Transformation Events Research Articles

Biomass and Yield in Solanum lycopersicum Expressing a Synthetic Photorespiration Pathway

Considerable interest exists in improving the efficiency of photosynthesis and photorespiration to increase crop yields that will address growing world food needs. The current study investigated whether a novel synthetic photorespiratory pathway demonstrated to reduce photorespiratory metabolic energy demands and increase plant vegetative biomass in model species could boost tomato crop yield, notably yield of marketable fruit. The tomato cultivar Moneymaker was transformed with a synthetic photorespiration pathway construct containing a Cucurbita maxima malate synthase (MS) gene and a Chlamydomonas reinhardtii glycolate dehydrogenase (CrGDH) gene targeted to the chloroplast, with a plastid glycolate-glycerate translocator 1 (PLGG1) hairpin interference construct targeting the native photorespiratory pathway. Plants of seven T2 generation lines from independent transformation events expressed the MS and CrGDH transgenes, while having at least 3-fold reduction of native PLGG1 expression relative to non-transformed Moneymaker. Plants from transformed lines were larger and exhibited up to 63% increased biomass in comparison with the Moneymaker control. The number of fruit was similarly greater (1.4- to 4.2-fold) in modified plants; however, average individual fruit weights were significantly less for all but one line. Among transformed lines, larger fruited lines had the lowest biomass, while the smaller fruited lines had the highest biomass. Values for VCmax and Jmax derived from CO2 assimilation curves were statistically similar or lower in transformants relative to the control. Metabolic profiles obtained from high-performance liquid chromatography mass spectrometry (HPLC-MS) analysis revealed increases in forms of inactivated auxin. Principal component analysis of metabolite profiles separated transformants into two groups distinguished by total biomass and fruit size. Observed increases in plant biomass in transformed lines suggests that energy savings were realized from reduced photorespiration but were directed toward vegetative growth. Further studies to balance vegetative vs. reproductive biomass and exploit higher fruit counts observed in transformants may be beneficial for enhanced fruit yield.

B Cell Differentiation and the Origin and Pathogenesis of Human B Cell Lymphomas.

Immunoglobulin (IG) gene remodeling by V(D)J recombination plays a central role in the generation of normal B cells, and somatic hypermutation and class switching of IG genes are key processes during antigen-driven B cell differentiation in the germinal center reaction. However, errors of these processes are involved in the development of B cell lymphomas. IG locus-associated translocations of proto-oncogenes are a hallmark of many B cell malignancies. Additional transforming events include inactivating mutations in various tumor suppressor genes and also latent infection of B cells with viruses, such as Epstein-Barr virus. Most B cell lymphomas require B cell antigen receptor expression, and in several instances chronic antigenic stimulation plays a role in lymphoma development and/or sustaining tumor growth. Often, survival and proliferation signals provided by other cells in the microenvironment are a further critical factor in lymphoma development and pathophysiology. Most B cell malignancies derive from germinal center B cells, most likely due to the high proliferative activity of these B cells and aberrant mutations caused by their naturally active mutagenic processes.

Host-Induced Gene Silencing of the Aspergillus flavus O-Methyl Transferase Gene Enhanced Maize Aflatoxin Resistance.

