Total Unsaturated Fatty Acids Research Articles

Performance, gut morphology, and meat characteristics of broilers housed at a high-density pen and provided with fermented Averrhoa bilimbi fruit filtrate.

This study assessed the impact of fermented Averrhoa bilimbi fruit filtrate (FF) on growth, gut morphology, and meat traits of high-stocked broilers. A 2 × 2 factorial trial with stocking densities (9 or 18 birds/m2) and drinking 2% FF or plain water was conducted using 378 14-day-old broiler chicks. On day 35, samples were obtained and analyzed. FF improved feed efficiency and income over the feed cost of high-stocked broilers by about 7.63% and 10%, respectively, compared to high-stocked broilers receiving only water. FF decreased duodenal crypt depth and meat water-holding capacity. Meats from high-stocked broilers receiving FF showed lower cholesterol than other meats. Lower cholesterol/high-density lipoprotein (HDL) and higher HDL/low-density lipoprotein were found in meats from broilers receiving FF. Total unsaturated fatty acid (UFA) was higher in meats of high-stocked broilers receiving FF than others. The UFA/saturated fatty acid was lower in meats of high-stocked broilers receiving plain water. High-stocked broilers given FF had higher meat n-3 polyunsaturated fatty acid (PUFA). FF enhanced meat n-6 PUFA levels. The n-3/n-6 PUFA increased with high density and drinking FF. Drinking FF improved gut morphology and meat qualities of broilers housed in high-density pens. FF may be an excellent alternative to improve the growth and meat qualities of broilers raised in high-density houses.

1H MRS Analysis Using Ultrashort-TE Sequence of Hepatic Lipids in a 9.4T MRI Device

Non-alcoholic fatty liver disease is the most common cause of chronic liver diseases which is characterized by increased levels of obesity, and diabetes. Liver lipid content has been suggested to play an important pathogenic role in the development of liver fibrosis, and cirrhosis In particular, it is associated with the risk of hepatocellular carcinoma (HCC). Liver biopsy is still the gold standard for diagnosing and assessing the disease. However, the invasive and limited tissue sampling of the biopsy presents problems. Proton magnetic resonance spectroscopy (1H-MRS) enables the study of cellular biochemistry and metabolism and provides a non-invasive means to determine disease abnormalities and progression in vivo and longitudinally. With the increased availability of high-field magnetic resonance (MR) systems for clinical and preclinical studies, both signal-to-noise ratio (SNR) and spectral resolution of metabolites in the MR spectra can be improved significantly, allowing more accurate metabolite identification, quantification, and thus disease characterization. 1H-MRS permits longitudinal assessment of fat fraction, saturated and unsaturated. This study aims to characterize early hepatic lipid changes in the fatty liver mouse model by in vivo short-echo time (TE) 1H-MRS (Proton- Magnetic Resonance Spectroscopy). This study examined 17 male C57BL/6 mice, including 8 high-fat diet (45%) mice for 20 weeks and 9 normal mice for 17 weeks. C57BL/6 mice were fed with 60% high-fat diet containing 60% fat, 20% protein, and 20% carbohydrate. MR imaging with single-voxel 1H-MRS was performed using a PRESS sequence at 9.4T magnetic resonance imaging (MRI). The full width at half maximum (FWHM) ranged from 4 to 8 Hz. Water suppression was accomplished with "VAPOR" pulses. The examination (voxel size, 2×2×2 mm3) was performed from the liver parenchyma in mice at normal and high-fat diets, respectively. The spectral acquisition parameters were TR/TE = 2500/16 ms, and 256 acquisitions for averaging. LCModel fitting was conducted using an experimental basis sets. Less than 10% standard deviation (%SD) of metabolite quantification data was allowed. The areas under the peaks were measured as follows: signal integrals of lipid methyl protons (-CH3; 0.9 ppm), methylene proton ((-CH2-)n; 1.3 ppm), allylic protons (- CH2-C=C-CH2; 2.1 ppm), α-methylene protons (-CO-CH2- CH 2; 2.3 ppm), diallyl ic protons (=CCH-2C=; 2.8 ppm), glycerol protons (CH2-COO; 4.1 ppm), glycerol protons (CH2-COO; 4.3 ppm) and methene protons (CH=CH; 5.3 ppm). For relative quantification, total lipid (TL), total saturated fatty acid (TSFA), total unsaturated fatty acid (TUFA), total unsaturated bond (TUB), polyunsaturated bond (PB) and choline-containing compound (Cho) were normalized by separating each peak of (-CH2-)n, -CH2- C=C-CH2-, -CO-CH2-CH2=C-CH-2-C= and –CH=CH-.Significant increases in lipid signals of 0.9, 1.3, 2.1, 2.3, 2.8, 4.1, 4.3, and 5.3 ppm were found in animals at high-fat diet(p<0.01, p<0.001). TL, TSFA, TUFA, TUB, PUB and Cho was significantly increased at high-fat diet(p<0.01, p<0.05). Therefore, 1H-MRS is useful in detecting and characterizing various hepatic lipid alterations.

