Ultrasound Transducer Array Research Articles

Electrowetting-driven liquid lens for ultrasound: Enabling controllable focal length and flexible beam steering

Focused ultrasound is an increasingly popular non-invasive treatment modality. Still, its fixed focal point requires an array ultrasound transducer or scanning system to cover different therapeutic scenarios. To address this limitation, we developed an electrically-controlled liquid lens that enables dynamic beam focusing and steering of the incident plane ultrasound beam. The lens was carefully optimized for low-energy attenuation and low-voltage driving. We evaluated the performance of the lens using a homemade 5.5-MHz planar transducer with a 7.5-mm aperture. Our results demonstrate that the planar ultrasound beam can be adjusted to a focused beam with a focal length from 27 mm to 32 mm within 1 s by increasing the electric input (0–60 V) to the lens. Additionally, the beam angle of the ultrasound is tunable from −5 to 5° by adjusting the charge distribution on the lens. Our design enables real-time, fast-response, on-demand changing of focal length and beam angle for a single-element planar transducer. Our study presents a promising technology for altering the ultrasound beam of a planar single-element transducer for different ultrasound applications. The development of this electrically-controlled liquid lens has the potential to enhance the efficacy of focused ultrasound treatment and improve patient outcomes.

A 2D Ultrasound Phased-Array Transmitter ASIC for High-Frequency US Stimulation and Powering.

Ultrasound (US) neuromodulation and ultrasonic power transfer to implanted devices demand novel ultrasound transmitters capable of steering focused ultrasound waves in 3D with high spatial resolution and US pressure, while having a miniaturized form factor. Meeting these requirements needs a 2D array of ultrasound transducers directly integrated with a high-frequency 2D phased-array ASIC. However, this imposes severe challenges on the design of the ASIC. In order to avoid the generation of grating lobes, the elements in the 2D phased-array should have a pitch of half of the ultrasound wavelength, which, as frequency increases, highly reduces the area available for the design of high-voltage beamforming channels. This article addresses these challenges by presenting the system-level optimization and implementation of a high-frequency 2D phased-array ASIC. The system-level study focuses on the optimization of the US transmitter toward high-frequency operation while minimizing power consumption. This study resulted in the implementation of two ASICs in TSMC 180 nm BCD technology: firstly, an individual beamforming channel was designed to demonstrate the tradeoffs between frequency, driving voltage, and beamforming capabilities. Finally, a 12-MHz pitch matched 12 × 12 phased-array ASIC working at 20-V amplitude and 3-bit phasing was designed and experimentally validated, to demonstrate high-frequency phased-array operation. The measurement results verify the phasing functionality of the ASIC with a maximum DNL of 0.35 LSB. The CMOS chip consumes 130 mW and 26.6 mW average power during the continuous pulsing and delivering 200-pulse bursts with a PRF of 1 kHz, respectively.

Single-channel acoustic vortex tweezer with attachable fan-shaped holographic lens

The utilization of acoustic vortices presents a promising technique for contactless particle manipulation. However, existing methods for generating acoustic vortices typically rely on complex active driving systems and array ultrasound transducers. Therefore, more simplified and robust approaches to inducing acoustic vortices should be considered. With inspiring the fact that acoustic vortices can be generated by the differences in the height of adjacent sectors in conventional four-panel transducers, we aim to develop a single-channel acoustic vortex tweezer with a simplified driving system by incorporating attachable fan-shaped polydimethylsiloxane (PDMS) holographic lens. This passive lens structure with different heights in adjacent sectors can generate phase-modulated ultrasonic waves. Therefore, the interaction of phase-modulated waves passing through the lens is expected to result in a vortex-like beam. The available materials of fan-shaped lens are not limited to the PDMS. This lens type can be appropriately fabricated with an understanding of the sound of speed within the desired material and predetermined thickness, inducing π/2 phase difference in the adjacent lobes. As a result, the excited acoustic waves propagated through the lens were under mutual interaction without any active electrical setup, forming a null pressure region enveloped by four distinct acoustic lobes. We systemically investigated the tweezing potential using vortex-like waves. Several types of bioparticles were utilized in these investigations: microbubbles, polystyrene (PS) beads, and cancer cells. Our findings demonstrate that the transducer with lens is sufficiently capable of spatial manipulation of bioparticles. Remarkably, the microbubbles traveling at a flow velocity of 32 cm/s in a blood vessel-mimicking phantom were feasibly trapped in the proposed transducer. Furthermore, the spatial control of PS and cells with higher acoustic impedance was also feasible in static conditions. Consequently, the proposed transducer offers an economically and practically viable solution for various biomedical applications, such as drug delivery and cell-based bioengineering.

