Topical Platelet-rich Plasma Research Articles

Role of Masson’s trichrome stain in evaluating the effect of platelet-rich plasma on collagenesis after induction of thermal burn in adult male albino rats

Background Burns are a global health problem, accounting for about 180 000 deaths annually especially in low- and middle-income countries. Many reports have concentrated on the role of platelet-rich plasma (PRP) in the treatment of soft tissue burn lesions. Aim This work aimed to study the role of Masson’s trichrome stain in evaluating the effect of PRP on induced thermal burn in adult male albino rats. Materials and methods In all, 50 adult male albino rats were divided into blood donor group (10 rats), control group (10 rats), and experimental group (30 rats). The experimental group was further subdivided into subgroups A, B, and C. For the experimental group, the rats were anesthetized and exposed to thermal burn. Subgroup A were subjected to contact thermal burn and left without treatment. Subgroup B were subcutaneously injected with 0.3 ml of autologous PRP. In subgroup C autologous topical PRP was applied. Skin specimens were obtained and stained by Masson’s trichrome for histological evaluation at days 1, 7, 14, and 28 after burn induction. Results The subgroups that were treated by PRP were found with better collagen organization and earlier restoration of normal histological features of skin tissue. Conclusion Application of different forms of PRP (injectable and topical) helps healing of burn wounds.

A study to assess the efficacy of fractional carbon dioxide laser with topical platelet-rich plasma in the treatment of androgenetic alopecia.

Owing to the inadequate response to and limitations of the approved medications for androgenetic alopecia, novel therapies are warranted to enhance outcomes. The objective of this paper is to ascertain the effectiveness of fractional carbon dioxide laser followed by platelet-rich plasma application on hair regrowth and to compare the two laser settings. Seven participants were enrolled in this half-head pilot study based on different laser pulse energy and density values (12 mJ, 800 spots/cm2 and 22 mJ, 400 spots/cm2). Ten treatment sessions were performed every 2 weeks with 12-week follow-up. The evaluation methods were based on hair density from standardized phototrichograms, global photographic assessment, and patient satisfaction. Significantly increased total and terminal hair densities were observed during treatment in both groups as compared with baselines. The mean total hair density has weekly increased significantly with high pulse energy (1.42 hair/cm2) as compared to low pulse energy (1.04 hair/cm2) throughout the study period (p = 0.023; 4.89 hair/cm2). Global photographs and patient satisfaction scores demonstrated improvement, but only the latter showed statistical significance (p = 0.029). The average pain intensity scores were not different between the groups (p = 0.760) all over the procedure. The adverse events were minor and well tolerated. This synergistic treatment remarkably leads to hair restoration and high patient satisfaction. The parameters of 22-mJ beam energy with 400 spots/cm2 density are appropriate and superior to 12-mJ beam energy with 800 spots/cm2. Albeit higher energy, no more pain is indicated in the high pulse energy group. Trial registration number (TRN): TCTR20180619004, Thai Clinical Trials Registry (TCTR) since 2018-05-15 13:39:48.

Proficiency of topical platelet-rich plasma with vacuum-assisted closure over platelet-rich plasma alone in diabetic foot ulcers - A clinical prospective comparative study

Proficiency of topical platelet-rich plasma with vacuum-assisted closure over platelet-rich plasma alone in diabetic foot ulcers - A clinical prospective comparative study

Micro needling vs micro needling combined with autologous topical platelet rich plasma in the treatment of melasma: a prospective randomized comparative study

