Thiol-disulphide Homeostasis In Patients Research Articles

Thiol-disulphide Homeostasis in Patients with Schizophrenia: The Potential Biomarkers of Oxidative Stress in Acute Exacerbation of Schizophrenia.

: Recent evidence suggests that oxidative stress contributes to the pathophysiology of schizophrenia. This study aimed to compare thiol-disulphide homeostasis in acute and stable phases of schizophrenia for the first time. : Among the patients with schizophrenia, 61 in the acute-phase and 61 in the stable phase of their illness were enrolled in the study. Native thiol (NT), total thiol (TT), disulphide (SS), disulphide/native thiol, disulphide/total thiol, and native thiol/total thiol for thiol-disulphide homeostasis were compared between the groups. The Brief Psychiatric Rating Scale (BPRS), Scale for the Assessment of Positive/Negative Symptoms (SAPS/SANS), Clinical Global Impression-Severity Scale (CGI-S), Barnes Akathisia Rating Scale, and Simpson-Angus Scale were used to assess symptoms. : After controlling for age, sex, body mass index, and smoking status there were significant differences in NT, TT, SS/NT, SS/TT, and NT/TT, but not SS. Thiol/disulphide homeostasis has shifted in favour of the oxidative side in patients with acute-phase compared to that in stable schizophrenia. BPRS, SAPS, and CGI-S scores were significantly correlated with all six thiol-disulphide parameters, but not SANS, when controlling for age and sex. Significant receiver operating characteristic (ROC) curves were obtained for all thiol-disulphide homeostasis parameters. Discriminant analysis was found to be statistically significant in discriminating between groups. : These results show that oxidative status increases thiol-disulphide homeostasis in patients with acute-phase schizophrenia compared to those with stable schizophrenia. These findings suggest that thiol-disulphide parameters can be used as biomarkers for the acute exacerbation of schizophrenia.

Dynamic thiol disulphide homeostasis in patients with surfer's eye: a case-control study.

The role of oxidative stress in the pathogenesis of pterygium is still unclear. However, abnormal thiol disulfide homeostasis levels are involved in the pathogenesis of various systemic or ocular diseases. We aim to analyze dynamic thiol disulphide homeostasis in patients suffering from conjunctival pterygium using a contemporary technique. Thirty-eight subjects suffering from pterygium and 35 age-gender matched healthy volunteers were recruited for the study. For each case, total thiol, disulfide and native thiol levels in blood were obtained. Additionally, the ratio of disulfide over total thiol, native thiol over total thiol and disulfide over native thiol were computed. The level of median native thiol was lower in pterygium group (318.2µmol/L vs. 333.4µmol/L) and median disulfide was slightly higher in pterygium group (24.3µmol/L vs. 22.8µmol/L) compared to control group. Both disulfide over total thiol and disulfide over native thiol ratios were higher in pterygium group, ratio of native thiol over total thiol was found to be higher in control group. Nevertheless, none of those differences were statistically significant at 95% confidence level. Notably, correlation test pointed to a negative correlation both between pterygium grade and native thiol and between total thiol and pterygium grade in pterygium group (P = 0.03 and 0.02, respectively). A negative correlation hinting that slightly weakened dynamic thiol disulphide homeostasis in subjects with pterygium, a local ocular disease. Further studies with larger sample sizes may shed light on this potential relationship and justify systemic antioxidant therapies in these cases.

Comparison of serum dynamic thiol/disulphide homeostasis and nitric oxide levels of total intravenous vs inhaled anaesthesia in endoscopic transsphenoidal pituitary surgery.

Transsphenoidal pituitary surgery (TPS) is traditionally performed under general anaesthesia. This study aimed to compare the effects of total intravenous anaesthesia (TIVA) or sevoflurane, an inhalation anaesthetic, on thiol-disulphide homeostasis in patients undergoing endoscopic endonasal TPS. In this study, 84 patients scheduled for TPS were randomly categorised into two groups: propofol (n=42, the TIVA group) or sevoflurane (n=42, the SEVO group). Blood samples were taken before induction of general anaesthesia and at the 30minutes of postoperation. Serum native thiol and total thiol levels were detected, and the number of dynamic disulphide bonds and related ratios were calculated from these values. Serum nitric oxide (NO) levels were measured using a chemiluminescence method. Although native thiol levels in TIVA postoperation group were markedly increased (P<.05), total thiol levels in SEVO postoperation group were significantly decreased (P<.01). Disulphide levels were declined in both groups (P<.05 for TIVA and P=.001 for SEVO groups). Disulphide/native thiol (P<.05 for both groups) and disulphide/total thiol ratios (P<.05 for TIVA and P<.01 for SEVO groups) were depressed in postoperation groups. We found a marked elevation in native thiol/total thiol ratio in both groups (P<.05 for TIVA and P<.01 for SEVO groups). There was significant augmentation in serum NO levels in the SEVO postoperation group (P<.05). These results are the first to show that both TIVA and sevoflurane showed similar antioxidant effect with reduced disulphide levels, but sevoflurane may offer more robust oxidative stress protection and augmented NO production than TIVA during TPS. However, the clinical effect is needed to further investigate.

