Tract Of Dogs Research Articles

Effects of trastuzumab emtansine on canine urothelial carcinoma cells in vitro and in vivo.

Urothelial carcinoma (UC) is the most common malignancy of the urinary tract in dogs and has aggressive behaviour. Although human epidermal growth factor receptor 2 (HER2) is a known therapeutic target with evidence in canine UC, the efficacy of anti-HER2 antibody drugs remains unknown. This study aimed to investigate the effects of anti-HER2 antibody drugs including trastuzumab and trastuzumab emtansine (T-DM1) on canine UC cell lines in vitro and in vivo. Four canine UC cell lines (Nene, TCCUB, Love, and Sora) were used. In western blotting, HER2 protein expression was observed in all the cell lines. Although both trastuzumab and T-DM1 showed dose-dependent growth inhibitory activity in the cell lines, T-DM1 showed much stronger activity than that of trastuzumab. In flow cytometry analyses with the canine UC cell line (Sora), T-DM1 but not trastuzumab significantly increased the percentages of early and late apoptotic cells in annexin V apoptotic assays and the sub-G1 phase fraction in cell cycle analyses. For the in vivo experiment, the canine UC cells (Sora) were subcutaneously injected into nude mice. Four days after inoculation, trastuzumab, T-DM1, or vehicle was administered intraperitoneally once a week for three times. Tumour volumes were significantly smaller in the T-DM1 group compared to the trastuzumab and vehicle control groups. These findings indicate that T-DM1 exerts a stronger antitumour effect than that of trastuzumab on canine UC cells in vitro and in vivo, possibly by inducing apoptosis due to DM1.

Ultrasonography of the Urinary Tract in Dogs and Cats : Clinical Investigations & Prevalence

Ultrasonography of the Urinary Tract in Dogs and Cats : Clinical Investigations & Prevalence

Examination of urine microflora and resistance of isolated pathogens during inflammatory processes of the urinary tract in dogs

Antibiotic-resistant bacteria are currently frequently isolated from pets and farm animals. The long-term irrational use of antibiotics for the treatment of animals and humans is underestimated and requires further attention and research, including in Ukraine. The aim of our study was to identify bacterial isolates and study their sensitivity to antibiotics in urinary tract inflammation in dogs. According to the statistical data of the VetForce system of the BTNAU clinic, out of 202 dogs examined in the clinic, 15 (7.43 %) were diagnosed with diseases with signs of urinary tract inflammation. It has been established that a large number of microorganisms, mainly E. coli, Streptococcus urinae, Pseudomonas aeruginosa and Staphylococcus aureus, causes inflammatory processes of the urinary tract in dogs. A less common microorganism, Klebsiella pneumonia, proved to be resistant. It was found that bacterial cystitis (the first group of animals) prevailed 1.5 times more often than in animals with urolithiasis. In dogs of the first group of 6–12 years old and over 12 years old, the percentage of the disease was higher compared to the group from 0.6 to 1.6 years old by 10.2 and 22.2 %, respectively. Dogs of the second group, aged 1.6–12 years, suffering from urolithiasis (66.6 %), suffer from bacterial diseases 4 times more often compared to animals aged 0.6–1.6 years. Streptococcus urinae, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus were isolated and identified from the urine of dogs in this group. In the associated form, E. coli and Streptococcus pyogene predominated in the urine of dogs. A resistant strain of Klebsiella pneumonia to amoxicillin, streptomycin, kanamycin, gentamicin and tetracycline was identified. Antibiotics should be used rationally, taking into account that for the treatment of animals it is necessary to determine the sensitivity of the pathogen with which the animal was infected. Antibiotic therapy should be carried out until complete elimination, which is sometimes achieved through prolonged treatment. Key words: microorganisms, distribution, pathogens, urine, dogs, resistance, antibiotics, urinary tract, bacteriological testing.

