Tukey's Honest Significant Difference Research Articles

NON-CIRCULATION HYDROPONIC LETTUCE (Lactuca sativa L. var. Rincon) PRODUCTION USING COMMERCIALLY AVAILABLE NUTRIENT SOLUTION

This study assessed the production performance of lettuce using different simple nutrient addition programs (Yamasaki, MasterBlend, SNAP, Nutrihydro and Hydroplus) compared to organic fertilizer (Biovoltin) with water as a negative control in a Kratky hydroponic system. The crop experiment was conducted at the Institute of Agriculture, Camiguin Polytechnic State College – Catarman Campus, Tangaro, Catarman, Camiguin from January 25, 2022 to March 10, 2022. The study was laid in a Randomized Complete Block Design with seven (7) treatments and three (3) replications at eight plants per treatment. Tukey's Honest Significant Difference (HSD) was used to compare the significant differences between treatment means. Results showed a highly significant differences in the horticultural and root development characteristics, yield parameters, nutrient solution consumption and quality of nutrient solution, and sensory quality except for the survival rate, non-marketable head weight, and pH at 31 DAT. Our results showed that the use of SNAP solution could be used effectively to increase the overall production performance of lettuce. The use of Nutrihydro, Hydroplus, Yamasaki and Masterblend were also promising for its production performance. It can be concluded that the nutrient solution affects the production performance of lettuce in a hydroponic production system. However, the potential use of these various simple nutrient addition programs should be further tested for verification at different growing seasons to elicit substantial conclusions.

Evaluation of Surface Roughness in Clear Silicon Fabricated Using Three Different Techniques: An In-vitro Study

Introduction: Anterior composite restorations present many aesthetic challenges for clinicians. Direct veneers provide chairside advantages such as evaluating tooth anatomy, shade selection, and correcting tooth morphology according to the patient’s desire. In today’s world, the use of digitalisation and 3D-printed models has grown. However, limitations of these are unknown and a research gap exists, with surface roughness being a major issue. Aim: To evaluate the surface roughness of clear silicon templates fabricated over 3D-printed models, blue inlay wax and dental stone. Materials and Methods: This in-vitro study utilised both quantitative and qualitative approaches. The study was conducted at the Department of Conservative Dentistry and Endodontics, Manubhai Patel Dental College and Hospital, Vadodara, Gujarat, India. The study was completed over three months. A total of 36 surfaces of clear silicone template (Exaclear) were obtained from two blocks each of 3D-printed model, blue inlay wax, and dental stone, measuring 30×10×10 mm. These blocks were divided into six units of 10×5 mm (N=36) and were divided into three groups: 1) 3D-printed model; 2) Blue inlay wax; 3) Dental stone. Surface roughness was evaluated using a surface roughness tester and Scanning Electron Microscope (SEM). Quantitative analysis of surface roughness was done using the surface roughness tester, and qualitative analysis was done using SEM. Statistical analysis was done using the posthoc Tukey Honest Significant Difference (HSD) test and statistical software SPSS Version 20.0. Results: The quantitative analysis showed the highest Roughness average (Ra) value mean±Standard Deviation (SD) for Group 1 (11.97±4.43 μm), followed by Group 3 (2.42±1.07 μm) and Group 2 (0.63±0.86 μm). SEM showed the presence of voids only in Group 1. Conclusion: Surface roughness of clear silicon template fabricated on wax surface is less as compared to templates fabricated on 3-D printed models.

Shaping Ability of Different Rotary and Reciprocating File Systems in Simulated S-Shaped Root Canals.

This study aimed to examine the shaping ability of six rotary and reciprocating file systems, as well as hybrid techniques in simulated S-shaped root canals. A hundred and twenty S-shaped radiopaque thermosetting epoxy resin blocks were grouped according to the system used for biomechanical preparation. Six groups of 15 canals each were prepared using TruNatomy, Procodile, VDW Rotate, Hyflex CM, OneCurve, and WaveOne Gold, respectively. Two additional hybrid (Procodile/Hyflex CM and Procodile/TruNatomy) groups (n=15 each) were added after evaluating the results of individual systems. All canals were enlarged to 0.25 mm apically. Canal transportation, centering ability, diameter increase, and iatrogenic errors were assessed by superimposed pre-operative and post-operative images. AutoCAD was used for data analysis. All groups were statistically compared with analysis of variance and Tukey honest significant difference test (p<0.05). Hyflex CM resulted in the most conservative diameter increase in all thirds (coronal, middle, and apical; p<0.001). Procodile showed the best (p<0.001) centering ability in the coronal and middle thirds, while TruNatomy resulted in the least canal transportation and most centering preparation in the apical third (p<0.001). Hybridisation of Procodile and Hyflex CM produced the least canal transportation and best centering ability in the middle third (p<0.001). No instrument breakage occurred, and no ledge, elbow, or apical zip formation was observed during canal preparation. Hybridisation of Procodile and Hyflex CM showed remarkable results in preserving the canal diameter in all thirds and resulted in the least canal transportation and best centering ability in the middle third of the canal. However, when treatment cost and duration limit the clinical applicability of the hybrid technique, clinicians can use a single file system (Hyflex CM or TruNatomy) since it shows satisfactory results in all parameters when compared with a hybrid system.

