Microanalysis systems benefit from various advantages, such as their portable size, low cost, high accuracy, and short measurement times, prompting the development of numerous “lab-on-a-chip” techniques in recent decades. Biochemical applications of these techniques often require the rapid mixing of different fluid samples. This study presents a novel mixing technique using a four-phase traveling-wave electrode array. Traveling-wave electrode arrays subjected to alternating current (AC) signals are arranged on both sides of the microchannel walls, creating an overall chaotic mixing mechanism for a short microchannel and a low amplitude applied AC voltage. Numerical analyses reveal that the symmetric/asymmetric circulation zone can be generated by switching the phase-shift arrangement of the electrodes. The symmetric/asymmetric circulation zone involves convective and diffusive mixing mechanisms. The electrolyte conductivity, frequency, channel width and depth, and average inlet velocity affect the mixing performance. When the traveling-wave electrode arrays are implemented with 3D structures, the mixing efficiency is enhanced with a maximum achievable mixing quality of 98%.
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