АНАЛІЗ ПОКАЗНИКІВ КРОВІ ТА КОПРОГРАМИ ПРИ ІНФЕКЦІЙНИХ ПАТОЛОГІЯХ ТРАВНОГО ТРАКТУ У СОБАК

Реакцію організму в разі будь-якого захворювання оцінюють не тільки за клінічними ознаками, але й за результатами досліджень біологічних субстратів організму. У процесі вивчення основних гематологічних показників собак, клінічно хворих на інфекційні патології травного тракту, виявлено помірну еритроцитопенію, стійку лейкоцитопенію, олігохромемію, гіпопротеїнемію, зниження кольорового показника крові, гематокритної величини та збільшення ШОЕ. Показники лейкограми свідчать про розвиток помірної нейтрофілії, чітко вираженої лімфоцитопенії, що призводить до виникнення імуносупресії, більш важкому перебігу хвороби. Копрограма собак із гострим перебігом інфекційних ентеропатій характеризувалась підвищеним вмістом білка, стеркобіліногену, білірубіну, неперетравленого крохмалю, мил і нейтрального жиру. Отже, зміни гематологічних показників і копрограми за інфекційних патологій травного тракту собак об’єктивно треба брати до уваги під час вибору терапевтичних засобів із метою ефективного їх лікування.

Relevance of imaging examinations in the diagnosis of mechanical urinary retention in dogs

The pathology of the urinary tract in dogs take on different aspects, urolithiasis and prostate lesions representing the most frequent causes for the presentation to the veterinary specialist. Urolithiasis refers to the development of uroliths along the urinary tract, which can be found in the kidneys, urethra, urinary bladder and/or ureters. The aim of this article is to describe the clinical signs and diagnostic imaging of urolithiasis and prostatic pathology in a group of dogs evaluated in the hospital of the Faculty of Veterinary Medicine, Bucharest. Urolithiasis and the prostatic pathology are the result of complex disorders at the level of the urinary tract, but also at the level of the whole body. In practice, a complete clinical evaluation is necessary in order to apply an appropriate therapeutic approach.

Puerperal Septic Fever and Helminthosis in a 5-Year-Old German Shepherd Bitch

Puerperium is a period post-partum characterized by physiological changes whereby the upper and lower reproductive tracts of the female genital system progressively tries to histologically and endocrinologically return to its pre-gravid state and the period is accompanied by adequate uterine tone for lochia elimination and epithelial regeneration (Voorwald and Tiosso, 2015). Normally, during puerperium the bitch exhibit initially greenish discharge (due to marginal haematoma), then serosanguinous vaginal discharge for 1–6 weeks being the time considered, as the time of normal uterine involution (Feldman and Nelson, 1986). In the first three weeks brownish, non-odorous lochia will be discharged from the vulva, gradually decreasing in volume after the first week (Von Heimendahl et al., 2009). In bitches, the normal uterine involution requires more and adequate time compared to other species due to its nature of the endothelial placentation and deep uterine wounds created at placentation sites after detachment (Al-Bassam et al., 1981). The duration of the puerperium/periparturient period in the bitch has been defined to be 12 weeks (Al-Bassam et al., 1981, Orfanou et al., 2009) or 15 weeks (Yeager et al., 1990). The Bitch, being a monoestrous, polytococcus, non-seasonal animal has a physiological anestrous stage that lasts between 15 to 30 weeks following either parturition (whelping) or diestrous which is characterized by normal quiescence of the gonads and the tubular reproductive tracts. The peripaturient period that occurs during the anaestrous stage of the reproductive/oestrous cycle of the bitch is very delicate and animals are usually susceptible to varying pathological conditions owing to stress and increased demands from the environment and the newborns. For this reason, follow-up of uterine involution is essential to ensure future reproductive capacity/fertility, since failure to complete reproductive organs regression can lead to endometritis, metritis and placental retention (Hirt et al., 2000; Magata et al., 2013). Many assumptions were raised due to lots of biological activities such as nursing of the puppies, uterine involution, milk production and hormonal changes that take place during postpartum period. Thus, bitches are supposed to be more susceptible to pathologic conditions and therefore, it is important to monitor vital functions, appetite and behaviour of postpartum bitches on a daily basis to enable early detection of illness (Grundy and Davidson, 2004). Increase demand in lactation/milk production may predispose the bitch to hypocalcaemia (puerperal tetany) during this period and also a few days post whelping the cervix is physiologically open, paving ways for ascending uterine infection by pyogenic microbes which may lead to septicemia. Thus, a good health surveillance by both the owners and veterinarians is of paramount significance.Infestation of the gastro-intestinal tract and other organs of the animal body by both the adult and immature stages of endo-parasitic helminths is well documented in dogs, especially in newly whelped or neonates and it constitute one of the main causes of pathologies of the intestinal tract in dogs (Blagburn et al., 1996). The life cycle of the many occurring canine helminth species usually involved a vertical transmission from the dam to her offspring. The route of vertical transmission can be: prenatal (e.g Toxocara canis), lactogenic (e.g Ancylostoma caninum, Strongyloides stercoralis) or via coprophagy (Echinococcus multilocularis, Filaroides hirthi, Oslems osleri). Hypobiosis or developmental arrest allows for certain and specific life-cycle stages of the helminthic parasites (mostly L3) to be temporary in-activated in the somatic tissues of the host until the condition for survival is conducive. Such occurs mostly during pregnancy or when the immunity of the host is severely compromised (Shoop, 1999). The L3 re-activates during gestation and is transmitted to the offspring or neonates via milk during puerperal period, the parasites then infest the intestinal tract of the neonates and cause pathologies such as protein loss, diarrhea, obstruction of lumen, iron-deficiency anaemia (especially hook worms) and mortality in unattended condition (Cromptom, 1989).