Evaluation of organizational culture in enterprises in the Czech Republic using OCAI.

The goal of this work was to use the Organizational Culture Assessment Instrument (OCAI) diagnostic method to find out the existing and preferred organizational culture in companies in the Czech Republic and then to evaluate their dependence on the size of the organizations. Data were collected in 2019-2021 and evaluated using Microsoft Excel and IBM SPSS 24 statistical program, e.g., descriptive statistics tools, one-sample z-test, analysis of variance and post hoc test (Tukey's honest significant difference - HSD). The research was conducted on a sample of 962 companies across the entire republic and fields. The results of the study show that clan culture prevails in the Czech Republic, which was dominant in all six dimensions. Enterprises in the Czech Republic have a mix of organizational cultures in the following order: (1) clan culture (31.72%), (2) hierarchical culture (25.46%), (3) market culture (21.5%), and (4) adhocratic culture (21.28%). However, with regard to the desired cultural mix, this order changes as follows: (1) clan (35.3%), (2) hierarchical (22.91%), (3) adhocratic (22.63%), and (4) market culture (19.17%). Furthermore, it was found that a statistically significant difference was observed in the assessment of organizational culture depending on the size of the organization in the Czech Republic. A limitation of the research could be the unequal number of respondents in 2020 and 2021, which does not allow comparing differences in the time period. This work can serve as a comparative basis of organizational culture with another national culture.

Long-term evaluation of soft-tissue changes after miniscrew-assisted and conventional rapid palatal expansion using voxel-based superimposition of cone-beam computed tomography scans

This study aimed to evaluate the soft-tissue changes in the long-term after miniscrew-assisted rapid palatal expansion (MARPE) and conventional rapid palatal expansion (RPE) appliances compared with a matched control group using voxel-based superimposition of cone-beam computed tomography (CBCT) scans. A total of 180 CBCTs for 60 patients at 3-time points were evaluated: pretreatment (T1), postexpansion (T2), and posttreatment (T3) for 3 groups: (1) MARPE, (2) RPE, and (3) controls (time-period T1 to T3: MARPE, 2 years 8 months; RPE, 2 years 9 months; control, 2 years 7 months). The voxel-based superimposition technique was used to superimpose the CBCT scans, after which the soft-tissue surfaces were extracted from the superimposed T1-CBCT, T2-CBCT, and T3-CBCT scans. Nine landmarks were identified on the CBCT scans: nasion, A-point, pogonion, right and left alar base, right and left zygoma, and right and left gonion. The coordinates of the 9 parameters were obtained in the x-axis, y-axis, and z-axis for the CBCT scans and subjected to statistical analyses. The changes in the soft-tissue surfaces were also evaluated by color-coded maps for short-term (T2) and long-term (T3) changes. The mean changes from T1 to T2 and T1 to T3 were tested against no change within the groups by paired t test, and the mean changes among the 3 groups were compared with analysis of variance F test with Tukey's Honest significant difference used for adjusting P values for multiple testing. In the short term, both MARPE and RPE led to a significant downward movement of pogonion, left gonion, and lateral movement of the right and left alar base compared with controls at T2 (P<0.05). In addition, MARPE led to a significant downward movement of right gonion than controls at T2 (P<0.05). Moreover, RPE led to a significant downward and forward movement of A-point and downward movement of the right and left alar base than controls at T2 (P<0.05). However, in the long-term, there were no significant differences in the soft-tissue changes among the MARPE, RPE, and control groups. MARPE and RPE do not lead to significant soft-tissue changes in the long term when compared with controls.

Evaluation of Physical Properties of Denture Base Resins Containing Silver Nanoparticles of Aloe barbadensis Miller, Morinda citrifolia, and Boesenbergia rotunda and Its Anti-microbial Effect: An In Vitro Study.

