Tyrrhenian Coast Research Articles

Late Holocene human-induced landscape changes in Calcareous Tufa environments in Central Mediterranean valleys (Pecora river, Southern Tuscany, Italy)

Two stages of high-discharge fluvial deposition during the last phase of the Late Holocene are recorded in the infilling of a palaeochannel in the distal reach of the Pecora river, a small catchment along the Tyrrhenian coast of Tuscany (Central Italy). Facies analysis shows an abrupt change from high sinuosity gravelly-sandy river (III-I millennium BC) to shallow braided channel bars (VII-XII century AD). The second stage features the occurrence of very abundant sediments originated from the erosion of the upstream Calcareous Tufa environments, coupled with abundant fine- to coarse-sized charcoal fragments, derived by selective fires sequentially affecting the wetlands, the riparian vegetation, and the surrounding slopes. Geomorphological and stratigraphical analysis of the Pecora river catchment indicates that the widespread wetlands in which Calcareous Tufa were developing were already active around 4 ka cal BC and their formation ended permanently around the XIII century AD. The age of the decline of the tufa systems in the Pecora catchment seems to disagree with most of those recorded in Europe. Contrastingly, their inactivity is coeval with the occupational period of the medieval archaeological site of Vetricella, located in the surroundings of the palaeochannel, and the onset of upstream wetland reclamation and hydraulic works. These changes led to the reclamation of karst depressions, the capture of karst springs, the bypass of barrages and the deep downcutting of the tufa terraces and the drainage of the corresponding wet environments. This study discusses the anthropogenic factors that during the Early Middle Age affected the Pecora catchment and promoted a permanent change in its physical and biological environment with a new assessment of the river course and the reclamation of large portions of floodplains previously permanently flooded. This study provides new data regarding the decline of fluvial tufa in the Mediterranean area and emphasises the Early Medieval anthropogenic forcing mechanism tethered to agricultural practices and land management, contrasting with the classic historical model which suggests an almost complete abandonment of the alluvial and coastal plains.

Far from home: Genetic variability of Knipowitschia sp. from Italy revealed unexpected species in coastal lagoons of the Tyrrhenian coast

Coastal lagoons are resilient and productive ecosystems that support high biological and habitat diversity, but are increasingly affected by several threats due to human exploitation. Many resident species of these environments show a restricted geographical range and a strict association with specific habitats, thus they could represent ecological indicators of these ecosystems. Data on the genetic variability distribution in populations of these species are crucial to identify the presence of different evolutionary units across their geographical distribution ranges and to plan actions for their management and conservation. In this context, the assessment of the genetic variability and structure of Italian specimens of the Adriatic dwarf goby Knipowitschia panizzae, a brackish species endemic in the Adriatic Sea, and included among the species of Community interest in Annex II of Habitats Directive, was carried out. To this purpose, goby samples were collected both from Italian sites where the species is native (Adriatic) and non-native (Tyrrhenian), probably introduced due to the practice of stocking lakes and coastal lagoons with juvenile of euryhaline species to sustain local fisheries. Results show the presence of high values of haplotype diversity, and no shared haplotypes between fish from sites where the species is native or introduced. Moreover, in Tyrrhenian Italian lagoons we identified an allochthonous species of Knipowitschia, previously undetected in Italy: the Corfu dwarf goby K. goerneri. This species was originally described as endemic to the Korission Lagoon catchment on Corfu Island, but recently found also in Butrinti lagoon in Albania. The presence of this species, that almost totally replaced Adriatic dwarf goby in the Tyrrhenian lagoons under study, can be ascribed to the stockings of wild fry of commercially important species originating from Balkan countries.

Digital Artifacts and Landscapes. Experimenting with Placemaking at the Impero Project

This paper describes the public archaeology approach and placemaking experiment at the Etruscan and Roman site of Podere Cannicci in Tuscany (Italy), drawing from the previous experience at three other archaeological sites along the Tyrrhenian coast. After three years of excavations at the IMPERO Project (Interconnected Mobility of People and Economy along the River Ombrone), the team has begun a side project to develop new strategies for communicating the results of the research. These include, but are not limited to, an app which displays augmented reality and 3D reconstructions of both the site and the material culture. The project uses digital narratives to engage local communities and scholars in the interpretation and reconstruction of ancient landscapes along with the middle valley of the Ombrone river. This approach also has the potential to support and sustain local tourism, providing an original experience for visitors. Moreover, the solution allows people from all over the world to be connected with the ongoing research and its results, as everything will be published on a dedicated website.

