Synthesis Of Selenium Nanoparticles Research Articles

Phytofabrication of selenium nanoparticles using Azolla pinnata: Evaluation of catalytic properties in oxidation, antioxidant and antimicrobial activities

We demonstrate the green synthesis of selenium nanoparticles with Azolla pinnata plant extract as a reducing and capping agent. The synthesized nanoparticle was characterized through Ultraviolet-Visible analysis, X-Ray Diffraction analysis, Scanning Electron Microscopy-Energy Dispersive X-ray analysis, X-ray Photoelectron Spectroscopy, Fourier Transform Infrared studies. UV-Vis analysis has confirmed the absorption value at 306 nm respectively. The crystallite size of the A. pinnata stabilized nanoparticle was found to be 36.45 nm. The synthesized selenium nanoparticle was spherical in shape. The XPS analysis confirmed that the elements such as selenium a major part with a 3d core level region. The yield percentage was found to be 78.3% from the oxidation of p-Tolualdehyde to p-Toluic acid. The free radical scavenging potential was found to be high at the concentration of 500 μg/mL from the antioxidant study. From the antimicrobial activity, the synthesized Ap-Se NPs showed the maximum zone of inhibition in E. coli 17 ± 0.67 mm followed by E. faecium 15 ± 0.13 mm, C. albicans 15 ± 0.32 mm and S. aureus 14 ± 0.93 mm. Hence, it was proved that the synthesized nanoparticles act as a catalyst in oxidation and effective antioxidant and antimicrobial agents.

Selenium nanoparticles: Synthesis, in-vitro cytotoxicity, antioxidant activity and interaction studies with ct-DNA and HSA, HHb and Cyt c serum proteins

The aim of this study was the synthesis of selenium nanoparticles (SeNPs) employing vitamin C as a biocompatible and low toxic reducing agent. The synthesized selenium nanoparticles were characterized by using UV–vis, FT-IR, SEM-EDX, TEM, DLS, and zeta potential measurements. The results of the DPPH free radical scavenging assay demonstrate that this synthesized nano-selenium has strong potentials to scavenge the free radicals and cytotoxicity against MCF-7 and Raji Burkitt's lymphoma cancer cell lines. The interaction of calf thymus DNA (ct-DNA) with SeNPs indicated that the anticancer activity might be associated with the DNA-binding properties of nano-selenium. Finally, it was found that the synthesized nano-selenium can bind to the most important blood proteins such as human serum albumin (HSA), human hemoglobin (HHb), and Cytochrome c (Cyt c). The results showed that the secondary structure of these proteins remains unchanged, suggesting that the synthesized nano-selenium could be employed as a carrier in the drug delivery system without any cytotoxicity effect.

Selenium nanoparticle synthesis from endangered medicinal herb (Enicostema axillare).

Green synthesis of selenium nanoparticles (SeNPs) was achieved by a simple biological procedure using the reducing power of E. axillare leaf extract. This method is capable of producing SeNPs in a size range of around 56.23-98.18nm, under ambient lab condition. The synthesized nanoparticles were characterized by UV-Vis spectroscopy, Fourier-transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM) and Energy-dispersive X-Ray (EDX). To check the potential hazards of nanotoxicity median lethal concentration (LC50) and Na + /K + -ATPase activity was assessed on zebra fish which showed the LC50 of258.72ppm for 96h and Na + /K + -ATPase seems to significantly decrease with increase in SeNPs concentration. Antibactericidal activity explored the use of SeNPs against wide range of pathogens. Cytotoxicity of SeNPs was assayed against human lung cancer cells (A549) confirmed that SeNPs are able to inhibit the cell growth by dose-dependent manner. In conclusion, green synthesized selenium nanoparticles are less toxic and harmless. This is the first report on green synthesized selenium nanoparticles using E. axillare possessing anti-bacterial and anti-cancer activity.

