Background/ObjectivesThis study aimed to compare the effects of two combined training methods on health-related physical fitness components in adolescents. MethodsSeventy-six adolescents (16.1 ± 1.1 years, n = 44 female) were randomized into groups of moderate-intensity continuous training combined with resistance training (MICT + RT), high-intensity interval training combined with resistance training (HIIT + RT), or Control. The training sessions were performed twice weekly for 12 weeks. The health-related physical fitness components evaluated were: body composition, muscular and cardiorespiratory fitness. ResultsThe intervention groups had a significant reduction in body fat percentage and improvement in abdominal repetitions and VO2peak after 12 weeks of combined exercise program (p < 0.001). Intervention effects were found to significantly reduce body fat percentage for the MICT + RT and HIIT + RT groups compared to the control group (mean difference: −3.8; 95% CI: −6.2; −1 0.3; mean difference: −4.7; 95%CI: −7.1; −2.3, respectively). For muscle fitness, significant effects of the intervention were found in increasing the number of abdominal repetitions favoring the MICT + RT group compared to the control group (mean difference: 9.5; 95% CI: 4.4; 14.7) and HIIT + RT compared to the control group (mean difference: 14.1; 95% CI 9; 19.3). For cardiorespiratory fitness, significant effects of the intervention on improving VO2peak were found in the experimental groups (MICT + RT vs Control group: mean difference: 4.4; 95% CI: 2.2; 6.6; and HIIT + RT vs. Control group: mean difference: 5.5, 95% CI: 3.3; 7.7). ConclusionThe results suggest that 12 weeks of training using MCIT + RT or HIIT + RT showed a similar effect for health-related physical fitness components in adolescents.