Dynamic Thiol Disulphide Homeostasis In Patients Diagnosed With Acute Pulmonary Thromboembolism In Emergency Department

Dynamic Thiol Disulphide Homeostasis In Patients Diagnosed With Acute Pulmonary Thromboembolism In Emergency Department

Thiol-disulphide homeostasis in patients with surgical site infections

Objectives: Introduction: In infectious diseases, various inflammatory cells are active and reactive oxygen species are produced to fight against intra cellular and extra cellular microorganisms. This leads to an increase in potential antioxidant capacity and free radical production. Thiol is an important antioxidant. Thiols enter oxidation reactions with oxidative molecules and it form disulphide bonds. The aim of this study was to evaluate the dynamic thiol/disulphide homeostasis in patients with surgical site infection (SSI). Methods: Sixty-eight patients with SSI and 66 healthy persons (control group) were included in this study. Complete blood count, biochemistry, erythrocyte sedimentation rate, C-reactive protein values and thiol levels were studied in blood of the patients at 0th day and 10th day. The blood thiol disulphide homeostasis was analysed using a new automated method developed by Erel and Neselioglu. Results: Native thiol, total thiol, albumin levels and native thiol/total thiol ratio were found significantly lower in the blood samples at day 0th compared to 10th day. There was a statistically significant difference between the patient group and the control group in IMA, native thiol, total thiol, albumin, disulphide levels, disulphide/native thiol, disulphide/total thiol and native thiol/total thiol ratios Conclusions: The thiol-disulphide balance is impaired in SSI. The elevated disulphide/total thiol ratio and disulphide/native thiol ratio, and reduced native thiol/total thiol ratio indicate increased oxidation in SSI. There is also a strong association between CRP, ESH and thiol-disulphide parameters. Thiol-disulphide homeostasis may potentially be of benefit in inflammatory response. J Microbiol Infect Dis 2020; 10(3):160-166.

Evaluation of thiol-disulphide homeostasis in patients with chronic hepatitis B

Aim: To evaluate the relationship between dynamic thiol-disulfide homeostasis which is a novel oxidative stress marker and levels of HBV DNA, and the oxidant-antioxidant balance. Material and Method: In the controlled study which included chronic hepatitis B (CHB) patients and healthy volunteers, dynamic Thiol-disulphide homeostasis (TDH) was measured using a novel automated method developed by Erel. Disulfide / total thiol (%), disulfide / native thiol (%), and native thiol / total thiol (%) rates were calculated using the previously determined concentrations of disulfides, native thiols, and total thiols. Results: Of thiol / disulfide homeostasis parameters, native thiol, total thiol, and disulfide levels were statistically lower in the CHB patient group (p <0.05). As a result of the correlation analyses, a significant negative correlation was determined between HBV DNA levels and disulfide / native thiol, disulfide / total thiol, and native thiol / total thiol parameters (p <0.05). Conclusions: Our results suggest that oxidative stress increases with the rise in HBV-DNA levels and that the antioxidant defense may have weakened.

Evaluation of oxidative stress in patients with recalcitrant warts.