Screening of Faecal Samples of Pet Dogs for the Presence of Canine Parvoviral Infection by using Haemagglutination Assay and Polymerase Chain Reaction Test

Background: Canine parvovirus-2 causes inflammation of the gastrointestinal tract in dogs. Dogs from Mhow and Indore area suffering from symptoms related to gastroenteritis were investigated for the presence of Canine parvovirus-2 infection. Methods: A total of 50 faecal samples from dogs (33 male and 17 female) were collected individually in 5 ml of phosphate buffer saline solution in sterile containers. These samples were tested in haemagglutination assay using pig red blood cells for the presence of Canine parvovirus-2 specific antigen and in a molecular test, polymerase chain reaction, using primers to amplify Canine parvovirus-2 specific product of 681 base pairs. Result: A total of 5 faecal samples (10%) tested positive in haemagglutination assay indicative of presence of Canine parvoviral-2 antigen in the faecal material. Haemagglutinating titre for positive samples ranged from 32 to 1024. Only one faecal sample found positive in polymerase chain reaction test for amplification of Canine parvovirus-2 specific product. The results of the present study indicate presence of Canine parvoviral-2 infection in pet dogs suffering from gastroenteritis, however at a low level.

Case study on the clinical and anatomopathological descriptions of dog with primary uveal lymphoma

Intraocular lymphoma is a malignant neoplasm, which commonly affects the uveal tract of dogs, most of the time, a consequence of the multicentric by the hematogenous spread of neoplastic lymphocytes. The symptoms are varied and the chronicity can cause serious eye problems such as glaucoma and loss of visual acuity. In view of the unusual occurrence of primary ocular lymphoma in dogs, the objective was to discuss the case of a dog with uveal involvement, presenting conjunctival congestion, increased episcleral vessels, hypopyon, hyphema and increased intraocular pressure. After seven days of conservative treatment, symptoms worsened, especially the intraocular pressure. The tutor returned only two months after the consultation with the sonographic report of neoplasia in the uvea. There was an increase in the ocular bulb, vascularization and corneal edema, posterior synechia, discomfort, loss of visual acuity and neoformation in the iris. Was opted for enucleation and the histopathology suggested large cell lymphoma in the uveal tract. Two years after surgery, no metastases were observed. It is concluded that lymphoma can affect the uveal tract primarily in dogs, demonstrating different clinical signs and, in addition, early diagnosis and correctly instituted treatment favor quality of life and survival of the affected.

Relationships between congenital peritoneopericardial diaphragmatic hernia or congenital central diaphragmatic hernia and ductal plate malformations in dogs and cats.