Concentrations of trace elements in tissues of loggerhead turtles (Caretta caretta) from the Tyrrhenian and the Ionian coastlines (Calabria, Italy).

Toxic trace elements from both, natural and anthropogenic origin, pose a threat to aquatic environments and marine wildlife due to their long-range transport, bioaccumulative nature, and biomagnification through the food chain. Being long-lived and migratory animals, sea turtles can be exposed to elevated levels of toxic elements, and are therefore considered sentinel species for chemical pollution. In this study, concentrations of trace elements (arsenic, cadmium, lead, mercury) were determined in tissues of 46 loggerhead sea turtles (Caretta caretta) stranded along Tyrrhenian and Ionian coasts of Calabria, in Southern Italy, between 2014 and 2020. Curved carapace length (CCL), curved carapace width (CCW), body mass (BM), and sex were determined and the correlations of these parameters with toxic elements concentrations were investigated. During necropsy, kidney, liver, and muscle tissues were collected and the concentration and distribution of metals determined. Muscle tissues showed the lowest toxic element burdens, except for As that showed the highest mean concentrations in this tissue. The kidney was the main accumulation organ for Cd, while similar levels of Hg and Pb were measured in kidney, liver, and muscle tissues. The risk assessment performed for Cd, Hg, and Pb in sea turtles' liver highlighted possible negative effects on sea turtles' health and the need for marine turtle toxicology researches. This is the first study reporting levels and distribution of toxic elements in tissues of Caretta caretta turtles from the Tyrrhenian and Ionian coasts of Calabria.

Coastal Water Remote Sensing From Sentinel-2 Satellite Data Using Physical, Statistical, and Neural Network Retrieval Approach

Recent optical remote sensing satellite missions, such as Sentinel-2 with the MultiSpectral Imager (MSI) onboard, allow the estimation of coastal water key parameters with very high spatial resolutions (down to 10 m). In this article, multiple approaches are proposed for retrieving chlorophyll-a (Chl-a) and total suspended matter (TSM) along the Adriatic and Tyrrhenian coasts in Italy, using both empirical and model-based frameworks to design regressive and neural network (NN) estimation methods. The latter proves to be more accurate on a regional scale, where standard ocean color physical models exhibit high uncertainty in their local parameterization due to the complex spectral characteristics of the observed scene. Retrieval results are encouraging for Chl-a with a coefficient of determination R <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> up to 0.72 with a root-mean-square error (RMSE) of 0.33 mg m <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-3</sup> , using an empirical NN. The TSM algorithms exhibit higher uncertainty, mainly due to scarcity of in situ measurements and model parameterizations, with R <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> = 0.52 and RMSE = 1.95 g/m <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sup> using NNs. The bio-optical model, used for the development of model-based algorithms, shows some inadequacies in representing the inherent and apparent optical properties for the case study areas, especially considering the different spectral features between the oligotrophic Tyrrhenian Sea and the eutrophic Adriatic Sea. This study confirms the potential of Sentinel-2 MSI products for coastal water monitoring, but it also highlights key issues to be further tackled such as the atmospheric correction impact, the need of reliable in situ measurements, and possible bathymetry effects near the shores.

Spatial and Temporal Distribution of Chemically Characterized Microplastics within the Protected Area of Pelagos Sanctuary (NW Mediterranean Sea): Focus on Natural and Urban Beaches

Data on the abundance and distribution of Anthropogenic Marine Debris (AMD) on the coastal areas of the northern Tyrrhenian coast are still scarce. The objective of this study is to characterize, in terms of size, color, morphology and polymeric nature, the Large Microplastics (LMPs), i.e., plastic objects within 1 and 5 mm, sampled on three beaches located within the coastal macro-area of the Pelagos Sanctuary, an international protected zone in the north-western Mediterranean. The beaches have similar morphological characteristics but different degrees of urbanization. LMPs were sampled seasonally for one year. The polymeric nature of a representative subsample of the collected LMPs was investigated using a portable Raman instrument, to assess the feasibility of in situ characterization. In this study, 26,486 items were sorted by typology (Expanded Polystyrene-EPS, fragments, and resin pellets), size, and for fragments and resin pellets, also by color and chemical nature. Statistical data on the quantity, density, type, spatial distribution, and seasonality of the sampled LMPs are presented. Differences in LMP abundance and composition were detected among sites. A seasonality trend emerges from our statistical analysis, depending on both LMP typology and urbanization degrees of the beaches. Our data do not show the existence of a relationship between the size of the investigated MPs and their color, while they suggest that the type of polymer influences the degree of fragmentation. This underlines the need to further investigate the mechanisms leading to the production and dispersion of MPs in coastal areas, taking into account both the urbanization of the beach, and therefore the possible sources of input, and the different types of MPs. Finally, a Raman portable instrument proved to be a valuable aid in performing in situ polymeric characterization of LMPs.

