The essential objectives of the present research was to investigate the association between the professional maturity and hope levels with test anxiety of twelfth-grade students in high schools. A total of 700 twelfth class high school students who attend eight different types of high school in Trabzon province throughout the academic year (2016-2017) was recruited for this study. Relational and causal study methods were utilised in the study. Regarding data collection, the Professional Maturity Scale (PMS), State Hope Scale (SHS) and Test Anxiety Inventory (TAI) were selected. In order to analyze the gathered data, the SPSS 23.0 program was used. The relationships between the variables were evaluated by Pearson Moments Multiplication correlation coefficient and the predictive level of the variables was examined by hierarchical regression analysis. The results reveal that the students' test anxiety levels are related to professional maturity and hope, and these variables are important factors explaining the students' test anxiety.