Mixing Ratio Optimization of Silkworm, Mealworm, and White Grub Using Response Surface Methodology

Purpose: In this study, the optimal mixing ratio of total branched-chain amino acid (BCAA) content, total unsaturated fatty acid content, antioxidant activities, and enzyme activities of silkworm, mealworm, and white grub, which are alternative protein sources, was derived using response surface analysis.Method: Silkworm, mealworm, and white grub were the independent variables, and total BCAA content, total unsaturated fatty acid content, total polyphenol content, total flavonoid content, ABTS radical scavenging activity, α-glucosidase inhibitory activity, and ACE inhibitory activity were used as the dependent variables to determine the optimal mixing ratio.Result: The optimal mixing ratio for total BCAA content, total unsaturated fatty acid content, antioxidant activities, and enzyme activities derived from the response surface analysis was silkworm (X<sub>1</sub>) 2.998 : mealworm (X<sub>2</sub>) 0.623 : white grub (X<sub>3</sub>) 1.983. At this ratio, the total BCAA content was 0.52 g, the total unsaturated fatty acid content was 0.44 g, the total polyphenol content was 67.02 mg TAE/g, the total flavonoid content was 35.58 mg QE/g, the ABTS radical scavenging activity was 95.61%, the α-glucosidase inhibitory activity was 37.79%, and the ACE inhibitory activity was 95.25%.Conclusion: It is expected that the optimal mixing ratio of silkworm, mealworms, and white grub derived in this study can be used to develop products for the management of various chronic diseases.

Physico-chemical, proximate, mineral and bioactive composition of Garcinia buchananii baker fruit from Uganda and Rwanda

Garcinia buchananii Baker (Family,Cluciaceae/Guttiferae) is an underutilized indigenous fruit tree that produces edible fruits that are used as both food and medicine in tropical Africa. This study evaluated the physical and chemical characteristics of G. buchananii fruits collected from Uganda and Rwanda. Ripe fruits were harvested during their peak seasons in Masaka and Bugesera Districts in Uganda and Rwanda, respectively. The fruits were analyzed for physical characteristics including; fruit weight, fruit size (that is length and width), number of seeds per fruit, seed weight, seed dimensions(length, width and breadth); chemical characteristics (pH and titratable acidity), proximate composition (moisture content, protein, fat, dietary fibre, carbohydrates and energy), mineral composition (K, Na, Ca, Mg, Ca, Fe, Zn, Cu), and bioactive components (total phenolic compounds, flavonoids and anthocyanins; and antioxidant activity). Data were statistically analyzed using a student T-test (p≤0.05). The results indicated that fruit weight and pulp content ranged from 6.3±1.3-9.5±2.8g and 54.1±10.6- 81.1±6.5%, respectively. Titratable acidity of the pulp ranged from 6.1±0.8 to 7.1±0.1 %. Nutritionally, dietary fibre, vitamin C, iron and zinc ranged from 20.0±0.4 -22.6±1.8 g/100g, 32.8±3.2-42.0±3.3 mg/100g, 4.8±0.2 - 6.5±0.8 mg/100g and 1.1±0.0 -2.5±0.1 mg/100g, respectively. The total phenolic compounds and antioxidant activity ranged from 996.7±50.5 - 1147.5±47.4 mg/g GAE (Garlic Acid Equivalent) and 8.0±0.2- 8.4±0.1 mg/100g AAE (Ascorbic Acid Equivalent) as IC50, respectively. There was a significant difference (p≤0.05) in the physical characteristics (weight, length, width and breadth), nutritional composition and bioactive components of the fruit samples of the two countries. Assessment of the nutrients indicated that the pulp was rich in vitamin C, iron, zinc, copper and dietary fibre. The pulp also contained phenolic compounds with antioxidant activity. The seeds had 6-24% crude oil content with palmitic, stearic, oleic and linoleic as major fatty acids. The total unsaturated fatty acids in the seed oil ranged from 58.4-59.5%. Although this finding showed significant differences (P≤0.05) in the physical and chemical of G. buchananii fruit from Uganda and Rwanda, the nutritional composition and bioactive component information has shown the potential of the fruits for processing into high-value nutraceuticals.

Macronutrient accumulation and cytological and morphological response of safflower affected by the foliar application and harvest date

The present experiment was conducted to investigate the effect of the foliar application of growth stimulators and harvest date on plant growth and quality attributes of safflower at the Agricultural Research station of Urmia (Iran) during the 2015–2016 and 2016–2017 growing seasons.The experiment was carried out in a factorial randomized complete block design with 10 treatments and four replications. The first factor was the foliar application of (i) nano-Fe 2 mg L−1, (ii) humic acid 2.5 mL L−1, (iii) magnetic water at 2 L m−2, (iv) ascorbic acid 30 mg L−1, and (v) control (distilled water), and the second factor was two harvest dates including of 121 and 136 days after sowing. The highest total unsaturated fatty acid content was obtained from the foliar application of nano-Fe. This treatment also resulted in a 12% increase in the total unsaturated fatty acid. The foliar application of humic acid and nano-Fe significantly promoted the growth of the safflowers via improving photosynthetic characteristics, such as chlorophyll index. The highest total N (3.65 ppm), available P (0.362 ppm), and K (1.32 ppm) contents of the seeds were obtained from the second harvest date. As well, the total unsaturated and saturated fatty acid percentage and harvest index were significantly affected by the second harvest date. Percent of chromosome abnormality was also decreased on the second harvest date.