Open-UST: An Open-Source Ultrasound Tomography Transducer Array System.

Fast imaging methods are needed to promote clinical adoption of ultrasound tomography (UST), and more widely available UST hardware could support the experimental validation of new measurement configurations. In this work, an open-source 256-element transducer ring array was developed (morganjroberts.github. io/open-UST) and manufactured using rapid prototyping, for only £2k. Novel manufacturing techniques were used, resulting in a 1.17° mean beam axis skew angle, a [Formula: see text] mean element position error, and a [Formula: see text] deviation in matching layer thickness. The nominal acoustic performance was measured using hydrophone scans and watershot data, and the 61.2 dB signal-to-noise ratio (SNR), 55.4° opening angle, 10.2 mm beamwidth, and 54% transmit-receive bandwidth (-12 dB) were found to be similar to existing systems and compatible with state-of-the-art full-waveform-inversion image reconstruction methods. The interelement variation in acoustic performance was typically < 10% without using normalization, meaning that the elements can be modeled identically during image reconstruction, removing the need for individual source definitions based on hydrophone measurements. Finally, data from a phantom experiment were successfully reconstructed. These results demonstrate that the open-UST system is accessible for users and is suitable for UST imaging research.

Temperature-Controlled Hyperthermia with Non-Invasive Temperature Monitoring through Speed of Sound Imaging

Hyperthermia therapy (HT) is used to treat diseases through heating of high temperature usually in conjunction with some other medical therapeutics such as chemotherapy and radiotherapy. In this study, we propose a promising temperature-controlled hyperthermia method that uses high-intensity focused ultrasound (HIFU) for clinical tumor treatment combined with diagnostic ultrasound image guidance and non-invasive temperature monitoring through speed of sound (SOS) imaging. HIFU heating is realized by a ring ultrasound transducer array with 256 elements. In this study, tumors in the human thigh were set as heating targets. The inner structure information of thigh tissue is obtained by B-mode ultrasound imaging. Since the relationship between temperature and SOS in different human tissue is available, the temperature detection is converted to the SOS detection obtained by the full-wave inversion (FWI) method. Simulation results show that our model can achieve expected hyperthermia of constant temperature on tumor target with 0.2 °C maximum temperature fluctuation for 5 h. Through simulation, our proposed thermal therapy model achieves accurate temperature control of ±0.2 °C in human thigh tumors, which verifies the feasibility of the proposed temperature-controlled hyperthermia model. Furthermore, the temperature measurement can share the same ring ultrasound transducer array for HIFU heating and B-mode ultrasound imaging, which provides a guiding significance for clinical application.

A transceiver integrated piezoelectric micromachined ultrasound transducer array for underwater imaging

In this article, a scandium-doped aluminum nitride (Sc0.2Al0.8N) piezoelectric micromachined ultrasound transducer (PMUT) array for underwater imaging is presented. The reported PMUT array integrates a transmitter and a receiver in one transduction cell, in which the outer top electrode is used for transmitting while the inner top electrode is employed for simultaneously receiving ultrasonic waves, respectively. A 7 × 8 transduction cell array with characteristic size of 500μm is designed in a bioinspired honeycomb architecture and developed based on a piezoelectric on silicon-on-insulator (SOI) platform. The top electrode of the transduction cell is split into the outer and inner sections with the optimized size ratio for achieving transceiver integration in one cell. The fabricated PMUT array exhibits a transmit sensitivity of 146.59 dB (re: 1 μPa/V), and a receive sensitivity of −173.70 dB (re: 1 V/μPa) at its resonant frequency of around 150 kHz in water. In order to demonstrate the feasibility of underwater imaging, the PMUT array and its signal conditional circuits are packaged using an acoustically transparent material. Ultrasonic pulse-echo imaging with the developed PMUT array is demonstrated without implementation of a switching circuit to separate the transmit and reflected signals, due to the transceiver integration approach proposed in our work. This work demonstrates the feasibility of underwater imaging for the transceiver integrated PMUT array, which has great commercial value for minimized and portable underwater imaging.

