Total Input Research Articles

The distribution and structural plasticity of excitatory synapses along interneuron dendrites in the adult hippocampus

Hippocampal interneurons form a large, heterogeneous group with differing structural, physiological, and chemical phenotypes. Most CA1 interneuron dendrites, however, lack spines. To investigate structural synaptic plasticity along interneuron dendrites, we generated three-dimensional (3D) reconstructions from serial section electron microscopy (EM) of excitatory synapses along aspiny dendrites in CA1 stratum radiatum of young adult rats under control conditions and at 2 h during LTP induced with theta-burst stimulation. In total, 43 aspiny dendritic segments and their synapses were identified and reconstructed in 3D. Aspiny dendrites were categorized as smooth or varicose based on dendritic volume contours along each segment. We further sub-segmented varicose dendrites varicosities and inter-varicose regions using automated and manual methods. We found synapses and total synaptic input per length were distributed uniformly along smooth dendrites. Synapses along varicose dendrites, however, occurred preferentially at varicosities. While synaptic input per length along varicosities was similar to that along smooth dendrites, both synapse number and total synaptic input per length along inter-varicose regions were lower. Dendritic segments were also analyzed for mitochondria and glycogen content. We found that mitochondria and glycogen were also distributed uniformly along smooth dendrites. In varicose dendrites, however, mitochondrial volume and glycogen granule numbers were highest in varicosities, while inter-varicose regions were comparatively devoid of these resources. The spatial distribution of synapses and dendritic resources in both smooth and varicose dendrites was preserved at 2 h during LTP when compared to control conditions. These findings suggest that synapses occur along interneuron dendrites where dendritic resources are most available. When dendritic resources are distributed uniformly throughout the dendrite, as occurs in smooth dendrites, synapse distribution is also uniform. Varicosities, on the other hand, represent resource-rich sites along varicose dendrites where synaptic clustering occurs. This work was supported by the National Science Foundation (NSF 1707356, NSF 2014862) and the National Institutes of Health (R01 5R01MH09598). This is the full abstract presented at the American Physiology Summit 2024 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

High Efficiency Operation of a 4K-Gifford-McMahon Cryocooler without Rotary Valve with a Metal Bellows Compressor

Traditional Gifford-McMahon cryocoolers are operated with an integrated rotary valve which regulates the compressed Helium supply and return into and from the cold head. Theory predicts that about 50% of the input energy is lost in this rotary valve [1]. In this work we removed the rotary valve from a Sumitomo RDK-101G cold head and drove the Gifford-McMahon cryocooler in Stirling-mode directly with a slow-moving metal bellows compressor at around 1.2Hz. We could demonstrate that the energy efficiency of the cryocooler system improves by almost 50%. As a result, we were able to operate this cold head with 650W of total electrical input power with a cooling power of 140mW at 4.2K on the second stage and no-load temperatures of below 40K on the first stage. This corresponds to a COP of 215mW/1000W which one of the highest COPs reached for small and medium size 4K GM and pulse tube cryocoolers.

Performance and Degradation of Electrolyte Supported SOECs with Advanced Thin-Film Gadolinium Doped Ceria Barrier Layers in Long-Term Stack Test

Electrolyte supported Solid Oxide Cells (ESCs) with advanced thin-film Gd-doped ceria diffusion barrier layers between electrolyte and electrodes were assembled and electrochemically investigated in steam electrolysis mode in a so-called “rainbow” stack with 30 repeat units (RUs). The barrier layers were deposited onto the electrolyte supports via electron-beam physical evaporation deposition (EB-PVD) method at 600 °C. In this paper, the investigation mainly focuses on the electrochemical characteristics of RUs containing the EB-PVD thin-film GDC layers. At the initial stage of the SOEC operation, the stack reached a high performance with an electrical efficiency of 99.65% at 75% steam conversion and a total power input of 1.98 kW. A long-term stack test was performed in SOEC mode for over 5000 h and demonstrated a low voltage degradation of approx. +11.3 mV·kh–1 per RU (+0.9% kh–1). The RUs with EB-PVD GDC thin-films revealed similar initial performance and degradation rate to the state-of-the-art cells with screen printed GDC layers.