Physiological impact of putrescine on Trigonella foenum-graecum L. growing under temperature stress

Abstract It is important to determine the effect of the rapid environmental changes on plant physiological processes which were reflected on plant production and find the ways to alleviate their effects. This study was performed to study the role of putrescine in ameliorating the harmful effects induced by temperature stress. Priming treatment was applied to Trigonella by soaking the seeds in 5mM putrescine before exposure to the different temperature degrees (15 °C, 25 °C, 35 °C or 40 °C). At the age of 120 days old some yield parameters and physiological processes were measured. The results indicated that, 15 °C, 35 °C or 40 °C significantly decreased the yield parameters, carbohydrates and soluble proteins. Amino acids and phenols are accumulated in seeds which is considered an acclimation mechanism of plant against temperature stress. The results also, indicated an increase in the total unsaturated fatty acids and a decrease in the thickness and intensity of the β-esterase isoforms in plant seeds subjected to 15 °C and 35 °C. Temperature stress affects the plant production and application of putrescine improves the harmful effects.

Carcass Composition and Quality of Meat of Pulawska and Pulawska x PLW Crossbred Pigs Fed Rations with Naked Oats.

Simple SummaryDue to the presence of fibrous hulls, the use of traditional cultivars of oats (Avena sativa L.) in feed rations for pigs is restricted. The development of naked oat cultivars (Avena nuda L.) containing only about 2% of crude fibre allowed this cereal grain to be included in the diets of monogastric animals. The present studies evaluated the efficiency of a 60% inclusion of naked oats in rations fed to Pulawska pigs and Pulawska x Polish Large White (PLW) crossbreds on their performance results (weight gain, feed conversion), carcass composition and physical (pH, colour, WHC) and chemical (essential nutrients, fatty acids) characteristics of meat. It was demonstrated that a 60% inclusion of naked oats is recommended in the diets of Pulawska pigs and Pulawska x PLW crossbreds to maintain carcass composition and meat quality comparable to that of pigs fed with rations containing barley only. The performance indicators of Pulawska pigs declined, but their meat quality traits improved in terms of nutritional value, as the n-6/n-3 acids’ ratio was narrower than that of Pulawska x PLW.The study aimed to determine the impact of a 60% inclusion of naked oats in feed rations for Pulawska pigs and Pulawska x Polish Large White (PLW) crossbreds on the fattening performance, carcass composition, and meat quality. It was demonstrated that—independent from their diet—Pulawska pigs showed a daily weight gain about 14.5% lower (p ≤ 0.05), with about 15% higher feed conversion rate. The experimental factors showed no impact on the dressing percentage, meatiness, and backfat thickness, except that the “eye” of the loin was significantly smaller (by 4.55 cm2) in Pulawska pigs. There was no interaction effect (diet/breed) for the pigs’ performance results and carcass composition. A 60% inclusion of naked oats in the pigs’ diet did not affect the weight of primal cuts in the right-side half-carcass. Compared to Pulawska pigs, crossbreds featured a significantly lower weight of bacon and ribs (by 1.17 kg) but a higher (p ≤ 0.05) weight of fillet (by 1.0 kg) and ham with shin (by 0.43 kg). The diet had no impact on the evaluated muscles, except a reduction (by 2.3 points) in colour lightness (L) of musculus semimembranosus in fattening pigs receiving feed rations with a 60% share of oats. Both muscles in Pulawska pigs showed significantly better (p ≤ 0.05) water-holding capacity and reduced colour lightness (L) in comparison to crossbred pigs. Moreover, the longissimus lumborum muscle of Pulawska pigs had a higher chroma (C) and a lower hue (H). The contents of essential nutrients in the evaluated muscles did not depend on the pigs’ diet and breed, except that a higher by 0.3% (p ≤ 0.05) intramuscular fat content was found in the longissimus lumborum muscle of Pulawska pigs. Neither of the experimental factors showed significant impact on the total saturated fatty acids (SFA) and total unsaturated fatty acids (UFA), or on neutral or hypocholesterolemic (DFA) and hypercholesterolemic fatty acids (OFA) in the evaluated muscles. The muscles of Pulawska x PLW pigs contained more (by 1.77% FA in the longissimus lumborum and 1.16% in the semimembranosus) polyunsaturated fatty acids (PUFA) than the muscles of the Pulawska breed (p ≤ 0.05). In addition, naked oats included in the pigs’ diet increased (p ≤ 0.05) the share of PUFA in intramuscular fat. The muscles of Pulawska pigs, in comparison to the muscles of crossbreds, showed a significantly improved ratio: by 66% and 69% in the longissimus lumborum and the semimembranosus muscles, respectively. To sum up, a 60% inclusion of naked oats is recommended in the rations of both Pulawska pigs and Pulawska x PLW crossbreds, since it allows satisfactory carcass composition and meat quality to be maintained. Pulawska pigs had worse productivity ratios but showed improved meat quality traits in terms of the n-6/n-3 ratio.