Introduction The denture bases fabricated from polymethylmethacrylate (PMMA) have some disadvantages, such as surface prone to microbial growth and biofilm accumulation, which contributes to the onset and dissemination of infections among denture wearers. Therefore, the purpose of this in vitro study was to evaluate the flexural strength, hardness, and antimicrobial effect of denture base resin incorporated with 0.05% and 0.1% silver nanoparticles (AgNPs) of Aloe barbadensis miller (aloe vera), Morinda citrifolia (noni), and Boesenbergia rotunda (finger root). Materials and methods A total of 84 PMMAsamples were used and were divided into three groups. Flexural strength tests were performed on Group 1 PMMAblocks. Group 2 involved hardness testing of PMMAblocks, whereas Group 3 involved antimicrobial activity. Each group was subsequently split into seven subgroups with differing concentrations of AgNPs: Sub Group 1: control (no AgNPs),Sub Group 2: 0.05% aloe vera AgNPs, Sub Group 3: 0.1% aloe vera AgNPs, Sub Group 4: 0.05% noni AgNPs, Sub Group 5: 0.1% of noni AgNPs,Sub Group 6: 0.05%finger root AgNPs, and Sub Group 7: 0.1% finger root AgNPs.The flexural strength was evaluated usinga universal testing machine (Instron 8801). Surface hardness was measured using a Vickers tester (Tukon 1102). For the antimicrobial activity analysis, the samples were incubated in a suitable culture broth containing Candida albicans for 24 hours. Microbial colony count (colony-forming unit (CFU)/mL) was estimated to evaluate the microbial adhesion to the surface of the denture base materials. Statistical analysis The flexural strength, hardness, and CFU between the groups were analyzed using one-way analysis of variance (ANOVA) followed by multiple comparisons with Tukey's honest significant difference (HSD) test (α=0.05). The level of statistical significance was determined at p<0.05. Results It was observed that the mean flexural strength was maximum in PMMA incorporated with 0.05% of aloe vera AgNPs and least in PMMA incorporated with 0.1% noni AgNPs. It was seen that a steady loss in flexural strength is observed from 0.05% to 0.1%. The mean hardness was maximum in PMMA incorporated with 0.1% of noni AgNPs and least in PMMA incorporated with 0.05% aloe vera AgNPs. It was also found that the hardness was directly proportional to the number of nanoparticles. With an increase in the weight percentage of nanoparticles, a steady increase in hardness was seen in all the test groups. In our study, the results showed that finger root 0.1% showed the least CFU with a significant reduction of C. albicans adherence; therefore, it indicates higher anti-fungal activity. Aloe vera 0.05% showed the lowest inhibition of C. albicans,suggesting the least anti-fungal activity. Conclusion Within the limitations of this study, It can thus be concluded that the addition of AgNPs incorporated with plant extracts of Aloe barbadensis miller (aloe vera), Morinda citrifolia (noni), and Boesenbergia rotunda (finger root) can alter the flexural strength, hardness, and microbial adhesion of PMMA. In our study, it can be concluded that flexural strength increases with the addition of AgNPs of 0.5% concentration after which a steady loss is seen. However, the hardness and antimicrobial activity increased with an increase in the concentration of AgNPs in all three plant extracts.

Kinetic analysis, mathematical modeling and quality evaluation of mint drying in greenhouse solar dryer

The goal of this study is to evaluate the potential of the Greenhouse Solar Dryer (GSD) for drying the mint leaves and compare it with other traditional drying methods. The top 10 mathematical models introduced by literatures were selected to describe the drying kinetics of mint leaves. The results showed that the average drying time in the GSD is 32 % and 50 % less than the open sun and shade place drying, respectively. Wang and Singh model with R2 = 0.9944, RMSE = 0.02021 and χ2 = 0.00052, logarithmic model with R2 = 0.9918, RMSE = 0.02556 and χ2 = 0.00078, and Henderson-Dabbie with model with R2 = 0.9774, RMSE = 0.04189 and χ2 = 0.00175 were determined as the best models for GSD, sun and shade place drying, respectively. The amount of changes in the color of the dried mint according to the index of total changes (ΔE) was recorded as 0.53 in the shade place, 0.60 in the GSD, and 0.89 in the open sun drying, respectively. The average of dried mint essential oil that was extracted by hydro-distillation method was 0.0254, 0.0237 and 0.0101 for shade place, GSD and open sun drying, respectively. Comparison of the average amount of extracted dried mint essential oil using ANOVA test, two LSD and Tukey HSD (Honestly Significant Difference) tests showed that there is no significant difference between the two treatments of drying in the shade place and GSD, while both of them have a significant difference with the open sun.

GALNT12 promotes fibrosarcoma growth by accelerating YAP1 nuclear localization.

Fibrosarcoma is a highly malignant type of soft tissue sarcoma that currently lacks effective treatment options. Polypeptide N-acetylgalactosaminyltransferase 12 (GALNT12) belongs to the uridine diphosphate N-acetylgalactosamine gene family, which is involved in numerous biological processes of diseases, such as tumor progression. Its upregulated expression is closely associated with the development of colorectal cancer. However, research on the role of GALNT12 in fibrosarcoma is currently limited. The present study aimed to assess the expression and biological function of GALNT12 in fibrosarcoma. Patient data and tissue samples were collected and public datasets were obtained from the Gene Expression Omnibus (GSE24369 and GSE21124). Immunofluorescence assays were performed to observe the cellular localization of GALNT12. GALNT12 expression was measured using reverse transcription-quantitative PCR, western blotting and immunohistochemistry. Small interfering RNAs were constructed to knock down GALNT12 expression in HT-1080 cells. Cell Counting Kit-8 and EdU assays were used to assess fibrosarcoma cell proliferation. Wound healing and Transwell assays were used to detect migration. Gene set enrichment analysis was performed to identify key pathways. Paired and unpaired Student's t-test, Fisher's exact test and one-way ANOVA (followed by Tukey's Honest Significant Difference test) were used to analyze the data. It was demonstrated that GALNT12 expression was upregulated in both fibrosarcoma cell lines and tissue samples and predicted poor patient prognosis. In vitro experiments demonstrated that high GALNT12 expression levels significantly increased HT-1080 cell proliferation and migration. Furthermore, it was demonstrated that high GALNT12 expression levels were closely associated with the yes1 associated transcriptional regulator (YAP1) signaling pathway. Knockdown of GALNT12 inhibited YAP1 nuclear translocation, which affected activation of key downstream genes including AMOTL2, BIRC5 and CYR61. Therefore, the present study demonstrated that GALNT12 promoted fibrosarcoma progression. GALNT12 could be a potential biomarker for this disease and may potentially provide new ideas for targeted therapy of fibrosarcoma in the future.