Disproving the Presence of Paleozoic‐Triassic Metamorphic Rocks on the Island of Zannone (Central Italy): Implications for the Early Stages of the Tyrrhenian‐Apennines Tectonic Evolution

AbstractThe inner Apennines (Italy) are characterized by scattered outcrops of continent‐derived orogenic metamorphic units exposed along the Tyrrhenian coast from northern to southern Apennines. At least since the 1970s, some peculiar rocks exposed on Zannone Island (central Italy) have been described as the only Paleozoic‐Triassic metamorphic complex linking those exposed in the northern—with those in the southern Apennines. Assessing the protolith nature, thermobaric conditions, and structural features of what is accepted to be the metamorphic unit of Zannone is, therefore, crucial to elucidate the early paleotectonic evolution of the Apennines‐Tyrrhenian orogenic system. To that end, we interpreted seismic reflection profiles offshore Zannone, we carried out mesoscale and microscale structural investigations of representative outcrops on the island, performed X‐ray diffraction analysis, and K‐Ar and U‐Pb geochronology of representative clay gouge and syntectonic carbonate veins. Results show that the metamorphic rocks of Zannone can actually be reinterpreted as belonging to nonmetamorphic siliciclastic turbidites, likely deposited in foredeep settings, and coeval to the Oligocene‐Miocene Macigno Fm. of the northern Apennines. The turbiditic sequence was overthrust by Triassic dolostone in the early Miocene (~22 Ma), weakly deformed at &lt;200°C, and downfaulted by postorogenic extensional faults starting ~7 Ma ago. Hence, Zannone represents a unique geological location in central Italy where to study the innermost (i.e. oldest) thrust sheet of the central‐northern Apennines, thereby shedding new light onto the early tectonics of the Apennines. Based on this evidence, a new evolutionary scheme for the early stage of the Apennines tectonic evolution is proposed.

Exploring the resident gut microbiota of stranded odontocetes: high similarities between two dolphin species Tursiops truncatus and Stenella coeruleoalba

AbstractThe evaluation of symbiotic microbial communities occurring in the intestinal tract of animals has received great interest in recent years. However, little is known about gut microbial communities in cetaceans, despite their relevance in the ecology of marine communities. Here, we report an investigation using 16S rRNA gene amplicon sequencing of the resident gut microbiota of the two cetacean species Stenella coeruleoalba and Tursiops truncatus by sampling intestinal mucosa from specimens retrieved stranded along the Tyrrhenian coast of Tuscany (Italy). We found an abundance of members from Clostridiaceae and Fusobacteriaceae, which in total accounted for more than 50% of reads, in agreement with gut microbiota composition of other carnivorous mammals. Probably due to the limited number of samples available, sex, preservation status and also species, did not correlate with overall differences in the microbiota. Indeed, a high similarity of the taxonomic (family-level) composition between the gut microbiota of the two species was found. However, Pedobacter spp. was found abundant in amplicon sequencing libraries from S. coeruleoalba, while clostridia were more abundant from T. truncatus samples. Our results shed some light on the gut microbiota composition of two dolphin (S. coeruleoalba and T. truncatus) species, with specimens collected in the wild. Studies with a larger number of individuals are now needed to confirm these first results and evaluate the interspecific differences in relation to sex and age.

