Modification Of Topography Research Articles

Development of micropatterning polyimide films for enhanced antifouling and antibacterial properties

A commercial biomedical Polyimide (PI) film was topographically and chemically modified by generating micrometric periodic arrays of lines using Direct Laser Interference Patterning (DLIP) in order to improve antifouling and antibacterial properties. DLIP patterning was performed with periods from 1 μm to 10 μm. The physical modification of the surface was characterized by SEM, AFM and contact angle measurements and, the chemical composition of the ablated surfaces was analyzed by ATR-IR and XPS spectroscopies. The antibacterial effects were evaluated through the effect on Pseudomonas aeruginosa colonies growth on the LB (Luria Bertani) broth. The results showed that the laser treatment change the topography and as a consequence the chemistry surface, also that the microstructured surfaces with periods below 2 μm, exhibited a significant bacterial (P. aeruginosa) adhesion decrease compared with non-structured surfaces or with surfaces with periods higher than 2 μm. The results suggest that periodic topography only confer antifouling properties and reduction of the biofilm formation when the microstructure presents periods ranging from 1 μm to 2 μm. On the other hand, the topography that confer strong antifouling superficial properties persists at long incubation times. In that way, polymer applications in the biosciences field can be improved by a surface topography modification using a simple, single-step laser-assisted ablation method.

Quantification of dental implant surface wear and topographical modification generated during insertion

Titanium dental implants are subjected to frictional stresses during insertion, which may lead to surface wear and modification of original surface topography. The main goal of this study was to comprehensively quantify the wear and topographical modification of dental implants suffered during insertion procedure. Two commercially available dental implant systems with differing topographies: acid etched (AE) and sandblasted+acid etched (SB) were analysed as real case studies. Different regions of the implants (neck, valley, top, flank) were monitored qualitative and quantitatively both before and after insertion in fresh cow rib bone. Wear and plastic deformation mechanisms were identified at the surfaces of the post-inserted implants. The wear mass loss was approximated by the functional volume parameter (Vm) variation analysis resulting in a total average of 36.5 and 1 μg of released particles for the AE and SB implants respectively. The developed interfacial ratio (Sdr) variation correlated positively with the material volume variation (Vm) and is considered the best damage indicator. This study quantified, for the first time, the wear and topographical modifications generated at different sites of dental implants, and illustrates the need to analyse different regions to correctly understand and quantify the effects of the insertion process on dental implant surfaces.

Effect of wavelengths on the structure, morphology and optoelectronic properties of cadmium sulfide thin films by laser assisted chemical bath deposition

Laser assisted chemical bath deposition (LACBD) is an interesting technique to synthesize semiconductor thin films. The effects of three laser wavelengths (445, 457 and 532 nm) on the structure, composition, morphology and optoelectronic properties of cadmium sulfide (CdS) thin films by LACBD are presented. Structural analysis of the thin films confirmed their polycrystalline nature. The surface composition specifics and chemical states of Cd and S were studied by X-ray photoelectron spectra. The topography and roughness of the films were explored by atomic force microscopy. The films exhibited high transmittance in visible region with 2.5 eV bandgap. All the samples showed photocurrent response under irradiation for white light as well as selected wavelengths of visible light emitting diodes from which the electrical properties were evaluated. The results showed that the laser irradiation by various wavelengths was effective in modification of surface topography and optoelectronic properties of the polycrystalline CdS thin films.

Mussel inspired ZIF8 microcarriers: a new approach for large-scale production of stem cells.

Metal–organic frameworks (MOFs) have high porosity, large surface area, and tunable functionality and have been widely used for drug loading. The aim of this study was to exploit unique features of zeolitic imidazolate framework-8 (ZIF8) to develop an innovative composite microcarrier (MC) for human mesenchymal stem cells (hMSCs) adhesion and proliferation. ZIF8 MCs were prepared by immobilization of polydopamine/polyethyleneimine (PDA/PEI) and ZIF8 on the surface of polystyrene beads. The chemical properties of MCs such as coating stability and homogeneity were characterized by different techniques such as ATR-FTIR, XRD, EDX, SEM, and contact angle. The prepared MCs were tested using human adipose-derived mesenchymal stem cells (hADSCs) in both static and dynamic conditions, and results were compared to a commercially available MC (Star-Plus), polydopamine coated MCs and amine-functionalized MCs as a control. Results show that PDA/PEI/ZIF8 coated MCs (in short: ZIF8 MCs) provides an excellent biocompatible environment for hADSCs adhesion and growth. In conclusion, ZIF8 MCs represent suitable and low-cost support for hADSCs culture and expansion, which can maximize cell yield and viability while preserving hADSCs multipotency. The present findings have revealed this strategy has the potential for chemical and topographical modification of MCs in tissue engineering applications.