Evaluation and Assessment of the Colour Stability of Modified Polymethyl Methacrylate Denture Base Materials and Polyether Ether Ketone in a Cast Partial Denture Framework: An In-Vitro Study.

Background One of the frequent aesthetic issues patients confront is the loss of colour and lifeless appearance of the denturesof the base materials of their dentures after regular use. This leads to a lack of motivation to use the denture regularly. Due to the drawbacks of conventional PMMA, polyether ether ketone (PEEK) and newer polymethyl methacrylate (PMMA)-based materialshave now started being used in cast partial denture frameworks dueto their superior physical and biological properties. The lack of long-lasting colourisone of the main reasons for the repeat of dental prostheses. Hence, the need for the study is to help clinicians decide which would be the most suitable denture base materialto be used based on colour stability. Aim To assess and compare the colour stability of PEEK, polyan, and biodentaplast denture base materials (DBMs) upon staining withdistilled water, tea, coffee, and turmeric solutions after one day, seven days, and 30 days. Methods A total of 20 cuboidal specimens were constructed and immersed in distilled water, tea, coffee, and turmeric (five specimens of each material in each solution, a total of 60 specimens): Group 1: PEEK, Group 2: polyan, Group 3: biodentaplast. All specimens were subjected to colour measurements before exposure to beverage solutions, after 24 hours, on the seventh day and 30th day with a colour reflectance spectrophotometer with computer software. A one-way ANOVA test followed by post hoc Tukey's honestly significant difference (HSD) was performed for comparison of colour stability between the DBMs, revealing a significant difference between PEEK and polyan and PEEK and biodentaplast. Polyan showed the highest delta E values, followed by biodentaplast and PEEK.A two-way ANOVA test, followed by Tukey's HSD post hoc, was done to compare the staining ability of various staining solutions. Turmeric had the highest delta E values, followed by coffee, tea, and distilled water. Data were assessed using Statistical Product and Service Solutions (SPSS, version 23) (IBM SPSS Statistics for Windows, Armonk, NY) software. Results The highest mean delta E value at T1 was seen in biodentaplast immersed in turmeric (12.3900+/-0.442), and the least value at T1 was obtained for PEEK immersed in distilled water (0.4460+/-0.036).The highest mean delta E value at T2was seen in polyan immersed in turmeric (13.0160+/-0.28962), and the least value at T2was obtained for PEEK immersed in distilled water (0.5860+/-0.051). At T3, the highest mean delta E value was seen in polyan immersed in turmeric (16.8600+/-0.49845), and the least value at T3 was obtained for PEEK immersed in distilled water (0.700+/-0.037). Conclusion PEEK had the highest colour stability when compared with polyan and biodentaplast.

Near-infrared spectroscopy with a provocative maneuver to detect the presence of severe peripheral arterial disease

Current assessment standards for peripheral arterial disease (PAD), such as the ankle brachial index, are limited in their utility and portability. Near-infrared spectroscopy (NIRS) has shown some promise in diagnosing PAD when used in conjunction with a provocative maneuver. The purpose of this study was to assess the viability of NIRS in conjunction with a transient leg elevation provocative maneuver for detecting severe PAD. This retrospective observational cross-sectional study assessed 57 limbs in 34 patients receiving routine vascular screening for PAD at Madigan Army Medical Center. The patient limbs were stratified into normal (n = 17), mild (n = 9), moderate (n = 16), and severe (n = 15) PAD groups based on the clinician assessments. Additionally, the patients were assessed with NIRS measurements taken with the patient in the supine position at rest and using a provocative leg raise maneuver of transient leg elevation of 45° for 60 seconds. The resting tissue oxygen saturation (StO2) and the change in StO2 (ΔStO2) from rest to elevation were recorded and compared between the PAD severity groups via independent measures analysis of variance with the Tukey honest significant difference post hoc test. The supine resting StO2 was not different between the normal (77.5% ± 7.7%), mild (72.5% ± 7.4%), moderate (72.0% ± 10.3%), and severe (74.2% ± 5.4%) PAD groups (P = .23). However, the ΔStO2 with transient leg elevation was significantly greater in the severe PAD group (−17.2% ± 6.0%) compared with the normal (−3.9% ± 4.8%), mild (−6.9% ± 4.7%), and moderate (−9.7% ± 5.2%) PAD groups (P < .002 for all). Similar results were observed in the changes in oxyhemoglobin and deoxyhemoglobin. The leg elevation protocol was also used for two patients before and after lower limb revascularization, which demonstrated that the ΔStO2 corresponded with the clinical assessment of PAD severity. Resting supine NIRS images were unable to detect any differences among normal and limbs with different PAD severity. However, NIRS imaging with 45° leg elevation for 60 seconds showed a significant difference between severe PAD compared healthy patients and those with mild to moderate PAD in a fast, precise, and accurate manner. These preliminary data support the use of NIRS and transient leg elevation as a tool to diagnose severe PAD but do not support the use of NIRS alone as a screening test for PAD. NIRS measurements with leg elevation might be a viable noninvasive, noncontact, and portable method of assessing severe PAD for home monitoring, in rural communities, and/or in standard clinical practice.