<p><strong>Background: </strong>Melasma is one of the most common pigmentary disorders worldwide with a still unresolved pathogenesis and treatment continues to be challenging. To assess the effect of micro-needling vs combination of micro-needling followed by application of topical platelet rich plasma (PRP) in the treatment of melasma.</p><p><strong>Methods: </strong>Sixty patients having melasma were randomly grouped into A and B. Group A underwent micro-needling alone and group B was subjected to micro-needling followed by topical application of autologous PRP. The patients were subjected to 3 treatment sessions at monthly intervals. Melasma area and severity index (MASI) and patient satisfaction scores were recorded at each sitting and the final outcome was recorded one month after the last session.</p><p><strong>Results: </strong>Twenty-four patients in group A and 27 patients from group B were selected for final analysis. There was significant improvement of MASI in both groups (Group A: p=0.001, group B: p=0.0001) however, the difference in improvement of MASI between the 2 groups was not significant (p=0.0457) Group B was highly satisfied with the treatment which was statistically significant (p=0.0001)</p><p><strong>Conclusions: </strong>Combining micro-needling with topical PRP appears to be a promising therapeutic modality in the treatment of melasma.</p><p><strong> </strong></p>

Effect of topical application of platelet-rich plasma on chronic venous leg ulcerations.

Wound products that reliably support healing of chronic leg ulcers remain a huge unmet need in clinical practice. Due to the lack of standardized comparable protocols and different systems for platelet-rich plasma (PRP) preparation, there is limited data on healing rates in chronic venous ulcers. In our case series with a total of seven chronic leg ulcers in four patients, we investigated the healing rates based on standardized digital photographs of chronic venous ulcers after application of topical PRP using a digital imaging software. In 5 out of 7 ulcers, the PRP-treated wound half showed faster healing as compared the control half of the wound. In this case series, PRP-treated sides of chronic venous leg ulcers showed a tendency for accelerated healing as compared to nontreated collateral wound side. Our data support the evaluation of topical PRP treatment in the management of chronic venous leg ulcers.

Microneedling and Its Use in Hair Loss Disorders: A Systematic Review.

IntroductionMicroneedling (MN) is a minimally invasive procedure involving the induction of percutaneous wounds with medical-grade needles. In this literature review, we investigate clinical data on MN for the treatment of hair loss disorders.MethodsA literature search was conducted through PubMed up to November 2021 to identify original articles evaluating the use of MN on hair loss disorders. The database was searched using the following keywords: “microneedling,” “micro needling,” “micro needle,” “microneedle,” “needle,” “dermaroller” and “alopecia,” “hair loss,” “alopecia,” “areata,” “cicatricial,” or “effluvium.”ResultsA total of 22 clinical studies featuring 1127 subjects met our criteria for inclusion. Jadad scores ranged from 1 to 3, with a mean of 2. As an adjunct therapy, MN improved hair parameters across genders and a range of hair loss types, severities, needling devices, needling depths of 0.50–2.50 mm, and session frequencies from once weekly to monthly. Across 17 investigations totaling 911 androgenic alopecia (AGA) subjects, MN improved hair parameters when paired with 5% minoxidil, growth factor solutions, and/or platelet-rich plasma (PRP) topicals, or when introduced to subjects whose hair count changes had plateaued for ≥ 6 months on other treatments. Across four investigations on 201 alopecia areata (AA) subjects, MN improved hair parameters as a standalone therapy versus cryotherapy, as an adjunct to 5-aminolevulinic acid and photodynamic therapy, and equivalently when paired with topical PRP versus carbon dioxide laser therapy with topical PRP. Across 657 subjects receiving MN, no serious adverse events were reported.ConclusionsClinical studies demonstrate generally favorable results for MN as an adjunct therapy for AGA and AA. However, data are of relatively low quality. Significant heterogeneity exists across interventions, comparators, and MN procedures. Large-scale randomized controlled trials are recommended to discern the effects of MN as a standalone and adjunct therapy, determine best practices, and establish long-term safety.Supplementary InformationThe online version contains supplementary material available at 10.1007/s13555-021-00653-2.

