Tissues Of Albino Rats Research Articles

A study of the antigenic properties of regenerating muscle tissue

The precipitation in gel test after Ouchterlony was used to study the antigenic properties of regenerating muscle tissue in albino rats. It was found that antisera against muscle tissue proteins in the rat form with regenerate extracts a large number of precipitation bands on the 7th, 10th, 14th, 21st and 30th day after the operation than with normal muscle extracts. In addition, it is shown that extracts from regenerates obtained at earlier times after the operation forms more precipitation bands than in the case of later regenerate extracts.

The effect of ACTH on the rate of the glucokinase reaction in albino rat tissues under conditions of acute oxygen lack in the organism

Acute hypoxia induced by the administration of sodium nitrite was found to result in the reduction of glucokinase activity in the tissue. Single ACTH administration in a dose of 2 units depressed the glucokinase activity in the muscles, without affecting the activity of the enzyme in the brain and the testes. Preliminary single ACTH administration prevented the inhibition of glucokinase activity in the brain as observed in hypoxia. Repeated preliminary ACTH injections (2 units daily for 6 days) did not affect the rate of glucokinase reaction in the muscles and the brain, and reduced the glucokinase activity in the testes; the injections also normalized the muscle glucokinase activity inhibited in conditions of hypoxia.

The effect of sarcolysin on the 24-hour periodicity of mitoses in some albino rat tissues

A study was made of the effect produced by sarcolysin (12 mg/kg) on the mitotic activity of various tissues in albino rats following its administration in the morning (6 a. m.) and in the evening (10p. m.) hours. No significant changes of the average 24-h mitotic activity or any disturbance of the 24-h mitoric periodicity were revealed in the corneal epithelium, fascicular and reticular zones of adrenal cortex and in the medulla; this had no relation to the timing of the drug administration.

The effect of toad skin gland secretion on the permeability of tissues to vital stains after their exposure to experimental agute radiation

A study was made of the sorption properties of various albino rat tissues with respect to neutral red at various time periods after gamma irradiation in doses of 400 and 800 r.

Radioactive phosphorus (P32) studies in transplanted carcinoma. I. Incorporation of P32 measured by surface activity in Walker carcinoma and normal tissues of albino rats.

Radioactive phosphorus (P32) studies in transplanted carcinoma. I. Incorporation of P32 measured by surface activity in Walker carcinoma and normal tissues of albino rats.

Histochemical demonstration of disulfide groups in the neurohypophysis under normal and experimental conditions.

SPECIFIC histochemical methods for demonstrating protein-bound sulfhydryl (Barrnett and Seligman, 1952; Barrnett, 1953a) and disulfide groups (Barrnett and Seligman, 1954a) have been recently developed. The distribution of sulfhydryls (Barrnett, 1953a) and disulfldes (Barrnett and Seligman, 1954a) was described in the tissues of albino rats and other animals. In the latter paper the possibility was raised that the sites of localization of disulfide containing protein hormones might be demonstrated in tissue sections if four criteria were satisfied. These would be: (1) that the hormone or its precursor contained disulfides; (2) that disulfides could be demonstrated by a specific method in tissues known to contain the hormone; (3) that available extracts of the hormone could be shown to be rendered insoluble by the same fixation as was applied to tissues and to stain by the same method for disulfides in vitro; (4) that physiological experiments would reveal alteration in the staining reaction in a predictab...

Influence of Streptomycin and Promin on Proliferation of Tubercle Bacilli in the Tissues of Albino Rat

Summary and ConclusionsRats inoculated with a human strain of tubercle bacilli were treated with streptomycin and promin, individually and in combination, and the effect of treatment determined by (a) direct tissue smears, (b) subculturing of lung suspensions, (c) subinoculation of lung suspensions in guinea pigs. Treatment with promin alone showed no beneficial effects, treatment with streptomycin alone resulted in an average lower colony count than in the controls, while treatment with both drugs appeared to indicate sterilization of 41.6% of the animals and marked decrease and attenuation of persisting viable tubercle bacilli in the remainder.

