Turmeric Oil Research Articles

Utilization of UPLC-PDA and GC–MS/MS coupled with metabolomics analysis to identify bioactive metabolites in medicinal turmeric at different ages for the quality assurance

BackgroundAvailable monographs often suggest measurement of curcumin or curcuminoids for quality control of turmeric-based medicines/products. However, one compound is not enough to indicate the quality of traditional herbal medicines due to the holistic approach. Furthermore, to ensure high quality of such products, good harvesting practice plays an important role in the quality control of turmeric raw materials. PurposeThis study aimed to indicate quality markers of turmeric and to suggest optimum harvesting times for turmeric used for medicinal purposes by integration of Thai traditional medicine knowledge. MethodsTurmeric rhizomes at 4, 6, 8, 9, 10, and 11 months old were analyzed. UPLC-PDA was used for quantitation of curcumin. GC–MS/MS was used to obtain chemical profiles of turmeric volatile oil. PCA, Volcano plot, and HCA were performed to identify similarities or differences of the data. ResultsTurmeric aged between 6 and 10 months old contained > 5%w/w of curcumin, which complied with Thai Herbal Pharmacopoeia 2021. GC–MS/MS analysis suggested suitable chemical markers, namely Ar-turmerone, turmerone, curlone, and zingiberene, because they exhibited pharmacological activities related to the traditional uses of turmeric. Eucalyptol, santalene, β-caryophyllene, cis-β-farnesene, α-caryophyllene, curcumene, β-bisabolene, β-sesquiphellandrene, and cis-sesquisabinene hydrate were also tentatively identified. Later, the multivariate analysis revealed that turmeric aged between 6 and 10 months old showed similar metabolite profiles. ConclusionBased on curcumin content and chemical profiles, turmeric aged between 6 and 10 months old are appropriate for uses in Thai traditional medicine. Our study suggests additional information in the quality control of turmeric to assure its efficacy, especially for uses in traditional medicines.

Development of Turmeric Oil-Loaded Chitosan/Alginate Nanocapsules for Cytotoxicity Enhancement against Breast Cancer.

Turmeric oil (TO) exhibits various biological activities with limited therapeutic applications due to its instability, volatility, and poor water solubility. Here, we encapsulated TO in chitosan/alginate nanocapsules (CS/Alg-NCs) using o/w emulsification to enhance its physicochemical characteristics, using poloxamer 407 as a non-ionic surfactant. TO-loaded CS/Alg-NCs (TO-CS/Alg-NCs) were prepared with satisfactory features, encapsulation efficiency, release characteristics, and cytotoxicity against breast cancer cells. The average size of the fabricated TO-CS/Alg-NCs was around 200 nm; their distribution was homogenous, and their shapes were spherical, with smooth surfaces. The TO-CS/Alg-NCs showed a high encapsulation efficiency, of 70%, with a sustained release of TO at approximately 50% after 12 h at pH 7.4 and 5.5. The TO-CS/Alg-NCs demonstrated enhanced cytotoxicity against two breast cancer cells, MDA-MB-231 and MCF-7, compared to the unencapsulated TO, suggesting that CS/Alg-NCs are potential nanocarriers for TO and can serve as prospective candidates for in vivo anticancer activity evaluation.

Evaluating some Essential Oils-Based and Coconut Oil Nanoemulgels for the Management of Rheumatoid Arthritis

Essential oil-based and coconut oil nanoemulgels were formulated, and their anti-arthritic effect was evaluated. Nanoemulsions of lemon, ginger, turmeric, and coconut oil were formed by spontaneous emulsification. Nanoemulgels were obtained using 1 % of carboxymethylcellulose and carbopol. Characterizations included pH, transmittance, viscosity, conductivity, spreadability, and droplet size. Stability studies were performed. Arthritis was induced in Wister rats by injecting 0.1 ml formaldehyde (2 % v/v) into the sub plantar region. Paw volumes and thickness were measured for 28 days. Particles were nanosized with polydispersity indices ranging from 0.21 to 0.75. Formulations had pH of 4.96 to 6.81. Transmittances ranged from 0.02 to 95.6 %. Viscosities ranged from 308.3 to 1807 mPaS. Nanoemulgels were more stable at 25 ± 2 ºC. Anti-arthritic evaluations showed that ginger and lemon nanoemulgels exhibited higher and similar percentage inhibition patterns from days 7 to 28. Only ginger nanoemulgel reached basal paw values on day 28. Analysis of variance showed statistically significant differences (p< 0.05) in results obtained on day 7 between Voltaren® and the lemon, turmeric, and coconut groups, while on day 21, there were also significant differences (p<0.05) between the Voltaren® group and the ginger and coconut groups. Ginger nanoemulgel was most effective in treating rheumatoid arthritis.