An assessment of the fixin tplo jig to generate effective compression using a transverse fracture model.

The objective of this study was to determine compressive loads that could be generated using a tibial plateau leveling osteotomy (TPLO) jig with a tensioned strand of 18-gauge stainless steel orthopedic wire in a simulated transverse fracture model. The wire was sequentially tensioned using heavy needle holders or an AO wire tightener. Recorded loads were subsequently compared to loads generated by applying a 3.5 mm limited contact-dynamic compression plate (LC-DCP) as a compression plate. Two segments of 2 cm diameter Delrin rod were placed in a testing apparatus and used to simulate a transverse fracture. A load cell was interposed between the two segments to measure the compressive loads generated during the application of the TPLO jig or the LC-DCP. Compression was generated by sequential tensioning a strand of 18-gauge wire secured through the base of the arms of the TPLO jig or by placing one or two load screws in the LC-DCP. Wires were tensioned using heavy needle holders or an AO wire tightener. Eight replicates of each construct were tested. Recorded loads were compared using a one-way repeated measures ANOVA and Tukey Honestly Significant Difference test. The wire being tensioned broke while attempting a second quarter rotation of the needle holders and when the crank handle of the AO wire tightener was advanced beyond two rotations. The mean + SD peak compressive loads recorded when tensioning the wire using the heavy needle holders and AO wire tightener was 148 ± 7 N and 217 ± 16 N, respectfully. The mean ± SD load recorded after placement of the first and second load screw in the LC-DCP was 131 ± 39 N and 296 ± 49 N, respectively. The compression generated by placing two load screws in the LC-DCP was superior to the compression generated using the jig. The maximum load recorded by tensioning the wire secured through the TPLO jig using the AO wire tightener was superior to the compression generated by placing a single load screw and tensioning the wire using needle holders. Our results demonstrate that the TPLO jig allows surgeons to compress transverse fractures or osteotomies effectively. Tensioning the AO wire tightener allows for sequential tensioning and generates superior compressive loads than tensioning wires with heavy needle holders.

Rootzone cooling, water productivity and economics of intensified vertical grown red butterhead lettuce (Lactuca sativa Linn)

To alleviate the effects of high temperatures and high air humidity to lowland tropical crop production, resulting to poor quality marketable produce, rootzone cooling systems were applied to vertical recirculating hydroponic–drip irrigated towers for lettuce production. Two treatments, ground heat exchanger (GHE) and water chiller cooling systems, were installed to lower the temperature of the nutrient solution supplied to the towers. Harvest periods were 20, 25 and 30 days after transplant (DAT). Data gathered were analyzed using combined analysis for the strip plot design and Tukey Honestly Significant Difference (HSD) test for the comparison among means. For 30-DAT, using the GHE and chiller rootzone cooling systems, the average daily electrical conductivity was 2.10 dS/m and 2.14 dS/m, the pH levels were 6.14 and 6.27; and dissolved oxygen were 5.28 mg/L and 6.06 mg/L, respectively. Lettuce harvested at 30DAT using the water chiller rootzone cooling method had the highest water consumption of 2.13L/plant while those lettuce harvested at 20DAT using the ground heat exchanger rootzone cooling method had the least water consumption of 1.16L/plant. The highest water productivity using the ground heat exchanger rootzone cooling method was 31.18 g/L at 25 DAT while using the water chiller rootzone cooling method the highest water productivity was 33.45 g/L at 20 DAT. The lettuce harvested at 30DAT planted at the top level of the towers had the highest yield (89.42 g), the most number of leaves (with a mean of 59.50), and the widest leaves (73.58 mm). Chiller rootzone cooling method at 30DAT and planted at the middle level of the hydroponic tower had the longest leaf with 129.17 mm. Economic analysis showed that the GHE rootzone cooled nutrient solution at 25DAT is most feasible with benefit to cost ratio of 1.73, average net income Php166,963.48, return on investment of 23.39%, and payback period of 0.81 years

Evaluation of the relationship between metallurgical properties and metal-ceramic bond characteristics of Co-Cr alloys manufactured by different techniques

