Intrinsic neural timescales characterize the dynamics of endogenous fluctuations in neural activity. We measured the intrinsic timescales of prefrontal neurons and examined their changes during posterior parietal cortex (PPC) inactivation. Frontal eye field (FEF) neurons within the prefrontal cortex (PFC) exhibited a bimodal distribution of timescales: short-timescale neurons showed stronger transient visual responses, while long-timescale neurons exhibited stronger sustained modulation during stimulus-driven attention. PPC inactivation increased intrinsic timescales in both neuron types, with a 15-fold greater increase in short-timescale neurons. Additionally, it reduced visual and attentional modulation, particularly impairing attentional modulation in long-timescale neurons. Our results provide the first causal evidence that the intrinsic timescales of FEF neurons depend on PPC and suggest the presence of at least two network motifs with distinct timescales that contribute to neuronal dynamics and functional computations within FEF. The heterogeneity in these timescales may facilitate cognitive flexibility, allowing PFC to adapt to varying task demands.
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