Abstract Lifetime-based phosphor thermometry has been applied in a wide variety of surface temperature measurement applications due to its relative ease of implementation and robustness to background interference when compared to other optical temperature measurement methods. It is often assumed that the technique is minimally intrusive if the thickness of the applied phosphor coating is < 20 µm. To evaluate this assumption, high-speed phosphor surface temperature and thermocouple measurements were performed on 4140 steel substrates installed in the cylinder head of an optically-accessible internal-combustion engine for four operating conditions. For phosphor thermometry measurements, four substrates were studied, each coated with a phosphor layer of different thickness ranging between 6 µm and 47 µm. The phosphor thermometry measured temperature swings during combustion were shown to be heavily impacted by the presence of the phosphor coatings, increasing by roughly a factor of 2 - 2.5 when increasing the thickness from 6 µm to 30 µm. A technique was implemented which utilizes the temperature data in combination with a heat conduction model to provide estimates of the temperature swing and heat transfer flux in the absence of the phosphor coating. It was shown that even the 6 µm phosphor coating could lead to an order of magnitude increase in the temperature swing relative to the uncoated 4140 steel substrate. Despite the intrusiveness of phosphor thermometry for the surface temperature measurements, reasonable agreement was demonstrated between heat flux estimates determined with the heat transfer modeling technique and those deduced from temperature-swing measurements using two different high-speed thermocouples. The results indicate that phosphor surface thermometry can be a reliable surface temperature and heat flux diagnostic for transient high heat flux environments, as long as proper care is taken to account for the impact of the phosphor layer on the measurement.&#xD;