The effect of restricting groundwater exploitation on agriculture: Evidence from Huang-Huai-Hai region, China

Food security and groundwater conservation may conflict with each other due to high irrigation demands. This study aims to identify how groundwater conservation policies affect agriculture and the mechanisms of long-term adaptation. Our framework aggregates the stochastic frontier analysis, dynamic panel fixed effects model, and long-difference approach to evaluate the impact of restricting groundwater exploitation on agricultural total factor productivity, agricultural inputs, and outputs in the Huang-Huai-Hai area of China. The results demonstrate that, in the short run, the reduction in groundwater exploitation has a negative impact on agricultural total factor productivity, fertilizer and machines but promotes water input, which results in a more negative effect on agricultural output measured by yield. In the long term, restricting groundwater exploitation still implies a loss of agriculture. However, the long-run adaptation offsets 75% and 64% of the short-run effects on total factor productivity and yield, respectively. Despite more flexible adjustment in fertilizer and machines, long-term adaptation mitigates yield losses to a lesser extent than TFP due to water use constraints.

A Study on Determination of Energy Productivity and Greenhouse Gas Emissions in Wheat Production

In this study, energy use efficiency (EUE) and greenhouse gas emissions (GHG) in wheat production were defined, and the energy equivalents (EE) of the inputs in production per unit production area, EUE and GHG values of the product were computed. The data used in the study were obtained from 175 different wheat producing enterprises in 2021 by conducting face-to-face surveys according to the proportional sampling method. In the study, the amount of direct (DE) and indirect energy (IE) use in wheat production and their shares in total energy consumption were defined. According to the results of the study, total energy input (EI) in wheat production was com-puted as 19,024.21 MJ/ha and energy output (EO) as 80,585.40 MJ/ha. It was defined that the input with the highest energy consumption was fertili-zation with a value of 8748.38 MJ/ha. This was followed by seed energy input 4626.79 MJ/ha (24.32%), fuel energy 2697.25 MJ/ha (14.18%), irriga-tion energy 2362.50 MJ/ha (12.42%), chemicals energy 269.19 MJ/ha (1.41%), machinery energy 309.52 MJ/ha (1.63%), human labor energy 10.58 MJ/ha (0.06%). EUE, energy productivity (EP), specific energy (SE) and net energy (NE) yield values were 4.24, 0.29 kg/MJ, 3.47 MJ/kg and 61561.19 MJ/ha, respectively. Total GHG emission for wheat production was computed as 3784.60 kgCO2-eq/ha. The highest share of total GHG emissions belonged to seed (59.41%). Seed was followed by irrigation (16.84%), nitrogen fertilizer use (14.60%), phosphate fertilizer use (3.99%), fuel use (3.49%), chemicals use (0.98%), machinery use (0.58%) and hu-man labor (0.10%). In addition, the GHG ratio in wheat production was computed as 0.69 kgCO2-eq/ha.

Investigation of mechanical properties in thin spray-on liner coated sandstone: Comparative study of unfilled and filled flaws

Thin spray-on liners (TSLs) are increasingly recognized as a potent skin support technology in underground engineering applications, particularly in mining. To better understand the coating and filling effects of TSL on the mechanical properties of the flawed specimens, a series of uniaxial compression tests were conducted on TSL-coated sandstone with unfilled and filled flaws. The experimental results showed that compared with the flawed specimen, the strength and peak strain of the TSL-coated and filled specimens increased, and the degree of improvement was more evident for the filled specimen coated with TSL. The specimens coated with TSL exhibited higher total, elastic, and dissipation energies compared to the uncoated specimens. Additionally, a greater proportion of the total input energy was transformed into dissipation energy after coating. For the filled specimens, more dissipation energy was generated during the failure process owing to the friction of the flaw surface provided by the filling effect. Moreover, the crack initiation and propagation behaviors of the flawed and filled sandstone specimens coated with TSL were analyzed by numerical simulation. For the specimens with an inclination angle of 45°, the TSL provided confinement to inhibit the initiation and propagation of tensile cracks, resulting in delayed crack initiation. The TSL filling plays a role in sharing part of the stress on the flaw surface, which weakens the stress concentration at the flaw tip. The effect of the TSL on the stress at the flaw tip was analyzed using a theoretical solution. The variation in SIF KII of the specimens with different flaw inclination angles was studied using a theoretical solution. Compared with the flawed specimens, the stress concentration in the filled flawed specimen was lower and the confinement effect could enhance the decrease in the flaw tip stress.

