Type For Use Research Articles

Quantitating SARS-CoV-2 neutralizing antibodies from human dried blood spots.

In the earliest days of the COVID-19 pandemic, the collection of dried blood spots (DBS) enabled public health laboratories to undertake population-scale seroprevalence studies used to estimate rates of SARS-CoV-2 exposure. With SARS-CoV-2 seropositivity levels now estimated to exceed 94% in the United States, attention has turned to using DBS to assess neutralizing antibodies within cohorts of interest. With this goal in mind, we generated contrived DBS (cDBS) and whole blood-derived DBS from convalescent and vaccinated individuals and subjected DBS eluates to a battery of assays, including a SARS-CoV-2 multiplexed microsphere immunoassay (MIA), a receptor binding domain (RBD)-human ACE2 inhibition assay (iACE2), a cell-based pseudovirus neutralization assay, and real-time PCR-based surrogate neutralization assay (NAB-Sure). The DBS results were benchmarked against paired serum samples tested in a clinically validated SARS-CoV-2 plaque reduction neutralization titer (PRNT) assay. The results of an 8-plex MIA and NAB-Sure assays demonstrated highly significant correlations with PRNT values when evaluated with a panel of 86 paired serum-DBS samples. Both the MIA and NAB-Sure are adaptable to automated liquid handlers for high-throughput capacity. While neutralizing assays were limited to the ancestral SARS-CoV-2 WA1, this study nonetheless represents an important proof of concept demonstrating the potential utility of DBS as a biospecimen type for use in assessing immunity to SARS-CoV-2 at the community and population levels.IMPORTANCESARS-CoV-2 variants of concern continue to circulate globally and remain a serious health threat to large segments of the population. From a public health standpoint, identifying vulnerable communities based on immune status is critical in terms of vaccine booster recommendations. In this report, we investigated the utility of dried blood spots (DBS) as a biospecimen type from which to estimate SARS-CoV-2 neutralizing antibody titers. Using contrived and whole blood-derived DBS, we demonstrate that SARS-CoV-2 neutralizing antibodies are readily measurable in DBS eluates and correlate with plaque reduction neutralization titer (PRNT) values from paired serum samples. Moreover, several of the methods used to estimate SARS-CoV-2 neutralizing antibodies in DBS eluates are adaptable to high-throughput platforms.

WHO International Standards for antibodies to HPV6 HPV11 HPV31 HPV33 HPV45 HPV52 and HPV58

Previously established World Health Organization (WHO) International Standards (IS) for anti-HPV16 and HPV18 antibodies are used to harmonize results across human papillomavirus (HPV) serology assays. Here, we present an international collaborative study to establish ISs for antibodies against HPV6 (NIBSC code 19/298), HPV11 (20/174), HPV31 (20/176), HPV33 (19/290), HPV45 (20/178), HPV52 (19/296) and HPV58 (19/300). The candidate standards were prepared using sera from naturally infected individuals. Each candidate was shown to be monospecific for reactivity against its indicated HPV type except for the HPV11 candidate, which was also reactive against other types. Expression of antibody levels relative to the relevant candidate IS reduced inter-laboratory variation allowing greater comparability between laboratories. Based on these results, the WHO Expert Committee on Biological Standardization established each of the 7 candidates as the 1st IS for antiserum to its indicated HPV type for use in the standardization of HPV pseudovirion-based neutralization and antibody-binding assays.

GRAFTS COMPARISON BETWEEN QUADRICEPS TENDON AUTOGRAFT VS. ALLOGRAFT FOR ANTERIOR CRUCIATE LIGAMENT REVISIOON RECONSTRUCTION: A LITERATURE REVIEW