Metal-ceramic restorations made from cobalt chromium (Co-Cr) alloy have been increasing, but studies on the effects of different manufacturing techniques on metal-ceramic interface characteristics and metal-ceramic bond strength are sparse. The purpose of this in vitro study was to examine the metal-ceramic interface and the metal-ceramic bond strength of a Co-Cr alloy produced by casting, milling, and selective laser melting (SLM) with or without thermal cycling. Co-Cr alloys were prepared by casting, milling, and SLM. Two different SLM devices were used. Ninety-six specimens (25×3×0.5mm) were manufactured. The structure of the oxidation surface of Co-Cr specimens was examined by scanning electron microscopy (SEM) and by X-ray fluorescence spectroscopy (XRFS). After porcelain application, selected specimens were thermal cycled, and the strength of the metal-ceramic bond was measured by the 3-point bend test. All specimens were analyzed for failure type with a stereomicroscope. The elemental composition and morphology of the metal-ceramic interface were examined by XRFS and SEM with energy-dispersive X-ray (EDX). The results of bond strength were analyzed using a 2-way analysis of variance (ANOVA) for manufacturing methods and testing conditions and the Tukey honest significant difference (HSD) test (α=.05). The main effect of the interactions of the testing condition and manufacturing method variables on the bond strength variable was not statistically significantly different. No significant differences were found among the testing conditions tested (P=.638). Significant differences were found among the manufacturing methods tested statistically (P<.001). Statistically significant differences were found in the CAD-CAM and CONSEPT LASER groups, the CAD-CAM and SLM LASER groups, the CAD-CAM and CAST groups, and the CAST-SLM LASER groups (P<.05). Differences were observed among the interface morphologies of casting, milling, and the two SLM groups. The bond strength between Co-Cr alloy and ceramic is affected by the manufacturing method. The metal-ceramic bond strength is independent of thermal cycling. The bond strength value in all groups was over 25MPa, which has been considered clinically acceptable. The interface metallurgical structures of Co-Cr alloys were affected by different manufacturing techniques.

Evaluation the Marginal Adaptation for the Bio C Repair and Other Root end Filling Material by Using Scanning Electron Microscope (A Comparative In Vitro Study)

АBSTRАCT:‏‏‏‏‏‏ Background: The ability of the retrograde filling material to adhere to dentin has a substantial impact on the success of endodontic procedures. the marginal adaptation of the rᴏᴏt end filling materials considered important factor, since it demonstrates the materials adaptation to the dentinal walls. Objective: Assess the marginal adaptation of Biᴏ-C reparative materials in contrast to MTA (АNGELUS) and Amalgam. Material and method: A total of thirty human maxillary central incisors teeth that each had a single root were chosen. To keep the root canal length at 15mm, the crown was resected. The teeth underwent endodontic treatment, along with the resection of their root ends and preparation of root-end cavities. On the basis of the type of filling, the teeth were categorized into one of three groups, as follows: (А) Bio-C repair (Bioceramic reparative material), (B) MTА АNGELUS, (C) Amalgam. teeth were sectioned in longitudinal manner, marginal gabs measured by the use of a scanning electron microscope. Data was statically analyzed using АNOVА and Tukey Honestly Significant Difference (Tukey's HSD). Results: Bio-C Repair material and MTA show better marginal adaptation with significant difference between them and amalgam, no significant different between Bio-C repair group and MTA group. Conclusion: marginal adaptation better for Bio-C repair in comparison to Amalgam and MTА АNGELUS

Uso de Plectranthus amboinicus en pollos y su efecto en parámetros productivos y económicos

This research was carried out at the Santa Inés Farm of the Faculty of Agricultural Sciences of the Universidad Técnica de Machala, El Oro Province, Ecuador. The aim was to evaluate the effect of Plectranthus amboinicus, commonly called oreganon, on the productive and economic parameters of Cobb 500 broilers. For the well–being of the birds, the management established for open house systems in the area was used. A Completely randomized design was applied, where 6 treatments were used, each with 4 Experimental Units of 10 birds, for a total of 240 chickens evaluated. The treatments arrangement was: T1 or control which was based on a commercial type basal diet, while, to the balanced diets of treatments T2, T3, T4 and T5, the dehydrated–ground leaf of P. amboinicus was added at 0.25, 0.50, 0.75 and 1.00%, respectively, in replacement of the Growth Promoter Antibiotic (GPA); the T6 or blank, which did not contain GPA and dehydrated P. amboinicus in the balanced mixture. The variables evaluated were live weight gain (LWG), feed consumption (FC), accumulated water consumption (AWC), feed conversion ratio (FCR), mortality (M), productive efficiency factor (PEF), kg of standing meat per m2, economic expenses and cost per kg of standing meat. For all the variables, an ANOVA was used, previous assumptions of normality and homogeneity, and to discriminate between the means, Tukey's honest significant difference (HSD) procedure was used, with a confidence level of 95%. All data were analyzed using the PROC GLM (General Linear Model) procedure of the SAS statistical package. The results showed that oreganón could works as a replacement alternative to the GPA in broilers, without harming the productive and economic parameters.

Characterizing Walking Behaviors in Aged Residential Care Using Accelerometry, With Comparison Across Care Levels, Cognitive Status, and Physical Function: Cross-Sectional Study.