Synergistic Effect of Some Essential Oil Extracted from Three Nigeria Spices against Some Pathogenic Organisms

Introduction: Essential oils of plants and their other products from secondary metabolism have had a great usage in folk medicine, food flavoring, fragrance, and pharmaceutical industries.
 Aims: This study sought to evaluate the phytochemical properties of the oil extracted from the medicinal plants such as; clove, turmeric and black pepper, as well as to determine the antimicrobial effect of essential oil of clove against selected pathogens.
 Methodology: Qualitative phytochemical screening of the oil extracted from the plant samples (clove, turmeric and black pepper) was determined using standard method, oil was extracted from the medicinal plant samples, and antimicrobial susceptibility test was performed with the oil extracts in various concentrations against the test organisms.
 Results: The qualitative phytochemical screening revealed the presence of alkaloids, phenols, and saponins in clove, turmeric and black pepper oil samples; however, tannins, glycosides, and flavonoids are present in clove oil, and saponins, flavonoids, and terpenoids were abundantly present in turmeric oil sample. The synergistic antimicrobial susceptibility assay of clove with turmeric and black pepper oil is more pronounced against the test bacterial with the highest zone of inhibition of 46.00 mm against Escherichia coli. The combination of essential oil of clove and black pepper oil inhibited Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Candida albicans and Streptococcus spp., except Pseudomonas aeruginosa which shows antagonistic effect.
 Conclusion: Spices such as clove, turmeric and black pepper have exhibited significant antimicrobial activities against intestinal bacteria like S. aureus, Klebsiella spp., E. coli, C. albicans, P. aeruginosa and Streptococcus spp. Therefore, these spices could be used to decrease the possibility of food poisoning, to increase the food safety and shelf-life of products, and to treat some infectious diseases.

Antifungal Efficacy of Different Extracts of Neem and Turmeric on Candida Species – An in vitro Analysis

Background: Curcumin is an age-old spice, which is known for its anti-inflammatory activity and antibacterial property, and neem is proven to have many medicinal properties, especially known for its antimicrobial properties. Aim: The study aimed at assessing the antifungal activity of different preparations of turmeric and neem over Candida species. Materials and Methods: Using Sabouraud's agar as the medium, Candida species colonies were transferred to the agar plates after preparation of five wells. 75 μl, 50 μl, 25 μl, 10 μl, and 5 μl of neem leaf oil, neem oil, turmeric oil, turmeric extract, and neem seed oil were added and incubated at 37°C for 18–24 h. The diameter of inhibition zone around the wells where the growth was inhibited corresponded to the antifungal activity. Results: Neem bark extract and turmeric extract showed the highest inhibitory range against Candida albicans. Neem leaf extract showed the highest range of inhibition at 75% against Candida krusei and Candida tropicalis. Conclusion: Extracts of turmeric and neem have shown definitive inhibition against different strains of candida in vitro, and hence, these can be used for treating candidiasis as an alternative to regular triazoles.

Effect of turmeric (Curcuma longa L.) essential oil on the oxidative stability of soybean oil

Effect of turmeric (Curcuma longa L.) essential oil on the oxidative stability of soybean oil

Network pharmacology-based dissection of the underlying mechanisms of dyspnoea induced by zedoary turmeric oil.

Zedoary turmeric oil (ZTO) has been widely used in clinic. However, the unpleasant induced dyspnoea inevitably impedes its clinical application. Thus, it is urgent to elucidate the mechanism underlying the ZTO‐induced dyspnoea. In this study, network pharmacology was firstly performed to search the clue of ZTO‐induced dyspnoea. The key target genes of ZTO‐induced dyspnoea were analysed using GO enrichment analysis and KEGG pathway analysis. GO analysis suggested that haem binding could be a key molecular function involved in ZTO‐induced dyspnoea. Hence, the haemoglobin (Hb) was focused for its oxygen‐carrying capacity with haem as its critical component binding to the oxygen. Ultraviolet–visible absorption spectrum indicated that the ZTO injection (ZTOI) perturbed the Soret band of Hb, suggesting an interaction between ZTO and Hb. GC–MS analysis revealed that β‐elemene, germacrone, curdione and furanodiene were main components of ZTOI. Molecular docking was used to illustrate the high affinity between representative sesquiterpenes and Hb, which was finally confirmed by surface plasmon resonance, suggesting their potential roles in dyspnoea by ZTO. Following a network pharmacology‐driven strategy, our study revealed an intervened Hb‐based mechanism underlying the ZTO‐induced dyspnoea, providing a reference for elucidating mechanism underlying adverse drug reactions of herbal medicines in clinic.