A method for the geometric calibration of ultrasound transducer arrays with arbitrary geometries

Geometric calibration of ultrasound transducer arrays is critical to optimizing the performance of photoacoustic computed tomography (PACT) systems. We present a geometric calibration method that is applicable to a wide range of PACT systems. We obtain the speed of sound and point source locations using surrogate methods, which results in a linear problem in the transducer coordinates. We characterize the estimation error, which informs our choice of the point source arrangement. We demonstrate our method in a three-dimensional PACT system and show that our method improves the contrast-to-noise ratio, the size, and the spread of point source reconstructions by 80±19%, 19±3%, and 7±1%, respectively. We reconstruct the images of a healthy human breast before and after calibration and find that the calibrated image reveals vasculatures that were previously invisible. Our work introduces a method for geometric calibration in PACT and paves the way for improving PACT image quality.

A Pitch-Matched Low-Noise Analog Front-End With Accurate Continuous Time-Gain Compensation for High-Density Ultrasound Transducer Arrays

This article presents a compact analog front-end (AFE) circuit for ultrasound receivers with linear-in-dB continuous gain control for time-gain compensation (TGC). The AFE consists of two variable-gain stages, both of which employ a novel complementary current-steering network (CCSN) as the interpolator to realize continuously variable gain. The first stage is a trans-impedance amplifier (TIA) with a hardware-sharing inverter-based input stage to save power and area. The TIA’s output couples capacitively to the second stage, which is a class-AB current amplifier (CA). The AFE is integrated into an application-specific integrated circuit (ASIC) in a 180-nm high-voltage BCD technology and assembled with a 100 -pitch PZT transducer array of 8 <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$\times$</tex-math> </inline-formula> 8 elements. Both electrical and acoustic measurements show that the AFE achieves a linear-in-dB gain error below <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$\pm$</tex-math> </inline-formula> 0.4 dB within a 36-dB gain range, which is <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$&gt;$</tex-math> </inline-formula> 2 <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$\times$</tex-math> </inline-formula> better than the prior art. Per channel, the AFE occupies 0.025 area, consumes 0.8 mW power, and achieves an input-referred noise density of 1.31 .

Airborne ultrasound focusing aperture with binary amplitude mask over planar ultrasound emissions

Phased arrays of airborne ultrasound transducers are widely utilized as a key technology to achieve mid-air convergence of intense ultrasound, which is applied to a variety of systems, such as contactless tactile presentation, acoustic levitation and its application, mid-air-flow acceleration, etc. However, it requires considerably precise phase control with temporally severe synchronization between elements, which leads to difficulty in scaling up the entire system beyond the tabletop size as most of the current application systems. Here, we propose a much simpler and easier scaling-up method of airborne ultrasound convergence, where a binary amplitude mask that serves as a Fresnel Zone Plate (FZP) is placed on the planar in-phase ultrasound sources. We experimentally demonstrate that the FZP-based ultrasound focusing achieved a spatial resolution that is comparable to conventional methods, based on the use of phase-controlled transducers. The ultrasound foci created using FZPs are sufficiently intense for most application scenarios that are currently in practical use. We also determine favorable side effects of our method suppressing grating lobes, which is inevitable with the conventional phase-controlling method. The FZPs and planar ultrasound sources are both readily implemented with inexpensive ingredients and components. The result of our study contributes to upsizing dimensions in which a mid-air convergent ultrasound field is successfully generated. Accordingly, unprecedented application scenarios that target the entire room as the workspace will be possible.