Walking is important for maintaining physical and mental well-being in aged residential care (ARC). Walking behaviors are not well characterized in ARC due to inconsistencies in assessment methods and metrics as well as limited research regarding the impact of care environment, cognition, or physical function on these behaviors. It is recommended that walking behaviors in ARC are assessed using validated digital methods that can capture low volumes of walking activity. This study aims to characterize and compare accelerometry-derived walking behaviors in ARC residents across different care levels, cognitive abilities, and physical capacities. A total of 306 ARC residents were recruited from the Staying UpRight randomized controlled trial from 3 care levels: rest home (n=164), hospital (n=117), and dementia care (n=25). Participants' cognitive status was classified as mild (n=87), moderate (n=128), or severe impairment (n=61); physical function was classified as high-moderate (n=74) and low-very low (n=222) using the Montreal Cognitive Assessment and the Short Physical Performance Battery cutoff scores, respectively. To assess walking, participants wore an accelerometer (Axivity AX3; dimensions: 23×32.5×7.6 mm; weight: 11 g; sampling rate: 100 Hz; range: ±8 g; and memory: 512 MB) on their lower back for 7 days. Outcomes included volume (ie, daily time spent walking, steps, and bouts), pattern (ie, mean walking bout duration and alpha), and variability (of bout length) of walking. Analysis of covariance was used to assess differences in walking behaviors between groups categorized by level of care, cognition, or physical function while controlling for age and sex. Tukey honest significant difference tests for multiple comparisons were used to determine where significant differences occurred. The effect sizes of group differences were calculated using Hedges g (0.2-0.4: small, 0.5-0.7: medium, and 0.8: large). Dementia care residents showed greater volumes of walking (P<.001; Hedges g=1.0-2.0), with longer (P<.001; Hedges g=0.7-0.8), more variable (P=.008 vs hospital; P<.001 vs rest home; Hedges g=0.6-0.9) bouts compared to other care levels with a lower alpha score (vs hospital: P<.001; Hedges g=0.9, vs rest home: P=.004; Hedges g=0.8). Residents with severe cognitive impairment took longer (P<.001; Hedges g=0.5-0.6), more variable (P<.001; Hedges g=0.4-0.6) bouts, compared to those with mild and moderate cognitive impairment. Residents with low-very low physical function had lower walking volumes (total walk time and bouts per day: P<.001; steps per day: P=.005; Hedges g=0.4-0.5) and higher variability (P=.04; Hedges g=0.2) compared to those with high-moderate capacity. ARC residents across different levels of care, cognition, and physical function demonstrate different walking behaviors. However, ARC residents often present with varying levels of both cognitive and physical abilities, reflecting their complex multimorbid nature, which should be considered in further work. This work has demonstrated the importance of considering a nuanced framework of digital outcomes relating to volume, pattern, and variability of walking behaviors among ARC residents.

Effect of chlorhexidine and benzalkonium chloride on the long-term push-out bond strength of fiber posts.

Fiber posts are widely used in endodontically treated teeth with extensive loss of coronal structure. The purpose of this study was to investigate immediate and the long-term effects of chlorhexidine (CHX) and benzalkonium chloride (BAC) application, on the push-out bond strength of fiber posts. Sixty mandibular premolars were decoronated, and root canal treatment was performed. After post space preparation, the specimens were divided into three groups according to the post space-surface pretreatment (n = 20); no surface treatment (control group-Group 1), 2% CHX application (Group 2), and 1% BAC application (Group 3). A self-curing adhesive cement and an etch and rinse adhesive were used for the cementation of posts. Three sections (one cervical, one middle, and one apical) of 1 mm thickness were prepared from each specimen. A push-out test was performed immediately on the half of the specimen sections (n = 10). The other half of the specimen sections were subjected to 20.000 thermal cycles before applying the push-out test (n = 10). The failure mode of each specimen was observed under a stereomicroscope at ×40 magnification. The data were analyzed by one-way analysis of variance (ANOVA), Tukey Honestly significant difference (HSD), and Tamhane tests (P = 0.05). The cervical thirds displayed the highest, and the apical thirds showed the lowest values in all groups (P < 0.05), except the control-aged group (P = 0.554). The aged control groups' values were found to be significantly lower than the aged CHX and BAC groups (P < 0.001). Aging significantly reduced the bond strength values of specimens in control groups (P < 0.001). However, aging did not significantly affect the push-out bond strength values of CHX and BAC groups (P > 0.050). The failure types were adhesive between the post and cement (type 1) in all groups, except control-aged group (type 2). The application of 2% chlorhexidine or 1% BAC may be an essential step that can be taken to preserve the bond strength of fiber posts.

Effect of Er, Cr: YSGG, Nd: YAG, and diode laser against different photosensitizers on tensile and shear bond strength of bonded composite to caries affected dentin.