Maize is one of the major crops that are susceptible to Aspergillus flavus infection and subsequent aflatoxin contamination, which poses a serious health threat to humans and domestic animals. Here, an RNA interference (RNAi) approach called Host-Induced Gene Silencing (HIGS) was employed to suppress the O-methyl transferase gene (omtA, also called aflP), a key gene involved in aflatoxin biosynthesis. An RNAi vector carrying part of the omtA gene was introduced into the B104 maize line. Among the six transformation events that were positive for containing the omtA transgene, OmtA-6 and OmtA-10 were self-pollinated from T1 to T4, and OmtA-7 and OmtA-12 to the T6 generation. These four lines showed at least an 81.3% reduction in aflatoxin accumulation at the T3 generation under laboratory conditions. When screened under field conditions with artificial inoculation, OmtA-7 at T5 and T6 generations and OmtA-10 at T4 generation showed a reduction in aflatoxin contamination between 60% and 91% (p < 0.02 to p < 0.002). In order to develop commercial maize lines with enhanced aflatoxin resistance, the omtA transgene in OmtA-7 was introduced into three elite inbred lines through crossing, and the resulting crosses also exhibited significantly lower aflatoxin accumulation compared to crosses with non-transgenic controls (p < 0.04). In addition, high levels of omtA-specific small RNAs were only detected in the transgenic kernel and leaf tissues. These results demonstrate that suppression of omtA through HIGS can enhance maize resistance to aflatoxin contamination, and this resistance can be transferred to elite backgrounds, providing a viable and practical approach to reduce aflatoxin contamination in maize.

Association between GPX4 and HMGCR gene expression and cell proliferation in atypical hyperplasia of the breast

Aim: Using a dataset available from the NCBI Gene Expression Omnibus Repository, this in silico study investigated the differential expression of GPX4, the gene coding for the detoxifying enzyme glutathione peroxidase 4, in paired samples of AH (atypical hyperplasia) and corresponding histologically normal (HN) tissue from 17 women with AH and in four samples of normal breast tissue used as controls. Methods: The study focused on the genes (HMGCR, FDPS, FDFT1, and GGPS1) involved in the production and breakdown of isopentenyl-diphosphate, a key component for GPX4 biosynthesis. It also explored the connection between the expression of GPX4 and the genes (CCND1, CDK4, CDK6, and CDKN1B) associated with the cyclin D1-CDK4/6 complex. Results: Compared to HN tissue, AHs exhibited higher levels of GPX4 and HMGCR, supporting the functional connection between GPX4 synthesis and isopentenyl-diphosphate production. Additionally, AHs showed elevated levels of CCND1 and CDKN1B and decreased levels of CDK6. Compared to normal breast tissue, HNs showed similar alterations, suggesting that ferroptosis escape and uncontrolled proliferation are early molecular events in the neoplastic transformation. Compared to HN tissue, AHs also expressed high levels of GGPS1, a downstream gene of HMGCR, which leads to the synthesis of geranylgeranyl-diphosphate, a molecule essential for the post-translational modification of the proteins involved in the regulation of the Hippo signaling pathway. Conclusions: Although very preliminary, present results seem to suggest that blocking the mevalonate pathway by statins might, on the one hand, prevent AHs from escaping ferroptosis through depleting isopentenyl-diphosphate and, on the other hand, inhibit cell proliferation by controlling the Hippo pathway.

The Influence of Historical Events on Victorian Literature

Victorian literature serves as a rich reflection of the 19th century’s transformative historical events, including the Industrial Revolution, the expansion of the British Empire, social reform movements, and challenges to traditional religious beliefs. This article explores how authors such as Charles Dickens, Elizabeth Gaskell, Rudyard Kipling, Charlotte Brontë, Thomas Hardy, Alfred Lord Tennyson, and Matthew Arnold addressed themes of industrialization, colonialism, class conflict, and spiritual doubt. Through their works, Victorian writers critiqued societal norms, highlighted inequalities, and grappled with the shifting moral and intellectual landscape of their time. Their literature remains a vital lens through which to understand the complexities of the era and its enduring relevance today.

Adjuvant rituximab and elevated intratumoural CD8 expression are associated with sustained disease control after radiotherapy in a randomised trial of systemic therapy in early-stage follicular lymphoma