MP23-05 NONINVASIVE ACOUSTIC FORCEPS USING ULTRASOUND TO MANIPULATE OBJECTS IN THE URINARY TRACT

You have accessJournal of UrologyCME1 Apr 2023MP23-05 NONINVASIVE ACOUSTIC FORCEPS USING ULTRASOUND TO MANIPULATE OBJECTS IN THE URINARY TRACT Mohamed Ghanem, Arturo Holmes, Adam Maxwell, Michael Bailey, Mathew Sorensen, and Jonathan Harper Mohamed GhanemMohamed Ghanem More articles by this author , Arturo HolmesArturo Holmes More articles by this author , Adam MaxwellAdam Maxwell More articles by this author , Michael BaileyMichael Bailey More articles by this author , Mathew SorensenMathew Sorensen More articles by this author , and Jonathan HarperJonathan Harper More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000003248.05AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Ultrasonic propulsion has been demonstrated in clinical trials to noninvasively reposition renal stones along the direction of the probe. However, extracorporeal manipulation of stones causing ureteral stone obstruction requires controlled movement along a complex path in the urinary tract. Here, we evaluate the safety and utility of a method for controlled manipulation of stone models in vivo in a complex three-dimensional (3D) path using focused ultrasound beams. METHODS: A multi-element ultrasound array transducer with a 12-cm focus was used to produce an acoustic trap around a stone model (3 mm glass sphere) in the bladder of three pigs under anesthesia. The array was coupled to the pig by a water bath and synchronized with a P6-3 ultrasound imaging probe for targeting. A cystoscope was inserted into the bladder to visualize the motion. The sphere was acoustically trapped and steered along three different paths (Figure 1a-c). Deviation of the measured from the intended path was calculated to quantify the accuracy. The total energy exposure to manipulate the stone was low; at 1.5 MHz, the maximum exposure had a maximum peak pressure of 1.4 MPa and a pulse average intensity of 133 W/cm2. The bladder was then evaluated histologically. RESULTS: Spheres were levitated and moved in the three paths in all pigs for a total of 26 manipulations. The spheres were lifted and moved 1) laterally 3 mm and back down, 2) in a horizontal, and 3) in a vertical loop of 3 mm radius. The average discrepancy between the desired path and that observed on ultrasound was 0.24±0.07 mm, and by the cystoscope was 0.17±0.09 mm. The sphere was dragged for 5 cm along the bladder wall by moving the array mechanically (Figure 1d). The skin to stone depth was 2±0.5 cm. Histology showed no signs of injury to the bladder wall. CONCLUSIONS: This work demonstrated the controlled, noninvasive manipulation of stone models in vivo at safe ultrasound power levels. This work potentiates the use of noninvasive ultrasound tweezers to facilitate movement of stones in any direction which could expand the capabilities of ultrasonic propulsion alone. Source of Funding: Work supported by NIDDK P01 DK043881, K25 DK132416 © 2023 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 209Issue Supplement 4April 2023Page: e307 Advertisement Copyright & Permissions© 2023 by American Urological Association Education and Research, Inc.MetricsAuthor Information Mohamed Ghanem More articles by this author Arturo Holmes More articles by this author Adam Maxwell More articles by this author Michael Bailey More articles by this author Mathew Sorensen More articles by this author Jonathan Harper More articles by this author Expand All Advertisement PDF downloadLoading ...

Biofouling Removal Using a Novel Electronic System for Driving an Array of High Power Marinised Transducers

This paper explains producing a novel ultrasonic system to remove/prevent biofouling growth from wind turbines’ access ladders by means of producing local ultrasound cavitation. Using bespoke hardware, an array of high-power ultrasound transducers (HPUTS) and optimally synthesized signal types to remove/prevent biofouling growth from the ladder without violating the standard noise level in the sea is explained. This is a non-toxic and non-invasive solution to detach biofouling and prevent biofilm initiation on offshore structures. It is shown that the marinisation of the HPUT slightly shifts the main resonance frequency from 28.1 to 27.5 kHz. The vibration output from the HPUTs with different mounting systems showed that the transducer with the horn could vibrate the plate at 20 cm from the excitation point, with 300 pm, six times higher than the vibration output from the marinised HPUT. A transducer array and attachment are proposed to make the ultrasound noise below the standard underwater noise limits. The produced sound pressure level (SPL) and sound equivalent level (SEL) from the proposed ultrasonic system was measured. It was specified that the SPL came below 120 dB at 25 m from the excitation point and the SEL value below the 173 dB limit. Finally, the effectiveness of the marinised HPUTS on biofouling removal has been demonstrated with an in-situ measurement, and it was indicated that local biofouling removal could be achieved.

Super-resolution cellular ultrasound imaging via localization of nanodroplets

Ultrasound localization microscopy (ULM) had made it possible to differentiate microbubble contrast agents that are separated only by a few micrometers and, thus, opening the path for super-resolution imaging. However, due to the innate limits of acoustic waves and microbubble imaging, similar levels of resolution to optical imaging (i.e., submicron resolution) are still very challenging. In this paper, we utilize ULM to achieve cellular imaging by using phase-change perfluorocarbon nanodroplets (PFCnDs) as a contrast agent that were fabricated with spontaneous nucleation method. To identify the point spread function (PSF) of a single nanodroplet, gaussian fit function was applied for reconstruction. Nanodroplets were injected into cells through patch clamping and later captured with two transducers: a single element transducer for focused ultrasound and linear array transducer for imaging. Nonlinear imaging (NLO) was used to maximize the sound to noise ratio (SNR), which enables optimal amplitude. With the PSF of a phase-transitioned nanodroplet known, stochastic activation of multiple nanodroplets within a cell was accumulated to image a full cell morphology. These findings can lead researchers to develop effective ultrasound imaging paradigms that can visualize intracellular level of organs in deep tissue invivo.