Review of Static and Dynamic Wireless Electric Vehicle Charging System

Abstract: Electrified transportation will contribute to the reduction of greenhouse gas emissions and the stabilization of gasoline costs. To promote the adoption of electrified transportation, a diverse network of charging stations must be established in a userfriendly environment. Wireless electric vehicle charging systems (WEVCS) have the potential to be a viable alternative technology for charging electric cars (EVs) that do not need a plug. This article discusses the present state of wireless power transfer technologies for electric vehicles. Additionally, it contains wireless transformer constructions that have been studied using a range of ferrite forms. WEVCS are linked with health and safety concerns, which have been addressed in recent international standard development. Two main applications, static and dynamic WEVCS, are discussed, and current development is documented using features from research labs, universities, and industry. Additionally, future concepts-based WEVCS are evaluated and studied, including "vehicle-to-grid (V2G)" and "in-wheel" wireless charging systems (WCS), with qualitative comparisons to other current technologies Keywords: Combustion, Diesel Engine, Turmeric Oil, Vibration Analysis, Methyl Ester, and Blends

Composition and Health Benefits of Turmeric (Curcuma longa)

Abstract: Turmeric (curcuma longa) belonging to Zingiberaceae family, it is most useful medicinal herb. Turmeric can be found in India other Southeast Asian countries. Turmeric processing steps are washing, boiling, drying, polishing, coloring, grinding/powdering and packing. Turmeric contains more than 100 chemical compounds that contribute to its healing abilities. Curcumin has strong anti-inflammatory and antioxidant properties. Other curcuminoids include bisdemethoxycurcumin and dimethoxycurcumin. Turmeric contains 8.92% moisture, 2.85 % ash, 4.60% crude fibre, 6.85% fat, 9.40% crude protein & 67.38% carbohydrates. Turmeric rhizome contains 0.89% thiamine, 0.16% riboflavin & 2.30% niacin. Turmeric is a spice used for cooking but has gained immense popularity as one of the most powerful herb for fighting various diseases. Turmeric has been shown to be one of the best foods to maintain, protect, and boost immunity, reducing the risk of falling ill. Turmeric has anti-cancer, anti-inflammatory, anti- microbial, anti-viral, and anti-bacterial properties and also fights against COVID-19 virus. Taking high dosage of turmeric does have side effects include headache, stomach upset, nausea, heart burn etc. In some studies received 0.45 to 3.6g/day curcumin for one to four months reported nausea, diarrhea and increase in serum alkaline phosphatase and lactate dehydrogenase contents.In food industry turmeric used for food preservation by increasing shelf life of various food products. Turmeric oil also used in cosmetic sector, perfumes and soap industries. Turmeric is also regarded as a ‘rasayana’ herb which is a branch of Ayurvedice medicine. Turmeric is used in Ayurveda to balance vata, pitta, and kapha, though in excess, it can aggravate pitta and vata. Turmeric products like turmeric product, essential oil are used for its color and taste in foods, it can be added to beverages and essential oil used as natural food colourant and also used to prevent skin diseases. Keywords: Turmeric, Curcuma longa, ayurvedic medicine.

Formulation and Evaluation of a Laterite Soil Based Scrubbing Effect Herbal Cleansing Bar