This study aimed to assess the impact of various cavity disinfection methods on the adhesive bond integrity of composite resin to caries-affected dentin (CAD). Additionally, it will evaluate the micro tensile bond strength (µTBS) of different dentin substrates [CAD and sound dentin (SD)] using various adhesive agents. The sample consisted of twenty human mandibular molars with sound dentin (SD) and eighty with CAD. All samples were positioned in a group of polyvinyl pipes with an internal diameter of 3 mm and were positioned perpendicularly up against the cementoenamel junction (CEJ). A total of 60 CAD samples (n=10) were used for shear bond strength (SBS) testing. CAD samples were disinfected with erbium chromium-doped yttrium, scandium, gallium and garnet (Er, Cr: YSGG) laser in group 1, Diode laser in group 2, neodymium-doped yttrium aluminum garnet (Nd: YAG) laser in group 3, riboflavin in group 4, curcumin in group 5, and chlorhexidine in group 6. Sixty CAD samples were treated with Scotchbond™ Etchant and Scotchbond™ multi-purpose primer and bonded with composite for SBS testing. On the twenty remaining CAD samples that did not undergo any type of disinfection, as well as the twenty samples that had sound dentin (SD), two different types of adhesive systems were used for micro tensile testing. For ten of each CAD and SD sample, 3c™ Adper™ Scotchbond™ multi-purpose adhesive was applied to the dentin surfaces. For the remaining ten CAD and SD samples, the All-Bond 2 adhesive system was used. The samples were prepared for µTBS testing. In all specimens, bond failure was assessed using a stereomicroscope. Analysis of variance (ANOVA) and Tukey Honestly Significant Difference (HSD) tests were used to compare the means of multiple groups, at a significance level of p<0.05. CAD disinfected with chlorhexidine (CHX) (17.19±1.02 MPa) exhibited the highest SBS values. Samples in group 5 disinfected with curcumin activated by photodynamic therapy (PDT) showed the lowest SBS (12.49±1.11 MPa). Scotchbond adhesive displayed comparable µTBS (p>0.05) when applied on CAD and SD. Moreover, All-Bond 2 adhesive, when applied on CAD, exhibited µTBS significantly lower than All-Bond 2 adhesive on SD (p<0.05). Analysis of debonded CAD surface after SBS showed that a cohesive type of failure was dominant in different experimental groups, followed by adhesive CONCLUSIONS: CAD disinfection with Er:Cr: YSGG, Diode Laser, and Riboflavin activated by photodynamic therapy have the potential to be used as an alternative to CHX for acceptable shear bond strength. The use of Adper™ Scotchbond™ multi-purpose adhesive on CAD and SD did not significantly compromise µTBS.

Biosynthesis of magnesium oxide and calcium carbonate nanoparticles using Moringa oleifera extract and their effectiveness on the growth, yield and photosynthetic performance of groundnut (Arachis hypogaea L.) genotypes

Small-scale crop production has been significantly impacted by the heavy price, limited supply, and frequent shortage of inorganic fertilisers, which is partly attributable to the Covid-19 pandemic outbreak and led to rising oil and food prices. Thus, integrating environmentally friendly agricultural practices that can improve crop productivity and advance the sustainability of agricultural cropping systems is critical.This study synthesized and characterised MgO and CaCO3Moringa oleifera nanoparticles and assessed their effects on groundnut genotypes. The effect of biosynthesized MgO and CaCO3 nanoparticles using Moringa oleifera extract on the growth and yield of groundnut genotypes exposed to different concentrations of 50, 100 and 200 mg/L was examined. The experiment was carried laid out in a 3 × 8 factorial completely randomized design (CRD) with eight replicates per treatment. Each plant was sprayed with 5 ml of the solution crystalline size of the MgO and CaCO3 nanoparticles 2.48 nm and 10.30 nm, respectively. Foliar application of nanoparticle treatments was applied weekly except for the negative control. The collected data were subjected to a two-way analysis of variance (ANOVA). Mean separations were done using Tukey's Honest Significant Difference (HSD) at P < 0.05.The findings demonstrated that foliar application of MgO and CaCO3 nanoparticles positively affected groundnut biomass production. The results further revealed that the concentration of 50 mg/L of MgO and 100 mg/L of CaCO3 considerably improved groundnut plant growth, yield, and nodulation in comparison with other treatments. There is a great deal of evidence signifying that foliar applications of 50 mg/L of MgO 100 mg/L CaCO3 contributed greatly to plant growth and crop production. Therefore, 50 mg/L of MgO and 100 mg/L CaCO3 nanoparticles foliar application could be recommended as nano-fertilisers application rate for groundnut production.

Cooperative Learning in Mathematics of Grade 7 Students of Calamba Bayside National High School

This study compared the effectiveness of individual, partner and group learning in enhancing the learning of Grade 7 students in Statistics, Number Sense and Linear Equation. An action plan that can improve their performances in Mathematics was developed. This study used the experimental research method on the 72 Grade 7 students of Calamba Bayside National High School. The statistical treatments applied were the mean, t-test and one-way Analysis of Variance (ANOVA) with Tukey's Honest Significant Difference post-hoc test. The study revealed that in Statistics, Number Sense, and Linear Equation pre-test, the students belonged to the beginning level. In the conducted post-test in Statistics, they landed in the beginning (individual), developing (partners), and approaching proficiency (group) levels. In Number Sense and Linear Equation, they were beginners in individual, partners and group learning. There were significant differences between the pretest and posttest scores in terms of individual, partner and group learning in Statistics. There were significant differences between the Grade 7 students’ pretest and posttest scores under partner learning as well as group learning in Number Sense. In Linear Equation, there were significant differences between pretest and posttest scores in Linear Equation in individual, partner and group learning. In Statistics and Number Sense, there were no significant differences among the posttest scores of the three groups of Grade 7 students in individual, partner and group learning. In Linear Equation, there was significant difference between the means of partner and group learning.