Introduction: Anterior cruciate ligament (ACL) reconstruction is one of the most common orthopaedic surgeries performed on active people in the world. One of the most important surgical decisions is graft type for use in the reconstruction. Recently, the quadriceps tendon has gained popularity for use as a graft source for ACL reconstruction. The graft choice is broadly between an autograft or allograft. This review aims to understand the current concepts in graft comparison between quadriceps tendon autograft vs. allograft for anterior cruciate ligament revision reconstruction. Methods: This literature review study was reported following the preferred reporting items for systematic reviews and adhered to a structured review protocol. The author searched the PubMed, ScienceDirect, and British Medical Journal databases. The authors comparing selected publications reporting patient outcomes with all types of reviews or descriptions of ACL revision reconstruction and its graft being used or other related subjects. Published in English is included. The author then formulates a synthesis to support the previously determined research objectives. Results: ACL reconstruction with partial thickness soft tissue quadriceps tendon graft is a reliable option. Where there are differences in the failure rates of ACL reconstructions between allografts and autografts, these can mostly be explained by irradiated grafts. Giving sufficient radiation to achieve sterility will likely weaken grafts and make them more likely to fail; therefore, irradiating grafts is not recommended. The evidence shows no significant differences in clinical effectiveness between autografts and non-irradiated allografts. Failure rates with both grafts are now low. Discussion: The primary goals of rehabilitation after ACL reconstruction do not vary considerably based on graft type and emphasize: (1) restoration of the full range of motion, (2) normalization of quadriceps strength, (3) improvement of overall lower extremity muscle strength, and (4) re-training of movement patterns to return to pre-injury sports participation while decreasing injury risk. Achieving full motion and quadriceps strength contributes to a rapid return to normal weight bearing, gait, and activities of daily living. In the setting of revision ACL reconstruction, there are various considerations regarding graft choice due to tunnel size or position, previous usage of other grafts, and reconstructions in the other leg, which may mean that allografts would be preferred to autografts. The other population not covered sufficiently in the literature is the elite sprinting athlete, where autograft choices may be influenced by the effect of graft harvesting on their sport. However, in most cases, allograft ACL reconstruction with non-irradiated grafts is as safe but more expensive than autograft ACL reconstruction, which is preferred as it is more cost-effective. Conclusion: There is little difference in the results of ACL reconstruction with autografts or non-irradiated allografts, with any advantage being with autografts. The cost is higher with allografts. So if autografts are available, allografts are not cost-effective.

Monitoring Cell Proliferation by Dye Dilution: Considerations for Panel Design.

High dimensional studies that include proliferation dyes face two inherent challenges in panel design. First, the more rounds of cell division to be monitored based on dye dilution, the greater the starting intensity of the labeled parent cells must be in order to distinguish highly divided daughter cells from background autofluorescence. Second, the greater their starting intensity, the more difficult it becomes to avoid spillover of proliferation dye signal into adjacent spectral channels, with resulting limitations on the use of other fluorochromes and ability to resolve dim signals of interest. In the third and fourth editions of this series, we described the similarities and differences between protein-reactive and membrane-intercalating dyes used for general cell tracking, provided detailed protocols for optimized labeling with each dye type, and summarized characteristics to be tested by the supplier and/or user when validating either dye type for use as a proliferation dye. In this fifth edition, we review: (a) Fundamental assumptions and critical controls for dye dilution proliferation assays; (b) Methods to evaluate the effect of labeling on cell growth rate and test the fidelity with which dye dilution reports cell division; and. (c) Factors that determine how many daughter generations can be accurately included in proliferation modeling. We also provide an expanded section on spectral characterization, using data collected for three protein-reactive dyes (CellTrace™ Violet, CellTrace™ CFSE, and CellTrace™ Far Red) and three membrane-intercalating dyes (PKH67, PKH26, and CellVue® Claret) on three different cytometers to illustrate typical decisions and trade-offs required during multicolor panel design. Lastly, we include methods and controls for assessing regulatory T cell potency, a functional assay that incorporates the "know your dye" and "know your cytometer" principles described herein.

Structure-borne noise of steel and concrete box girders in an urban metro system: A hybrid evaluation and parametric study

Metro systems provide an efficient, convenient, and expeditious way to travel around crowded routes. Because these systems are established in cities, residential buildings close to metro viaducts are significantly affected by structure-borne noise (SBN). Although most previous studies developed SBN prediction with a focus on the accuracy and computational efficiency of a specific bridge type, only limited studies on parameters for the SBN on bridges were carried out. Thus, eliminating SBN is still a challenging task for both the design and operation phases of bridge design. Therefore, this study integrates simplified experiments with numerical analyses to evaluate the influential parameters on the structure-borne noise level (SBNL) for two types of bridge girders, that is, the double-box pre-stressed concrete girder and the double-box steel girder. The present study also proposes an optimization method for reducing the SBNL in the bridge design and operation phases and identifies the sources of SBN for these two types of bridges. In this paper, the solutions to mitigating SBN are first reviewed and briefly introduced. Theories associated with SBN are then derived and experimentally verified using a concrete or steel plate with a unit area. A hybrid evaluation method is developed to integrate transient finite-element simulation with experimental results, and the main sources (e.g., plate thickness, train speed, fastener stiffness, and track irregularity) that induce SBN are examined by this hybrid method. Consequently, the approach to resolving the SBN problem can be optimally determined. As demonstrated in the results from the hybrid evaluation method, the track condition dominates the SBNL for both types of bridges, and the relationship between train speed and plate eigenfrequency should be carefully investigated to avoid the effect of plate resonance. The pre-stressed concrete box girder is the recommended bridge type for use in urban areas from the viewpoint of minimizing the SBNL.