Compact pulsed thulium-doped fiber laser for topographical patterning of hydrogels

We report the generation of high energy 2 μm picosecond pulses from a thulium-doped fiber master oscillator power amplifier system. The all-fiber configuration was realized by a flexible large-mode area photonic crystal fiber (LMA-PCF). The amplifier output is a linearly-polarized 1.5 ns, 100 kHz pulse train with a pulse energy of up to 250 μJ. Pulse compression was achieved with (2+2)-pass chirped volume Bragg grating (CVBG) to obtain a 2.8 ps pulse width with a total pulse energy of 46 μJ. The overall system compactness was enabled by the all-fiber amplifier design and the multi-pass CVBG-based compressor. The laser output was then used to demonstrate high-speed direct-writing capability on a temperature-sensitive biomaterial to change its topography (i.e. fabricate microchannels, foams and pores). The topographical modifications of biomaterials are known to influence cell behavior and fate which is potentially useful in many cell and tissue engineering applications.

Structure and Topography Modifications of Treated AISI 316LN Stainless Steel Surfaces after Friction in Dry Sliding Contact by Case Hardening Process

Austenitic stainless is having corrosion resistance property, but certain mechanical applications require improved resistance to wear, inferior cavitation, susceptibility to sensitization. These steels have numerous favourable circumstances for great cryogenic - properties, anti-corrosion, and bio-compatibility. So these steels have a broad application in low temperature innovation, saltwater applications, nourishment industry, bio-medicine, petro-chemical handling, and so forth when alloyed with nitrogen, austenitic treated steels has a progressively steady austenite structure, better mechanical properties and better wear opposition, which has animated extraordinary enthusiasm for this exploration work. Many surface hardening techniques are available, the best surface modification technique is chosen for improved service performance. Surface engineering is a technique to alter the surface of a material by mechanical or microchemical method without affecting the material properties. The alterations are done on the surfaces subjected to the liquid nitriding process to produce a hardened surface. Chosen for this research workbased on their wide application, the wear behaviour of AISI 316LN stainless steels was investigated. Of the various surface hardening techniques available, nitriding is chosen, so that these nitrogen gets penetrated into the material, in which hard iron chromium nitrides are formed at the surface level. AISI 316LN specimens were subjected to salt bath nitriding process. The specimens were nitrided to 60 minutes, 120 minutes and 180 minutes respectively. The specimens were undergone with wear tests by standardized tribiological wear machine and finally the metallographic studies were made.

Modification of Surface Topography and Analysis of Its Impact on Friction and Wear Reduction of Sliding Contact

Proper lubrication and surface modification are key factors to improve the tribological behavior of interacting sliding surfaces under lubricated conditions. Surface texturing of interacting surfaces has found to be an emerging technique that modifies the surfaces deterministically by producing surface features in the form of surface asperities or grooves with specific shape, size and distribution. The present paper address the impact of positive surface textures (protrusions) and number of positive textures in the sliding direction on friction and wear behavior of parallel sliding contacts. The square shaped positive surface textures are created on the specimen by ink-jet followed by chemical etching process. The sliding experiments are conducted on pin on disc friction and wear test rig by providing different sliding conditions such as plain dry, plain with lubricant and textures with lubricant between the interacting surfaces. The results indicated that the textures with lubricated condition exhibit lower friction and wear compared to other two conditions. Furthermore, it is reported that among the tested samples, the textured sample with number of textures three in sliding direction has shown a prominent effect in reducing friction and wear of parallel sliding contact.