We report extended follow-up of TROG99.03, a randomised phase III trial in early-stage follicular lymphoma (ESFL) including new information on the role of adjuvant rituximab and translational studies. Patients with ESFL were randomised to involved field radiotherapy (IFRT) or IFRT plus 6-cycles cyclophosphamide/vincristine/prednisolone (IFRT+CVP). From 2006 rituximab was added to IFRT+CVP (IFRT+R-CVP). Clinical and multi-omic parameters were evaluated. Findings were validated in two independent ESFL cohorts (99 and 60 patients respectively). Between 2000 and 2012, 150 (75 per arm) patients were recruited. 48% were positron emission tomography (PET)-staged. By protocol, at median follow-up 11.3-years, progression-free survival (PFS) remained superior for IFRT+(R)CVP vs. IFRT (hazard ratio [HR]=0.60, 95% CI=0.37-0.98, p=0.043; 10-year PFS 62% vs. 43%) respectively. Although no significant difference in overall survival was observed (HR=0.44, 95% CI=0.16-1.18, p=0.11, 10-year OS 95% vs. 84%), patients receiving IFRT+(R)CVP experienced fewer composite (histological transformation and death) events (p=0.045). PFS of IFRT+R-CVP-treated patients compared with all other treatments lacking rituximab (IFRT alone plus IFRT+CVP) was superior (HR=0.36, 95% CI=0.13-0.82, p=0.013). Amongst PET-staged patients, PFS differences between IFRT+R-CVP vs. IFRT were maintained (HR=0.38, 95% CI=0.16-0.89, p=0.027) indicating benefit distinct from stage migration. FL-related mutations and BCL2-translocations were not associated with PFS. However, by multivariate analysis elevated CD8A gene expression in diagnostic biopsy tissue was independently associated with improved PFS (HR=0.45, 95% CI=0.26-0.79, p=0.037), a finding confirmed in both ESFL validation cohorts. CD8A gene expression was raised (p=0.02) and CD8+ T-cell density higher within follicles in ESFL vs. advanced-stage FL (p=0.047). Human leucocyte antigen class I specific neoantigens were detected in 43% of patients, suggesting neoantigen-specific CD8+ T-cells have a role in confining the spread of the disease. Adjuvant R-CVP and elevated intratumoural CD8 expression were independently associated with sustained disease control after radiotherapy in ESFL. Cancer Council Victora; National Health and Medical Research Council; Leukaemia Foundation; Mater Foundation.

Knowledge sourcing strategy and radical innovative performance: A temporal approach

Prior research on open innovation has so far taken a static view, by focusing on stable patterns of external knowledge sourcing that only depict a snapshot of a firm's external search at a given point in time. Such a static view, however, ignores the fact that a firm's portfolio of external knowledge sources evolves over time. Our study attempts to address this gap by clarifying how temporal variations in a firm's knowledge sourcing activities (i.e., changes in external search breadth and external search depth) affect its radical innovative performance. We conceptualize temporal variations as encompassing simple change events (i.e., single transformative events captured through increases or decreases in breadth and depth) and multiple change events (i.e., repeated changes captured through increases and/or decreases in breadth and depth); and then develop theoretical arguments that draw on the organization learning theory. We find that asymmetries emerge when comparing simple and multiple change events in external breadth and external depth. Our findings contribute to prior research on open innovation by providing insights into the temporal dynamics and evolution of knowledge sourcing activities.

White LED intensities during co-cultivation affect the Agrobacterium-mediated soybean (Glycine max) transformation using mature half seeds as explants.