The Effect of Platelet Rich Plasma on Tissue Healing after Mandibular Third Molar Surgery

Introduction: This study aimed, effect of platelet-rich plasma (PRP) on soft tissue healing after mandibular third molar surgery. Materials & Methods: In this semi-blinded clinical trial study,30 selected patients requiring surgical extraction of soft tissue impacted mandibular third molar participated from cases referred to the Department of Surgery, Faculty of Dentistry, Isfahan Azad University 2017.Patients divided into both test and control groups. PRP was placed in the extracted socket of the test group, whereas the control group had no PRP. Arzhangian standard kit utilized for preparation of PRP. The outcome variables in this study were pain, swelling, inter incisal mouth opening, wound dehiscence, dry socket, bleeding, and tissue color. The collected data analyzed using statistical tests followed independent T-test, Mann-Whitney, and fisher (p value < 0.05). Results: The mean postoperative pain score (Visual Analog Scale) lowered for the test group after several wound dehiscence on the third and seventh days after surgery which was statistically significant (p value < 0.001). Although the mean bleeding time on the third day after surgery decreased in the test group, this difference was not statistically significant (p value = 0.59). There were no statistically significant between both groups for The dry socket incidence on the seventh day and the mean of inter incisal mouth opening in the third and seventh days after surgery as well. Conclusion: The usage of topical PRP has beneficial advantages on soft tissue healing after mandibular third molar surgery. Unlike the control group, The PRP group provided reduced pain and better soft tissue healing.

Application of Topical Sucralfate and Topical Platelet-Rich Plasma Improves Wound Healing in Diabetic Ulcer Rats Wound Model.

PurposeOne of the most serious and devastating complications of diabetes mellitus is diabetic ulcers. They are difficult to treat and often result in limb loss. Topical sucralfate and platelet-rich plasma have the potential to improve the healing outcomes of chronic ulcers, including diabetic ulcers. This research aims to determine the effectiveness of sucralfate and platelet-rich plasma therapy for the improvement of diabetic ulcer wound healing.Patients and MethodsNinety Wistar rats were used in this study and were classified into five groups. Four of the five groups were diabetic induced and were treated with topical sucralfate only, platelet-rich plasma only, combination of topical sucralfate and platelet-rich plasma, and diabetic control group which received standard therapy only. The non-diabetic control group did not receive any therapy. We observed macrophage amount, platelet-derived growth factor, vascular endothelial growth factor, and hypoxia-inducible factor as a biomarker. Rats were terminated after 7th and 14th days and were subjected to immunohistochemistry staining and examination.ResultsWe found that topical sucralfate and platelet-rich plasma increase macrophage levels, vascular endothelial growth factor expression and platelet-derived growth factor expression in diabetic wound cells. We also found a reduction in hypoxia inducible factor-1α expression. Combination of topical sucralfate and platelet-rich plasma for 14 days gave the most significant improvement in terms of wound healing compared to topical sucralfate or platelet-rich plasma alone.ConclusionThe combination of topical sucralfate and platelet-rich plasma therapy results in the best improvement in diabetic ulcer wound healing compared to sucralfate or platelet-rich plasma monotherapy or conventional wound healing therapy.

Comparison Between Topical Sucralfate, Topical Platelet-Rich Plasma and Topical Ozonated Virgin Coconut Oil to Enhance Wound Healing in Diabetic Ulcer Mice model

Diabetic ulcer is a serious complication of diabetes mellitus which can lead to further morbidity. Sucralfate, Platelet-rich plasma (PRP) and ozonated virgin coconut oil (VCO) has been shown to provide good clinical outcomes in chronic ulcer healing. This study compares the effectiveness of topical sucralfate, topical PRP and topical ozonated VCO therapy for diabetic ulcer treatment in Wistar mice by observing wound length and vascular endothelial growth factor (VEGF). Ninety Wistar mice with full-thickness ulcer model on the back were divided into 5 groups. Four groups of mice were receiving diabetic induction using streptozotocin injection and were allocated as topical sucralfate (TS), topical PRP (TP), and topical ozonated VCO (TO) group. The remaining diabetic-positive mice were allocated as the diabetic-positive control group (PC) which received standard therapy, and the other non-diabetic mice were allocated as the diabetic-negative control group (NC) which did not receive any therapy. Topical ozonated VCO enhances the wound healing process better with the greatest wound reduction area and highest VEGF expression compared to topical sucralfate and topical PRP. Topical ozonated VCO can potentially accelerate wound healing in diabetic ulcers better than topical sucralfate and topical PRP.