Dynamic Energy Use Efficiency, Carbon Input, and Agricultural Benefits of Multiple Cropping in Southern China—A Case Study from Guangdong Province

Background: With the purpose of exploring the development of new quality productive forces in Guangdong, the present study hypothesized that reducing energy and carbon inputs was beneficial for increasing Guangdong’s multi-cropping agricultural energy output and economic returns. Methods: The energy use efficiency of crop production and the corresponding carbon input and agricultural benefit trends from 2011 to 2021 were examined by linear regression analysis for Guangdong Province, China. The corresponding development trends were also predicted using a grey model. Results: The results indicated that the total energy output increased by 12.50%, wherein the energy productivity levels of peanuts, vegetables, mulberry, and fruits increased greatly (51.27–106.17%), while the total energy input and the total carbon input decreased by 12.17% and 6.37%, respectively. Moreover, the energy input per carbon input decreased by 6.19%, while the energy output per carbon input increased by 20.15%. Both energy-related indicators and economic-related indicators all had substantially increased (28.08–44.97% and 83.86–120.91%, respectively). Grey model predictions show that the agricultural output value increased steadily under the current agricultural policy of reducing fossil energy input. Conclusions: The current low-carbon and high-output agricultural model is beneficial for increasing Guangdong’s multi-cropping agricultural economic returns and mitigating greenhouse effects.

Ice base slope effects on the turbulent ice shelf-ocean boundary current

Abstract Efforts to parameterize ice shelf basal melting within climate models are limited by an incomplete understanding of the influence of ice base slope on the turbulent ice shelf-ocean boundary current (ISOBC). Here we examine the relationship between ice base slope, boundary current dynamics, and melt rate using 3-D, turbulence-permitting large-eddy simulations (LES) of an idealized ice shelf-ocean boundary current forced solely by melt-induced buoyancy. The range of simulated slopes (3-10%) is appropriate to the grounding zone of small Antarctic ice shelves and to the flanks of relatively wide ice base channels, and the initial conditions are representative of warm-cavity ocean conditions. In line with previous studies, the simulations feature the development of an Ekman boundary layer adjacent to the ice, overlaying a broad pycnocline. The time-averaged flow within the pycnocline is in thermal wind balance, with a mean shear that is only weakly dependent on the ice base slope angle α, resulting in a mean gradient Richardson number 〈Rig〉 that decreases approximately linearly with sinα. Combining this inverse relationship with a linear approximation to the density profile, we derive formulations for the friction velocity, thermal forcing, and melt rate in terms of slope angle and total buoyancy input. This theory predicts that melt rate varies like the square root of slope, which is consistent with the LES results and differs from a previously proposed linear trend. The derived scalings provide a potential framework for incorporating slope-dependence into parameterizations of mixing and melting at the base of ice shelves.

Adaptive fault-tolerant optimal control for hypersonic vehicles with state constrains based on adaptive dynamic programming

This paper addresses a novel adaptive fault-tolerant control (FTC) design based on adaptive dynamic programming (ADP) technique for hypersonic vehicle (HSV) subject to actuator fault and state constrains. The total control input is constructed by the combination of backstepping-based adaptive FTC and the ADP-based adaptive optimal control to improve the tracking performance and fault-tolerant capacity. By introducing a barrier Lyapunov function (BLF) to deal with the state constraints, a backstepping-based FTC scheme is designed to transform the tracking control problem into an equivalent optimal control problem. Subsequently, an adaptive optimal control strategy is developed by using the ADP technique to provide a supplementary control action. A critic network is constructed to solve the Hamilton–Jacobi–Bellman (HJB) equation online. The convergence properties of the closed-loop system are developed by utilizing the Lyapunov stability theory. Finally, simulation results are carried out to illustrate the effectiveness and efficiency of the proposed adaptive ADP-based FTC strategy.

Field and simulation studies on yield, water and nitrogen dynamics and use efficiency in rice-wheat crops in sequence