The transition of light fixture from fluorescent light to light-emitting diodes (LEDs) in growth chambers prompts a reevaluation of current practices in plant biotechnology. Agrobacterium-mediated transformation is crucial for genetic engineering and genome editing in soybean (Glycine max). The critical co-cultivation step of soybean transformation occurs under light condition. Current protocols for co-cultivation in soybean transformation lack a standard for light intensity. In the present study, the objective is to investigate the effect of light intensity during co-cultivation on soybean transformation efficiency. Five light intensities were implemented during five days of co-cultivation: 50, 100, 150, 190 μmol∙m-2∙s-1 of white LEDs in addition to 100 μmol∙m-2∙s-1 of fluorescent light. After co-cultivation, all the explants underwent shoot induction and elongation with selection pressure, rooting and acclimation under uniform condition. The experiment was conducted with two selectable markers, hppdPf-4Pa and bar, separately, investigating whether the potential light effects vary due to the marker-associated pathways. The positive PCR analysis of rooted in vitro plants suggested successful transformation events achieved under both selectable markers across all light treatments ranging from 2.4% to 6.9%. Increasing LED light intensity during co-cultivation resulted in different transformation efficiencies between the two selectable markers. Results indicated that increasing the light intensity during co-cultivation led to a linear increase in transformation efficiency when shoot regeneration was under 4-Hydroxyphenylpyruvate dioxygenase (HPPD) inhibitor selection. No difference in transformation efficiency was detected among the treatments under glufosinate selection. Furthermore, when selection occurred with HPPD inhibitor, variation of transformation efficiency was also observed between fluorescent light and white LED at 100 μmol∙m-2∙s-1. The results highlight the significance and potential applications of investigating the impact of light on transformation efficiency.

Over expression of modified Isomaltulose Synthase Gene II (ImSyGII) under single and double promoters drive unprecedented sugar contents in sugarcane.

Sugarcane has been grown all around the world to meet sugar demands for industrial sector. The current sugar recovery percentage in sugarcane cultivars is dismally low which demands scientific efforts for improvements. Multiple approaches were adopted to enhance sugar contents in commercial sugarcane plants in contrast to conventional plant breeding methods. The exploitation of biotechnological methods and exploration of isomaltulose synthetic genes presented a promising solution to increase the existing low level of sugar recovery percentage in Saccharum officinarum L. Isomaltulose synthase gene II was employed and integrated into plant expression vector driven under the leaf and stem specific promoters terminated by nopaline synthase gene in a cloning strategy shown in the present study. Three gene constructs were developed in various combinations driven under promoters Zea mays ubiquitin and Cestrum Yellow Leaf Curl virus in the single and double combined stacked system. The transformation was executed in multiple formats with single transformed events, double promoter transformation events and triple construct stacked promoters in sugarcane induced calli via the particle gene gun. The transformation of ImSyGII in sugarcane genotype HSF-240 was confirmed by molecular gene analysis while expression quantification was determined through Real Time PCR. Furthermore, HPLC was also done to harvest the increased amounts of Isomaltulose in transgenic sugarcane juice. The present work upheld the enhanced ImSyGII expression in leaves owing to the exploitation of ubiquitin, while the Cestrum Yellow Leaf Curl virus promoter enhanced gene expression in sugarcane stems. The employment of three gene constructs collectively produced elite sugar lines producing more than 78% enhancements in whole sugar recovery percentage. The mature internode proved highly efficient and receptive regarding the production of isomaltulose. Quantifications and sugar contents evaluations upheld an increased Brix ratio of transgenic sugarcane lines than control lines.

Synthesis and biological evaluation of a new class of azole urea compounds as Akt inhibitors with promising anticancer activity in pancreatic cancer models

The PI3K/Akt pathway is crucial in numerous cellular functions such as cell growth, survival proliferation and movement in both normal and cancer cells. It plays also a key role in epithelial-mesenchymal transitions and angiogenesis during the tumorigenesis processes. Since many transformative events in cancer are driven by increased PI3K/Akt pathway signaling, Akt is considered a valuable target for developing new therapies against various tumor types, including pancreatic cancer. This is because the PI3K/AKT/mTOR pathway is a key downstream effector of RAS, and RAS activation is the most prominent genetic alteration in pancreatic cancer. Herein we report the synthesis and the biological evaluation of a new series of azole urea compounds that exhibited promising antiproliferative and antimigratory activities against pancreatic cancer cells through an Akt inhibition mechanism. These effects were demonstrated using a variety of assays, including Sulforhodamine B, cell-cycle, wound-healing, and kinase activity, apotposis and ELISA assays. Additionally, the anticancer properties of the most active compound in the series were confirmed in the 3D spheroid model of PATU-T cells.

