A study was carried out to analyse rainfall and temperature trends and variability in selected agro-ecological zones in Kitui County. Climate Hazards Group Infrared Precipitation with Stations (CHIRPS) rainfall dataset was used while temperature data was obtained from the Climatic Research Unit gridded Time Series (CRU TS) dataset. The results indicated that there was a non-significant decreasing trend (p<0.05) in average annual rainfall in all the four agro-ecological zones (p<0.05) for a 30-years period (1988-2018) in the study area. A decreasing trend in March-April-May (MAM) seasonal rainfall trend was reported in the arid and semi-arid agro-ecological zones while an increasing trend was recorded in the transitional and semi-humid zones. For the October-November-December (OND) seasonal rainfall, a non-significant decreasing trend was reported in all the four agro-ecological zones. Additionally, a higher annual rainfall variability was recorded in the drier (arid) and wetter (semi-humid) zones compared to that in semi-arid and transitional zones. Moreover, the study established that there was a spatial variation in both MAM and OND seasonal rainfall variability and that rainfall variability was higher in the OND seasonal rainfall than that of the MAM seasonal rainfall in all the agro-ecological zones. With reference to temperature trends, a statistically significant increasing trend in annual and OND seasonal average maximum and minimum temperatures was reported in all the four agro-ecological zones. Further, the study noted a non-significant increasing trend in maximum and minimum temperatures for the MAM season in all the agro-ecological zones. In regard to temperature variability, the study deduced that there was low temperature variability compared to rainfall variability in all the four agro-ecological zones. The study recommends that location-specific rainfall and temperature analysis should guide planning and implementation of adaptation strategies for effective response to climate variability. Keywords: Agro-ecological Zones, Mann-Kendall Test, Coefficient of Variation, CHIRPS, CRU TS DOI: 10.7176/JEES/12-12-05 Publication date: December 31 st 2022