Warts are benign conditions of the skin and mucosa caused by human papilloma viruses (HPV) that affect many people worldwide. The aim of this study was to evaluate OS by TOS/TAS, levels of 8-hydroxy-2-deoxyguanosine (8-OHdG) an indicator of DNA damage, and also protein oxidation levels by determining the dynamic serum thiol/disulphide homeostasis in patients with warts. We also aimed to investigate whether there is a relationship between thiol/disulphide homeostasis, recalcitrance of warts and DNA damage. Forty patients of age ≥18years, having recalcitrant genital and/or non-genital warts that persisted for more than 2years, 40 patients with warts that persisted for <2years and 40 healthy controls were enrolled in the study. Blood TAS, TOS, OSI, 8-OHdG and dynamic thiol/disulphide homeostasis were evaluated. Significant differences were detected between the groups in the levels of 8-OHdG, TOS, OSI, total thiol, native thiol, reduced thiol, as well as native thiol/total thiol ratio, disulphide/total thiol ratio and disulphide/native thiol ratio. Compared with the controls, patients with recalcitrant warts had significantly higher levels of 8-OHdG, TOS and OSI levels. Total thiol and native thiol levels were significantly lower in patients with recalcitrant warts compared with patients with warts that persisted for <2years. Disulphide levels were significantly higher in the latter group of patients compared with patients with recalcitrant warts and controls. Native thiol/total thiol ratio was significantly higher in both patient groups compared with controls whereas disulphide/total thiol and disulphide/native thiol ratios were significantly lower in both patient groups than in controls. Our findings suggest that impairment of thiol disulphide homeostasis in patients with recalcitrant warts may lead to increased OS and DNA damage. Thus, antioxidant administration with thiol containing proteins may help in the regression of warts and thereby prevent carcinogenesis.

The thiol-disulphide homeostasis in patients with acute pancreatitis and its relation with other blood parameters.

Acute pancreatitis is a common disease seen in emergency departments because of abdominal pain. The present study aims to evaluate the relation between measurements of thiol-disulfide parameters in patients diagnosed with acute pancreatitis and other blood parameters. A total of 56 (56%) patients, who were admitted to the emergency department, and 44 (44%) healthy volunteers participated in this study. A total of 100 samples were taken from the participants. Detailed blood samples were taken from the patients at the time of arrival at the hospital. The thiol-disulfide level in serum was examined using a brand new method that was developed by Erel and Neşelioğlu in the venous blood samples of the patients who were diagnosed with acute pancreatitis during the admission. The data were evaluated in the computer medium. Gallstones were defined as the etiology of AP in 41 patients (73.2%); in one patient, hypertriglyceridemia (1.7%); in four patients, alcohol use (7.1%), and idiopathic 10 patients (17.8%). While the blood thiol levels were low, the disulfide levels were high at a significant level. No statistically significant relations were detected between the amylase, lipase, neutrophil lymphocyte ratio (NLR), which are other blood parameters, and thiol-disulfide balance parameters. The disruption of the thiol-disulfide balance may play a role in the pathogenesis of acute pancreatitis. In acute pancreatitis, since the thiol level is decreased in the blood, administration of the complementary therapies for this thiol deficiency may contribute to the treatment of the disease.

Thiol-disulphide homeostasis in patients with general anxiety disorder and panic disorder

Objective: The aim of this study was to evaluate the dynamic thiol-disulphide homeostasis as a novel oxidative stress parameter in newly diagnosed and medication-naive general anxiety disorder (GAD) and panic disorder (PD) patients. Method: Medication-naive patients with GAD (n=21) or PD (n=23) and healthy individuals (n=23) were enrolled. Patients were administered a sociodemographic form, Beck Depression Inventory, and Beck Anxiety Inventory. Routine biochemical parameters, native thiol, total thiol, and disulphide parameters were measured in all patients and healthy individuals. Results: The native thiol levels of the GAD and PD groups were found significantly lower than those of the control group (p<0.001 and p=0.03, respectively), whereas the disulphide levels and disulphide/native thiol ratios of both patient groups were significantly higher than that of the control group (p<0.001 and p=0.011, respectively). There was no statistically significant difference between the GAD and PD groups with regard to thiol and disulphide levels. Conclusion: Oxidative stress is considered to be one of the molecular changes underlying the pathogenesis of GAD and PD. This study showed that dynamic thiol-disulphide homeostasis was disturbed due to an increase in oxidants and a decrease in antioxidants in GAD and PD patients, suggesting that antioxidant replacement interventions might be an option in the treatment of these disorders.