Combination of platelet rich plasma and stromal vascular fraction on the level of vascular endothelial growth factor in rat subjects experiencing deep dermal burn injury

BackgroundThe healing process of burns includes coagulation, inflammation, and remodeling. Vascular endothelial growth factor (VEGF) is involved throughout this healing process. Stem cells from the platelet-rich plasma (PRP) with stromal vascular fraction (SVF) can increase concentrations of growth factors, including VEGF. This is expected to accelerate burn healing. The aim of this study was to determine the effect of a combination of PRP and SVF on VEGF levels in a rats model of deep dermal burn wound healing. Materials and methodsThis is an experimental research study in rats using a post-test control group design with 4 groups: A) control, B) Vaseline, C) topical PRP and SVF, and D) PRP and SVF injection. Burn wounds were induced according to the modified Guo method. ResultsIn a rats model of deep dermal wound healing, topical Vaseline significantly increased serum VEGF compared to control. Topical application and injection of stem cells also significantly increased serum VEGF compared to control and Vaseline. The VEGF concentration was significantly higher following injection of PRP and SVF, suggesting that the injection route is more effective at increasing VEGF levels compared to the topical application of stem cells. ConclusionThe combination of PRP and SVF, either by injection or topical application, can increase VEGF levels during the healing process from deep dermal burns.

Fractional erbium: YSGG laser compared to the combination of fractional erbium: YSGG laser and topical autologous platelet-rich plasma for treatment of atrophic acne scars: a case report

Background: Acne scar is the most common complication in acne that occurs in about 49% of acne individuals. Fractional resurfacing lasers such as CO2 laser and erbium-doped yttrium aluminum garnet (Er: YAG) have been widely used for acne scar treatment and give satisfying results have complications such as pain, hyperpigmentation, infection, prolonged erythema, and scarring. Fractional erbium: yttrium scandium gallium garnet (Er: YSGG) laser solves previous laser techniques' disadvantages. It has an ideal wavelength of 2790 nm, which balances the epidermis layer's vaporization and thermal damage in the dermis's superficial layer. Platelet Rich Plasma (PRP) is an autologous concentration of human platelets in a small plasma volume that secreted various growth factors that can speed up the scar healing process.Case report: A-24 years old male student came with a chief complaint of acne scars on his face four years before the examination. The patient's condition is classified as moderate-severe acne scarring based on Goodman and Baron's criteria (qualitative global acne scarring system). Acne scars treatment plan was single fractional erbium: YSGG laser for the left side of the face and combined fractional Er: YSGG laser with topical autologous PRP on the right side. This procedure was performed in 2 sessions with an interval of 4 weeks, the final evaluation at 12 weeks after the first procedure. Clinical improvement of acne scars was assessed using qualitative and quantitative scales based on Goodman and Baron's, patient and observer scar assessment scale (POSAS), quartile grading scale, and patient satisfaction level.Conclusion: In the final evaluation, there was an improvement on the entire grading scale on the right side of the face (Er: YSGG laser + PRP) and the face's left side (Er: YSGG laser only). Post-treatment erythema was found on both sides of the patient's face but disappeared within three days on the face's right side and five days on the face's left side.