Cosmetics play a dynamic role for every person to have a glad and confident life. In the current scenario, herbal cosmeceuticals have more demand because they have no side effects. People often suffer from acne, whiteheads, and blackheads, so scrubbing becomes essential. The main objective of the present study was to prepare a herbal-mineral facial scrubbing effect cleansing bar. Majorly facial skin comes in direct contact with dirt, pollution, dust particles, and many dead cells. Some facial preparations are required to remove the dead cells and make the skin in fine fettle, cleaned, and nourished. The present study formulated a laterite soil-based herbal scrubbing effect cleansing bar for all typed skin using laterite soil powder. In contrast, glycerin soap base, virgin coconut oil, olive oil, bee honey, aloe vera, lime juice, turmeric, and lavender essential oil are used and evaluated by using various parameters such as physical appearance, color, odor, pH, washability, irritants, stability studies, microbial tests, heavy metals, and total fatty matter. It was given fruitful results with all the tests. The herbal formulation had characteristic pleasant order, reddish-brown in color having, light, non-irritant to the skin and quite an elegant, microbial and heavy metal safe product. Thus, the formulated scrubbing effect facial cleansing bar can be utilized efficaciously without a side effect that exfoliates makes healthy, clean, and glowing skin.
 Keywords: Facial scrub, Cosmetic, Herbal, Mineral, Laterite soil

Fabrication of chitosan-based food packaging film impregnated with turmeric essential oil (TEO)-loaded magnetic-silica nanocomposites for surimi preservation

Successful modification of chitosan (CS) film using magnetic-silica nanocomposite to encapsulate turmeric essential oil (TEO) obtained by super critical CO2 extraction for enhanced preservation of surimi was performed. TEO exhibited antioxidant and antibacterial activities against Bacillus cereus. The core magnetic nanoparticles (MNPs) were capped with porous silica (Si) to form core-shell nanocomposites, into which TEO was loaded with 75.24% encapsulation efficiency. The fabricated nanocomposite was characterized, blended with CS to cast a bionanocomposite active film and characterized for efficient impregnation of bionanocomposite. The physical and mechanical properties of film were significantly improved after adding MNPs/Si/TEO nanocomposite. Uncontrolled release of TEO from CS film resulted in bacterial growth after 6 days of storage whereas bionanocomposites exhibited a sustained release of TEO that controlled the microbial load from 4.0 log CFU/g to 2.78 log CFU/g over 14 days. The overall study demonstrated that the CS/MNPs/Si/TEO bionanocomposite film was efficient as a packaging material for prolonged shelf-life of surimi.

Pengaruh Ukuran Partikel dan Rasio Bahan Terhadap Pelarut Air pada Distilasi Minyak Atsiri Temulawak

<p>Javanese turmeric (Curcuma xanthorrhiza) essential oil is one of the curcuminoids sources that are widely used in the food and health industries. Temulawak oil extraction usually uses ethanol as a solvent which is relatively less safe for health. This study aimed to evaluate the effect of particle size and the ratio of materials to water solvents in the distillation process of Javanese turmeric essential oil. The experiment was conducted at the Chemical Engineering Research Laboratory, Muhammadiyah University, Surakarta. The treatments tested were the powder size (20, 40, 60, dan 140 mesh), the ratio of material to water solvent (w/v) consisted of 100:800, 150:750, 200:700, and a distillation time of 3 hours at a constant temperature of 100oC with an interval of distillation duration of 0.5; 1; 1.5; 2; 2.5; and 3 hours. The parameters observed were the essential oil quality: organoleptic, refractive index, pH, mass or volume of the oil produced, and curcuminoid compounds using the GC-MS method. The maximum yield of Javanese turmeric essential oil (18.01g) was obtained at a particle size of 140 mesh with a distillation duration of 1.5–2 hours, and the ratio of raw material to solvent 100:800 (g.ml-1). Based on the GCMS analysis, the highest components in the Javanese turmeric essential oil were α-curcumin (42.17%) and α-cedrene (11.14%). The results indicated that the distillation process of Javanese turmeric oil using water as a solvent can be used as an alternative to substitute ethanol as a solvent.</p>

Design of Turmeric Rhizome Extract Nano-Formula for Delivery to Cancer Cells.

Novel turmeric rhizome extract nanoparticles (TE-NPs) were developed from fractions of dried turmeric (Curcuma longa Linn.) rhizome. Phytochemical studies, by using HPLC and TLC, of the fractions obtained from ethanol extraction and solvent–solvent extraction showed that turmeric rhizome ethanol extract (EV) and chloroform fraction (CF) were composed mainly of three curcuminoids and turmeric oil. Hexane fraction (HE) was composed mainly of turmeric oil while ethyl acetate fraction (EA) was composed mainly of three curcuminoids. The optimal TE-NPs formulation with particle size of 159.6 ± 1.7 nm and curcumin content of 357.48 ± 8.39 µM was successfully developed from 47-run D-optimal mixture–process variables experimental design. Three regression models of z-average, d50, and d90 could be developed with a reasonable accuracy of prediction (predicted r2 values were in the range of 0.9120–0.9992). An in vitro cytotoxicity study using MTT assay demonstrated that the optimal TE-NPs remarkably exhibited the higher cytotoxic effect on human hepatoma cells, HepG2, when compared with free curcumin. This study is the first to report nanoparticles prepared from turmeric rhizome extract and their cytotoxic activity to hepatic cancer cells compared with pure curcumin. These nanoparticles might serve as a potential delivery system for cancer therapy.