The strong relationship between disease severity and thiol-disulphide homeostasis in patients with restless legs syndrome

Amac: Bu calisma, primer huzursuz bacak sendromu (RLS) hastalarinda yeni bir oksidatif stres belirteci olan tiyol-disulfid homeostazisini arastirmak amaciyla yapildi. Gerec ve Yontem: Otuz sekiz hasta ve 43 saglikli birey calismaya alindi. Serum nativ tiyol, total tiyol, disulfit ve disulfid / nativ tiyol oranlari yeni bir otomatik yontem kullanilarak olculmustur. RLS tanisi Uluslararasi Huzursuz Bacak Sendromu Calisma Grubu (IRLSSG) tarafindan onerilen kriterlere gore konuldu ve RLS siddeti IRLSSG Siddet Olcegi kullanilarak degerlendirildi. Bulgular: Serum nativ tiyol ve total tiyol duzeyleri anlamli derecede dusuk (sirasiyla p<0.001; p=0.007), disulfid ve disulfid / nativ tiyol orani anlamli olarak daha yuksekti (sirasiyla p=0.017; p<0.001). RLS kontrol grubu ile karsilastirildi. IRLSSG Onem Olcegi ve tiyol-disulfid homeostasisi parametreleri arasinda anlamli korelasyonlar bulundu (r=-0.56, nativ tiyol icin p<0.001; total tiyol icin r=-0.51, p<0.001; disulfid icin r=0.37, p=0.020); r=0.60, disulfid / nativ tiyol yuzdesi orani icin p<0.001). Sonuclar: Azalmis nativ tiyol, artan disulfid seviyeleri ve artmis disulfid / nativ tiyol orani, tiyol-disulfur dengesinin oksidasyon tarafina kaydigini gostermektedir. IRLSSG Onem Olcegi ve tiyol-disulfid homeostasisi parametreleri arasindaki guclu korelasyonlar, homeostazin primer RLS'nin patogenezinde rol oynayabilecegini gostermektedir.

Evaluation of Erythroid Disturbance and Thiol-Disulphide Homeostasis in Patients with Psoriasis.

This study aims to assess how mean corpuscular volume (MCV), red cell distribution width (RDW), and thiol-disulphide homeostasis are altered in psoriasis patients. This is a cross-sectional review of 76 healthy volunteers and 87 psoriasis patients who were consecutively admitted to the department of dermatology. Psoriasis patients and healthy controls were statistically similar with respect to age, sex, body mass index, blood pressures, and disease duration (p > 0.05 for all). When compared to healthy controls, psoriasis patients had significantly higher MCV, RDW, C-reactive protein (CRP), disulphide, disulphide/native thiol, and disulphide/total thiol (p < 0.001 for all). However, psoriasis patients had significantly lower native thiol and native thiol/total thiol (p = 0.009 and p < 0.001, respectively). When compared to healthy controls, the patients with Psoriasis Area Severity Index (PASI) ≤ 10 and patients with PASI > 10 had significantly higher MCV, disulphide, disulphide/native thiol, and disulphide/total thiol (p < 0.001 for all). The patients with PASI ≤ 10 and patients with PASI > 10 had significantly lower native thiol/native thiol than healthy controls (p < 0.001 for all). The psoriasis patients with PASI > 10 had significantly higher RDW and CRP than healthy controls and patients with PASI ≤ 10 (p < 0.001 for all). Disulphide, disulphide/native thiol, disulphide/total thiol, and native thiol/total thiol correlate significantly with both PASI scores and disease duration. Thiol-disulphide homeostasis is enhanced in psoriasis patients. Ongoing inflammation and increased oxidative stress in psoriasis patients also trigger the formation of prooxidants which are neutralized by antioxidants such as thiols. That is why plasma thiol levels are decreased in psoriasis patients.

Impaired thiol-disulphide homeostasis in patients with axonal polyneuropathy

Objective:The objective of this study is to examine thiol-disulphide homeostasis in patients with polyneuropathy dominated by diabetic or non-diabetic axonal degeneration.Materials-methods:Fifty-four patients diagnosed with polyneuropathy dominated by axonal damage and 41 healthy subjects were included in the study. The patients were grouped into two groups according to whether or not they had diabetes. The native thiol and total thiol concentrations were measured with the newly developed automated method.Results:While there was no significant difference between the patients with diabetic and non-diabetic polyneuropathy in terms of native thiol and total thiol levels (p > 0.05), the native thiol and total thiol levels of the groups with both diabetic polyneuropathy and non-diabetic polyneuropathy were significantly low compared to the control group (p < 0.01). The level of disulphides in the patients with diabetic polyneuropathy was significantly higher than that of the patients with non-diabetic polyneuropathy and the healthy individuals (p < 0.05). The loss in the sural nerve sensory neural action potential amplitude was positively correlated with the decrease in the levels of both native thiol and total thiol (p < 0.05).Discussion:In our study, we observed that the thiol-disulphide balance was also impaired in patients with non-diabetic polyneuropathy similar to patients with diabetic polyneuropathy, and we therefore considered that impaired the thiol-disulphide homeostasis could be the last common path in patients with polyneuropathy with axonal damage, regardless of the aetiology. Therefore, fortification of thiol deficiency with N-acetyl cysteine or alpha-lipoic acid can fix the thiol-disulphide balance and help decelerate the axonal damage.