Hierarchical Factor Classification of Dendrochronological Time-Series

In this paper, Hierarchical Factor Classification (HFC), an exploratory method of classification of characters is introduced, in comparison with Principal Component Analysis (PCA) in order to show its advantages, in particular when dealing with time series. Exploratory data analysis may play a very relevant role in the understanding of the structure of a data set prior the use of statistical methods – as hypothesis testing and inference, and models. The study of tree-rings time series through exploratory methods may also take advantages, by allowing some interpretation to be further checked via a small number of statistical tests. In particular, while providing overall results close to those of PCA, HFC complements it, by providing a classification of the time-series and estimating a representative chronology for each group, common to the clustered ones. As case study, a data set is taken from literature, composed by five synchronous 79 years-long chronologies of Pinus pinea L., from five different populations scattered along the Tyrrhenian coast in peninsular Italy. HFC suggests how conveniently aggregate the chronologies, by showing similarities and differences between them, otherwise unnoticed, suggesting to limit the aggregation to three chronologies only.

Chemical Profile, Antioxidant, Anti-Inflammatory, and Anti-Cancer Effects of Italian Salvia rosmarinus Spenn. Methanol Leaves Extracts.

In this study, we evaluated and compared the chemical composition, the antioxidant, anti-inflammatory, and anti-proliferative effects of four methanol extracts (R1–R4), of Salvia rosmarinus Spenn. in two different sites of Southern Italy obtained by maceration or ultrasound-assisted extraction. Extracts of S. rosmarinus collected on the Ionian coast are indicated with the abbreviations R1 (maceration) and R2 (ultrasound-assisted extraction). Extracts of S. rosmarinus collected on the Tyrrhenian coast are indicated with the abbreviations R3 (maceration) and R4 (ultrasound-assisted extraction). The chemical composition was analyzed using High Pressure liquid chromatography–Diod-Array detection–Electrospray ionization–Quadrupole–Mass Spectroscopy (HPLC-DAD-ESI-Q-MS). The antioxidant activity was analyzed by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) 2,2-diphenyl-1-picrylhydrazyl (DPPH), β-carotene bleaching, and Ferric Reducing Antioxidant Power (FRAP) assays. Antioxidant features were also assessed in lipopolysaccharide (LPS)-stimulated RAW-264.7 murine macrophages, evaluating Reactive Oxygen Species (ROS) production; in the same experimental model, the anti-inflammatory activity of the extracts was investigated. Interestingly, all extracts displayed antioxidant and anti-inflammatory properties. They exhibited significative nitrite production inhibitory activity, whith IC50 values ranging from 3.46 to 5.53 µg/mL, without impairing cell viability. The anti-inflammatory activity was also investigated by Western Blotting and immunofluorescence assay, highlighting the R3 and R4 extracts ability to reduce NF-κB translocation, as well as to disrupt the MAPKs signaling pathway. Extracts exhibited both potential anti-proliferative activity on breast cancer cells, inducing apoptosis, without affecting non-tumorigenic cells, and the ability to inhibit MDA-MB-231 cells’ motility. Finally, the rosemary extracts treatment significantly reduced the power of conditioned media, from MCF-7 or MDA-MB-231 cells to induce nitrite production on RAW 264.7 cells, confirming their promising anti-inflammatory activity.

WRF wind field assessment under multiple forcings using spatialized aircraft data

AbstractThe performances of limited area weather models are affected by the choice of core solvers, domain resolutions, and initial and boundary conditions. To understand the extent of such differences on simulated wind fields, weather research and forecast (WRF) simulations initialized by different forcings were extensively compared with an aircraft‐derived high‐resolution data set. The two used forcings were the European Centre for Medium‐Range Weather Forecasts (ECMWF) ERA‐Interim reanalysis and the National Centers for Environmental Predictions (NCEP) Climate Forecast System Reanalysis (CFSR). The model domain covered a large portion of central western Italy (including part of the Tyrrhenian coast) encompassing the aircraft track and allowed the characterization of their performance across the simulation domain rather than a small set of point‐based observations. The WRF results show good agreement with the aircraft data across the whole flight track with both forcings (root mean square errors (RMSEs) &lt; 2.3 m·s−1 and an average r2 = 0.7). Orography and coasts show an effect on simulated wind fields. The presence of a strong orography (which is smoothed by the model internal terrain elevation model) is associated with increased errors. Distance from the coast is also associated with a variation in RMSE (even if in a non‐straightforward manner) because of potential breeze effects. No forcing data set clearly outperforms the other, while the ECMWF has higher correlation co‐efficients when considering wind direction.

