Through methods such as literature review, on-site investigation, interview, and logical analysis, this study investigates the physical culture, physical system culture, physical behavior culture, and physical moral culture of universities in southwestern Guizhou. The results show that the physical and material culture of the school is the primary foundation for its development. Currently, there are problems in the school, such as insufficient indoor infrastructure, untimely equipment maintenance, and a lack of book resources; In terms of sports system culture, the school's teaching content, extracurricular activities, and competition system are relatively complete, but there is a lack of policy incentives; In terms of sports behavior culture, the school attaches great importance to the development of team cooperation projects, builds a platform for physical exercise, and actively promotes scientific exercise methods. But there are also problems such as insufficient awareness of students' exercise and insufficient cultural penetration of school sports behavior; In terms of sports ethics and culture, schools focus on cultivating awareness of participating in sports activities and guiding healthy sports behavior habits. However, there are also problems such as limited perspectives on students' understanding of problems and the need to improve their ability to resist setbacks. To promote the development of school sports culture, relevant paths are proposed: strengthening infrastructure construction, optimizing management systems, shaping positive sports behavior culture, and cultivating healthy sports moral culture.