Application of a modular topology optimization framework to an aerospace bracket design

Abstract In aerospace industry, optimizing designs has become inevitable in terms of weight and performance requirements. Topology optimization is the most suitable optimization type for use in the conceptual design phase. Even though academic topology optimization algorithms have a modular structure (open to development), they are often useable for a regular design domain. Alternatively, commercial topology optimization software products, on the other hand, are very useful in terms of their solution speed, accuracy, and ability to handle complex or irregular design domains. However, the user is restricted with the optimization algorithms available in the software, and these software do not usually have a modular structure. In this study, a modular topology optimization framework that combines useful features of the academic codes (e.g., modularity) and the commercial software tools (e.g., capability of easily handling complex design domains) is developed. The developed framework is tested on two popular academic topology optimization problems, followed by aerospace bracket design problem. It is observed that the proposed framework usually provides lower objective function values and converges to the optimum result in fewer iterations than the Altair Optistruct topology optimization software.

Approaches to the use of stem cells in regenerative medicine

PurposeThe goal of regenerative medicine is to replace or restore missing, dysfunctional, or damaged cells, tissues and organs of a person to reproduce their normal function. The main approaches are cell therapy, tissue engineering, and gene therapy. Postnatal neural crest-derived multipotent stem cells (NC-MSC) are a promising cell type for use in regenerative medicine. This is due to the specific features of their embryonic origin and the role of the neural crest in phylogeny and ontogeny of vertebrates. MethodsThe study used research in vitro culture (monolayer cell culture, 3D culture based on hydrogels, organotypic culture of hippocampal slices, spherogenesis, directed differentiation); flow cytometry; cytochemical, immune-cytochemical and histomorphometric analysis; molecular genetic (RNA (ribonucleic acid) isolation, PCR (polymerase chain reaction) with reverse transcription, real-time PCR, nucleic acid electrophoresis); microscopy (transmitted light, phase contrast, fluorescent, confocal laser scanning); microsurgical; statistical analysis. ResultsIn this systematic review, the results showed that recently the neural crest-derived cells have been isolated from a wide range of tissues and organs of mammals at the postnatal stage of development. These cells, at least in vitro, demonstrate the ability to self-repair and multilinear differentiation into neurons, Schwann cells, melanocytes, adipocytes, osteoblasts, chondrocytes, and other types of cells, that is, according to their functional characteristics, they are multipotent stem cells. ConclusionAccording to the obtained results, tissue sources of postnatal neural crest-derived multipotent stem cells differ considerably in the degree of invasiveness of biopsy sampling, as well as the possibility of obtaining a homogeneous population of NC-MSCs, which is important for further clinical use.

The Study of Effects of Aggregates on C.B.R. Value

Abstract: In engineering practice, the earth construction requires the compaction of the existing subgrade by improving the density and strength of the data. All types of earth structures, i.e., highways, pavements, etc., rest directly on the soil beneath them. The safety of these entities depends upon the strength/bearing capacity of the soil over which these are constructed. Therefore, a proper analysis of the soil properties and the design of their compression parameter become necessary to ensure that these structures remain stable and are safe against unequal settlements. To determine the suitability of any soil type for use as subgrade, subbase, or base material, one of the parameters generally used is the California bearing ratio (C.B.R.). The coarse aggregates available as a reinforcing material can enhance soil properties and increase its C.B.R. value. This paper will study the effects of coarse aggregates on the C.B.R. value, determine suitable size range of aggregates with their percentage, and their application for the earth structures. Keywords: Soil, CBR, Aggregate, Compaction, pavement.