Heparinized thin-film composite membranes with sub-micron ridge structure for efficient hemodialysis

Hemodialysis membranes are critical components for hemodialysis process. Herein, we demonstrate a new kind of hemodialysis membrane based on polyacrylonitrile (PAN) electrospun scaffold and heparin-poly(vinyl alcohol) (heparin-PVA) hydrogel coating. Manipulation of coating solution rheology by control of heparin content enabled the creation of membranes with sub-micron ridged surface topography. The results of attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectra confirmed that heparin was covalently incorporated in the hydrogel layer; meanwhile, the scanning electron microscopy (SEM) and atomic force microscopy (AFM) images gave clear evidence that the fabricated membranes had typical sub-micron ridge structure. The results revealed that the sub-micron ridged surface topography and heparin chemical modification synergistically endowed the membranes with excellent permeability, protein antifouling properties, anticoagulant activity, cytocompatibility, and dialysis performances. Especially, when the heparin/PVA weight ratio was 20%, the water flux of the membrane was 371.8 L/m2h. Its urea clearance was 86 mL/min, lysozyme clearance was 60 mL/min, and bovine serum albumin clearance was as less as 5.5 mL/min.

Tunable and Magnetic Thiol-ene Micropillar Arrays.

Tunable and responsive surfaces offer routes to multiple functionalities ranging from superhydrophobic surfaces to controlled adhesion. Inspired by cilia structure in the respiratory pathway, magnetically responsive periodic arrays of flexible and magnetic thiol-ene micropillars are fabricated. Omnidirectional collective bending of the pillar array in magnetic field is shown. Local non-contact actuation of a single pillar is achieved using an electromagnetic needle to probe the responsiveness and the elastic properties of the pillars by comparing the effect of thiol-ene crosslinking density to pillar bending. The suitable thiol-ene components for flexible and stiff magnetic micropillars and the workable range of thiol-to-allyl ratio are identified. The wettability of the magnetic pillars can be tailored by chemical and topography modification of the pillar surface. Low-surface-energy self-assembled monolayers are grafted by UV-assisted surface activation, which is also used for surface topography modification by covalent bonding of micro- and nanoparticles to the pillar surface. The modified thiol-ene micopillars are resistant to capillarity-driven collapse and they exhibit low contact angle hysteresis, allowing water droplet motion driven by repeated bending and recovery of the magnetic pillars in an external magnetic field. Transport of polyethylene microspheres is also demonstrated.

Silver Nanoparticles Addition in Poly(Methyl Methacrylate) Dental Matrix: Topographic and Antimycotic Studies.

The widespread use of nanoparticles (NPs) in medical devices has opened a new scenario in the treatment and prevention of many diseases and infections owing to unique physico-chemical properties of NPs. In this way, silver nanoparticles (AgNPs) are known to have a strong antimicrobial activity, even at low concentrations, due to their ability to selectively destroy cellular membranes. In particular, in the field of dental medicine, the use of AgNPs in different kinds of dental prosthesis matrixes could be a fundamental tool in immunodepressed patients that suffer of different oral infections. Candida albicans (C. albicans), an opportunistic pathogenic yeast with high colonization ability, is one of the causative agents of oral cavity infection. In our work, we added monodispersed citrate-capping AgNPs with a size of 20 nm at two concentrations (3 wt% and 3.5 wt%) in poly(methyl methacrylate) (PMMA), the common resin used to develop dental prostheses. After AgNPs characterization, we evaluated the topographical modification of PMMA and PMMA with the addition of AgNPs by means of atomic force microscopy (AFM), showing the reduction of surface roughness. The C. albicans colonization on PMMA surfaces was assessed by the Miles and Misra technique as well as by scanning electron microscopy (SEM) at 24 h and 48 h with encouraging results on the reduction of yeast viability after AgNPs exposure.

Historical museum collections clarify the evolutionary history of cryptic species radiation in the world's largest amphibians.