ADJUNCTIVE THERAPY FOR FETLOCK FLEXURAL DEFORMITIES ACCOMPANIED BY A DEEP TISSUE INJURY IN A CALF: A CASE REPORT

A five-day-old Holstein Friesian male calf was presented for the evaluation of severe fetlock flexural deformities with a large pressure ulcer over the right metacarpal bone. Superficial and deep digital flexor tenotomy was performed. The surgical wound recovered well, but 15 days after surgery, the skin over the pressure ulcer was starting to fall away and exposing tendon and muscle. A hoof toe-extension shoe was applied to decrease pressure on the wound. The wound had a moderate bacterial infection and slowly responded to cleansing twice a day with 0.9% normal saline, 10% povidone iodine and a topical antibiotic. Topical autologous platelet-rich plasma (PRP) was then applied to the lesion ([Formula: see text][Formula: see text]cm) twice. The wound showed rapid tissue regeneration, good quality wound healing and no complications. After PRP treatment, the lesion size decreased ([Formula: see text][Formula: see text]cm). At this point, 0.9% normal saline cleansing and a bandage were applied until the wound had completely healed. This case report suggests that the hoof toe-extension shoe and topical dressing with autologous PRP, as a combination treatment, could be effective for fetlock flexural deformities with skin defects or delayed wound healing in large animals.

EFFICACY OF FRACTIONAL CO2 LASER WITH TOPICAL PLATELET RICH PLASMA IN TREATMENT OF ACNE SCARS

Background: Acne scars are most distressing sequelae of acne vulgaris having a major impact on psychosocial aspect and quality of life of an individual. In spite of various therapeutic modalities available treatment of acne scars is challenging. Resurfacing techniques such as Fractional Co2 Laser is a well-established treatment option for acne scar. Platelet-Rich Plasma (PRP), an autologous preparation of growth factors helps in wound healing and tissue regeneration.
 Aim: To study the efficacy of Fractional CO2 Laser with Topical Platelet-Rich Plasma in Acne scars.
 Materials and Methods: A total of 15 patients with atrophic facial acne scar, aged 18-40 years attending Outpatient Department of Dermatology, Venereology and Leprosy at tertiary care centre during January 2019 to December 2019 were included for this interventional study after written informed consent. The patients underwent six sessions of Fractional CO2 Laser with Topical application of Platelet-Rich Plasma at monthly intervals. Pre and post treatment comparative Goodman and Baron’s quantitative global scarring grading and patients self-assessment score were used for assessment.
 Results: At the end of study mild reduction in Goodman and Baron’s quantitative score for acne scar was noted in 13 patients (86.6%). Mean Goodman and Baron’s quantitative score att 4 weeks after sixth sitting was 13.13 ± 4.60. On patients self-assessment moderate resolution of acne scar was noted in 8 patients (53.3%).
 Conclusion: Fractional CO2 Laser with Topical Platelet-Rich Plasma application has mild to moderate improvement of acne scars and shorter down time of Fractional CO2 Laser.
 Keywords: Acne scar, Fractional Co2 Laser, Topical Platelet-Rich Plasma

The Role of Platelet Homeostasis in a Novel Topical PRP Formulation.

Topical platelet-rich plasma (PRP) must demonstrate stability to insure biologic activity in aesthetic medicine. The objective of this research was to evaluate the role of platelet homeostasis in a novel PRP topical cosmetic formulation to provide facial appearance improvement. The stability of the topical PRP formulation was evaluated in vitro followed by clinical in vivo testing. The in vitro evaluation examined platelet stability and morphology over a 90-day period within the preservative cosmetic base utilizing ELISA and light microscopy (LM)/scanning electron microscopy (SEM). The in vivo clinical study enrolled 20 subjects in a 120-day double blind split face study to evaluate the effect of 5–7x concentrated PRP compared to 2–3x concentrated PRP on facial photoaging. Cosmetic effect was evaluated by the subject and the dermatologist investigator on a 5-point ordinal scale at baseline, week 8, and week 16. 90-day stability for the topical PRP formulation was verified via ELISA and LM/SEM. ELISA showed the PRP was more inactive than control conditions via analyte concentration curves (PDGF-AB, EGF, and P-Selectin). LM/SEM demonstrated the PRP had less aggregation/activation over time within the cosmetic base and that refrigeration is superior to room-temperature storage thus delaying full platelet degranulation. The in vivo clinical study demonstrated parity between 20ml and 60ml PRP in terms of clinical efficacy. Platelets remain viable for up to 90 days in a refrigerated cosmetic vehicle with demonstrated topical clinical PRP facial benefits. PRP kits of 20ml and 60ml volumes for topical PRP are equally efficacious. J Drugs Dermatol. 2020;19(12): doi:10.36849/JDD.2020.5495.