Aluminum Nitride Piezoelectric Micromachined Ultrasound Transducer Arrays for Non-Invasive Monitoring of Radial Artery Stiffness

An aluminum nitride (AlN) piezoelectric micromachined ultrasound transducer (PMUT) array was proposed and fabricated for non-invasive radial artery stiffness monitoring, which could be employed in human vascular health monitoring applications. Using surface micromachining techniques, four hexagonal PMUT arrays were fabricated within a chip area of 3 × 3 mm2. The mechanical displacement sensitivity and quality factor of a single PMUT were tested and found to be 24.47 nm/V at 5.94 MHz and 278 (in air), respectively. Underwater pulse-echo tests for the array demonstrated a -3 dB bandwidth of 0.76 MHz at 3.75 MHz and distance detection limit of approximately 25 mm. Using the PMUT array as an ultrasonic probe, the depth and diameter changes over cardiac cycles of the radial artery were measured to be approximately 3.8 mm and 0.23 mm, respectively. Combined with blood pressure calibration, the biomechanical parameters of the radial artery vessel were extracted using a one-dimensional vascular model. The cross-sectional distensibility, compliance, and stiffness index were determined to be 4.03 × 10-3/mmHg, 1.87 × 10-2 mm2/mmHg, and 5.25, respectively, consistent with the newest medical research. The continuous beat-to-beat blood pressure was also estimated using this model. This work demonstrated the potential of miniaturized PMUT devices for human vascular medical ultrasound applications.

Integration of microlenses on surface-micromachined optical ultrasound transducer array to improve detection sensitivity for parallel data readout.

This Letter reports the integration of microlenses (MLs) on a surface-micromachined optical ultrasound transducer (SMOUT) array to enable parallel ultrasound data readout from a multiplicity of elements. The MLs are fabricated by photoresist patterning and reflow, and their focal lengths are optimized with parametric studies. Experiments are conducted to characterize the acoustic responsivity and its uniformity of the SMOUT-ML elements under different conditions. The temporal stability of SMOUT-ML elements immersed in water is assessed by monitoring their acoustic response continuously for 1 week. Parallel ultrasound signal readout is simulated with a small group of SMOUT-ML elements. Experimental results show that high acoustic sensitivity and excellent long-term stability can be achieved by the ML-integrated SMOUT array, which could provide a promising approach for enabling parallel ultrasound data acquisition for improving the imaging speed of 3D acoustic tomography.

High-fidelity deep functional photoacoustic tomography enhanced by virtual point sources

Photoacoustic tomography (PAT), a hybrid imaging modality that acoustically detects the optical absorption contrast, is a promising technology for imaging hemodynamic functions in deep tissues far beyond the traditional optical microscopy. However, the most clinically compatible PAT often suffers from the poor image fidelity, mostly due to the limited detection view of the linear ultrasound transducer array. PAT can be improved by employing highly-absorbing contrast agents such as droplets and nanoparticles, which, however, have low clinical translation potential due to safety concerns and regulatory hurdles imposed by these agents. In this work, we have developed a new methodology that can fundamentally improve PAT’s image fidelity without hampering any of its functional capability or clinical translation potential. By using clinically-approved microbubbles as virtual point sources that strongly and isotropically scatter the local pressure waves generated by surrounding hemoglobin, we can overcome the limited-detection-view problem and achieve high-fidelity functional PAT in deep tissues, a technology referred to as virtual-point-source PAT (VPS-PAT). We have thoroughly investigated the working principle of VPS-PAT by numerical simulations and in vitro phantom experiments, clearly showing the signal origin of VPSs and the resultant superior image fidelity over traditional PAT. We have also demonstrated in vivo applications of VPT-PAT for functional small-animal studies with physiological challenges. We expect that VPS-PAT can find broad applications in biomedical research and accelerated translation to clinical impact.