Technical Note: A simple procedure for mimicking tissue samples from CITES controlled animal species for use in DNA proficiency tests

Proficiency tests are an important practice in forensic laboratories. However, the range of species available for wildlife forensic proficiency testing is currently limited, especially for laboratories in Africa and Asia where CITES-listed species are frequently tested. Here we present a proof of concept of a novel sample type for use in proficiency testing based on textured vegetable protein impregnated with the synthetic DNA of a target species. This sample can act as a substitute for animal tissue and can be shipped internationally without CITES and/or biosecurity permits. This simple procedure can help laboratories ensure compliance with international standards and guidelines.

Classification of HIV-1 virological treatment failure using the Roche cobas plasma separation card on cobas 8800 compared to dried blood spots on Abbott RealTime HIV-1

Measurement of HIV-1 viral load (VL) is essential for monitoring antiretroviral treatment (ART) efficacy. In remote settings, dried blood spots (DBS) may be used as the specimen type. However, cellular components in DBS not present in the gold standard specimen type, plasma, may result in low specificity i.e., over-estimation of VL results from DBS compared to plasma. The Abbott RealTime HIV-1 assay system has been reported to have improved specificity using DBS compared to other tests. A new specimen collection matrix, the cobas plasma separation card (PSC, Roche Molecular Systems), enables specimen collection from a finger prick or venous blood, using a multi-layer absorption and filtration design that results in a specimen similar to plasma. We performed a direct comparison between VL results from PSC tested with the cobas 6800/8800 assay (c8800) and DBS tested with the Abbott RealTime HIV-1 assay. Overall concordance between PSC and plasma around the 1000 copies/mL threshold was high (>97%). Compared to VL measured using DBS and the RealTime assay, PSC and c8800 showed improved sensitivity (96.9% vs 90.6%) and specificity (97.4% vs. 87.2%) using plasma as the reference, as there were fewer patients with VL below 1000 copies/mL in plasma in whom VL was over this threshold using PSC compared to DBS. The limit of detection for PSC was lower than for DBS (575 vs. 2314 copies/mL). cobas PSC represents a promising specimen type for use with the cobas 6600/8800 system in settings where plasma cannot be used.

Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes Ameliorate HaCaT Cell Photo-Aging.

Umbilical cord mesenchymal stem cells (UCMSCs) have been identified as a potentially ideal cell type for use in regenerative therapeutic contexts owing to their excellent paracrine secretory abilities and other desirable properties. Previous work has shown that stem cell-derived exosomes can effectively reduce skin aging, but few studies have specifically focused on the role of UCMSC-derived exosomes in this context. In this study, we isolated exosomes derived from UCMSCs grown in a three-dimensional culture system and explored their ability to modulate the photo-aging of HaCaT keratinocytes. Cell viability and proliferation were assessed using CCK8 assay, whereas wound healing and transwell assays were used to assess cell migratory capabilities. UVB irradiation (60 mJ/cm2) was used to induce photo-aging of HaCaT cells. TUNEL and SA-β-Gal staining were used to explore HaCaT cell apoptosis and senescence, respectively, whereas real-time quantitative PCR was used to assess the expression of relevant genes at the mRNA level. We found that UCMSC-derived exosomes were able to enhance normal HaCaT cell proliferation and migration while also inhibiting UVB-induced damage to these cells. These exosomes also reduced HaCaT cell apoptosis and senescence, increasing collagen type I expression and reducing matrix metalloproteinase (MMP1) expression in photo-aged HaCaT cells. Together, these findings indicate that UCMSC-derived exosomes have the potential to be used therapeutically to suppress skin aging.

Nanotubes with a structure of the «magnetic core-noble metal shell» type

Using a simple two-stage method including the electrochemical synthesis of Ni nanotubes in the pores of PET membranes and their coating with gold or platinum, nanotubes with a structure of the «magnetic core - noble metal shell» type have been synthesized. The morphology of the coating is a thin continuous film with growths of various shapes. X-ray diffraction analysis estimated separate phases of nickel (core) and noble metal (coating). The magnetic properties of coated nanotubes do not differ significantly from the properties of the initial nanotubes. The method allows one to synthesize structures such as one-dimensional nanostructures of the «magnetic core - noble metal shell» type for use in the detection of chemical and biological compounds, as magnetic carriers for the delivery of drugs and genes, which can also be used as multicyclic catalysts on a magnetic carrier.

