Total Urinary Hydroxyproline Research Articles

Value of urine and serum hydroxyproline in the diagnosis of thyroid disease.

The excretion of free and total hydroxyproline in the urine and the concentration of free hydroxyproline in the serum were determined in 32 patients with hyperthyroidism, in 10 patients with hypothyroidism and in 32 control subjects. All 3 hydroxyproline values were increased in hyperthyroidism and decreased in hypothyroidism, but the values for urinary free hydroxyproline showed a considerably greater overlap with the controls than the other 2 values. The excretion of total hydroxyproline was within the range of the values of the control subjects in none of the patients with hypothyroidism and in only 2 very mild cases with hyperthyroidism, in both of whom this value was right at the upper limit of the range of the control values. A statistically highly significant correlation was found between the values for urinary total hydroxyproline and serum protein-bound iodine. Therapy was accompanied by a rapid normalization of the hydroxyproline values. It is suggested that the determination of urinary total hy...

Total urinary hydroxyproline excretion in diabetics before and after hypophysectomy and after growth hormone in adult hypopituitarism

Total urinary excretion of hydroxyproline has been found to be significantly decreased in adult diabetics and was increased after hypophysectomy for diabetic retinopathy. There was no change in hydroxyproline excretion after the administration of 53 mg. of human growth hormone to one patient with hypopituitarism over a 4 day period.

Urinary hydroxyproline during late pregnancy and postpartum involution

Total urinary hydroxyproline was studied sequentially in each of 4 subjects during late pregnancy and postpartum involution. Three of the 4 subjects showed progressively increasing levels of urinary hydroxyproline during the last trimester. A maximal level was reached immediately postpartum and then fell rapidly during the early postpartum period. The fourth subject showed no change in urinary hydroxyproline during the study. The data indicate that most of the uterine collagen is not proteolytically destroyed during postpartum involution and suggests that a “physiological” osteomalacia exists during late pregnancy.

Correlation of urinary hydroxyproline, serum alkaline phosphatase and skeletal calcium turnover

Thirty-three patients underwent 79 simultaneous calcium 47 kinetic and calcium, phosphorus and nitrogen metabolic balance studies. Concomitantly, total urinary hydroxyproline and serum alkaline phosphatase were determined. The group was weighted in favor of patients with osteoporosis, vitamin D-resistant rickets, metastatic bone disease and parathyroid disorders. Total urinary hydroxyproline, serum alkaline phosphatase, calcium accretion rate and calcium resorption rate correlate well among the metabolic bone disease groups. There is a good correlation in a given individual between serum alkaline phosphatase and calcium accretion rate and between urinary hydroxyproline and calcium resorption rate. The combination of serum alkaline phosphatase and urinary hydroxyproline is a useful screening method for the detection of skeletal diseases with high turnover rates.

URINARY HYDROXYPROLINE EXCRETION IN NORMAL CHILDREN AND ADOLESCENTS.

SummaryTwenty-four hour urinary total hydroxyproline was measured in 70 normal males and 10 normal females between the ages of 5 through 49. Normal values for excretion of hydroxyproline were found...

An oral gelatin-xylose test for estimating pancreatic proteolytic activity

An oral gelatin-xylose test is proposed as a practical test for pancreatic proteolytic activity. The principal function of the xylose test is to support the adequacy of small-bowel absorption and thus allow for the more confident interpretation of protein digestion. It was found that, following gelatin ingestion, the normal adult increases his urinary total hydroxyproline excretion by a mean of 7.7 mg./hr. Values falling below 3.3 mg./hr. after a test meal are considered abnormal. A practical short testing period in children is feasible because of the rapid renal clearance of the absorbed hydroxyproline peptides. These peptides are the principal form of urinary hydroxyproline.