Objectives In this study, the influence of bullying experiences and burnout in the workplace of daycare center teachers on turnover intention was examined, and the mediating effect of burnout was analyzed in the relationship between workplace bullying and turnover intention. Methods To this end, a survey was conducted on 451 childcare teachers at daycare centers located across the country. Simple regression analysis and multiple regression analysis were performed on the collected data according to the research questions, the mediating effect was analyzed according to the procedure of Baron and Kenny (1986), and the Sobel Test was conducted for statistical verification. Results First, as a result of analyzing the influence of workplace bullying experience and burnout on the turnover intention of daycare center teachers, it was found that emotional exhaustion and dehumanization among the sub-factors of verbal and non-verbal bullying, work-related bullying, and burnout among the sub-factors of workplace bullying had a significant effect on the teacher's turnover intention. Second, in the relationship between the teacher's workplace bullying experience and turnover intention, burnout was found to have a partial mediating effect, and burnout further increased the turnover intention according to the workplace bullying experience. Conclusions These results suggest that it is necessary to prevent and manage bullying and burnout in the workplace in order to reduce the turnover intention of daycare center teachers, and for this, it is necessary to seek improvement measures in terms of education and institution.