To characterize the association between peritoneopericardial diaphragmatic hernia (PPDH) or congenital central diaphragmatic hernia (CCDH) and ductal plate malformations (DPMs) in dogs and cats. 18 dogs and 18 cats with PPDH or CCDH and 19 dogs and 18 cats without PPDH or CCDH. Evaluation of clinical details verified PPDH or CCDH and survival times. Histologic features of nonherniated liver samples were used to categorize DPM. Immunohistochemical staining for cytokeratin-19 distinguished bile duct profiles per portal tract and for Ki-67-assessed cholangiocyte proliferation. Histologic features of herniated liver samples from PPDH or CCDH were compared with those of pathological controls (traumatic diaphragmatic hernia, n = 6; liver lobe torsion, 6; ischemic hepatopathy, 2). DPM occurred in 13 of 18 dogs with the proliferative-like phenotype predominating and in 15 of 18 cats with evenly distributed proliferative-like and Caroli phenotypes. Congenital hepatic fibrosis DPM was noted in 3 dogs and 2 cats and renal DPM in 3 dogs and 3 cats. No signalment, clinical signs, or clinicopathologic features discriminated DPM. Kaplan Meier survival curves were similar in dogs and cats. Bile duct profiles per portal tract in dogs (median, 5.0; range, 1.4 to 100.8) and cats (6.6; 1.9 to 11.0) with congenital diaphragmatic hernias significantly exceeded those in healthy dogs (1.4; 1.2 to 1.6) and cats (2.3; 1.7 to 2.6). Animals with DPM lacked active cholangiocyte proliferation. Histologic features characterizing malformative bile duct profiles yet without biliary proliferation were preserved in herniated liver lobes in animals with DPM. DPM was strongly associated with PPDH and CCDH. Because DPM can impact health, awareness of its coexistence with PPDH or CCDH should prompt biopsy of nonherniated liver tissue during surgical correction of PPDH and CCDH.

Efficacy of a phospholipid-stabilized sulfur hexafluoride microsphere contrast agent and water for hydrosonography of the upper portion of the gastrointestinal tract in dogs

To investigate the efficacy of a phospholipid-stabilized sulfur hexafluoride microsphere (SHM) contrast agent and water for hydrosonography of the upper portion of the gastrointestinal tract of dogs. 12 healthy adult Beagles. In a crossover study, each dog was anesthetized and underwent noncontrast ultrasonography then hydrosonography following administration of tap water (30 mL/kg) without (water method) or with SHM (0.1 mL; SHM method) via an orogastric tube. There were at least 3 days between hydrosonographic procedures. Wall thickness, wall layer definition, conspicuity of the mucosal-luminal interface, and image quality were evaluated separately in the near and far fields for the gastric cardia, body, and pylorus and descending duodenum and compared among the 3 scanning methods. Mean wall thickness measurements did not differ significantly between the water and SHM methods at any location except the far-field gastric cardia where the mean wall thickness for the SHM method was less than that for the water method. In general, the SHM method improved wall layer definition and conspicuity of the mucosal-luminal interface of structures in the near field, compared with noncontrast method. The water and SHM methods both resulted in superior image quality relative to the noncontrast method for the near-field gastric cardia, far-field gastric cardia, and far-field duodenum. Results indicated that, for dogs, gastrointestinal hydrosonography by use of the SHM method improved wall layer definition and mucosal conspicuity, particularly in near-field images of the upper portion of the gastrointestinal tract.

Isolation of Staphylococcus spp. and Pseudomonas spp from small animals' clinics: A potential hazard for nosocomial infections

Veterinary clinic environments contain a variety of pathogens that can cause infection in animals. Pseudomonas aeruginosa is one of the most common Gram-negative nosocomial pathogens due to its versatility in nutrients and universal presence. Staphylococcus pseudintermedius and Staphylococcus aureus usually colonize the skin, mucous membranes, urogenital tract, and occasionally the alimentary tract in dogs and humans. This study was carried out on 250 samples collected from different clinics in Egypt for isolation of Staphylococcus and Pseudomonas spp. Among 250 samples, 125 samples were used for Staphylococcus isolation, revealing 77 samples had staphylococcus, with high count in floor and examination table respectively, while another 125 samples were used for Pseudomonas isolation, revealing 80 samples showed growth of Pseudomonas with high count in floor and examination table respectively. present especially in the examination table and floor. The improper cleaning program and disinfection lead to high results for isolated Staphylococcus and Pseudomonas species in the environmental surfaces in small animal's clinics

Impact of specimen type on findings for bacterial composition within the intestinal tract of dogs and cats with and without chronic enteropathy.