Genome-wide approaches delineate the additive, epistatic, and pleiotropic nature of variants controlling fatty acid composition in peanut (Arachis hypogaea L.).

The fatty acid composition of seed oil is a major determinant of the flavor, shelf-life, and nutritional quality of peanuts. Major QTLs controlling high oil content, high oleic content, and low linoleic content have been characterized in several seed oil crop species. Here, we employ genome-wide association approaches on a recently genotyped collection of 787 plant introduction accessions in the USDA peanut core collection, plus selected improved cultivars, to discover markers associated with the natural variation in fatty acid composition, and to explain the genetic control of fatty acid composition in seed oils. Overall, 251 single nucleotide polymorphisms (SNPs) had significant trait associations with the measured fatty acid components. Twelve SNPs were associated with two or three different traits. Of these loci with apparent pleiotropic effects, 10 were associated with both oleic (C18:1) and linoleic acid (C18:2) content at different positions in the genome. In all 10 cases, the favorable allele had an opposite effect-increasing and lowering the concentration, respectively, of oleic and linoleic acid. The other traits with pleiotropic variant control were palmitic (C16:0), behenic (C22:0), lignoceric (C24:0), gadoleic (C20:1), total saturated, and total unsaturated fatty acid content. One hundred (100) of the significantly associated SNPs were located within 1000 kbp of 55 genes with fatty acid biosynthesis functional annotations. These genes encoded, among others: ACCase carboxyl transferase subunits, and several fatty acid synthase II enzymes. With the exception of gadoleic (C20:1) and lignoceric (C24:0) acid content, which occur at relatively low abundance in cultivated peanuts, all traits had significant SNP interactions exceeding a stringent Bonferroni threshold (α = 1%). We detected 7682 pairwise SNP interactions affecting the relative abundance of fatty acid components in the seed oil. Of these, 627 SNP pairs had at least one SNP within 1000 kbp of a gene with fatty acid biosynthesis functional annotation. We evaluated 168 candidate genes underlying these SNP interactions. Functional enrichment and protein-to-protein interactions supported significant interactions (P-value < 1.0E-16) among the genes evaluated. These results show the complex nature of the biology and genes underlying the variation in seed oil fatty acid composition and contribute to an improved genotype-to-phenotype map for fatty acid variation in peanut seed oil.

Daniellia oliveri Leaf Extracts as an Alternative to Antibiotic Feed Additives in Broiler Chicken Diets: Meat Quality and Fatty Acid Composition

The objective of the study was to examine the effect of feeding different levels of Daniellia oliveri leaf extract (DOFE) on the meat quality and fatty acid composition of broiler chicks. A total of 375 one –day old broiler chicks were randomly assigned into five dietary treatments of seventy-five birds per group; each group was further divided into 5 replicates consisting of 15 chicks each. The dietary treatments include a control diet-fed 1.25 g/litre Oxytetracycline (T1), T2, T3, T4 and T5 were fed DOFE at 20 ml, 40ml, 60ml and 80 ml/liter respectively. Basal diet was formulated to meet the nutritional requirements of birds according to NRC (1994), feed and water were given ad libitum and the experiment lasted for 56 days. Result obtained showed that Daniellia oliveri leaf contained Dry matter (89.11%), crude protein (18.95%), crude fibre (13.11%), ether extract (4.78%), ash (6.10%), neutral detergent fibre (28.10%), and acid detergent fibre (47.50%). Significant differences (P&lt;0.05) were observed in the proximate composition of the breast meat. Total saturated fatty acid (TSFA), total unsaturated fatty acid (TUFA), and omega-6/omega -3 ratio (n-6: n-3) values were significantly influenced (P&lt;0.05) by DOFE. Birds in T5 had the highest TUFA value of 77.87 % followed by T4 (72.45%), T3 (66.43%), T2 (61.94 %), and T1 (41.47%) respectively. While T1 (44.71%) had the highest value of TSFA (P&lt;0.05) relative to other treatments. The atherogenic index was significantly (P&lt;0.05) different among the treatments, the value increases as the level of DOFE increased. It was concluded that feeding DOFE to birds at 80 ml/liter highly influenced the composition of fatty acid and meat quality of animals without any negative effect on their general performance.