Inaccurate taxonomic assessment of threatened populations can hinder conservation prioritization and management, with human‐mediated population movements obscuring biogeographic patterns and confounding reconstructions of evolutionary history. Giant salamanders were formerly distributed widely across China, and are interpreted as a single species, Andrias davidianus. Previous phylogenetic studies have identified distinct Chinese giant salamander lineages but were unable to associate these consistently with different landscapes, probably because population structure has been modified by human‐mediated translocations for recent commercial farming. We investigated the evolutionary history and relationships of allopatric Chinese giant salamander populations with Next‐Generation Sequencing methods, using historical museum specimens and late 20th‐century samples, and retrieved partial or near‐complete mitogenomes for 17 individuals. Samples from populations unlikely to have been affected by translocations form three clades from separate regions of China, spatially congruent with isolation by either major river drainages or mountain ranges. Pliocene–Pleistocene divergences for these clades are consistent with topographic modification of southern China associated with uplift of the Qinghai‐Tibet Plateau. General Mixed Yule Coalescent model analysis indicates that these clades represent separate species: Andrias davidianus (Blanchard, 1871) (northern Yangtze/Sichuan), Andrias sligoi (Boulenger, 1924) (Pearl/Nanling), and an undescribed species (Huangshan). Andrias sligoi is possibly the world's largest amphibian. Inclusion of additional reportedly wild samples from areas of known giant salamander exploitation and movement leads to increasing loss of biogeographic signal. Wild Chinese giant salamander populations are now critically depleted or extirpated, and conservation actions should be updated to recognize the existence of multiple species.

New immobilization method of anti-PepD monoclonal antibodies for the detection of Listeria monocytogenes p60 protein – Part A: Optimization of a crosslinked film support based on chitosan and cellulose nanocrystals (CNC)

This paper presents the development of a support membrane based on chitosan, cellulose nanocrystals and glycerol (m-CCG) for the antibody immobilization by a covalent crosslinking using glutaraldehyde. The chemical characterization of the support by FTIR showed that m-CCG formation process was stabilized by the formation of hydrogen bonding between each component of m-CCG and the reactive amine groups allowing the antibody immobilization on m-CCG via glutaraldehyde. Moreover, this immobilization on m-CCG was optimized by mathematics modeling approaches, and it exhibited robustness and predictable detection in presence of 0.6% of cellulose nanocrystals (CNCs), 0.5 g of CCG solution per well, after 2 h of antibody immobilization. Results also showed that CNCs (0.6% w/v) was the most important factor of the optimization. At this concentration, CNCs improve the resistance of m-CCG during the crosslinking treatment by a modification of the surface topography and the reinforcement of the tensile strength of m-CCG at >30%.

Tuning the biomimetic behavior of scaffolds for regenerative medicine through surface modifications.

Tissue engineering and regenerative medicine rely extensively on biomaterial scaffolds to support cell adhesion, proliferation, and differentiation physically and chemically in vitro and in vivo. Changes to the surface characteristics of the scaffolds have the greatest impact on cell response. Here, we discuss five dominant surface modification approaches used to biomimetically improve the most common scaffolds for tissue engineering, those based on aliphatic polyesters. Scaffolds of aliphatic polyesters such as poly(l-lactic acid), poly(l-lactic-co-glycolic acid), and poly(ε-caprolactone) are often used in tissue engineering because they provide desirable, tunable properties such as ease of manufacturing, good mechanical properties, and nontoxic degradation products. However, cell-surface interactions necessary for tissue engineering are limited on these materials by their smooth postfabrication surfaces, hydrophobicity, and lack of recognizable biochemical binding sites. The surface modification techniques that have been developed for synthetic polymer scaffolds reduce initial barriers to cell adhesion, proliferation, and differentiation. Topographical modification, protein adsorption, mineral coating, functional group incorporation, and biomacromolecule immobilization each contribute through varying mechanisms to improving cell interactions with aliphatic polyester scaffolds. Furthermore, rational combination of methods from these categories can provide nuanced, specific environments for targeted tissue development.

The influence of high-dose systemic zoledronate administration on osseointegration of implants with different surface topography.

