The use of clamp and dowel materials in the arch of the masonry bridges holds pivotal significance in enhancing the seismic resilience of these structures, as these elements contribute crucially to the structural integrity and dynamic response of these venerable structures. For this reason, the seismic performance of the restored historic Plaka Bridge, originally constructed in 1866 in Arta, Greece, and later restored in 2015 after flooding, was investigated considering the dowels and clamps in the arch section of this study. A combination of experimental and numerical analyses was conducted and verified to understand the in-situ interface behavior of the arch stones. In the first phase of the study, the mechanical properties of the dowels and clamps were characterized by the experimental tests. Compression and tension tests were conducted on the stone samples containing dowel bars and clamps respectively to determine the mechanical properties, The experimental outcomes were subsequently validated through Three-Dimensional (3D) Finite Difference Models (FDM). It was obtained that the dowels and clamps represent the bi-linear material model at the interfaces. Finally, the idealized interface behavior was constructed with normal and tangential spring stiffness properties in 3D-FD Models. These springs successfully simulate the interface delamination failure of the stones in all directions. In the final phase of the study, a 3D-FD model of the Plaka Bridge was constructed employing the previously validated normal and tangential stiffness elements in the arch of the bridge. A free-field non-reflecting boundary condition was imposed on the base and lateral boundaries of the bridge model. Seismic analyses were conducted utilizing the significant seismic events including 2023 Kahramanmaraş earthquakes. The results revealed significant alterations in the structural behavior of the bridge under considered earthquakes. Furthermore, the displacements, principal stresses and tensile-compressive damages in the main arch significantly reduced with the adopted stiffness values. This study proposes the undeniably realistic and efficient implementation of interface material modeling for the mechanical connections as opposed to the traditionally assumed mortar properties in literature. Furthermore, the combined behavior of the dowel, clamp, and Khorasan mortar proposes non-linear interface material model to the existing literature by invalidating commonly assumed linear interface behavior in masonry structures.