Comparative study of the nutritional characteristics and fatty acid profiles of the seeds and seed oils of sweet, bell and bird varieties of pepper (capsicum species)

The proximate compositions of the seeds and fatty acid contents of the seed oils of the three varieties of Capsicum (pepper) species fruits – sweet, bell and bird pepper – have been determined using standard AOAC methods. Their proximate compositions gave moisture contents as 82.54%, 83.32% and 84.74% for bird, sweet and bell peppers respectively. Other proximate values in the various pepper samples ranged as follows: crude fat 1.52%–2.21%, crude protein 2.64%–3.51%, crude fibre 2.72%–4.71%, ash contents 1.62%–3.03% and carbohydrate contents 4.52–6.96%. The order of abundance of the fatty acids were linoleic &gt; oleic &gt; stearic &gt; palmitic in each sample. All the other fatty acids, except myristic acid in bell pepper seed oil, had values lower than 1.00% in all samples. The total unsaturated fatty acids predominated the total saturated ones with values ranging from 79.23% in bird pepper to 82.33% in bell pepper. The total polyunsaturated fatty acids ranged from 57.80% in sweet pepper to 66.50% in bell pepper while the total essential fatty acids ranged from 57.78% in sweet pepper to 66.00% in bell pepper. The total unsaturated/saturated (P/S) ratio was highest in bell pepper, making it the most nutritionally useful of them all.  Â

The changes of chemical composition of microencapsulated roselle (Hibiscus sabdariffa L.) seed oil by co‐extrusion during accelerated storage

AbstractRoselle seed oil (RSO) is an underutilised seed oil, which is having beneficial properties to humans. Microencapsulation of vegetables and seed oil is an alternative to preserve these properties and deliver them to the human diet. In this study, microencapsulation of RSO was performed using co‐extrusion technology and 1.5% w/w sodium alginate solution with 1.5% w/w high methoxyl pectin as the wall materials. Results showed that the microencapsulation efficiency was high (95.68 ± 1.95%) and the microencapsulation indeed preserved the content of unsaturated fatty acids, phytosterols, and tocopherols. During an accelerated storage condition at 65 °C for 24 days, most of these properties remained high for the first six storage days, including tocopherol which preserved high γ‐tocopherol. Worth noting that the microencapsulated RSO (MRSO) was particularly effective in preserving the total unsaturated fatty acids (especially C18:1 and C18:2), even during the 24th day of storage. The total unsaturated fatty acids retained by MRSO were significantly (P &lt; 0.05) higher (70 ± 0.4%) than the RSO (65.6 ± 1.6%). In short, microencapsulation was effective in preserving the RSO properties.

Soybean Oil Replacement by Poultry Fat in Broiler Diets: Performance, Nutrient Digestibility, Plasma Lipid Profile and Muscle Fatty Acids Content

Simple SummaryThe effect of partial or complete substitution of soybean oil (SO) by poultry fat (PF) on growth, nutrient digestibility, plasma lipids, and the pectoral muscle content of fatty acids (FAs) was examined in this study. Dietary PF supplementation improved breast muscle FA profile but did not affect muscle vitamin E content and liver thiobarbituric acid reactive substances (TBARS). By adding PF to the diet, economic efficiency was greatly improved in a dose-dependent manner. Therefore, the results of this study revealed that PF could be used as a partial or total replacement of SO in broiler nutrition without affecting their performance or physiological response with a tendency to improve their meat products.Continuous genetic improvements of commercial broiler strains has led to the necessity of using fats in their rations to fulfill a large portion of the energetic requirements. Several fat sources have been introduced in poultry nutrition, such as rendering poultry fat (PF) an available and cheap lipid source compared to conventional sources such as soybean oil (SO). The present study investigated the effect of partial or full replacement of SO by PF on performance, nutrient digestibility, blood lipids, and fatty acids (FAs) content of pectoral muscle. Four hundred and eighty one-day-old male Ross-308 chicks were distributed into four experimental groups (12 replicates each): the first group (control) was fed a diet formulated with soybean oil as a fat source while the second to fourth groups (PF25, PF50, and PF100) were fed diets formulated with 25, 50 and 100% of PF as a fat source instead of SO. Results revealed no synergistic effect between SO and PF in any of the studied parameters. Replacing SO by PF did not alter birds’ growth, carcass characteristics, and plasma indices of birds. Abdominal fat% was increased (p < 0.01) in PF50 and PF100. Dry matter digestibility was improved (p < 0.05) in PF50 and PF100, while crude fat and protein digestibility was not affected. Contents of palmitic and docosahexaenoic acids in the pectoral muscle of PF50 and PF100 were reduced (p < 0.01) while concentrations of oleic and linolenic acids, total unsaturated FAs, and polyunsaturated FAs/Saturated FAs ratio were elevated (p < 0.05) in the same groups. Liver thiobarbituric acid reactive substances (TBARS) and muscle vitamin E contents were not altered. The dietary addition of PF greatly improved economic parameters. In conclusion, PF can be used as a lipid source in broiler diets to produce inexpensive meat while maintaining its growth performance.

Physicochemical attributes, oxidative stability, and microbial profile of boneless sirloin and bone-in T-bone steaks from Hanwoo steer with reference to dry-aging.