The relationship between severity of ulcerative colitis and thiol-disulphide homeostasis.

The aim of the study was to determine dynamic thiol-disulphide homeostasis in patients with ulcerative colitis, compare it with those of healthy control and to investigate the relationship between the severity of the disease and homeostasisMETHODS: A total of 78 patients and 58 healthy subjects were included in the study. Serum native thiol, total thiol and disulphide amounts were measured by using a novel automated method. Obtained results were compared and relationships were determined by correlation analysis. Serum native thiol, total thiol, disulphide amounts and disulphide/native thiol percent ratio (index) were significantly lower (p = 0.003 for index ratio and p < 0.001 for other parameters) in patients with ulcerative colitis than in healthy controls. Native thiol, total thiol and disulphide amounts were significantly higher (p < 0.001) in patients with ulcerative colitis in remission than in patients with active ulcerative colitis and near to those of healthy control. There were significant negative correlations between the severity of the disease and thiol-disulphide homeostasis parameters (r = -0.55, p < 0.001 for native thiol; r = -0.64, p < 0.0001 for total thiol; r = -0.65, p < 0.001 for disulphide and r = -0.33, p = 0.011 for index). The thiol-disulphide homeostasis was weakened in ulcerative colitis. Strong correlations between the activity of the disease and thiol-disulfide homeostasis indicate that homeostasis may play a role in the pathogenesis of the disease (Tab. 3, Fig. 3, Ref. 30).

Evaluation of oxidative stres by means of thiol-disulphide homeostasis in patients with euthyroid Hashimoto's thyroiditis

Evaluation of oxidative stres by means of thiol-disulphide homeostasis in patients with euthyroid Hashimoto's thyroiditis

Dynamic thiol/disulphide homeostasis in patients with basal cell carcinoma

Background: The aim of this study is to measure and compare the dynamic thiol/disulphide homeostasis of patients with basal cell carcinoma and healthy subjects with a newly developed and original method.Objective: Thirty four patients attending our outpatient clinic and clinically and histopathologically diagnosed as nodular basal cell carcinoma, and age and gender matched 30 healthy individuals have been involved in the study. Thiol/disulphide homeostasis tests have been measured with a novel automatic spectrophotometric method developed and the results have been compared statistically.Results: Serum native thiol and disulphide levels in the patient and control group show a considerable variance statistically (p = 0.028, 0.039, respectively). Total thiol levels do not reveal a considerable variation (p = 0.094). Disulphide/native thiol ratios and native thiol/total thiol ratios also show a considerable variance statistically (p = 0.012, 0.013, 0.010, respectively).Conclusions: Thiol disulphide homeostasis in patients with basal cell carcinoma alters in the way that disulphide gets lower and thiols get higher. Thiol/disulphide level is likely to have a role in basal cell carcinoma pathogenesis.

Evaluation of the level of thiol-disulphide homeostasis in patients with mild and severe preeclampsia.

• We assessed thiol-disulphide balance with a new colorimetric method recently developed by Erel & Neselioglu.

Dynamic Thiol-Disulphide Homeostasis in Patients with Multiple Sclerosis

Objective: The aim of this study was to evaluate thiol-disulphide homeostasis in patients with Multiple Sclerosis (MS) during attack and remission. Design and Methods: A total of 82 patients admitted to our clinic were divided into 2 groups according to whether they are having an attack or not. Peripheral blood samples were taken from all patients and their native thiol, disulphide and total thiol concentrations were measured by a newly developed automated method. In addition, their disulphide/native thiol, disulphide/total thiol and native thiol/total thiol ratios were calculated. Results: The average disulphide values, disulphide/native thiol and disulphide/total thiol ratios of the patients with MS who were having an attack were found to be significantly higher compared to those in remission (p = 0.001, p = 0.001, p = 0.001, respectively). Conclusion: Our results have shown that thiol-disulphide balance was shifted to the oxidative area during the relapse. These homeostasis parameters, which can be easily measured by this newly developed method at low costs, may be important in terms of showing the disease activity and presenting the underlying mechanisms of the disease.

Evaluation of the dynamic thiol-disulphide homeostasis in patients with epilepsy

Evaluation of the dynamic thiol-disulphide homeostasis in patients with epilepsy