Approaches to the use of stem cells in regenerative medicine

Abstract

PurposeThe goal of regenerative medicine is to replace or restore missing, dysfunctional, or damaged cells, tissues and organs of a person to reproduce their normal function. The main approaches are cell therapy, tissue engineering, and gene therapy. Postnatal neural crest-derived multipotent stem cells (NC-MSC) are a promising cell type for use in regenerative medicine. This is due to the specific features of their embryonic origin and the role of the neural crest in phylogeny and ontogeny of vertebrates. MethodsThe study used research in vitro culture (monolayer cell culture, 3D culture based on hydrogels, organotypic culture of hippocampal slices, spherogenesis, directed differentiation); flow cytometry; cytochemical, immune-cytochemical and histomorphometric analysis; molecular genetic (RNA (ribonucleic acid) isolation, PCR (polymerase chain reaction) with reverse transcription, real-time PCR, nucleic acid electrophoresis); microscopy (transmitted light, phase contrast, fluorescent, confocal laser scanning); microsurgical; statistical analysis. ResultsIn this systematic review, the results showed that recently the neural crest-derived cells have been isolated from a wide range of tissues and organs of mammals at the postnatal stage of development. These cells, at least in vitro, demonstrate the ability to self-repair and multilinear differentiation into neurons, Schwann cells, melanocytes, adipocytes, osteoblasts, chondrocytes, and other types of cells, that is, according to their functional characteristics, they are multipotent stem cells. ConclusionAccording to the obtained results, tissue sources of postnatal neural crest-derived multipotent stem cells differ considerably in the degree of invasiveness of biopsy sampling, as well as the possibility of obtaining a homogeneous population of NC-MSCs, which is important for further clinical use.