Validity of the use of porcine bone in forensic cut mark studies.

Porcine bone is often used as a substitute for human bone in forensic trauma studies, but little has been published on its comparative mechanical behavior. The factors affecting mechanical properties and therefore selection of bone models are complex and include the age of the animal at death, and physiological loading conditions, the latter being of particular relevance when using a quadrupedal animal as a human substitute. The regional variation in hardness of adult and infant porcine bones was investigated using Vickers' indentation tests and compared to published data for human limb bones to relate differences to inherent genetic effects and loading influences, and to examine the validity of the porcine-human model. Significant differences in hardness were observed both along and around the adult porcine humerus and femur, but no significant differences were found along the length of the infant bones. Significant differences were found between the forelimb and hindlimb, but only in the infant specimens. The hardness values for porcine adult cortical bone from the femur (52.23±1.00kgmm-2 ) were comparable to those reported in the literature for adult human cortical bone from the fibula, ilium, and calcaneus. These data will help inform subject selection in terms of both species and bone type for use in future trauma studies.

Human Amniotic Epithelial Stem Cells: A Promising Seed Cell for Clinical Applications.

Perinatal stem cells have been regarded as an attractive and available cell source for medical research and clinical trials in recent years. Multiple stem cell types have been identified in the human placenta. Recent advances in knowledge on placental stem cells have revealed that human amniotic epithelial stem cells (hAESCs) have obvious advantages and can be used as a novel potential cell source for cellular therapy and clinical application. hAESCs are known to possess stem-cell-like plasticity, immune-privilege, and paracrine properties. In addition, non-tumorigenicity and a lack of ethical concerns are two major advantages compared with embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). All of the characteristics mentioned above and other additional advantages, including easy accessibility and a non-invasive application procedure, make hAESCs a potential ideal cell type for use in both research and regenerative medicine in the near future. This review article summarizes current knowledge on the characteristics, therapeutic potential, clinical advances and future challenges of hAESCs in detail.

Validation of Dried Tube Sample Format Quality Controls for the Monitoring of Viral Load and Blood Screening Assays

HIV viral load (VL) and donor screening assays experience variation and require quaity assurance (QA). NRL sought to confirm a dried tube sample format (HIVDTS) sample type for use in quality control (QC) programs for HIV molecular testing.50 μL of HIV supernatant at 1 × 105 copies per millilitre (copies/mL)) was dried for 48 hours at room temperature. Post-production and shipped integrity studies were undertaken. Dried HIVDTS was reconstituted in PBS buffer and tested in HIV VL (six participants) or blood screening assays (four participants). Results were entered into NRL’s QC monitoring software (EDCNet™) for analysis.The mean of 224 VL results when HIVDTS QCs were tested in Biocentric HIV GENERIC Charge Virale assay was 4.54 log10 copies/mL, with the percentage coefficient of variation (CV%) ranging from 1.75 to 13.20%. The mean Ct value for HIVDTS QCs tested on Roche Cobas MPX assay results was 28.71 (range 28.33 to 29.14), with CV% ranging from 1.56 to 3.98%.The study confirms HIVDTS QCs can effectively monitor the performance of HIV molecular testing and offers a cheaper alternative to commercial QC samples that require cold-chain shipping on dry ice and UN3373 conditions.

A New Electrobaric Membrane System for Separation of Solutions

A design is proposed for an electrobaric membrane system of coil type for use in the separation of solutions. The effective total working area of the membranes is calculated. The volume of solution that may be separated by the refined system here proposed is compared with that for the prototype system (described in the literature).

Comparison of glucose concentrations in serum, plasma, and blood measured by a point-of-care glucometer with serum glucose concentration measured by an automated biochemical analyzer for canine and feline blood samples.