Evolutionary Perspectives on Human-Artificial Intelligence Convergence.

In this analytical review, we explore the potential impact of the rapid proliferation of artificial intelligence (AI) tools on the biosphere and noosphere, suggesting that the trend may lead to a transformative event that could be termed "Human-AI integration." We argue that this integration could give rise to novel lifeforms, associations, and hierarchies, resulting in competitive advantages and increased complexity of structural organizations within both the biosphere and noosphere. Our central premise emphasizes the importance of human-AI integration as a global adaptive response crucial for our civilization's survival amidst a rapidly changing environment. The convergence may initially manifest itself through symbiotic, endosymbiotic, or other mutualistic relationships, such as domestication, contingent on the rate at which AI systems achieve autonomy and develop survival instincts akin to those of biological organisms. We investigate potential drivers of these scenarios, addressing the ethical and existential challenges arising from the AI-driven transformation of the biosphere and noosphere, and considering potential trade-offs. Additionally, we discuss the application of complexity and the balance between competition and cooperation to better comprehend and navigate these transformative scenarios.

Cyclin-D1 rearrangement as a secondary event in the large cell transformation of splenic marginal zone lymphoma with a TP53 deletion.

IGH::CCND1 translocation has been implicated as a preliminary finding in mantle cell lymphoma (MCL) and plasma cell myeloma, with cyclin D1 over-expression by immunohistochemistry stain, without gene rearrangement, reported in other hematologic neoplasms. CCND1 rearrangement has been reported infrequently as a secondary event in high-grade B-cell lymphomas. We present the case of an elderly man who was found to have a splenic marginal zone lymphoma with a heterozygous TP53 deletion. Years later at the time of relapse, the patient acquired a CCND1 rearrangement, in addition to apersistent TP53 deletion. Sequencing of the two lymphomas demonstrated clonal relatedness of the two processes. To our knowledge, this is the first report of a splenic marginal zone lymphoma witha TP53 deletion at diagnosis, evolving into a large B-cell lymphoma with a CCND1 rearrangement.

Paradox of Wars in Indian Writings: Traversing The Unintended Path to Peace

This paper is an exploration as to why wars become significant and important through historical journey to present times. Events that happened in the deepest of deep pasts continue to fascinate and attract humanity from around the globe. Continuous interpretations and re interpretations of the great Epics and other revered Indian texts have helped in preserving both the religious and cultural identity of not only the great Indian Sub Continent but the whole region that comes under South Asia. Wars under discussion are more than physical confrontations. Be it cosmic or human warfare, it serves to emphasize upon the inter connectedness that exists between the material realm and the spiritual one. Wars have cathartic effect on individuals. Examples of this can be seen in literary texts, historical texts and those penned down by great philosophers. These transformative events bring psychological release for individuals. There is emotional purification both at individual and collective level of societies. Aristotle says that, “Tragedy is an imitation of a serious and complete action, having magnitude, which through pity and fear bring about a catharsis of such emotions”. Catharsis comes with different explanations. The events arousing ‘cathartic’ effect mean that they are fully clarified for their role as being essential and with universal significance. The narrators of tragic events are probably meant to make readers move from particular to the general by making ‘reader spectator’ come face to face with what is known as the ‘universal law’.

Dialectic of the Event

This article aims to compare Gert Biesta’s, Hartmut Rosa’s and Thomas Kuhn’s perspectives on how transformative “events” emerge and become possible, and to relate their viewpoints to an educational context. The article shows how Biesta’s concept of subjectification, and Rosa’s idea of resonance, depend on viewing contemporary education as a state of status quo that is disrupted by a transformative event. Although their diagnoses of the contemporary and views on individual agency differ to some extent, Biesta and Rosa respectively place emphasis on the relationship between the subject and the world as the “space” where these events emerge. Subsequently, the article turns to Kuhn to propose another approach to understanding the dialectic of status quo and events. Through Kuhn, it is possible to introduce a more affirmative way of looking at how a state of status quo can underpin events, and how these events take place on a collective level, rather than in encounters between individual subjects and the world.

