Assessing the impact of river water on concrete properties is an important aspect of the construction industry, especially in regions with significant exposure to riverine environments, like Bangladesh. This study investigated the applicability of river water in concrete manufacturing regarding its compressive strength and density. Water samples were collected from two major rivers (the Padma and the Shitalakshya) in Bangladesh and analyzed for thirty water quality parameters. 168-cylinder specimens were cast and tested for compressive strength after curing for 7, 14, and 28 days. The investigation was also carried out with two different coarse aggregates (brick and stone chips) in the mix design. Curing was performed with fresh and river water separately. Therefore, ten different experimental conditions were explored. The compressive strength of concrete using river water compared to freshwater decreased from 0 to 24%, except for three cases, where strength increased by 4.2%, 7.3%, and 8%. The variation in water quality between the two rivers significantly influenced the reduction in compressive strength. Higher values of pH, total suspended solids, total solids, ammonia, total hardness, alkalinity, and conductivity in the Padma River water led to a greater reduction in compressive strength than using the Shitalakshya River water. The type of coarse aggregate used also had an impact on strength. Both rivers’ water met the concrete production standards; nevertheless, a pre-treatment process is recommended. Successfully using river water in concrete production could reduce the demand for freshwater resources, contributing to the construction industry’s sustainability.