A Novel Concept of Transperineal Focused Ultrasound Transducer for Prostate Cancer Local Deep Hyperthermia Treatments

Design, embodiment, and experimental study of a novel concept of extracorporeal phased array ultrasound transducer for prostate cancer regional deep hyperthermia treatments using a transperineal acoustic window is presented. An optimized design of hyperthermia applicator was derived from a modelling software where acoustic and thermal fields were computed based on anatomical data. Performance tests have been experimentally conducted on gel phantoms and tissues, under 3T MRI guidance using PRFS thermometry. Feedback controlled hyperthermia (ΔT = 5 °C during 20min) was performed on two ex vivo lamb carcasses with prostate mimicking pelvic tissue, to demonstrate capability of spatio-temporal temperature control and to assess potential risks and side effects. Our optimization approach yielded a therapeutic ultrasound transducer consisting of 192 elements of variable shape and surface, pseudo randomly distributed on 6 columns, using a frequency of 700 kHz. Radius of curvature was 140 mm and active water circulation was included for cooling. The measured focusing capabilities covered a volume of 24 × 50 × 60 mm3. Acoustic coupling of excellent quality was achieved. No interference was detected between sonication and MR acquisitions. On ex vivo experiments the target temperature elevation of 5 °C was reached after 5 min and maintained during another 15 min with the predictive temperature controller showing 0.2 °C accuracy. No significant temperature rise was observed on skin and bonny structures. Reported results represent a promising step toward the implementation of transperineal ultrasound hyperthermia in a pilot study of reirradiation in prostate cancer patients.

Quantitative Evaluation of In Vivo Corneal Biomechanical Properties after SMILE and FLEx Surgery by Acoustic Radiation Force Optical Coherence Elastography.

The purpose of this study is to quantitatively evaluate the differences in corneal biomechanics after SMILE and FLEx surgery using an acoustic radiation force optical coherence elastography system (ARF-OCE) and to analyze the effect of the corneal cap on the integrity of corneal biomechanical properties. A custom ring array ultrasound transducer is used to excite corneal tissue to produce Lamb waves. Depth-resolved elastic modulus images of the in vivo cornea after refractive surgery were obtained based on the phase velocity of the Lamb wave. After refractive surgery, the average elastic modulus of the corneal flap decreased (71.7 ± 24.6 kPa), while the elastic modulus of the corneal cap increased (219.5 ± 54.9 kPa). The average elastic modulus of residual stromal bed (RSB) was increased after surgery, and the value after FLEx (305.8 ± 48.5 kPa) was significantly higher than that of SMILE (221.3 ± 43.2 kPa). Compared with FLEx, SMILE preserved most of the anterior stroma with less change in corneal biomechanics, which indicated that SMILE has an advantage in preserving the integrity of the corneal biomechanical properties. Therefore, the biomechanical properties of the cornea obtained by the ARF-OCE system may be one of the essential indicators for evaluating the safety of refractive surgery.

Sequential Dynamic Aperture Focusing Strategy for Transmissive Ultrasonic Phase Array Tomography

Industrial ultrasonic tomography (UT) possesses unique benefits in nonintrusive detection of cross-sectional medium and has received broad attention in decades. Aiming at the complex bubble imaging, the conventional independent-transducer-based UT has low spatial resolution due to wide projection path and low projection number. Based on ultrasound phase array (UPA) transducers, this article proposes a sequential dynamic aperture focusing strategy for transmissive ultrasonic phase array tomography (UPAT). The emitting aperture focuses the ultrasound beam on a close distance to generate a high intensity and wide-angle acoustic field that covers multiple receivers in single excitation and further induces high amount of multiangle narrow projections, through which the measurement speed and imaging performance are substantially improved. Beyond numerical analysis on image accuracy and noise robustness, an experimental UPAT system with independent T/R channels is developed to evaluate the proposed method, where the improvement on imaging accuracy and speed are testified through qualitatively and quantitatively comparison with the state-of-art UT and the beam scanning UPAT techniques. The UPAT-focus strategy is proved to be advanced and feasible in imaging complex medium distribution.