Introduction and Extraction Methods for Polyherbal Face Wash Formulation

A cleanser is a product that is used to get rid of make-up, lifeless skin cells, oil, dust, and different types of bacteria, microbes from the pores and skin of the face. This facilitates to unclog pores and prevent skin situations which includes zits. In the present work an try became made to formulate & evaluate face wash. Due to the fast improvement of urban towns inside the current many years the extent of pollution within the air has elevated exponentially, disturbing the development of new versions of private care merchandise. Face being most exposed a part of the body, comes in touch with pollutants very without difficulty making it hard to hold a healthy, brilliant and clear skin. The facial pores and skin is thinner and extra fragile in comparison to the relaxation of the frame. A cleansing product in particular formulated for face have to be used which will maintain the moisture and pH stability of skin. Activated charcoal has homes of deep cleansing and adsorbing impurities. This makes it a beneficial component for pores and skin care merchandise. Acne is one of the maximum typical pores and skin disorder affecting nearly all teens, as it's miles persistent with frequency remission and exacerbations is regularly associated with terrible psychological consequences, finished shallowness and social withdrawal. The Modern measures concerning the treatment of acne are extra or much less failure. Further it has some barriers, cost effective and plenty of side results. To triumph over from a lot of these, a very good natural technique ought to be reached. So we decided to use Luffa Cylindrica oil, Manjistha, Carrot, guava seed extract, Turmeric oil.

The Influences of Black Pepper, Turmeric and Fennel Essential Oils Supplementation in Feed on Egg Quality Characteristics of Layers

The Influences of Black Pepper, Turmeric and Fennel Essential Oils Supplementation in Feed on Egg Quality Characteristics of Layers

Comparative effectiveness of turmeric (Curcuma longa), garlic (Allium sativum) and curry leaves (Ocimum africanum) against stored insect pest of Maize, (Sitophilus zeamais Motsch)

The bioactivity of turmeric, garlic and curry oil and powder were evaluated and compared against maize storage pest, Sitophilus zeamais (Motsch.) at 28+20C and relative humidity (r.h.) 65+5% in the laboratory. Ten pairs of newly emerged weevils were introduced separately into each of the plastics containing 20 g maize seed treated with plant oil at different concentrations, 0.25%, 0.50% and 1.00% v/v and similarly plant powder at dosage levels of 0.25 g, 0.50 g and 1.00 g of the tested plant parts. Each treatment, including the control comprised four replicates which were laid out 3×3 factorial arrangements in a completely randomized design. Results indicated that oil and powder of curry, and turmeric evoked significant mortality (82.50%) and (81.00%), while garlic powder is equally effective (72.00%) in achieving mortality of the weevil. There was an appreciable reduction in means of exit hole and percent seed damage in treated maize seeds at 0.50% and 1.00% concentrations. Curry had exit hole (12.25 &11.00), turmeric had (12.75&11.75); also lower seed damage was recorded in curry (11.00% & 21.75%), while turmeric had (20.75% &31.00%). There was however corresponding high percent undamaged seed recorded in curry (89.00% & 78.25%) and turmeric (79.25% & 73.25%). All the plant parts tested reduced weight loss in the stored maize seeds when compared with the control. Adult mortality significantly correlated with exit hole (r = 0.381), seed damage (r = 0.0509) and undamaged seed (r = -0.0643). Therefore, 0.50% was lowest concentration at which control was achieved.