"Evolving Horizons: The Journey And Impact Of LIC's Landmark IPO"

The Initial Public Offering (IPO) of the Life Insurance Corporation of India (LIC) in May 2022 marked a pivotal transition for one of India’s largest financial institutions from a state-owned entity to a publicly listed company. This move was part of the government's broader strategy to divest its stakes in public sector enterprises, aiming to enhance operational efficiencies, improve transparency, and maximise shareholder value. Navigating through a series of legislative amendments and complex valuation challenges, the IPO drew substantial attention from both domestic and international investors, reflecting strong confidence in LIC's market fundamentals. This paper provides a detailed review of the IPO process, the subsequent market response, and its impact on LIC’s business operations and governance structure. It discusses the strategic motivations behind the IPO, the execution challenges, and the implications for future economic reforms in India. By analysing LIC’s performance post-IPO, the study highlights the critical role such transformative events play in modernising state-owned enterprises and integrating them with global financial markets, projecting significant implications for similarentities considering public offerings.

Refinement and Enhancement of Agrobacterium-Mediated Transient Transformation for Functional Gene Examination in Mulberry (Morus L.)

Background: Mulberry (Morus L.), a vital perennial woody plant with significant economic importance, is utilized for silkworm rearing, human consumption and medicinal use. The availability of mulberry’s whole-genome sequencing data has underscored the demand for an effective, user-friendly, and high-throughput protocol to facilitate the elucidation of gene functions. Methods and Results: In this investigation, we established a transient transformation approach using Agrobacterium tumefaciens-mediated sonication followed by vacuum infiltration in mulberry tissue culture seedlings. Simultaneously, we optimized the transformation conditions, including mulberry genotypes, A. tumefaciens strain, acetosyringone concentration, bacterial density, sonication time, and days after agroinfiltration. These optimizations aimed to achieve heightened transformation efficiency, employing GFP as a reporter gene to monitor transformation events. The optimized method included the use of an infiltration medium (10 mM MgCl2, 10 mM MES (2-(N-morpholino)ethanesulfonic acid sodium salt), 150 μM acetosyringone, and OD600 0.5 of A. tumefaciens LBA4404) supplemented with the surfactant 0.02% Silwet L-77, with 20 s sonication followed by 20 min vacuum infiltration (0.07 MPa). Among the four mulberry genotypes, ‘Taiguo’ was the most responsive genotype and produced the highest levels of GFP expression at 7 d after infiltration. Furthermore, the optimized transient transformation approach has been proven to be successfully applicable for transiently overexpressing MaANS and MaDFR in mulberry fruits of ‘Taiguo’, in vitro, which distinctly enhanced fruit coloring and significantly increased anthocyanin accumulation, respectively. Conclusions: In summary, we devised a dependable, stable and highly efficient transient transformation approach suitable for rapid gene function examination in mulberry leaves and fruits, in vitro.

Events, turns, and critical junctures: unpacking the temporality of supporter rights and democracy in English football

ABSTRACT The future of professional men’s football in England stands at a critical juncture. In the wake of a global pandemic, the national game has been beset by volatility and upheaval. In April 2021, six of the most storied clubs in England announced they would be joining a new breakaway European Super League (ESL). These proposals triggered vehement opposition from fan movements, catalysing the intervention of the UK government who established a fan-led review of football governance (FLR) to consult fans on future regulatory reform and the security and safety of supporters at major events. Emerging out of these exogenous shocks and endogenous change, the governance of English football is set to be radically transformed via the establishment of a new regulatory regime. In this article, we employ eventful sociology and historical institutionalism to operationalise the concept of critical juncture and apply this through an analysis of two temporal periods (1985-1990) and (2020-2024). In doing so, we show how the current ‘regulatory turn’ is interdependently linked to prior historical transformative events, namely the Heysel and Hillsborough stadium disasters and the subsequent neoliberal transformation of English football across the last four decades. Incorporating a more nuanced understanding of change and continuity in the governance of English football, we argue that new regulatory frameworks, despite their transformative potential, may continue to exhibit institutional legacies of the existing regime and prevailing political economy.