Structure characterization of Oudemansiella radicata polysaccharide and preparation of selenium nanoparticles to enhance the antioxidant activities

Selenium nanoparticles have attracted worldwide attentions due to their bioactivities and low toxicity, but the application is limited by stability. This study focuses on the synthesis of selenium nanoparticles using polysaccharide as dispersants. Two Oudemansiella radicata polysaccharide fractions (ORP1 and ORP2) were purified by anion exchange and gel filtration chromatography. Monosaccharide composition, methylation analysis, FT-IR and NMR spectra analysis confirmed that the two fractions were mainly composed of →6)-β-D-Glcp-(1→ and →6)-α-D-Galp-(1 → . Selenium nanoparticles (ORP1-SeNPs and ORP2-SeNPs) were prepared by adding ORP1 and ORP2 to the redox system of selenite and ascorbic acid. The synthesis conditions were optimized by single factor experiment and orthogonal experiment. The average diameter of ORP1-SeNPs and ORP2-SeNPs synthesized under the optimal conditions were 106.28 nm and 117.04 nm, and their morphology appear to be monodispersed and spherical. Moreover, stability tests indicated that particle sizes were affected by time and temperature during storage. Compared with selenium nanoparticles without polysaccharide, ORP1-SeNPs and ORP2-SeNPs exhibited higher antioxidant activities by scavenging free radicals (such as DPPH•, ABTS•+ and O2•-).

Bacillus megaterium-Mediated Synthesis of Selenium Nanoparticles and Their Antifungal Activity against Rhizoctonia solani in Faba Bean Plants.

Rhizoctonia root-rot disease causes severe economic losses in a wide range of crops, including Vicia faba worldwide. Currently, biosynthesized nanoparticles have become super-growth promoters as well as antifungal agents. In this study, biosynthesized selenium nanoparticles (Se-NPs) have been examined as growth promoters as well as antifungal agents against Rhizoctonia solani RCMB 031001 in vitro and in vivo. Se-NPs were synthesized biologically by Bacillus megaterium ATCC 55000 and characterized by using UV-Vis spectroscopy, XRD, dynamic light scattering (DLS), and transmission electron microscopy (TEM) imaging. TEM and DLS images showed that Se-NPs are mono-dispersed spheres with a mean diameter of 41.2 nm. Se-NPs improved healthy Vicia faba cv. Giza 716 seed germination, morphological, metabolic indicators, and yield. Furthermore, Se-NPs exhibited influential antifungal activity against R. solani in vitro as well as in vivo. Results revealed that minimum inhibition and minimum fungicidal concentrations of Se-NPs were 0.0625 and 1 mM, respectively. Moreover, Se-NPs were able to decrease the pre-and post-emergence of R. solani damping-off and minimize the severity of root rot disease. The most effective treatment method is found when soaking and spraying were used with each other followed by spraying and then soaking individually. Likewise, Se-NPs improve morphological and metabolic indicators and yield significantly compared with infected control. In conclusion, biosynthesized Se-NPs by B. megaterium ATCC 55000 are a promising and effective agent against R. solani damping-off and root rot diseases in Vicia faba as well as plant growth inducer.

A new method for the selenium nanoparticles synthesis and study of their influence on the characteristics of physiological processes in spring rye seedlings at the initial stages of ontogenesis

A new method for the synthesis of selenium nanoparticles based on its organic compound is described, which makes it possible to obtain nanoparticles with a size of 1.8-3.5 nm. It was established that the growth processes and development of spring rye plants were stimulated by adding the selenium nanoparticles to the growing medium up to 91% and 25% as compared to the control and other selenium-containing preparations, accordingly.

Effect of a nanomodified antibiotic on field strains of E. coli and Enterobacter cloacae

For the first time, a comparative analysis of the biological activity of selenium nanoparticles was carried out to create a new drug based on a non-toxic and effective means of intracellular delivery of selenium nanoparticles and the antibiotic polymyxin. Proven A method for the synthesis of selenium nanoparticles was developed using polyvinylpyrrolidone as an adsorbent, a stable form of their storage was achieved, microscopy of electron losses was carried out, the size of the synthesized preparation containing selenium nanoparticles was determined.