We investigated the comparative physicochemical attributes, oxidative stability, and microbial characteristics of 28 days dry-aged meat in between boneless sirloin (gluteus medius) and bone-in T-bone steaks (infraspinatus) muscles from Korean Native Hanwoo Steer (KNHS). Results reveal that regardless of the muscles, dry-aging increased protein content and water-holding capacity (WHC) (p < 0.05). Meat from infraspinatus-aged muscle led to darker meat with higher pH values than un-aged meat (p < 0.05). However, fat content, CIE a*, and CIE b* remained unchanged in both muscles at aging. At aged meat, thiobarbituric acid reactive substances (TBARS) values from bone-in infraspinatus muscle was 2.5-fold higher than boneless gluteus medius muscle (p < 0.05). Dry-aging led to an increase in the contents of total unsaturated fatty acids (UFAs), monounsaturated fatty acids (MUFAs), and UFA/saturated fatty acids (SFA) in both muscles (p < 0.05). Furthermore, gluteus medius aged muscle concentrated with olic acid (C18:1) compared to infraspinatus aged muscle. Irrespective of the muscles, dry-aging enhanced the total free amino acids (FAAs) as well as tasty, and bitter amino acid contents whereas decreased the tasty/bitter amino acids (p < 0.05). Aromatic amino acid, tryptophan that converted to serotonin was 2-fold higher in boneless gluteus medius muscle than bone-in infraspinatus muscle at pre and post aging processes (p < 0.05). Aged Infraspinatus muscle increased total bacteria (p < 0.05) while no salmonella spp. was detected in both muscles. Taken together, our study confirms that 28 days dry-aging profiling the quality characteristics of boneless sirloin (gluteus medius) and bone-in T-bone steaks (infraspinatus) distinctly while gluteus medius aged steak performs better owing to oxidative stability and functional compounds than infraspinatus aged steak.

Development and Physicochemical Properties of Low Saturation Alternative Fat for Whipping Cream.

We developed an alternative whipping cream fat using shea butter but with low saturation. Enriched stearic-oleic-stearic (SOS) solid fat was obtained from shea butter via solvent fractionation. Acyl migration reactant, which mainly contains asymmetric SSO triacylglycerol (TAG), was prepared through enzymatic acyl migration to obtain the creaming quality derived from the β’-crystal form. Through enzymatic acyl migration, we obtained a 3.4-fold higher content of saturated-saturated-unsaturated (SSU) TAG than saturated-unsaturated-saturated (SUS) TAG. The acyl migration reactant was refined to obtain refined acyl migration reactant (RAMR). An alternative fat product was prepared by blending RAMR and hydrogenated palm kernel oil (HPKO) at a ratio of 4:6 (w/w). The melting points, solid fat index (SFI), and melting curves of the alternative products were similar to those of commercial whipping cream fat. The alternative fat had a content of total unsaturated fatty acids 20% higher than that of HPKO. The atherogenic index (AI) of alternative fat was 3.61, much lower than those of whipping cream fat (14.59) and HPKO (1220.3), because of its low atherogenic fatty acid content and high total unsaturated fatty acids. The polymorphic crystal form determined by X-ray diffraction spectroscopy showed that the β’-crystal form was predominant. Therefore, the alternative fat is comparable with whipping cream that requires creaming quality, and has a reduced saturated fat content.

Chlorella vulgaris Microalgae and Copper Mixture Supplementation Enhanced the Nutrient Digestibility and Milk Attributes in Lactating Boer Goats

Abstract Thirty-two lactating Boer goats (35.2 ± 1.4 kg body weight) were grouped into control and three treatment groups in completely randomised design. In treatment groups, supplementation was done as a mixture of 5 g Chlorella vulgaris + 4.5 mg of supplemental Cu/kg diet from CuSO4 (Alg5 treatment), 10 g C. vulgaris + 9 mg of supplemental Cu/kg diet (Alg10 treatment), or a mixture of 15 g C. vulgaris + 13.5 mg of supplemental Cu/kg diet (Alg15 treatment). Treatments did not affect feed intake; however, Alg10 treatment increased (P&lt;0.001) nutrient digestibility. Treatments did not affect ruminal pH, ammonia-N, butyrate; however, the Alg10 treatment increased (P&lt;0.01) ruminal total volatile fatty acids, propionate and acetate concentrations. Without affecting other blood measurements, the Alg10 treatment quadratically increased (P&lt;0.001) serum glucose and Cu. The Alg10 treatments increased (P&lt;0.001) daily milk production and the concentration of fat, and enhanced milk (feed) efficiency. The Alg10 treatment decreased (P&lt;0.05) milk saturated fatty acids and the atherogenic index, and increased the proportions of total conjugated linoleic acids, C18:1n9t, odd fatty acids and total unsaturated fatty acids compared with the control treatment. Present study concluded that inclusion of a mixture of 10 g C. vulgaris + 9 mg Cu/kg diet in the diet of lactating Boer goats enhanced nutrient digestibility, ruminal fermentation, milk production, feed efficiency as well as milk nutritive value. Increasing the dose of the mixture to 15 g C. vulgaris + 13.5 mg Cu/kg diet is not recommended in the diet of lactating Boer goats.

