field of heart surgery and compelling approaches such as mitochondrial transplantation is creating promising options. Therapeutic options are available, but lack of data on mitochondrial function in this disease group. Hence, we hypothesized that there is a differential level mitochondrial dysfunction in cyanotic vs acyanotic CHD groups and aimed to indicate any potential pediatric patient group that is suitable for this innovating therapeutic approach. Hence, in this study, mitochondrial morphology and function, myocardial fibrosis, and myocyte atypia were evaluated in myocardial tissue of pediatric patients with cyanotic and acyanotic CHD. Additionally, cardiac enzymes have been reviewed as a potential indicator for postoperative follow-up. Methods: 5 acyanotic CHD and 5 cyanotic CHD patients were included according to clinical parameters of patients. During the open heart surgery, myocardial biopsies (2–3 mm³) were obtained from right atrium. Mitochondrial morphology was evaluated by immunofluorescence staining using anti-Tom20 antibody, electron transport chain complexes of myocardium were examined by histological cytochrome c oxidase/succinate dehydrogenase (COX/SDH) histochemical staining and ATP production was measured. Hematoxylin and eosin, and Masson ’ s trichrome staining were used to determine histopathological changes. In both preoperative and postoperative period, cardiac enzymes of each patient were monitored. Myocyte atypia, and fibrosis were classified on a scale of 1 to 4. All data were analyzed to determine any correlation between all parameters. Results: In the research study, unlike patients with acyanotic CHD, alterations in mitochondrial network and reduction in ATP production were detected in all pediatric patients with cyanotic CHD. These findings may be assumed as an evidence for the relationship between mitochondrial dysfunction and cyanotic CHD. As well as, statistically significant correlation was determined between mitochondrial dysfunction and cardiac enzyme levels. Conclusion: Due to the fact that mitochondrial in myocardium of pediatric patient group with cyanotic CHD were determined, mitochondrial transplantation may be considered as a futuristic therapeutic approach to increase the surgical success in patients with cyanotic CHD. Funding Statement: This work was supported by Hacettepe University Scientific Research Projects Coordination Unit, Project Number: THD-2018-16687 Declaration of Interests: There is no conflict of interest. Ethics Approval Statement: This study protocol was approved by Hacettepe University Faculty of Medicine Ethical Review Board (GO 17/651). Additionally, on behalf of all named authors, I, the Corresponding Author, declare that all experiments were performed in accordance with guidelines, and regulations of Declaration of Helsinki.