This study explores the impact of fixed damper openings on indoor thermal comfort in a lab-scaled chamber. Experiments with maximum, medium, and minimum iris damper openings were conducted under various occupancy conditions. Results show that the maximum opening maintained temperatures close to the ideal 24°C with minimal fluctuations. In contrast, medium and minimum openings resulted in higher temperature variability and less efficient air distribution. Statistical analysis highlighted significant differences in temperature stability among the different damper settings. The maximum opening ensured a stable temperature range, while the minimum opening caused noticeable temperature stratification and discomfort, especially with more occupants. The findings indicate that fixed damper openings are inadequate for maintaining consistent thermal comfort due to their lack of adaptability. This underscores the need for advanced control systems that can dynamically adjust damper positions to ensure optimal indoor temperature regulation and improve energy efficiency.