ContextThe dominant rice-wheat cropping system in the Indo-Gangetic plain faces challenges of water depletion and nitrogen pollution of groundwater as well as declining productivity caused by over irrigation and nitrogen fertilization by the farmers. To appreciate the apt use of irrigation water and nitrogen fertilizer in rice and wheat crops it is essential to explore the water and nitrogen balances as well as dynamics in these crops, involving complex interaction between weather, soil, irrigation water and nitrogen fertilizer management. ObjectiveThe objective of the study is to quantify yield, field water balance components, water use efficiency, water productivity, water footprint, and water and nitrogen dynamics of rice and wheat crops in relation to season, varieties, irrigation regimes and nitrogen levels. MethodsField experimentations were conducted in double split plot design along with calibrated and validated HYDRUS 1D model use for two years comprising of treatments in rice i.e. two varieties (PR122-V1 and PR126-V2), two irrigation regimes (2-days drainage period-I1 and soil water suction 16 kPa-I2) and two nitrogen levels (120-N1 and 90-N2 kg N ha−1); and in wheat, two varieties (Unnat PBW343-V1 and Unnat PBW550-V2), two irrigation (1.2 IW/CPE-I1 and 0.9 IW/CPE-I2) regimes and two nitrogen levels (120-N1 and 90-N2 kg N ha−1). ResultsThe highest grain yield recorded was 7.6 t ha−1 in treatment combination of V1I2N1 in rice and 5.4 t ha−1 in V1I1N1 in wheat owing to better plant growth and yield attributes (biomass production, number of effective tillers, panicle length, and 1000 grain weight). Total water footprints were lower in the treatment combination of V1I1N1 in rice and V1I2N1 in wheat. The water budget revealed a higher percent contribution of the total water input to drainage (62 %) in rice ensuing restoration of water in the soil profile; and to evapotranspiration (ET) (100 %) in wheat ensuing water depletion. Modeling water and nitrogen dynamics showed higher root water uptake in I2 treatment in rice and with I1 treatment in wheat; and leaching losses of NO3-N under rice than wheat; and under I2 treatment than I1 as well as in N1 than N2. ConclusionsThe V1I2N1 treatment in rice and V1I1N1 treatment in wheat demonstrated superior grain yield, soil water storage, and root water uptake. Conversely, V1I1N1 treatment in rice and V1I2N1 treatment in wheat showed higher water use efficiency and lower water footprint. SignificanceIt helps to evaluate the effectiveness of irrigation and nitrogen management strategies in maximizing crop productivity while minimizing environmental impacts.

Modeling 0.3 THz Coaxial Single-Mode Phase Shifter Designs in Liquid Crystals with Constitutive Loss Quantifications

This work proposes and examines the feasibility of next-generation 0.3 THz phase shifters realized with liquid crystals (LCs) as tunable dielectrics coaxially filled in the transmission line. The classic coaxial transmission line topology is robust to electromagnetic interference and environmental noise, but is susceptible to higher-order modes from microwave to millimeter-wave towards terahertz (THz) wavelength ranges, which impedes the low-insertion-loss phase-shifting functionality. This work thus focuses primarily on the suppression of the risky higher-order modes, particularly the first emerging TE11 mode impacting the dielectric loss and metal losses in diverse manners. Based on impedance matching baselines at diverse tuning states of LCs, this work analytically derives and models two design geometries; i.e., design 1 for the coaxial geometry matched at the isotopically referenced state of LC for 50 Ω, and design 2 for geometry matched at the saturated bias of LC with the maximally achievable permittivity. The Figure-of-Merit for design 1 and design 2 reports as 35.15°/dB and 34.73°/dB per unit length, respectively. We also propose a constitutive power analysis method for understanding the loss consumed by constitutive materials. Notably, for the 0.3 THz design, the isotropic LC state results in an LC dielectric loss of 63.5% of the total input power (assuming 100%), which becomes the primary constraint on achieving low-loss THz operations. The substantial difference in the LC dielectric loss between the isotropic LC state and saturated bias state for the 0.3 THz design (35.76% variation) as compared to that of our past 60 GHz design (13.5% variation) indicates that the LC dielectric loss’s escalating role is further enhanced with the rise in frequency, which is more pronounced than the conductor losses. Overall, the results from analytical and finite-element optimization in this work shape the direction and feasibility of the unconventional THz coaxial phase shifting technology with LCs, actioned as continuously tunable dielectrics.

Assessment of non-revenue water in the urban water distribution system network in Cameroon (Central Africa)

ABSTRACT This paper aims to assess non-revenue water (NRW) in the urban water distribution system in Cameroon. The methodological approach used in this study was based on online searches using the databases such as Google Scholar, Web of Science, PubMed, and the International Water Association. These online searches have been completed through field observations and interviews with institutional and non-institutional stakeholders in the urban water sector. Results indicate that the total volume of NRW in the urban area of Cameroon was 100.26 million m3/year, which represents 52.5% of the total system input volume. About 2 million people could be supplied by cutting Cameroon's NRW to half the present level. Findings also suggest that the water utility (Camwater) practices passive leakage control, meaning that Camwater repairs only those leaks that are visible. Reducing NRW can be achieved through leakage detection and pipe replacement, pressure management, water metering, and a program to identify, remove, and replace illegal connections.