Biogenic selenium nanoparticles (SeNPs) from citrus fruit have anti-bacterial activities

Nanotechnology deals with the synthesis of materials and particles at nanoscale with dimensions of 1–100 nm. Biological synthesis of nanoparticles, using microbes and plants, is the most proficient method in terms of ease of handling and reliability. Core objectives of this study were to synthesize metallic nanoparticles using selenium metal salt from citrus fruit extracts, their characterization and evaluation for antimicrobial activities against pathogenic microbes. In methodology, simple green method was implicated using sodium selenite salt solution and citrus fruit extracts of Grapefruit and Lemon as precursors for synthesizing nanoparticles. Brick red color of the solution indicated towards the synthesis of selenium nanoparticles (SeNPs). Nanoparticle’s initial characterization was done by UV–Vis Spectrophotometry and later FTIR analysis and DLS graphs via Zetasizer were obtained for the confirmation of different physical and chemical parameters of the nanoparticles. Different concentrations of SeNPs were used for antimicrobial testing against E. coli, M. luteus, B. subtilis and K. pneumoniae comparative with the standard antibiotic Ciprofloxacin. SeNPs possessed significant antimicrobial activities against all the bacterial pathogens used. Conclusively, SeNPs made from citrus fruits can act as potent antibacterial candidates.

Green synthesis of selenium nanoparticles mediated from Ceropegia bulbosa Roxb extract and its cytotoxicity, antimicrobial, mosquitocidal and photocatalytic activities

The present study is to design an eco-friendly mode to rapidly synthesize selenium nanoparticles (SeNPs) through Ceropegia bulbosa tuber’s aqueous extracts and confirming SeNPs synthesis by UV–Vis spectroscopy, FT-IR, XRD, FE-SEM-EDS mapping, HR-TEM, DLS and zeta potential analysis. In addition, to assess the anti-cancer efficacy of the SeNPs against the cultured MDA-MB-231, as studies have shown SeNPs biosynthesis downregulates the cancer cells when compared to normal HBL100 cell lines. The study observed the IC50 value of SeNPs against MDA-MB-231 cells was 34 µg/mL for 48 h. Furthermore, the SeNPs promotes growth inhibitory effects of certain clinical pathogens such as Bacillus subtilis and Escherichia coli. Apart, from this the SeNPs has shown larvicidal activity after 24 h exposure in Aedes albopitus mosquito’s larvae with a maximum of 250 g/mL mortality concentration. This is confirmed by the histopathology results taken at the 4th larval stage. The histopathological studies revealed intense deterioration in the hindgut, epithelial cells, mid gut and cortex region of the larvae. Finally, tried to investigate the photocatalytic activity of SeNPs against the toxic dye, methylene blue using halogen lamp and obtained 96% degradation results. Withal computational study SeNPs was shown to exhibit consistent stability towards breast cancer protein BRCA2. Overall, our findings suggest SeNPs as a potent disruptive agent for MDA-MB-231 cells, few pathogens, mosquito larvae and boosts the photocatalytic dye degradation.

Selenium nanostructure: Progress towards green synthesis and functionalization for biomedicine

Selenium is a pivotal micronutrient required for the proper functioning of the body. Deficiency of Se leads to various diseases whereas a high dosage of Se possesses toxicity. To reduce the toxic effects of elemental selenium and to maximize its efficacy, synthesis of selenium at the nanoscale level is important. Selenium nanoparticles (SeNPs) have received considerable attention in the past few decades. Synthesis of nano selenium with unique properties is necessary for its biomedical applications. Synthesis steps involve physical, chemical and biological methods. The biological or green synthesis method is often more advantageous than other available methods because of the major drawbacks involved in those methods. SeNPs synthesised through green route, such as microbes and plants, are summarized in this review. Synthesised SeNPs that is functionalized with different biological moieties are discussed and listed. Green synthesis involves the use of eco-friendly plants and microbes as the route for the synthesis. SeNPs are non-toxic and stable due to the natural capping agent that prevents protein aggregation and maintains the stability of the nanoparticles. To achieve targeted drug delivery of the synthesized nanomaterials and to increase the efficacy of the SeNPs with minimal toxicity, functionalization is an essential tool in the field of nanomedicine. Functionalized SeNPs with various biological moieties offer enhanced cytotoxicity against various cancer cell lines, good stability and targeted drug delivery without non-specific interaction compared to nanoparticle alone. The present review focuses on detailed report and progress towards the green approach for SeNPs synthesis and engineering selenium nanomaterials with different moieties possibly used for various biomedical applications.