Host biological factors and geographic locality influence predictors of parasite communities in sympatric sparid fishes off the southern Italian coast

Host biological factors and habitat influence the faunal assemblages and biodiversity worldwide, including parasite communities of vertebrate and invertebrate hosts. The ecological relationship between hosts and parasites can be mediated by interaction of host’s biological factors, as their physiological condition, diet and size, with the environmental components, somehow influencing the features of parasite infection in host populations. Here, we used boosted regression tree models to study the parasite communities of two sympatric sparid fishes, the salema Sarpa salpa and the white seabream Diplodus sargus, to investigate the role of specific host’s traits in two contiguous coastal areas along the southern-western Tyrrhenian coast of Italy characterized by different degree of deterioration. Results showed that overall and across all parasite groups (ecto-, endo- and ecto- plus endo-parasites), sampling localities were the most important predictors of abundance, species richness, and diversity for salema. Moreover, seasonality was the main predictor of endo-parasite abundance, while size-related factors explained most of the variation in species richness and diversity. In the white seabream, size-related factors and reproductive cycle-related factors were the most important predictors for the overall parasite abundance and parasite richness, respectively. Our findings suggest that the parasite community of salema and white seabream responded differently to specific biological factors, highlighting how the environmental conditions under which they live may exert a strong influence on the parasite communities of each host fish.

How the South was won: current and potential range expansion of the crested porcupine in Southern Italy

Since the 1970s, the crested porcupine Hystrix cristata has expanded its range into Northern regions in Italy, where it was historically absent, helped by climatic change, legal protection, and forest re-expansion. In the past 10 years, a remarkable range expansion has also been observed in the southernmost Italian regions. The aim of our work was to summarize the distribution of this species in Southern Italy and assess potential range expansion under multiple future scenarios of global warming. We collected 1783 occurrence records of the crested porcupine through ad-hoc web pages, online platforms, and some data directly collected by authors (N = 976 occurrences between 1998 and 2008; N = 807 between 2008 and 2019). A remarkable increase in porcupine records occurred in Lucania, Campania, and Apulia regions, in Southern Italy, mostly along the coastlines. Species distribution models showed that porcupine presence is associated with warm temperatures and an intermediate level of precipitation. Although land-cover showed lower importance compared to climate, the species was positively associated with forests and negatively associated with agricultural, grasslands and shrublands, and urban landscapes. Model projections suggested that future global warming can improve suitability for porcupines in the Apennine ridge, including the Southern Calabria and the Aspromonte National Park. However, increase in drought and urbanization may reduce the habitat suitability for the crested porcupine in the Salento peninsula, limiting the success of the range expansion by this large rodent, and in Eastern Calabria, wih possible range contractions along the Tyrrhenian coast.

The Essential Oil of Salvia rosmarinus Spenn. from Italy as a Source of Health-Promoting Compounds: Chemical Profile and Antioxidant and Cholinesterase Inhibitory Activity.

The chemical composition of the essential oil from Salvia rosmarinus Spenn. collected in Calabrian Ionian (R1) and Tyrrhenian (R2) coast (Southern Italy) was examined by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). Essential oils are mainly characterized by monoterpene hydrocarbons (39.32–40.70%) and oxygenated monoterpenes (36.08-39.47%). The 1,8-cineole, α-pinene, camphor, and trans-caryophyllene are the most representative compounds. S. rosmarinus essential oils were investigated for their antioxidant activity by using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), ferric reducing ability power (FRAP), and β-carotene bleaching tests. Additionally, acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory activity assays were used to screen the neuroprotective effects of S. rosmarinus. R2 showed the highest antioxidant potential as confirmed by relative antioxidant capacity index (RACI) and exhibited a selective activity against AChE (half maximal inhibitory concentration, IC50, value of 41.86 μg/mL). These results suggest S. rosmarinus essential oil as a potential source of bioactive compounds.