INPUT OF DOMESTIC WASTE IN THE ESTUARY OF DUMAI RIVER AND MESJID RIVER INTO THE RUPAT STRAIT WATERS

The study was conducted in December 2022 in the Dumai River Estuary and Dumai City Mesjid River Estuary, Riau Province. The purpose of this study is to determine the amount and type of garbage that enters the sea through the estuary of the Dumai River and the Mesjid River, the difference between the amount and type of waste, and the difference between days from the garbage contained in the estuary of the Dumai River and the Mesjid River in Dumai City. The research method used is a direct survey method in the field, where data collection is carried out directly at low tide using a net stretched at the mouth of the river so that garbage is collected and trapped following the river's flow into the net. Based on the research conducted, it was found that the types of waste trapped were plastic, foamy plastic, cloth, paper, rubber, and wood. The amount of input and composition of waste in two weeks to the mouth of the Dumai River was 28,26 kg in the first week and 30,64 kg in the second week, while in the estuary of the Mesjid River, it was less, namely the first week as much as 20,59 kg, and in the second week as much as 19,44 kg. The difference between the amount and type of waste contained in the Dumai River and the Mesjid River for two weeks found that plastic waste occupied the most significant amount, namely 30,43 kg with a composition of 51,55% and 49,71 kg with a composition of 49,71% based on total waste input. The difference in waste input based on the day at the mouth of the Dumai River and the highest Mesjid River is found on Sunday, namely at the mouth of the Dumai River with a total waste of 27,79 kg and at the mouth of the Mesjid River with a total of 16,67 kg

Unveiling the potential of a functionalized pyrrole-based polymer for efficient cadmium ion removal from wastewater: synthesis, characterization, and performance evaluation

Cadmium ion Cd2+ contamination is a major environmental issue caused by industry. Polyarylidene N-hexane pyrrole (PAPh) and crosslinked polyarylidene N-phenyl pyrrole (PAPD) were prepared from the previously synthesized polymer (polyarylidene ketone (PAK)) by using the advantage of repeating carbonyl groups at the 1,4 position and reacting it with hexylamine and P-phenylenediamine via the Paal–Knorr reaction. Various methods were used to characterize polymers, such as FT-IR spectroscopy, X-ray diffraction (XRD), thermogravimetry analysis (TGA), UV–visible spectroscopy, scanning electron microscope (SEM), zeta potential, and surface area measurements (BET), revealing successful fabrication, good thermostability, and well-defined microporous structures useful for Cd2+ adsorption. Optimal adsorption capacities of 55.8 mg g−1 for PAPh and 86.95 mg g−1 for PAPD indicate a significant enhancement in Cd2+ adsorption via their microporous structures, Cd2+ adsorption was also investigated in terms of contact time, initial concentration, and pH. A total input concentration of 30 ppm Cd ions, may yield an 84.3% removal rate for PAPh and an 89.2% removal rate for PAPD. The experimental results were well-fit by many models, including pseudo-second-order kinetics (PSO), Freundlich isotherms, intraparticle diffusion, and Langmuir. The varying adsorption performances of the two polymers studied, (PAPh) and (PAPD), were found to be derived from their respective chemical structures, which include various functional groups, according to studies conducted on Cd2+ in an aqueous solution. Cd2+ adsorption on polymers was considered physisorption; π–cation interactions and surface complexation played significant roles in adsorption. The PAP polymers may be considered promising substitutes and innovative adsorbents to remove Cd2+ ions from a water solution.