To evaluate the influence of systemic zoledronate administration on the osseointegration of titanium implants with different surface topography in rat maxillae. Twenty Sprague-Dawley rats were divided into two groups-test (bisphosphonate) and control (healthy). Bisphosphonate administration began three weeks prior to implant placement, and the animals received zoledronate (66μg/kg) three times per week. Forty endosseous implants with a moderately rough (20 implants) or a turned surface (20 implants) were immediately placed bilaterally into extraction sockets of maxillary first molars. Animals were sacrificed after 14 and 28days of healing, and en bloc specimens were harvested for histological and histomorphometric analysis. Osseointegration was quantified by measuring the percentage of bone-to-implant contact. Bone-to-implant contact (BIC) (mean±SD) values of moderately rough and turned implants at day 14 in test group were 17.62±6.68 and 10.69±1.48, respectively, while in the control group, they were 46.36±5.08 and 33.29±8.89, respectively. At day 28, BIC values of moderately rough and turned implants in the test group were 25.94±7.87 and 7.83±4.30, respectively, while in the control group, they were 72.99±6.60 and 47.62±18.19, respectively. Statistically significant higher BIC values were measured on moderately rough implants compared to turned implants at 28days, and the control group compared to the test group for both implant surfaces. Histological observations for the control and the test groups demonstrated initial bone formation around moderately rough implants not only on the surface of the parent bone, as was the case with the turned surfaced implants, but also along the implant surface itself. Systemic zoledronate administration negatively influences osseointegration. Osseointegration was enhanced adjacent to moderately rough compared to turned implants in both the presence and absence of systemic zoledronate administration. Therefore, topographical surface modification may partially offset the negative impact of zoledronate administration.

Amplification and regulation of periodic nanostructures in multipulse ultrashort laser-induced surface evolution by electromagnetic-hydrodynamic simulations

The formation of periodic structures in ultrafast laser-irradiated surfaces implies dynamic coupling between the incoming light and the light-driven material. To capture the mutual influence and feedback between light and evolving surface topographies, we investigate numerically the evolution of metal surfaces irradiated by multiple femtosecond laser pulses of sub-, near-, and slightly above-threshold ablation fluence. The multiphysical model combines Maxwell equations and thermohydrodynamic approach based on electron-ion heat transfer and compressible Navier-Stokes equations and allows us to account for interpulse feedback on the resulting surface topographies. First pulses of the subthreshold energy lead to material swelling, nanocavitation few tens of nanometers below the surface, and, as a result, nanoroughness formation on the initially flat surface. Further pulses contribute to the development of periodic surface structures, enhanced absorption, and increased removal rate. Cavitation in the tips of ripples is found to play a crucial role in modification and regulation of surface topography for sub- and near-threshold ablation fluences. At higher laser pulse energy, thermal ablation is mostly involved in surface modification, and the ablation rate per power reaches its maximum at three to five times the ablation threshold fluence, resulting from the optimal heat penetration depth for laser ablation. The numerical results offer a better understanding of the surface topography modifications upon multipulse femtosecond laser irradiation.

Titanium with nanotopography induces osteoblast differentiation through regulation of integrin αV.

Topographical modifications of titanium (Ti) at the nanoscale level generate surfaces that regulate several signaling pathways and cellular functions, which may affect the process of osseointegration. Here, we investigated the participation of integrin αV in the osteogenic capacity of Ti with nanotopography. Machined titanium discs (untreated) were submitted to treatment with H2 SO4 /H2 O2 to produce the nanotopography (nanostructured). First, the greater osteogenic capacity of the nanotopography that increased osteoblast differentiation of mesenchymal stem cells compared with untreated topography was shown. Also, the nanostructured surface increased (regulation ≥ 1.9-fold) the gene expression of 6 integrins from a custom array plate utilized to evaluate the gene expression of 84 genes correlated with cell adhesion signaling pathway, including integrin αV, which is involved in osteoblast differentiation. By silencing integrin αV in MC3T3-E1 cells cultured on nanotopography, the impairment of osteoblast differentiation induced by this surface was observed. In conclusion, it was shown that nanotopography regulates the expression of several components of the cell adhesion signaling pathway and its higher osteogenic potential is, at least in part, due to its ability to upregulate the expression of integrin αV. Together with previous data that showed the participation of integrins α1, β1, and β3 in the nanotopography osseoinduction activity, we have uncovered the pivotal role of this family of membrane receptors in the osteogenic potential of this surface.

Assessment of surface topography modifications through feature-based registration of areal topography data

Surface topography modifications due to wear or other factors are usually investigated by visual and microscopic inspection, and—when quantitative assessment is required—through the computation of surface texture parameters. However, the current state-of-the-art areal topography measuring instruments produce detailed, areal reconstructions of surface topography which, in principle, may allow accurate comparison of the individual topographic formations before and after the modification event. The main obstacle to such an approach is registration, i.e. being able to accurately relocate the two topography datasets (measured before and after modification) in the same coordinate system. The challenge is related to the measurements being performed in independent coordinate systems, and on a surface which, having undergone modifications, may not feature easily-identifiable landmarks suitable for alignment. In this work, an algorithmic registration solution is proposed, based on the automated identification and alignment of matching topographic features. A shape descriptor (adapted from the scale invariant feature transform) is used to identify landmarks. Pairs of matching landmarks are identified by similarity of shape descriptor values. Registration is implemented by resolving the absolute orientation problem to align matched landmarks. The registration method is validated and discussed through application to simulated and real topographies selected as test cases.