To compare bacterial diversity and community composition among fecal, rectal swab, and colonic mucosal biopsy specimens from dogs and cats with and without chronic enteropathy (CE). 9 healthy dogs, 8 dogs with CE, 8 healthy cats, and 9 cats with CE. In a cross-sectional study design, fecal, rectal swab, and colonic mucosal biopsy specimens were obtained by colonoscopy from healthy dogs and dogs and cats with CE. Fecal and rectal swab specimens were collected from healthy cats. Genomic DNA was extracted, the 16S rRNA V4 gene region was amplified, and sequencing was performed by use of primers 515F to 806R on a paired-end platform. For healthy dogs and dogs and cats with CE, bacterial diversity based on the Chao1 estimate of total species richness was higher for colonic mucosal biopsy specimens than for fecal specimens. Analysis of similarities by use of the Bray-Curtis dissimilarity index revealed that the bacterial communities captured in rectal swab specimens were similar to those captured in fecal specimens for healthy dogs and dogs with CE and similar to those captured in colonic mucosal biopsy specimens for both dog groups and cats with CE. Rectal swab and colonic biopsy specimens were successfully used to characterize the bacteriome of the intestinal tract in dogs and cats by 16S rRNA gene sequencing. Although the specimen types evaluated in this study were not interchangeable in results, rectal swab specimens were practical to collect from dogs and cats to study bacterial composition within the intestinal tract and may provide an alternative to colonic mucosal biopsy and fecal specimens.

Carriage of ESBL and Amp-C -b -lactamase among Escherichia coli strains isolated from dogs in kennels Dak Lak province

The global prevalence of antimicrobial resistance and Extended-Spectrum and AmpC Beta- Lactamases is continuously widespread among Escherichia coli during recent years, especially in Viet Nam. In Viet Nam, there have been researches on ESBL and AmpC-carrying E. coli inhabiting animal and human. However, studies of antimicrobial resistance in E. coli residing in pets, especially dogs are unavailable. The aim of the study was to investigate the antimicrobial sensitivity testing (AST), the resistance to 3rd cephalosporin and penicillin, also to assess the molecular detection of ESBL and Amp-C-beta -lactamase in E. coli isolates inhabiting the digestive tract of dogs at kennels Dak Lak. By using double disk synergy test (DDST), and ceftazidime-imipenem antagonism test (CIAT) to detect phenotypic characteristic of E. coli strains producing extended-spectrum beta- lactamases (ESBLs) and plasmid-mediated Amp-C-beta -lactamase, and by using multiplex polymerase chain reaction (multiplex PCR) to confirm the presence of ESBL genes (class A): blaCTX-M(1;2;8;9;25), bla TEM, bla SHV , bla OXA and genes encoding AmpC-type beta lactamase (class C): bla MOX-1;2 , bla CMY- (1;2-7;8-11) , blaLAT-(1;4) ,bla DHA-(1;2), bla ACC, bla FOX-(1-5B) ,bla MIR-1 ,bla ACT-1. From of three hundred twelve bacteial strains isolated from sixty-four rectal swabs two hundred sixty-nine E. Coli, isolates accounting for 86%, were identified and isolated, forty-four (16%) and twelve (4%) E. coli isolates encoding with ESBL and Amp-C-beta -lactamases. From molecular diagnosis with regard to phenotype, production of ESBL was shown in thirty-nine (15%) E. coli isolates and Amp-C enzymes in eight (3%) E. coli isolates. The high percentage of E. coli exhibiting antibiotic resistance revealed the accelerated overuse of antibiotics. Result of this study will contribute to the monitoring of epidemiologic resistance.

Dysregulation of gastrointestinal RAGE (receptor for advanced glycation end products) expression in dogs with chronic inflammatory enteropathy