Production of sustainable methanol from aquatic biomass via thermal conversion route

Energy security is considered a basic need for the community. The development of a non-competing energy source is necessary to maintain harmony when fulfilling energy and food needs. Aquatic biomass such as algae is a promising sustainable energy source as it can be cultivated in the sea or pond which does not require productive land. By using thermal reaction, algae can be converted into methanol via gasification followed by hydrogenation reactions. The key challenge of algae utilization lies in the high moisture content. This work presents an understanding of the role of moisture on the algae for methanol production. Although moisture content has a negative impact on the syngas quality, the methanol production does not change with increasing moisture content. Under the optimum condition, the algae-to-methanol route converts ∼45% of the total energy input into energy in the form of methanol. The production of one-ton methanol from algae with 10 wt% moisture requires algae (2.7 ton), oxygen (0.9 ton), boiler feed water (1.5 ton), thermal energy (12.1 GJ) and electricity (0.7 MWh). The present work provides a clear understanding of the potential of aquatic biomass for methanol production, leading to further development to achieve a feasible algae-to-methanol route.

Solute fluxes in headwater catchments with contrasting anthropogenic impact

Understanding the response of headwater catchments to increasing anthropogenic influences is one of the key issues in hydrological and geomorphological research. Two headwater catchments with contrasting human impacts on the environment were chosen to determine changes in surface water chemistry and solute fluxes. In one catchment, 22 % of the forest area was cleared in 2013 and in the other, 75 % of the arable lands was converted to grassland and forest in the last 25 years. Atmospheric input and solute mass balance were analysed using homogeneity Pettitt test. It seems that in both areas, a very important factor affecting solute fluxes was most likely changes, or lack thereof, in soil erosion. In the partially clear-cut catchment, a small clear-cut area and adequate protection of the soil cover had little effect on the stream water chemistry, and the total solute mass balance remained unchanged during the study period. In this catchment, the more pronounced effect on solute fluxes was using de-icing salts in winter, which contributed to a 62 % increase in total solute flux (mainly by supplying Na+ and Cl−, but also Ca2+, Mg2+ and K+). In a catchment with a decrease in arable lands, there was a 55 % decrease in total solute mass balance (including mass balance of Ca2+, Mg2+, Na+, K+, NH4+, SO4−, NO3−, Cl−) in the stream likely associated with a decrease in soil erosion and fertilization. In both catchments there was a decrease in total monthly atmospheric input (especially in input of NH4+, SO4− and NO3−) of 53 % and 21 %, respectively, over the study period. To summarize, the road salting has increased the amount of transported solutes in the stream and deteriorated surface water chemistry, while the reduction of arable land area has reduced the amount of transported solutes in the stream and improved surface water quality.

A holistic approach to refinery decarbonization based on atomic, energy and exergy flow analysis

As one of the largest industrial emitters, today’s refineries must take action to reduce emissions in response to global climate change and net-zero targets. However, the decarbonization of refineries is complex due to the diverse range of processes involved, high energy requirements, and the limited availability of cost-effective clean alternatives. In this study, a holistic approach is proposed for refinery decarbonization based on atomic, energy, and exergy flow analysis by characterizing feedstocks and products using their stoichiometric formulas (CHjOkNmSn) and thermodynamic properties. Applied to the Blueprint model, representing a typical European refinery, it is revealed that 2.4% and 1.3% of carbon emissions stem from the exit of oxygen atoms during combustion and hydrogen production, accounting for 4.3% of total energy input. Decarbonization options for future refineries such as product portfolio changes and alternative feedstocks were further explored. While maximizing naphtha for chemicals leads to increased CO2 emissions due to higher hydrogen-to-carbon ratios in products, biomass with electrolysis emerges as a preferable option for its low carbon losses and high exergy efficiency. The thermodynamic analysis of this study can provide valuable insights and theoretical instructions to industrial operators into the transition to future refineries.

Energy budgeting and global warming potential of traditional rice production system in Eastern Ghats region of Odisha

Aim: This study aims to focus on traditional agricultural practices which are gaining momentum around the globe as an energy efficient and sustainable approach in the changing climate condition. In this study, an attempt was under taken to quantify the energy use, green house gas emission and global warming potential of rice cultivated by traditional method in terraced low land. Methodology: The data were collected through Focused Group Discussion (FGD) from farmers of the study area using the questionnaire. The sample size was calculated by Neyman method. Results: Total input and output energy required for traditional rice production were 8513.0 MJ ha-1 and 77356.88 MJ ha-1, respectively. Energy use efficiency was calculated as 9.01. Total global warming potential (GWP) was 610.19 CO2 eq ha-1. In this study, the output and input carbon were found to be 2580.48 and 164.75 kg C ha-1, respectively and the carbon efficiency ratio was 15.66. Energy productivity of this traditional method of rice production system was approximately 1.21 to 3.64 times higher than that of other rice production system. Interpretation: Rice is the staple food of tribal's habited in the highland region of Eastern Ghats Odisha, India, and these tribal people mostly cultivate rice by traditional method. This traditional rice production system can be considered as an environmental friendly and sustainable agricultural system in this changing climatic condition, and this system is also economical than the conventional rice cultivation practices. Key words: Budgeting, Energy, Eastern Ghats, Green house gases, Global warming, Rice