Categories as learning practice: navigating contested belonging along transatlantic mobile trajectories

ABSTRACT Although mobility-related categorization processes are central to migration studies, the ways that mobile populations understand, adapt, or contest them remain understudied. To trace such interpretations across both space and time, this paper explores a migrant trajectory that first crossed national borders within Africa before continuing to Brazil and later proceeding to Canada. The research combines ethnographic insights with the autobiographic reflections of one protagonist, whose perspectives and experiences move between different places, countries, institutions, people, and critical events. Following that individual’s learning processes, this article traces which categories were meaningful in the context of origin, how these changed in the interaction with different authorities, how transformative events played into valorizations, and which signs of categorical dissolution were recognizable during these trajectories. A biographical learning perspective sees not only the aspirations and the ideals but also the pragmatism and skepticism around the impact of mobility-related categories change along such journeys.

A new theorization of non-binary urban geographies: introducing the ‘urban grotesque’

ABSTRACT This article introduces the ‘urban grotesque’ as a novel theoretical lens to explore non-binary urban representations, using the Lokmacı/Ledra Street Buffer Zone in Nicosia, Cyprus, as a case study. Situated in a city divided by conflict, the Buffer Zone serves as a living laboratory for examining the complex interplay of memory, identity, and place beyond traditional binary frameworks. Through a rich tapestry of empirical observations and qualitative urban ethnography, the study traces the Buffer Zone’s transformation from a symbol of division to a vibrant place of non-binary urban representation. It highlights transformative events and movements that challenge binary norms and foster a sense of belonging and inclusivity, illustrating the fluid nature of urban places shaped by an array of social practices and experiences. The ‘urban grotesque’, inspired by Bakhtin’s conception of transformation and ambivalence, emerges as a pivotal framework for understanding the essence of non-binary urban geographies. This perspective not only bridges the theoretical and empirical but also enriches our perception of urban places as realms of endless becoming, inviting us to see beyond dichotomies. Ultimately, this study promotes an inclusive, dynamic comprehension of urban places in conflict zones, highlighting non-binary perspectives to enrich urban environments.

Manipulation of natural transformation by AbaR-type islands promotes fixation of antibiotic resistance in Acinetobacter baumannii

The opportunistic pathogen Acinetobacter baumannii, carries variants of A. baumannii resistance islands (AbaR)-type genomic islands conferring multidrug resistance. Their pervasiveness in the species has remained enigmatic. The dissemination of AbaRs is intricately linked to their horizontal transfer via natural transformation, a process through which bacteria can import and recombine exogenous DNA, effecting allelic recombination, genetic acquisition, and deletion. In experimental populations of the closely related pathogenic Acinetobacter nosocomialis, we quantified the rates at which these natural transformation events occur between individuals. When integrated into a model of population dynamics, they lead to the swift removal of AbaRs from the population, contrasting with the high prevalence of AbaRs in genomes. Yet, genomic analyses show that nearly all AbaRs specifically disrupt comM, a gene encoding a helicase critical for natural transformation. We found that such disruption impedes gene acquisition, and deletion, while moderately impacting acquisition of single nucleotide polymorphism. A mathematical evolutionary model demonstrates that AbaRs inserted into comM gain a selective advantage over AbaRs inserted in sites that do not inhibit or completely inhibit transformation, in line with the genomic observations. The persistence of AbaRs can be ascribed to their integration into a specific gene, diminishing the likelihood of their removal from the bacterial genome. This integration preserves the acquisition and elimination of alleles, enabling the host bacterium-and thus its AbaR-to adapt to unpredictable environments and persist over the long term. This work underscores how manipulation of natural transformation by mobile genetic elements can drive the prevalence of multidrug resistance.