Quality characteristics of extracted oil from the head and gills of Catfish and Titus fish

BackgroundThe research was carried out to assess the lipid characteristics of oil extracted from the head and gills of Claris macrocephalus and Scomber scombrus.ResultsThe results confirmed the presence of C16:0, C18:0, C18:1, C18:2, C18:3, C22:6 as the major fatty acids. Titus fish head (TFHD) oil had a total saturated fatty acid (TSFA) of 40.804 ± 0.002% of which C16:0 and C18:0 were the most dominant fatty acids present while the total monounsaturated fatty (TMUFA) acids present in the oil was 33.853 ± 0.001%. The total polyunsaturated fatty acid (TPUFA) content of the TFHD oil sample was found to be 25.368 ± 0.002%. The results revealed that the total unsaturated fatty acid (TUFA) present in the TFHD was higher than the TSFA. The results obtained for Titus fish gills (TFGL) oil sample followed the same trend as observed in the head sample. The results showed that the four (4) oil samples contain high level of palmitic acid (C16:0) and stearic acid (C18:0) both of which are saturated fatty acids (SFA). The main MUFA identified was C18:1 (cis-6) and ranged from 14.120 ± 0.004 to 20.418 ± 0.002% for TFHD, TFGL, CFHD, CFGL and oleic acid (C18:1) ranged from 10.297 ± 0.001 to 11.570 ± 0.003% for TFHD, TFGL, CFHD and CFGL.ConclusionsOils of CFHD and TFHD showed higher peroxide values indicating suitability for human consumption.

Protective Effect of Moringa Oleifera Seeds Extract on Toxicity In Liver And Kidney Induced By Acetampride .

The present study aimed to distinguish light on the protective of Moringa oleifera seeds dried ethanolic extract on the toxicity in liver and kidney induced by acetamprid pesticide which widely produced and used for agricultural treatment in Egypt. Physico-chemical parameters and identification of fatty acid composition of Moringa oleifera seeds oil were elvaluated. In addition , the biological effects of bioactive components of seeds dried extract at concentration 200 mg/kg b.w. oil as additives into the diets on kidney and liver functions , lipid profile ,serum glucose levels , hematological parameters and histopatholgical examination of experimental rats were evaluated. From the obtained results , it could be noticed that the total phenolic acid , flavonoids and tannin contents in Moringa oleifera seeds of the dried ethanolic extract were 44.25±1.13 , 114.36±0.72 and 20.77±1.10 ug/g , respectively. Total antioxidant capacity of dried ethanolic extract of Moringa oleifera seeds was found to be 143.29±0.37 , 178.10±2.18 , 189.18±2.06 and 222.47±0.81 at 50 , 100 , 150 and 200 ug/ml . Also, the results showed that the Moringa oleifera (MO) seeds oil extract was dominated by the high percentage of total unsaturated fatty acid (80.29%) than its saturated (19.71%). On the other hand , the results showed that there were significant increasment of biochemical parameters such as serum urea and creatinine (as function of kidney) , as well as ALT and AST activity also albumin and total protein (as function of liver) , total cholesterol , triglyceride and serum glucose levels of the experimental rats. These values were found significantly decreased in all treatments rats fed diets containing MO seeds oils extract at dose of 200 mg /ml b.w. as compared with the group treated by acetampride (toxicated group).The results of the effects of MO seeds extract on hematological parameters of intoxicated rats caused significant decreasments in total white blood cells counts (WBCs) . While, MCV , MCH and MCHC were significantly unchanged relative to those of different from the control group but haemglobin (Hb) and red blood cells (RBCs) was increased. Histopathological studies confirmed chemical analysis results. Finally, from the obtained results and histopathological examination , it could be concluded that the administration of MO seeds dried extract of ethanolic oils might improve the liver and kidney functions in the present experimental rats.

Feeding System Resizes the Effects of DGAT1 Polymorphism on Milk Traits and Fatty Acids Composition in Modicana Cows.

Simple SummaryGenetic selection for single-locus polymorphisms could offer suitable opportunities to rapidly improve milk traits in local unselected cattle breeds characterized by low production levels. Since these hardy breeds are generally raised in traditional extensive and semi-intensive systems, which make wide use of grazing resources, the interactive effect between genotype and feeding system is worthy of investigation. In Modicana cattle breed, milk composition and fatty acid profile were influenced by both genetic polymorphisms at the DGAT1 K232A locus and feeding systems. The milk from homozygous AA cows was associated with a more favorable fatty acid composition due to a lower percentage of total saturated fatty acids, saturated to unsaturated ratio, atherogenic index, and a greater presence of oleic acid and total unsaturated fatty acids. Our finding confirmed the important role of pasture feeding on milk composition: the high nutritional and healthy value of milk obtained in extensive systems by pasture-fed cows. The interaction between the two experimental factors also appears to play a role: in our experimental condition, it seems that high pasture feeding can resize the effect of the DGAT1 genotype on milk traits and fatty acid composition in Modicana cows.The interaction between genetic polymorphism and feeding system on milk traits and fatty acid composition was investigated in Modicana cows. Two DGAT1 K232A genotypes (AK and AA) and two feeding regimes, extensive system (EX) with 8 h of grazing without concentrate (EX) and semi-intensive systems (SI) with 2 h of grazing with concentrate, were investigated. DGAT1 genotype did not influence milk yield and composition. The feeding system affected milk composition: protein was significantly higher in SI and lactose in the EX system. A significant genotype × feeding system interaction was observed: the protein and casein levels of AK cows were higher in the SI compared to the EX system. Milk fatty acids profile, total saturated to total unsaturated fatty acids, n-6 to n-3 ratios, and atherogenic index were affected by the feeding system, improving the healthy properties of milk from animals reared in the extensive system. DGAT1 genotype influenced the fatty acid composition: milk from AA cows had a more favorable fatty acid composition due to lower total saturated fatty acids, saturated to unsaturated ratio, atherogenic index, and higher levels of oleic acid and total unsaturated fatty acids. Furthermore, an interaction genotype x feeding system was observed: the AK milk was richer in short-chain FAs (C4:0–C8:0) and C10:0 only in the EX but not in the SI system. Our data suggest that a high amount of green forage in the diet of Modicana cows can resize the effect of the DGAT1 genotype on milk traits and fatty acids composition.