Retraction: Isolation of curcumol from zedoary turmeric oil and its inhibitory effect on growth of human hepatocellular carcinoma xenografts in nude mice

Retraction: Isolation of curcumol from zedoary turmeric oil and its inhibitory effect on growth of human hepatocellular carcinoma xenografts in nude mice

RETRACTED ARTICLE]Isolation of curcumol from zedoary turmeric oil and its inhibitory effect on growth of human hepatocellular carcinoma xenografts in nude mice

This study investigated the inhibitory effect and mechanism of curcumol on growth of human hepatocellular carcinoma (HCC) HepG2 xenografts in nude mice. Curcumol was isolated from from zedoary turmeric oil. The HepG2 xenograft model in nude mice was established. Forty modeled nude mice were divided into model, cisplatin, curcumol and cisplatin+curcumol groups. The latter three groups were treated with 2 mg/kg cisplatin, 100 mg/kg curcumol and 2 mg/kg cisplatin combined with 100 mg/kg curcumol, respectively, once every two days, for total seven times. After treatment, compared with cisplatin group, in cisplatin+curcumol group the tumor weight and tumor inhibition rate were obviously increased, the apoptosis rate of HepG2 cells was obviously increased, the expression levels of Caspase-3 and B-cell lymphoma-2 associated X proteins in xenograft tumor were significantly increased, and the expression level of B-cell lymphoma-2 protein was significantly decreased. In addition, the thymus index and spleen index of animal had no significant difference between cisplatin group and cisplatin+curcumol group. In conclusion, the additional use of curcumol can obviously increase the inhibitory effect of cisplatin on growth of HCC HepG2 xenografts in nude mice. The mechanism may be related to the enhanced promotion of apoptosis of tumor.

Turmeric Oil: Composition, Extraction, Potential Health Benefits and Other Useful Applications

The turmeric essential oil of Curcuma species has extensively more useful properties due to its rich phytochemical profile. The concentration of volatile chemical constituents varies according to their type of applied plant part (i.e., root, rhizome, leaves, and flower) for extraction and type of the adopted extraction method. Novel extraction and purification methods, subcritical CO2 , supercritical CO2 , pressurized liquid extraction, and molecular distillation are found to be more efficient for good recovery of this volatile oil, along with increased concentrations of specified compounds. Not only have the curcuminoid compounds had a broad potential in the field of pharmacology but also the turmeric oil is found to have great applicability in treating several diseases and disorders. Turmeric oil possesses good antioxidant, antimicrobial, anticancer, anti-hyperlipidemic anti-inflammatory, anti-diabetic, and hepato-protective properties. Apart from medicinal fields, this oil has also a great future in the cosmetics, pesticide, and food industries due to its rich chemical profile. The present review focuses on providing information about turmeric oil in terms of its physicochemical properties, chemical composition, and available traditional extraction techniques, as well as available novel extraction options, actual health benefits, and other useful applications. It is hoped that the reported information is helpful for further discovery in the area of food, pharmaceutical, and cosmeceutical applications.

The Healing Analysis of II Degree Burn from Surgery Essential Oil Ointment (Curcuma Longa) in Wistar Rats (Rattus Norvegicus)

Data in Indonesia the death rate from burns is still high at around 40%, mainly caused by severe burns. The most potential medicinal turmeric plant is its rhizome, which contains the phenolic compound curcumin and essential oils. Some of the benefits of turmeric antioxidants, anti-inflammatory, anti-cancer, acetylcholinesterase activity inhibitors, anti-fungal, and anti-bacterial. This study aims to find out the phytochemical content and effects of Turmeric Essential Oil Ointment (Curcuma longa Linn) in curing grade II burns in Wistar rats (Rattus norvegicus). This type of research is experimental with a pre-post approach and post-test control group design. The study was conducted at Riwandi Pet Shop and Animal House March-May 2021. The research sample is turmeric (Curcuma longa) obtained from a traditional market in the city of Medan and identified in herbarium Medanese (MEDA), Laboratoirum Taxonomy of Plants, Department of Biology, Faculty of Mathematics and Natural Sciences (FMIPA), University of North Sumatra, Medan. The test animal is a Wistar strain rat with as many as 20 tails divided into 4 treatment groups, so each group consists of 5 rats. The results of the study contained significant differences in the epithelial period of the standard group, 10% turmeric ointment and 15% against the control group. However, in the turmeric ointment group and the standard group, there was no difference in epithelialization periods. This is evident from the value P < 0.05 (Value P = 0.029). The healing effects of burns possessed by Turmeric ointment are both 10% and 15% and nebacetin ointment as standard shows significant differences. Where the wound contraction rate of turmeric ointment is 15% better than nebacetin ointment as standard. But in both turmeric ointments, both 10% and 15% and nebacetin ointment as standard did not show significant differences in the parameters of the epithelial period.