The pathogenesis of chronic inflammatory enteropathies (CIE) in dogs involves dysregulated innate immune responses. The receptor for advanced glycation end products (RAGE), a pattern recognition receptor, plays a role in chronic inflammation. Abrogation of proinflammatory RAGE signaling by ligand binding (e.g., S100/calgranulins) to soluble RAGE (sRAGE) might also be a novel therapeutic avenue. Serum sRAGE levels are decreased in canine CIE, but gastrointestinal tissue RAGE expression has not been investigated in dogs. Thus, the study aimed to evaluate the gastrointestinal mucosal RAGE expression in dogs with CIE. Further, the potential binding of RAGE to canine S100/calgranulin ligands was investigated.Epithelial RAGE expression was quantified in gastrointestinal (gastric, duodenal, ileal, and colonic) biopsies from 12 dogs with CIE and 9 healthy control dogs using confocal laser scanning microscopy. RAGE expression was compared between both groups of dogs and was tested for an association with patient characteristics, clinical variables, histologic lesion severity, and biomarkers of extra-gastrointestinal disease, systemic or gastrointestinal inflammation, function, or protein loss. Statistical significance was set at p < 0.05. RAGE:S100/calgranulin binding was assessed by immunoassay and electrophoretic techniques.RAGE expression was detected in all 59 biopsies from diseased and healthy control dogs evaluated. Epithelial RAGE expression in the duodenum and colon was significantly higher in dogs with CIE than in healthy controls (p < 0.04). Compared to healthy controls, RAGE expression in dogs with CIE also tended to be higher in the ileum but lower in the stomach. A slight (statistically not significant) shift towards more basal intestinal epithelial RAGE expression was detected in CIE dogs. Serum sRAGE was proportional to epithelial RAGE expression in the duodenum (p < 0.04), and RAGE expression in the colon inversely correlated with biomarkers of protein loss in serum (both p < 0.04). Several histologic morphologic and inflammatory lesion criteria and markers of inflammation (serum C-reactive protein and fecal calprotectin concentration) were related to epithelial RAGE expression in the duodenum, ileum, and/or colon. in vitro canine RAGE:S100A12 binding appeared more pronounced than RAGE:S100A8/A9 binding.This study showed a dysregulation of epithelial RAGE expression along the gastrointestinal tract in dogs with CIE. Compensatory regulations in the sRAGE/RAGE axis are an alternative explanation for these findings. The results suggest that RAGE signaling plays a role in dogs with CIE, but higher anti-inflammatory decoy receptor sRAGE levels paralleled RAGE overexpression. Canine S100/calgranulins were demonstrated to be ligands for RAGE.

Study on various affections of lower urinary tract in dogs

A total of 55 cases were selected with the different affections of lower urinary tract from year September-2017 to July-2019. The age and sex wise distribution of different disorders of lower urinary tract revealed the age of dogs ranged from one and half year to eighteen years. The age and sex wise distribution of different disorders of lower urinary tract revealed the age of dogs ranged from one and half year to eighteen years. Urolithiasis (52.73 %, n=29) was higher in 7 to 9 years aged dogs (18.18 %), in male dogs (38.18 Vs. 14.55 %). The dogs having non-urolithiasis conditions (n=26, 47.27 %), comprised percent cystitis (n=17), mass in bladder (n=8) (30.91 and 15.54, respectively) and a case of lodged catheter in bladder. The incidence of cystitis and mass in bladder were higher in the age of up to 3 (14.54 %) and 7 to 9 years (9.10 %), respectively. The male dogs were found to be suffering more from cystitis (25.45 %) and mass in bladder (36.36 %) than female dogs (5.45 % and 9.09%, each affection). Labrador and Pug dogs had higher urolithiasis and cystitis up to 14.54 and 7.23 %, each, respectively, whereas German Shepherd had a mass in bladder up to 5.45 %. The dogs given commercial food had higher (31.03 %) urolithiasis. The morbidity period of 24 to 48 hours was observed in dogs having urolithiasis (41.38 %) and cystitis (52.94 %) whereas for dogs having mass in the bladder (52.50 %) had the morbidity lasting for more than a week. The most pronounced clinical signs in dogs having urolithiasis and cystitis were distended bladder in 48.28 % (14) and 70.59 % (12), respectively, whereas haematuria in all cases of mass in bladder. A dog with a lodged catheter in bladder was morbid for more than 72 hrs with a dribbling of urine.