Autologous Platelet-Rich Plasma Enhances the Healing of Large Cutaneous Wounds in Dogs.

Platelet-rich plasma (PRP) is known to play a crucial role in skin wound healing, in both Human and Veterinary Medicine. Remarkably, until now, no studies have reported PRP treatment in subacute full-thickness skin wounds of the dog. The aim of this study was to evaluate the effects of two consecutive applications of autologous PRP, with the second application after 15 days, in 6 dogs showing large subacute skin wounds. The percentage of contraction, re-epithelialization and healing in all treated patients indicated that no complications or side effects, associated with consecutive PRP treatments, occurred in any patient and all wounds achieved complete closure and re-epithelialization. Our results suggest a positive effect of repeated autologous topical PRP treatments in large cutaneous subacute wounds of different etiology. Therefore, this PRP treatment could represent a simple, cost-effective, and valid alternative to promote healing processes in subacute large wounds cases in dogs.

A Simple Drug Delivery System for Platelet-Derived Bioactive Molecules, to Improve Melanocyte Stimulation in Vitiligo Treatment.

Vitiligo is the most common depigmentation disorder of the skin. Currently, its therapy focuses on the halting of the immune response and stimulation of the regenerative processes, leading to the restoration of normal melanocyte function. Platelet-rich plasma (PRP) represents a safe and cheap regenerative therapy option, as it delivers a wide spectrum of native growth factors, cytokines and other bioactive molecules. The aim of this study was to develop a simple delivery system to prolong the effects of the bioactive molecules released from platelets. The surface of electrospun and centrifugally spun poly-ε-caprolactone (PCL) fibrous scaffolds was functionalized with various concentrations of platelets; the influence of the morphology of the scaffolds and the concentration of the released platelet-derived bioactive molecules on melanocytes, was then assessed. An almost two-fold increase in the amount of the released bioactive molecules was detected on the centrifugally spun vs. electrospun scaffolds, and a sustained 14-day release of the bioactive molecules was demonstrated. A strong concentration-dependent response of melanocyte to the bioactive molecules was observed; higher concentrations of bioactive molecules resulted in improved metabolic activity and proliferation of melanocytes. This simple system improves melanocyte viability, offers on-site preparation and is suitable for prolonged topical PRP administration.

Micro Needling with Platelet Rich Plasma Versus Fractional CO2 Laser with Platelet Rich Plasma in Patients with Post Acne Scars

Background: Facial scaring has always been challenged to treat and there are many treatments available to decrease the appearance of scars like topical retinoids, chemical peeling, microneedling, dermabrasion, soft tissue fillers, botulinum toxoid injection and laser resurfacing The aim of this study was to evaluate and compare the efficacy of microneedling and fractional CO2 laser in the treatment of post acne scars. A comparative studyOf thirty patients (12 males and 18 females) aged 19 – 36 years with atrophic acne scars were devided into Group (A): treated with Fractional Co2 laser combined with topical platelet rich plasma. Group (B): treated with skin microneedling combined with topical platelet rich plasma. Platelet rich plasma can improve the quality of atrophic acne scars treated with ablative fractional CO2 laser or microneedling and decrease the duration of related side effects including oedema and erythema. Regarding patient’s satisfaction grades there was an improvement after both treatment modalities with insignificant differences between both treatment modalities. PRP can improve the quality of atrophic acne scars treated with ablative fractional CO2 laser or microneedling and decrease the duration of laser-related side effects including oedema and erythema.

Evaluation of safety and efficacy of autologous topical platelet gel vs platelet rich plasma injection in the treatment of venous leg ulcers: A randomized case control study.

