Three new steroidal saponins (1–3), together with four known compounds (4–7), were isolated from the roots and rhizomes of Rohdea chinensis Baker, and their structures were determined as (24S, 25 R)-1β-hydroxy-3β-[(β-D-glucopyranoside)oxy]-spirost-5-en-24-yl-β-D-glucopyranoside (1) and (24S)-spirost-25(27)-en-1β, 3β, 4β, 5β, 6β, 24β-hexahydroxy-24-O-β-D-glucopyranoside (2), (22S, 25S)-1β, 3β, 4β, 5β, 26, 27-hexanol-furospirost-5, 26-O-β-D-glucopyranoside (3), together with four known compounds 3-epi-diosgenin-3-β-D-glucopyranosid (4), 3-epiruscogenin (5), 25(R)-1β-hydroxy-spirost-5-en-3α-yl-O-β-D-glucopyranoside (6) and tupichinin A (7), on the basis of physico-chemical properties and spectral analysis. In this study, compounds 1–3 and 5–7 were evaluated for their cytotoxic activity against SW620, A549 and HepG2 tumor cell lines. Among them, compound 7 showed moderate cytotoxicity against two human cancer cell lines A549 and HepG 2 with IC50 values of 25.3 ± 2.6 and 26.1 ± 2.5 μM, respectively.