Retrospective Analysis of Lower Urinary Tract Disorders in Dogs: Study of Five Years

Background: The data pertaining to the lower urinary tract disorders in dogs in India is very merge. This retrospective analysis presents a retrospective analysis of incidence of affections of lower urinary tract in dogs. Methods: The cases of different affections of lower urinary tract presented at the Veterinary Clinical Complex, Veterinary College, Anand Agricultural University, Anand during April-2012 to March-2017 were screened and detailed information regarding age, sex and breed were collected and analysed to calculate percentage of incidence of different affections of lower urinary tract viz., surgical affections and non-surgical affection. Conclusions: Total 24319 cases of lower urinary tract affections in dogs were registered. Among them 5854 (24.07 %) surgical cases comprising urolithiasis (81, 76.42 %), bladder neoplasia (25, 23.58 %) and 18465 (75.93 %) non-surgical affections with cystitis (176, 0.95 %) cases. The highest incidence of urolithiasis was 25.93 per cent in the year 2014-15 and in 7 to 9 years aged dogs (35.80 %). The sex wise per cent incidence was higher in male (72.84) than female (27.16). The urinary bladder calculi was the highest (72.84 %) followed by the urethra (16.05 %) and both the locations (11.11 %). The year wise highest per cent incidence of cystitis was recorded for the year 2015-16 (52.27). The age wise per cent incidence of cystitis was equally higher 28.98 in the age groups of 7 to 9 and 10 to 12 years, each. The year wise the highest per cent incidence of neoplasia of urinary bladder (TCC) in dogs was 52.00 for the year 2015-2016. The age wise percent incidence of neoplasia of urinary bladder was the highest in 7 to 9 years age groups (36.00). The sex wise per cent incidence for neoplasia in urinary bladder was higher in male (64.00) than female (36.00). The breed wise highest per cent incidence of urolithiasis was in Pomeranian (25.93), cystitis in Pug (28.41) and neoplasia of bladder (TCC) Labrador and Pug (20.00, each).

ПОШИРЕНІ ПАТОЛОГІЇ У СОБАК ТА ГЕПАТОПРОТЕКТИВНІ ЗАСОБИ

Досліджено поширення патологій гепатобіліарної системи у собак і спектр гепатозахисних препаратів. Визначено, що основна частка гепатопатій серед собак має вторинний характер – 81,8 %. Патології печінки виникають, переважно, як наслідок хронічної серцевої недостатності і токсичного навантаження організму тварин. Як самостійне захворювання, гепатопатії зареєстровано у 18,2 % собак. Встановлено, що структура гепатопротекторів для тварин сформована, в більший мірі, комбінованими препаратами – 71,4 %.

An epizootic situation is in relation to the nematodosiss of gastroenteric channel of dogs

Gastrointestinal nematodes are prevalent among dogs in Ukraine and most other countries. Economic losses in dog breeding for invasive diseases are diffi cult to calculate in material terms, but they are clearly manifested in terms of animal health. Therefore, the purpose of the research was to fi nd out the epizootic situation regarding the nematodes of the gastrointestinal tract in dogs of diff erent age, sex and breed groups in the Belotserkovsky district of Kyiv region. The studies were conducted on the territory of single-sector private sector farms of 15 settlements of Belotserkovsky district and on the territories of shelters for homeless animals of Belaya Tserkov. Koprovoskopichesky researches were carried out by the combined method standardized by Kotelnikov - Hrenov. According to the results of coprovoscopy of samples from dogs of the Belotserkovsky area, a signifi cant spread of nematodes of the gastrointestinal canal was established (EI was 50.18%). Eggs of such nematodes as Trichuris vulpis, Toxocara canis, Ancylostoma caninum, Toxascaris leonina, Strongyloides stercoralis were isolated. Diseases were recorded in the form of mono-invasions, among which the largest proportion of the aff ected were trihuriza (27.11%), toxocariasis (6.59), ankylostomosis (1.83%), and myxinvasions, which again led the trichuris. Animals from birth to 7 years were most aff ected. In terms of gender, females were more vulnerable. The highest incidence of invasion was among outbred dogs (51.56%). Most dogs in this group are aff ected by trichuris (54.55%) and myxinvasia (29.29%), which also included T. vulpis. Breeds German Shepherd, Dachshund, Russian Spaniel had the highest percentage of nematode infestations among all studied breeds. The leader among pathogens was T. vulpis, which covered more than half (52.63%) of rocks, myxinvasions were 28.94%, which included T. vulpis. The breeds of the Belgian shepherd, shih-tzu, Russian-European chamomile, alabai were resistant. Key words: dog, invasion, trichrosis, toxocariasis, ankylostomosis, toxascarose, distribution, koproovoskopichni research, extensiveness of invasion, intensity of invasion.