Various management options were tried for Chronic venous leg ulcers (VLUs) with no satisfactory results. Platelet-rich plasma (PRP) is promising in enhancing chronic wound healing by releasing various growth factors. We evaluated the safety and efficacy of topical platelet gel (PG) vs PRP injection in treatment of venous leg ulcers. 20 patients were treated with topical PG (group I), 20 patients received PRP injection (group II), and 20 patients were treated with saline dressing and compression as controls (group III) weekly for 6 weeks. Ulcer improvement was evaluated by pressure ulcer scale for healing (PUSH). Histopathological evaluation and immunohistochemical staining using anti-CD34 were performed before and after treatment. There was significant improvement in PUSH score in group (I) and (II) compared to group (III; P value: .001). However, there was no statistically significant difference in improvement between group I and II (P value = .83). However, topical PG was more effective in diabetics and PRP was more effective in long standing fibrosed ulcers. Histopathology showed reduction of the inflammatory cells infiltrates and well-formed granulation tissue with no difference in immunostaining. So, both topical PG and PRP injection were effective, low cost, and safe procedures in enhancing healing of chronic VLUs.

The Combination of Platelet Rich Plasma with Skin Needling or Subcision for Postacne Scaring: a Serial Case Study

Scaring in acne remains a common problem and became therapeutic challenges for the clinician. Several modalities were introduced, and combination therapies are required to achieve satisfactory results. Skin needling and subcision reported as effective methods for treating acne scar. The skin needling technique involves puncturing the skin multiple times with a small needle to induce collagen growth, while subcision worked by tunneling the connective tissue and scattered the fibrosis scar. Platelet-rich plasma (PRP) influences wound-healing by stimulating an intense inflammatory response and growth factors, these increase in the production of extracellular matrix and granulation tissue occurs, with vascular in-growth, fibroblastic proliferation, and collagen production also accelerated. Three male patients enrolled in the study, with grades 2-3 acne scar. There are three different methods of treatment were used to see the effect of the treatment. The first patient used the combination of topical PRP and skin needling, and the second patient received a combination of PRP injection and subcision, while the third patient obtains combination between topical PRP and skin needling continued with PRP injection and subcision. Our study revealed that PRP and skin needling or subcision is a simple technique and has the potential for improvement of acne scars. Three weeks after treatment, all three patient had smoother facial skin and reduction of acne scar severity. The combination of PRP and skin needling or and subcision is a simple, safe and rapid method for acne scars treatment.

Efficacy of topical platelet-rich plasma and chitosan co-administration on Candida albicans-infected partial thickness burn wound healing

This study aims to evaluate the effects of topical sole and co-administration of Platelet-rich plasma (PRP) and chitosan on the infected burn wounds model by Candida albicans in Wistar rats. A circle partial thickness contact burn wound model occurred via brass a size of 8mm diameter between the shoulders and after that, 50μl of a suspension containing C. albicans yeast (5×107) colony forming unit was poured on the surface of the wound. The experimental groups included Control, Clotrimazole, PRP and Chitosan+PRP that received any agent, Clotrimazole®, PRP and Chitosan+PRP to treat the wound, respectively. To assess the healing rate, wound size rate, tissue yeast count (TYC) and immunohistochemical staining for heat shock protein 70 (HSP70), HSP 90 and proliferating cell nuclear antigen (PCNA) were conducted. Moreover, enzymatic activity of glutathione peroxidase (GPx), superoxide dismutase (SOD) and malondialdehyde (MDA) rate were evaluated. The results demonstrated that the co-administration of Chitosan+PRP drastically (P<0.05) decreased the wound size and TYC rate comparing to other groups (P<0.05). Immunohistochemical results for HSP 70, HSP 90 and PCNA showed positive reactions and increased intensity in all the treated groups comparing to the control group. Activities of GPx and SOD were higher and the level of MDA was lower all treated groups compared to the control group. A topical use of PRP and chitosan can be advantageous in the infected burn wounds healing.