Modric, T, Clemente, FM, Versic, S, Chmura, P, Andrzejewski, M, Kryściak, J, and Sekulic, D. Environmental heat stress detrimentally affects match running performance of elite soccer teams competing in the UEFA Champions League. J Strength Cond Res XX(X): 000-000, 2024-This study aimed to examine the effect of environmental heat stress (EHS), defined by wet-bulb globe temperature (WBGT), on match running performance (MRP) while controlling for match-related contextual factors. For this purpose, 2 approaches were implemented: (a) the MRP in heat stress nonrisking environment (NoEHS) (WBGT <22° C) and heat stress risking environment (EHSrisk) (WBGT ≥22° C) were compared and (b) changes in MRP according to the 1° C increase in WBGT were evaluated. Data on MRP were collected using an optical tracking system from all teams ( n = 32) in all UEFA Champions League matches ( n = 125) during the 2022-23 season. Metrics included total distance (TD), low-intensity (LIR), moderate-intensity (MIR), and high-intensity running (HIR) distances. Results indicated that less TD (108.84 vs. 110.71 km; Cohen's d [ d ] = 0.53), MIR (15.91 vs. 17.09 km; d = 0.85), and HIR (8.75 vs. 9.23 km; d = 0.46) were covered in matches played in EHSrisk compared with the NoEHS. Further investigation indicated that for every 1° C increase in WBGT, TD averagely decreased by 0.16 km ( d = -0.48), MIR by 0.13 km ( d = -1.09), and HIR by 0.06 km ( d = -0.69). These findings demonstrated decreased overall and intensive workload during the matches in EHSrisk, suggesting the detrimental effect of EHS on match volume and intensity in elite soccer. When EHS risk is anticipated, consideration of playing and recovery strategies should be implemented by coaches to achieve tactical goals while minimizing physical strain during a match.