Comparison of serum dynamic thiol/disulphide homeostasis and nitric oxide levels of total intravenous vs inhaled anaesthesia in endoscopic transsphenoidal pituitary surgery.

Abstract

Transsphenoidal pituitary surgery (TPS) is traditionally performed under general anaesthesia. This study aimed to compare the effects of total intravenous anaesthesia (TIVA) or sevoflurane, an inhalation anaesthetic, on thiol-disulphide homeostasis in patients undergoing endoscopic endonasal TPS. In this study, 84 patients scheduled for TPS were randomly categorised into two groups: propofol (n=42, the TIVA group) or sevoflurane (n=42, the SEVO group). Blood samples were taken before induction of general anaesthesia and at the 30minutes of postoperation. Serum native thiol and total thiol levels were detected, and the number of dynamic disulphide bonds and related ratios were calculated from these values. Serum nitric oxide (NO) levels were measured using a chemiluminescence method. Although native thiol levels in TIVA postoperation group were markedly increased (P<.05), total thiol levels in SEVO postoperation group were significantly decreased (P<.01). Disulphide levels were declined in both groups (P<.05 for TIVA and P=.001 for SEVO groups). Disulphide/native thiol (P<.05 for both groups) and disulphide/total thiol ratios (P<.05 for TIVA and P<.01 for SEVO groups) were depressed in postoperation groups. We found a marked elevation in native thiol/total thiol ratio in both groups (P<.05 for TIVA and P<.01 for SEVO groups). There was significant augmentation in serum NO levels in the SEVO postoperation group (P<.05). These results are the first to show that both TIVA and sevoflurane showed similar antioxidant effect with reduced disulphide levels, but sevoflurane may offer more robust oxidative stress protection and augmented NO production than TIVA during TPS. However, the clinical effect is needed to further investigate.