Estimation of Histamine in the Blood and Other Tissues of Rats and Guinea Pigs Infected with Trichinella spiralis

Although the life cycle of Trichinella spiralis (Owen) is well understood and the histopathology and immunology of trichinosis have been extensively investigated, little is known concerning the toxicology of the infection. Since one of the products of tissue destruction is believed to be histamine, and the acute symptoms of trichinosis appear at the height of tissue invasion and presumably tissue destruction, an investigation has been made concerning changes in the histamine content of the blood and certain other tissues of albino rats and guinea pigs experimentally infected with T. spiralis. Several investigators have suggested the possibility that the destruction of tissue and irritation caused by the migrating larvae of T. spiralis might result in the production of toxic materials and chemical changes in the blood. The first attempt to detect such poisons and determine their chemical nature was made by Flury (1913), who reproduced the symptoms of trichinosis by the subcutaneous injections of aqueous and alcoholic extracts of heavily infected muscles. He attributed the effects to no one particular substance but rather to a group of purine bases and creatine derivatives, such as methyl guanidine. Harwood, Spindler, Cross and Cutler (1937) using the method of Minot and Dodd (1933), reported an increase in guanidine in rabbits infected experimentally with T. spiralis. While increased guanidine has been reported in several diseases, its significance has been questioned (Sollman, 1942). Histamine has a more pronounced pharmacological action than does guanidine and may possibly be a significant toxic agent in trichinosis. Increases of this substance in the blood and other tissues have been reported in a number of conditions including severe burns (Rosenthal, 1937), anaphylactic shock (Code, 1939), traumatic shock (Dale and Laidlaw, 1919), and adrenalectomy (Rose and Browne, 1941).

The Mode of Action of Methylcholanthrene on Cultures of Normal Tissues

The mode of action of carcinogenic agents is one of the most fundamental problems of cancer research. Available evidence clearly indicates that the potentiality of malignant transformation of any normal cell type is determined, in part at least, by hereditary factors. It would seem reasonable, therefore, for the study of the mode of action of carcinogens, to select cell types possessing a favorable hereditary constitution and to subject these cells to the influence of suitable carcinogenic agents under controlled conditions. The present report deals primarily with a systematic investigation of the action of methylcholanthrene on cultures of subcutaneous tissue of albino rats and on cultures derived from the ventral musculature of mice of the C3H strain. Methylcholanthrene was chosen because it is one of the most potent chemical carcinogenic agents known and can be secured in a state of the highest purity. A single experiment was done with 1:2:5:6-dibenzanthracene. Work which will be discussed in a subsequent paper has demonstrated that the subcutaneous injection of a lard solution of methylcholanthrene or of dibenzanthracene gives rise to sarcomas at the site of injection in practically all rats of the strains used for our tissue cultures. In a comparative study of eight inbred strains of mice, Andervont (1) found that C3H mice have the highest susceptibility to the development of subcutaneous sarcomas following the injection of these hydrocarbons subcutaneously. Assuming, therefore, that these sarcoma cells are derived from cells of the fibroblast type, it appears that the cultures chosen for this study have an hereditary constitution favorable for carcinogenesis.

The composition of the tissues of albino rats treated with alkaline anterior pituitary extracts

Research Article| January 01 1932 The composition of the tissues of albino rats treated with alkaline anterior pituitary extracts Einar Bierring; Einar Bierring 1The Biochemical Institute of the University of Copenhagen Search for other works by this author on: This Site PubMed Google Scholar Ejnar Nielsen Ejnar Nielsen 1The Biochemical Institute of the University of Copenhagen Search for other works by this author on: This Site PubMed Google Scholar Biochem J (1932) 26 (4): 1015–1021. https://doi.org/10.1042/bj0261015 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Cite Icon Cite Get Permissions Citation Einar Bierring, Ejnar Nielsen; The composition of the tissues of albino rats treated with alkaline anterior pituitary extracts. Biochem J 1 January 1932; 26 (4): 1015–1021. doi: https://doi.org/10.1042/bj0261015 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Journal Search Advanced Search This content is only available as a PDF. © 1932 LONDON: CAMBRIDGE UNIVERSITY PRESS1932 Article PDF first page preview Close Modal You do not currently have access to this content.