To determine the correlation between glucose concentrations in serum, plasma, and blood measured by a point-of-care glucometer (POCG) and serum glucose concentration measured by an automated biochemical analyzer (ABA; gold standard). 152 canine and 111 feline blood samples. For each sample, the glucose concentration in serum, plasma, and blood was measured by a POCG and compared with the ABA-measured glucose concentration by means of the Lin concordance correlation coefficient. Results were summarized by species for all samples and subsets of samples with hyperglycemia (ABA-measured glucose concentration > 112 mg/dL for dogs and > 168 mg/dL for cats) and pronounced hyperglycemia (ABA-measured glucose concentration > 250 mg/dL for both species). The effect of PCV on correlations between POCG and ABA measurements was also assessed. Hyperglycemia and pronounced hyperglycemia were identified in 69 and 36 canine samples and 44 and 29 feline samples, respectively. The POCG-measured glucose concentrations in serum, plasma, and blood were strongly and positively correlated with the gold standard concentration. The PCV was positively associated with the correlation between the POCG-measured blood glucose concentration and the gold standard concentration but was not associated with the correlations between the POCG-measured glucose concentrations in serum and plasma and the gold standard concentration. Results indicated that POCG-measured glucose concentrations in serum, plasma, and blood were strongly correlated with the ABA-measured serum glucose concentration, even in hyperglycemic samples. Given the time and labor required to harvest serum or plasma from blood samples, we concluded that blood was the preferred sample type for use with this POCG.

A Research Procedure for Examining the Dynamic Properties of Multi-Wire Springs used in NR-30 Guns

The article presents a concept of the procedure for researching the dynamic properties of the multi-wire springs used in NR-30 guns. The aim of the research was to check the possibilities of determining the limit values of the flexibility of the multi-wire springs for the NR-30 gun, as well as to verify the obtained research results using those from range and static tests (after long compression). While verifying the method (selected measurements of 4 springs with varying degrees of wear), static and dynamic compression tests were performed on a spring mounted on a gun on specially prepared testing stations. The description of the data measurement and acquisition method applied should be considered an important element of the description of a testing station. Based on the obtained research results, it has been inferred that, during the striker’s backward movement, wave phenomena likely transpire in the spring. Force value fluctuations taking place during the spring’s movement are visible in the obtained diagrams. The dynamic and static test results (on springs with varying degrees of wear) allowed the authors to evaluate the impact of a multi-wire spring’s wear on the value of its force of interaction with the automation elements of an NR-30. Based on the data obtained, the assumptions for the method of evaluating multi-wire springs’ durability and using it to evaluate the suitability of a given spring type for use according to its technical state, have been elaborated. The final determination of the limit values of the springs’ parameters requires further research using a station with an ammunition belt encumbrance emulator, as well as emulation of the overloads of the kinematic arrangement of the gun while airborne.

Parameter Study on Solid Oxide Fuel Cell Heat-Up Process to Reaction Starting Temperature

Solid oxide fuel cell (SOFC) is a promising fuel cell type for use in distributed generation owing to its high energy efficiency, ability to use various fuels, generation of additional heat energy, etc. These advantages arise from its high operating temperature, but this in turn imposes limitations like difficulty in selecting proper materials, restrictions in the operating environment, difficulty of thermal management, and long on/off time. A good deal of research has been conducted on thermal analysis of SOFCs, however little or none on the heat up process parameter study; such studies are required because the heat up process influences thermal stability and efficiency in terms of both time and energy. The level of effects changes depending on the heat up conditions. Accordingly, the present work deals with two heat up conditions and their effects upon the results; these conditions are hot air mass flow rate and the hot air temperature, represented as a temperature difference relative to the SOFC stack. This study is accomplished by means of modeling using MATLAB, including validation of the model by comparison to an International Energy Agency benchmark test. The results show that both conditions affect the saturation time and temperature transition of the model, but to different degrees and in different ways. Especially, saturation time depends primarily on the temperature difference, and changes in the temperature difference between the inlet and outlet is determined by linear equation with the temperature difference as domain and the mass flow rate as slope. From the results, optimal heat up conditions proper to each SOFC geometry or material can be found by changing these variables appropriately.

Contribution to new numerical integration in dynamics

The problem of time integration in the dynamics of building structures is primarily a mathematical problem of the accuracy of the numerical integration of the acceleration time course. All existing methods are trying more or less successfully to master this problem. The best known and most widely used is the Newmark method, which by its very nature is only a trapezoidal rule applied to both acceleration and speed. The most effective method of numerical integration of repeated integrals was introduced mathematically [1], namely the modification of numerical integration methods of the Gaussian type for use on repeated integrals. In this work is introduced their simple use in problem solution of mechanics and dynamics of structures and consequently comparison of the results with the traditional Newmark Direct Time Integration method is presented.