Abstract 2943: Investigation of microsatellite instability in RNA compared to DNA, using microsatellite targeted, anchored multiplex PCR and NGS

Abstract MSI measurements are typically made on DNA, but it would be useful to make similar measurements from RNA or total nucleic acid inputs (TNA), so that MSI determinations could be made in parallel to interrogation of the RNA. Doing so requires characterizations of microsatellite lengths from those which are transcribed and untranscribed, because both will be detected following cDNA creation. Microsatellite instability (MSI) occurs when small unit repeats of DNA undergo incorrect replication resulting in the growth or contraction of the number of repeats in the newly synthesized DNA. This accumulation of errors at microsatellite DNA is usually mitigated by the mismatch repair (MMR) pathway which can correct these errors. MSI is of interest because it serves as a phenotypic readout of the status of the MMR machinery, and MSI status can correlate to tumor progression and response to agents that regulate these cellular mechanisms. We investigated the status of microsatellites using Anchored Multiplex PCR (AMP™) targeted panels (RUO) and compared results from TNA inputs using an AMP MSI module and both VARIANTPlex™ chemistry to interrogate DNA, and FUSIONPlex™ chemistry to interrogate both RNA + DNA. Our results indicate differences in the diversity of lengths of microsatellites present in RNA, and the resulting stability calls which would be made at those sites. Microsatellite length diversity was highly dependent on the genomic context of the microsatellite. Microsatellites located within transcripts showed increased length diversity when examining the RNA + DNA results compared to DNA alone. Microsatellites located in intergenic regions, which are unlikely to be transcribed, showed no increase in diversity of lengths when comparing the RNA + DNA results to DNA alone. While these results suggest that MSI determinations could be made from RNA or RNA + DNA NGS data, the unique behavior of microsatellites in RNA is likely to require additional investigations and unique data analysis techniques to correctly categorize microsatellites as stable or unstable. Confidential - Company Proprietary Citation Format: Ryan Rogge, Taylor Patterson, Alicia Navetta, Dhruti Legare. Investigation of microsatellite instability in RNA compared to DNA, using microsatellite targeted, anchored multiplex PCR and NGS [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 2943.

A numerical investigation of the thermal characteristics of supercritical carbon dioxide (sCO2) flowing through mini serpentine channels

In this study, we numerically investigate the heat transfer characteristics of CO2 during phase transitions in its supercritical state in serpentine channels. Four different inclination angles are applied to three different radii of curvature flow channels with the same diameter of 2 mm. The supercritical state is maintained by assuming that the mass flux is approximately 191 kg/m2s, the inlet temperature is 298.15 K, and the operating pressure is 7.65 MPa. To ensure a consistent total heat input, different heat flux values are applied to the three different cases, eliminating the potential impact of the increased surface area on the heat transfer performance and allowing a clearer investigation of the geometric effects within the same temperature range. In a channel with a constant radius of curvature, the centrifugal force has a dominant influence on the heat flow compared to gravitational buoyancy, showing a flow phenomenon independent of the angle. In contrast, in a flow channel with a varying radius of curvature, the gravitational buoyancy fluctuates depending on the local area. As a result, the ratio of the centrifugal force to the gravitational buoyancy decreases, causing more variations with changes in the inclination angle. As the centrifugal force contributes significantly to turbulent mixing, the average heat transfer coefficient is highest in the flow channel with the shortest wavelength. As the wavelength increases, the ratio of centrifugal force to buoyancy begins to affect density stratification, especially in gas-like regions, leading to buoyancy. The local heat transfer coefficient at points around the circumference of the cross-section affected by buoyancy and in the film is analyzed, which is closely related to the centrifugal buoyancy and gravitational buoyancy, and their ratio. To express this influence in a correlation equation, a new Nu correlation with an error of less than 25 % is proposed using De, which combines the centripetal force and Reynolds number, ratio of centrifugal buoyancy and gravitational buoyancy, Ф, and Pr as.Nu=0.849De0.541∅-0.154Pr-0.160