Endoscopic Removal of Foreign Body in Upper Gastrointestinal Tract in Dogs: Success Rate and Complications

Background: Dogs and cats with acute signs of choking, retching, cough, vomiting, regurgitation, hypersalivation, dysphagia and odynophagia should have the presence of a gastrointestinal foreign body (FB) as part of their differential diagnosis, where it is a frequent condition in the care of small animals. Most objects lodged in the esophagus, stomach, and proximal duodenum can be removed by upper digestive endoscopy, a curative, little invasive procedure. The objective of our study was to evaluate the physical aspects and location of esophageal and gastric FBs observed in 88 dogs and the age and breed of the affected animals and to determine the success rate and eventual complications associated with the procedure as well.Materials, Methods &amp; Results: Eighty-eight cases of dogs, males and females of varying ages and breeds, submitted to upper digestive endoscopy were selected because of suspicion of esophageal or gastric FBs. The endoscopic procedure aimed at confirming the diagnosis, whether or not followed by endoscopic removal of these objects. Prior to endoscopy, the animals had laboratory tests (blood count and serum biochemistry) and subsequently to the anesthetic protocols of choice for each case. Data including breed, age, type of constituent material and anatomical location of the FB, endoscopic procedure success rate and complications were recorded and descriptively evaluated. Of the 88 dogs evaluated, 60% (n = 53) were male and 40% (n = 35) female. According to the breed of the animals, 55% (n = 49) were small-breed dogs, 29% (n = 25) large-breed dogs, and 8% (n = 7) medium-breed dogs, and 8% were of mixed breed dogs, which could assume various sizes. Shih Tzus accounted for 18% (n = 16) of the animals, Lhasa apsos 8% (n = 7) and mixed breed 8% (n = 7), where these were the most frequently affected breeds. Regarding age, animals 1 to 5 years old represented 66% (n = 58) of the patients, and those 6 to 10 years old accounted for 20% (n = 18), while 11% of the dogs were over 10 years old (n = 10). Two animals (3%) had no information about their ages. Pieces of cloth were the most frequently found FBs, representing 20%(n = 20), followed by animal bones (19%) and fruit pits (10%). As for location, 78% (n = 69) of the FBs were located in the stomach and 22% (n = 19) in the esophagus. The success rate of endoscopic FB removal in this study was 83% (n = 73). In 76% (n = 67) of the animals, there were no complications due to the presence of FB in the upper gastrointestinal tract. The most frequent complications were esophageal ulcerations (n = 7) and the inability to move the FB (n = 5) and adherences. (n = 4).Discussion: The results showed that small-breed dogs, especially Shih Tzus and Yorkshires, represented a larger number of cases, probably due to their popularity in Brazil, where the study was conducted. Males were more prevalent than females, and the most affected age was between 1 and 5 years, with emphasis on younger animals. There were more gastric FB cases compared to esophageal FB cases, which was related to the interval between the ingestion of the object and veterinary care. Although not the most prevalent FB, the high rate of mango pits can be explained by the vast number of mango trees in the Federal District. There were few complications compared to the success of cases, indicating that endoscopy is the procedure of choice for the diagnosis and removal of FBs from the gastrointestinal tract.

Melamine, Asam Suanurat dan Melamin-Sianurat: Kaitan dengan Penyakit Saluran Perkencingan Hewan

Numerous cases of acute renal failure in dogs and cats from 2004-2007 were associated with the ingestion of dog and cat pet food products and the suspected contaminant was identified as melamine and its derivatives such as cyanuric acid and melamine cyanurate. Melamine is a chemical used primarily for the manufacture of plastics, coatings, commercial filters, adhesives and compounds in the plate-making industry and kitchen appliance.But then these compounds have been abused to increase the content of nitrogen compounds in human food products and animal feed products, respectively. Increasing the content of nitrogen compounds from melamine aims to increase the value of false protein food and feed. Melamine basically has a wide security limit so it is relatively non-toxic if consumed by humans and animals. However, many studies and case reports show that melamine can cause damage to the kidneys especially if present together with cyanuric acid in the body.This paper aims to provide a simple overview and discussion in cases of damage to the urinary tract in dogs and cats that are likely caused by toxicity of melamine and its derivatives are likely to have occurred in Indonesia.