Nitric oxide donor sodium nitroprusside-induced transcriptional changes and hypocrellin biosynthesis of Shiraia sp. S9

BackgroundNitric oxide (NO) is a ubiquitous signaling mediator in various physiological processes. However, there are less reports concerning the effects of NO on fungal secondary metabolites. Hypocrellins are effective anticancer photodynamic therapy (PDT) agents from fungal perylenequinone pigments of Shiraia. NO donor sodium nitroprusside (SNP) was used as a chemical elicitor to promote hypocrellin biosynthesis in Shiraia mycelium cultures.ResultsSNP application at 0.01–0.20 mM was found to stimulate significantly fungal production of perylenequinones including hypocrellin A (HA) and elsinochrome A (EA). SNP application could not only enhance HA content by 178.96% in mycelia, but also stimulate its efflux to the medium. After 4 days of SNP application at 0.02 mM, the highest total production (110.34 mg/L) of HA was achieved without any growth suppression. SNP released NO in mycelia and acted as a pro-oxidant, thereby up-regulating the gene expression and activity of reactive oxygen species (ROS) generating NADPH oxidase (NOX) and antioxidant enzymes, leading to the increased levels of superoxide anion (O2−) and hydrogen peroxide (H2O2). Gene ontology (GO) analysis revealed that SNP treatment could up-regulate biosynthetic genes for hypocrellins and activate the transporter protein major facilitator superfamily (MFS) for the exudation. Moreover, SNP treatment increased the proportion of total unsaturated fatty acids in the hypha membranes and enhanced membrane permeability. Our results indicated both cellular biosynthesis of HA and its secretion could contribute to HA production induced by SNP.ConclusionsThe results of this study provide a valuable strategy for large-scale hypocrellin production and can facilitate further understanding and exploration of NO signaling in the biosynthesis of the important fungal metabolites.

Effect of Production System (Intensive and Free-Range), Slaughter Age and Gender on Nutrient and Fatty Acid Composition of Meat in Partridges (Alectoris chukar)

This study was conducted to determine the effect of production system, slaughter age, and gender on the nutrient composition, fatty acids profile, and index values of breast and thigh meat in Alectoris chukar partridges. Partridges were slaughtered at 14, 16, and 18 weeks of age and skinless breast and thigh meat of male and female were used in the study (a total of 96 samples in 2 replicates). The production system affected only the crude fat level of the thigh meat in terms of nutrient composition and it was found higher in the intensive system compared to the free-range system. Age and gender did not significantly affect the composition of breast and thigh meat. In terms of fatty acid profile, erucic acid (C22:1n9) level in breast meat was higher in intensive system, while it was higher in thigh meat in free-range. While the percentage of docosahexaenoic acid (C22:6n3) increased with age in breast meat, it decreased in thigh meat with age. While eicosenoic acid (C20:1) percentage was higher in breast meat of male birds compared to females, only stearic acid (C18:0) was found to be higher in thigh meat. Saturated fatty acids (SFA), linolenic acid (n3), thrombogenic index (TI) and atherogenic index (AI) values were higher in thigh meat produced in free-range system, while polyunsaturated fatty acids (PUFA), total unsaturated fatty acids (UFA), linoleic acid (n6) and hypocholesterolaemic / hypercholesterolaemic ratio (h/H) were significantly higher in intensive system. While SFA and TI levels in thigh meat decreased with age, monounsaturated fatty acids (MUFA), UFA and oleic acid (n9) percentages increased. MUFA / SFA in thigh meat of females was higher than males, other indexes were found insignificant. The results shows that partridges had desirable fatty acid composition. Especially, the increase in MUFA and UFA values with age in thigh meat compared to breast indicates that thigh meat is enriched in terms of unsaturated fatty acids. However, the higher SFA and AI values obtained in the free-range system could be considered a negative outcome for alternative production systems that prioritize bird welfare and consumer demands.