Optoacoustic imaging of the glymphatic system

Background: The glymphatic system is a brain waste clearance system mediated via cerebrospinal fluid (CSF) flow,1 with implications for influence on neurodegenerative diseases.2 Most preclinical glymphatic studies employ fluorescence imaging, which provides higher specificity, but a smaller field-of-view (FOV), or magnetic resonance imaging (MRI), which provides brain-wide FOV, but lower specificity. Functional optoacoustic neuro-tomography3 (FONT) offers a larger FOV compared to classical optical methods, and higher specificity compared to MRI. However, FONT has not yet been applied to probe the glymphatic system. In this study, we used fluorescence and optoacoustic imaging of a near-infrared dye, Janelia Fluor 669 (JF669), to track CSF and multimodal CSF-hemodynamic flows in mice.&#x0D; Methods: After observing strong fluorescence and optoacoustic signatures of JF669 in phantom experiments, we performed a series of in vivo experiments in isoflurane-anesthetized C57BL/6 mice (n=3 fluorescence and n=4 FONT experiments, respectively) (Figure 1A). The lumbar injection was applied to deliver JF669 at a rate of 2 µL/min for 30 minutes. A polyethylene tube was placed intrathecally at the lumbar region (L4-L5). The scalp was removed. Fluorescence or FONT images were obtained every 5 minutes after injection.&#x0D; Results: Fluorescence imaging and FONT probe CSF flow Images (Figure 1B) and time traces (Figure 1C) revealed time-dependent anatomical routes of paravascular influx, including the transport along the olfactory artery (OFA), superior cerebellar artery (SCA), and bilateral middle cerebral artery (MCA). For FONT imaging, since the OFA showed strong fluorescence (Figure 1B), we positioned the ultrasound transducer array at the anterior of the mouse brain with a FOV of 5x5 mm2 (Figure 1A). Standard filtered back-projection reconstruction was applied. Besides the OFA route of the paravascular influx, the dynamic images (Figure 1D) and time-traces (Figure 1E) also revealed time-dependent anatomical routes of CSF-interstitial fluid (ISF) exchange in the olfactory bulb (OFB) and paravascular efflux in the superior sagittal sinus (SSS) and the bilateral inferior cerebral vein (ICV). Next, we studied the aquaporin-4 (AQP4) dependence of glymphatic flow by subcutaneously injecting AQP4 inhibitor TGN020, in addition to the prior procedures. Under fluorescence imaging and FONT, we observed that TGN020 significantly decreased and spatially restricted the spread of JF669 in the brain. FONT spectral unmixing separates CSF and blood We swept the OPO through 680 nm to 750 nm, with 10 nm steps at 10 Hz in the phantom and in each animal. The multispectral reconstructions were unmixed using the known absorption spectra of hemoglobin and the JF669 OA spectrum obtained from the phantom.4 This enabled the separation of blood and JF669 signal (Figure 1D).&#x0D; Conclusions: We characterized anatomical routes of paravascular influx (OFA), CSF-ISF exchange (OFB) and paravascular efflux using FONT, while fluorescence imaging only enabled visualization of the paravascular influx. We thus demonstrated the feasibility and advantages of FONT for probing the glymphatic system. These findings present great opportunities for monitoring and understanding the glymphatic system during development, aging, disease, pharmacological interventions and genetic modifications.

Beyond fundamental resonance mode: high-order multi-band ALN PMUT for in vivo photoacoustic imaging

This paper reports on an aluminum nitride (AlN) piezoelectric micromachined ultrasound transducer (PMUT) array for photoacoustic (PA) imaging, where the high-order resonance modes of the PMUT are utilized to improve imaging resolution. A flexural vibration mode (FVM) PMUT is fabricated and applied in a photoacoustic imaging (PAI) system. Specifically, the microelectromechanical system (MEMS)-based PMUT is suitable for PA endoscopic imaging of blood vessels and bronchi due to its miniature size and high sensitivity. More importantly, AlN is a nontoxic material, which makes it harmless for biomedical applications. In the PAI system, the AlN PMUT array is used to detect PA signals, and the acousto–mechanical response is designed and optimized at the PMUT’s fundamental resonance. In this work, we focus on the high-order resonance performance of the PMUT PAI beyond the fundamental resonance. The acoustic and electrical responses of the PMUT’s high-order resonance modes are characterized and analyzed. The fundamental and three high-order resonance bandwidths are 2.2, 8.8, 18.5, and 48.2 kHz. Compared with the resolution at the fundamental resonance mode, the resolutions at third- and fourth-order resonance modes increase by 38.7% and 76.9% in a phantom experiment. The high-order resonance modes of the AlN PMUT sensor array provide higher central frequency and wider bandwidth for PA signal detection, which increase the resolution of PAI compared to the PMUT working at the fundamental resonance mode.