To explore Tuberculosis (TB) health promotion and education in universities, and to provide a feasible reference method and specific implementation measures for improving health education among university students. We collected a total of 811 respondents, using a uniform questionnaire online survey questionnaire network. The completed questionnaires were double-entered using EpiData3.1, the database was established, and the analysis was performed by Excel2016 and SPSS 22 software. The total awareness rate of the 8 core information on tuberculosis prevention and control was 74.2 %. Among them, “the state provides free anti-tuberculosis drugs and major tests for infectious tuberculosis patients” with the lowest awareness rate of 49.2 %; followed by the awareness rate of “should care about tuberculosis patients and should not discriminate against tuberculosis patients”, for 63.8 %. The channels for college students to acquire knowledge about tuberculosis prevention were “newspapers and magazines”, accounted for 50.3 percent; “broadcasting, television and video” accounted for 52.4 %; “wall advertising, bulletin boards, slogans” accounted for 44.6 %; “School Health Education” accounted for 38.5 % of “school propaganda columns or publicity panels” accounted for 34.9 %. The favourite way for college students to promote was “watching TV” 40.3 %.