Mercury and cadmium in striped dolphins (Stenella coeruleoalba) stranded along the Southern Tyrrhenian and Western Ionian coasts

Pollution from heavy metals is becoming a serious and widespread problem due to their persistent and bioaccumulative nature, moreover in the Mediterranean Sea, threatening food safety and the health of humans and marine animals. Cadmium and mercury in particular, are considered two of the most toxic metals to living organisms. Their presence is associated with the contribution of human activity, implying an increased level in the different environmental compartments and the inevitable bioaccumulation in the food chain.In this study, levels of cadmium and mercury were determined in liver, kidney, and muscle tissue of dolphinid specimens of Stenella coeruleoalba stranded in different locations along the coastal areas of the Tyrrhenian and Ionian Sea in Southern Italy, during the period 2015–2018 by Atomic Absorption Spectrophotometry. Data were compared with those reported for other locations along the Mediterranean sea. The correlations between biometric data (body length, weight and gender) and cadmium and mercury concentrations in samples of cetaceans were statistically analysed in order to investigate the risk these contaminants may pose to the delphinids health.Examination of the pattern of contaminants revealed a significantly high distribution for mercury in all the matrices analyzed (liver, kidney and muscle tissue). On the contrary, elevated concentrations of cadmium were found only in liver (range: 0.005 - 8.95 mg/kg w.w.) and kidney (range: 0.005 - 34.1 mg/kg w.w.) due to accumulator role of these organs in long-term exposures.

Holocene Evolution of the Burano Paleo-Lagoon (Southern Tuscany, Italy)

The study of Burano paleo-lagoon—Wetland of International Value, has allowed us to better define and extend the reconstruction of the Holocene paleoenvironmental evolution of the paleo-lagoons previously studied, located on the Tyrrhenian coast in central Italy. The investigated area is located in Southern Tuscany near the Burano Lake. The area was investigated by means of field surveys, historical maps, 16 coring, sedimentological, palynological and microfaunal analyses (foraminifera and ostracods), combined with robust geochronological control provided by 52 datings (14C and OSL). The study allowed us to reconstruct the environmental and morphological evolution of the Burano paleo-lagoon during the last 8000 years and to hypothesize a Rise Sea Level (RSL) curve. In this context, 5 main evolutionary phases have been recognized. (1) before 7.5 ka BP in the southern-eastern part, an open lagoon developed; (2) ~6 ka BP a barrier-lagoon system develops throughout the entire area and the lagoon progressively changed from open to closed one; (3) ~5 ka BP the width of the lagoon increases and a lacustrine facies appears along the entire axis of the coastal basin; (4) ~4 ka BP the lacustrine facies shows a discontinuous distribution respect to the previous phase; (5) during the last 4 ka the lacustrine facies disappear and the lagoon turns into a wetland area.

Brittle vs. ductile strain during the synorogenic exhumation of HP-LT rocks: An example from the Lungro-Verbicaro Unit mylonites (northern Calabria, Italy)

We present a study on the mylonites exposed in the Cirella area along the Tyrrhenian coast of northern Calabria (southern Italy), in order to understand the role of ductile and brittle deformation on the exhumation process of these rocks. The analyzed shear zone is located in the footwall of a regional tectonic contact between metaophiolites (Ligurian Complex) on the top, and the continental Lungro-Verbicaro Unit (Adria plate) at the bottom. The latter consists of a metasedimentary succession with Triassic shallow-water carbonates at the base of it, evolving upward to Jurassic-lower Miocene slope to deep basin carbonates and foredeep siliciclastic rocks. Both oceanic and continental successions are characterized by high-pressure and low-temperature (HP-LT) metamorphism. The analyzed mylonites show different degrees of strain, ranging from protomylonites to ultramylonites, the latter characterized by well-marked foliation and stretching lineations. Several micro- and mesoscale progressive deformation structures, including two fold sets, S-C’ structures are present in these rocks, and a late oblique foliation, indicating a NW sense of shear. A successive shortening stage produced thrust faults and related folds, indicating a tectonic transport toward SE. We associate the mylonitic deformation with the activity of the overlying detachment fault, which is responsible for the synorogenic exhumation of the Lungro-Verbicaro Unit during the Miocene time. However, the ductile strain, recorded by the studied mylonites, accounted for a small displacement during the exhumation of this tectonic slice. In fact, the calculated displacement achieved by the Lungro-Verbicaro Unit, during exhumation, is much larger than the one estimated considering only the ductile zones activity. This evidence suggests that, synchronously with ductile strain, brittle deformation occurred along the detachment fault located at the top of the thrust sheet. In addition, a severe erosion due to the tectonic exhumation of the HP-LT rocks (in the late Miocene) produced a large amount of clastic sediment that filled the extensional basins widespread in northern Calabria.

The Mediterranean Mussel (Mytilus galloprovincialis) as Intermediate Host for the Anisakid Sulcascaris sulcata (Nematoda), a Pathogen Parasite of the Mediterranean Loggerhead Turtle (Caretta caretta).