An Active Ease-Off Topography Modification Approach for Hypoid Pinions Based on a Modified Error Sensitivity Analysis Method

A new active ease-off topography modification approach is proposed to improve the meshing performance of hypoid gears based on a fourth-order predesigned transmission error (PTE) model and a modified error sensitivity analysis method. Ease-off topography modifications that describe local deviations of pinion tooth surfaces can be conducted by converting the fourth-order PTE into equivalent deviations of pinion tooth surfaces. The modified error sensitivity analysis method is developed to investigate the effects of misalignments on the moving velocity of a contact point of a hypoid gear pair. The moving velocity of the contact point can describe transmission error (TE) curve shapes of ease-off tooth surfaces. The ease-off topography modification approach can achieve TE precontrol and modification curvature adjustment of the pinion for stable meshing performance of the hypoid gear pair. Moreover, pinion ease-off tooth surfaces are finished by a five-axis computer numerical control swarf-cutting machine tool. Swarf-cutting tests and TE measurement tests are conducted on hypoid gear pair specimens to demonstrate the feasibility and effectiveness of the proposed methodology.

Enhanced corrosion resistance of zinc-containing nanowires-modified titanium surface under exposure to oxidizing microenvironment

Titanium (Ti) and its alloys as bio-implants have excellent biocompatibilities and osteogenic properties after modification of chemical composition and topography via various methods. The corrosion resistance of these modified materials is of great importance for changing oral system, while few researches have reported this point. Recently, oxidative corrosion induced by cellular metabolites has been well concerned. In this study, we explored the corrosion behaviors of four common materials (commercially pure Ti, cp-Ti; Sandblasting and acid etching-modified Ti, Ti-SLA; nanowires-modified Ti, Ti-NW; and zinc-containing nanowires-modified Ti, Ti-NW-Zn) with excellent biocompatibilities and osteogenic capacities under the macrophages induced-oxidizing microenvironment. The results showed that the materials immersed into a high oxidizing environment were more vulnerable to corrode. Meanwhile, different surfaces also showed various corrosion susceptibilities under oxidizing condition. Samples embed with zinc element exhibited more excellent corrosion resistance compared with other three surfaces exposure to excessive H2O2. Besides, we found that zinc-decorated Ti surfaces inhibited the adhesion and proliferation of macrophages on its surface and induced the M2 states of macrophages to better healing and tissue reconstruction. Most importantly, zinc-decorated Ti surfaces markedly increased the expressions of antioxidant enzyme relative genes in macrophages. It improved the oxidation microenvironment around the materials and further protected their properties. In summary, our results demonstrated that Ti-NW-Zn surfaces not only provided excellent corrosion resistance properties, but also inhibited the adhesion of macrophages. These aspects were necessary for maintaining osseointegration capacity and enhancing the corrosion resistance of Ti in numerous medical applications, particularly in dentistry.

Effect of surface integrity induced by machining on high cycle fatigue life of 7075-T6 aluminum alloy

The metallurgical, mechanical and topographical modifications induced by machining strongly influence the surface quality of the final components which, in turn, dictates the functional performance and service-life of engineered products. This paper aims to extensively discuss the effect of machining parameters and cooling/lubrication strategies on the high cycle fatigue strength of 7075-T6 aluminum alloy. More in detail, three different cutting speeds (90, 120 and 150 m/min) and four cooling/lubrication methodologies (dry, cryogenic, minimum quantity lubrication and high pressure air jet) have been analyzed. The obtained fatigue samples have been tested under fully reversed stress conditions in the high cycle fatigue regime. An adapted analytical model, taking into account the effect of the surface changes, is also proposed in order to drastically reduce the number of destructive tests needed assessing the effects of the processing parameters on the overall fatigue endurance.