Sulcascaris sulcata (Anisakidae), a pathogenic nematode of sea turtles, may cause ulcerous gastritis with different degrees of severity. Previous studies demonstrated a high prevalence of infection in the Mediterranean loggerhead turtle (Caretta caretta), although no data on the potential intermediate hosts of this nematode has been published thus far from the Mediterranean basin. Here, using molecular analyses, we demonstrated that the cross sections of nematode larvae observed histologically in Mediterranean mussels (Mytilus galloprovincialis) collected from a farm along the Tyrrhenian coast of southern Italy belong to S. sulcata. The BLAST analysis of sequences at the ITS2 region of rDNA and mtDNA cox2 gene loci here obtained from samples of two Mediterranean mussels containing nematode larvae showed 100% homology with those at the same gene loci from the adults of S. sulcata collected from the Mediterranean Sea and deposited in GenBank. To our knowledge, this study is the first to present data on a potential intermediate host of S. sulcata in the Mediterranean basin and to report a nematode parasite from the Mediterranean mussel.

Imperial Roman mobility and migration at Velia (1st to 2nd c. CE) in southern Italy

Mobility and human migration are seen as hallmarks of Roman society. With increasing territorial expansion throughout the Mediterranean region during the Imperial Roman period, wider opportunities for both self-driven and forced mobility became possible. This study analyzes δ18O and 87Sr/86Sr values from the dental enamel of 20 human second molars (M2) to examine for potential instances of mobility at the 1st to 2nd c. CE site of Velia, located on the Tyrrhenian coast of southern Italy. Velia served as a secondary port and was utilized for the shipment of goods, boat maintenance, fish processing and arboriculture. Bagplot analysis indicates that at least 10% (n = 2/20) of the individuals sampled immigrated to Velia from non-local regions. The remaining 18 individuals show mixed signs of local residency and local mobility. Comparison of the Velia data with the contemporaneous southern Italian Imperial Roman (1st to 4th c. CE) site of Vagnari indicates a similar level of mobility to both sites. Though mobility is clearly evident among the individuals sampled from Velia, mobility to Velia appears to have been less common than to larger cosmopolitan sites, such as Portus, in proximity to the capital at Rome.

Epidemiology of blood flukes (Digenea: Spirorchiidae) in sea turtles from Tyrrhenian and Adriatic Seas, off Italy

BackgroundThe Spirorchiidae is a family of blood flukes parasitizing turtles. Spirorchiids may cause a wide range of inflammatory reactions in the vascular system of their host being frequently implicated with stranding and death of sea turtles worldwide. Recent studies revealed the presence of two spirorchiid species in the Mediterranean basin. Our study presents comparative epidemiological data of spirorchiid infections in loggerhead turtles (Caretta caretta) stranded during an eight-year period from Adriatic and Tyrrhenian Seas, and the first report of Neospirorchis Neogen-11 in a green turtle (Chelonia mydas).MethodsWe screened a total of 319 carcasses of loggerhead turtles stranded from January 2011 to December 2018 along the Tyrrhenian coast (n = 111) and the north-western Adriatic coast (n = 208) of Italy using traditional (copromicroscopy and histopathology) and molecular assays. Three green turtles from the Tyrrhenian coast were also included in the study.ResultsA total of 56 (17.5%) loggerhead turtles and one green turtle (33.3%) were found to be infected with spirorchiid flukes. Amplification, sequencing of the ITS2 region of the ribosomal RNA gene cluster and BLAST analysis confirmed the presence of Hapalotrema mistroides and Neospirorchis Neogen-11 in 51 (16.0%) and 24 (7.5%) loggerhead turtles, respectively, and Neospirorchis Neogen-11 in an infected green turtle. Differences in prevalence of infection between the two sampling areas were found.ConclusionsThe risk of spirorchiid infection in the Tyrrhenian Sea is lower than in the Adriatic Sea and in general the risk of infection in the Mediterranean is lower than in other geographical locations. Differences in the prevalence of infection between the two sampling areas were related to the differences of regional habitats supporting different abundance of spirorchiid intermediate hosts. A systematic monitoring to evaluate the progress of the infection is recommended, as well as studies on the occurrence and distribution of spirorchiid species from other Mediterranean areas.