Development of Selenium Nanoparticle Based Agriculture Sensor for Heavy Metal Toxicity Detection

The presence of heavy metals in increased concentrations in the environment has become a global environmental concern. This rapid increase in heavy metals in the environment is attributed to enhanced industrial and mining activities. Metal ions possess a lengthy half-life and property to bioaccumulate, are non-biodegradable and, thus, are a threat to the human health. A number of conventional spectroscopic and chromatographic techniques are being used for the detection of heavy metals, but these suffer from various limitations. Nano-based sensors have emerged as potential candidates for the sensitive and selective detection of heavy metals. Thus, the present study was focused on the synthesis of selenium nanoparticles (SeNPs) by using selenite-reducing bacteria in the development of a heavy metal toxicity biosensor. During the biosynthesis of selenium nanoparticles, supernatants of the overnight-grown culture were treated with Na2SeO32− and incubated for 24 h at 37 °C. The as-synthesized nanoparticles were characterized by UV–Vis spectroscopy, X-ray diffraction (XRD), Fourier transform infrared (FTIR) and transmission electron microscopy (TEM) analyses. XRD and TEM results confirmed the formation of SeNPs in sizes ranging from 35 to 40 nm, with face-centered cubic (FCC) structures. The bioreduction process and validation of the formation of SeNPs was further confirmed by FTIR studies. The reduction in the biosynthesis of SeNPs using bacterial metabolite due to heavy metal cytotoxicity was analyzed by the colorimetric bioassay (SE Assay). The inhibition of selenite reduction and loss of red color in the presence of heavy metals may serve as a biosensor for heavy metal toxicity analysis. Thus, this biosensor development is aimed at improving the sensitivity and specificity of analytic detection.

Advances in green synthesis of selenium nanoparticles and their application in food packaging

SummaryThe past decade has seen nanotechnology progressively being adopted by the food industry. Its wide application in food packaging has redefined conservative food packaging with active and intelligent packaging. Nanomaterials do not only influence food quality and safety but also offer health‐related benefits. Selenium nanoparticles (SeNPs) have been preferred in recent years because of their high biological activity. However, they are generally synthesised using physicochemical methods which are associated with toxicity. In the past decade, efforts have been directed towards advancement of green synthesis of SeNPs to minimise hazardous by‐products. The antioxidant and biocidal effects of SeNPs are generally investigated by direct contact between the oxidisable matter and/or test organisms. Lately, there is focus on the effect SeNPs incorporated into packaging films. This paper will review developments on SeNPs synthesised via plant extracts from the year 2010 to present and their potential application in active food packaging.

Speeding up selenite bioremediation using the highly selenite-tolerant strain Providencia rettgeri HF16-A novel mechanism of selenite reduction based on proteomic analysis

Selenite in the environment is extremely biotoxic, thus, the biotransformation of selenite into selenium nanoparticles (SeNPs) by microorganisms is gaining increasing interest. However, the relatively low selenite tolerance and slow processing by known microorganisms limit its application. In this study, a highly selenite-resistant strain (up to 800 mM) was isolated from coalmine soil and identified as Providencia rettgeri HF16. Remarkably, 5 mM selenite was entirely transformed by this strain within 24 h, and SeNPs were detected as early as 2 h of incubation, which is a more rapid conversion than that described for other microorganisms. The SeNPs were spherical in shape with diameters ranging from 120 nm to 295 nm, depending on the incubation time. Moreover, in vitro selenite-reduction activity was detected in the cytoplasmic protein fraction with NADPH or NADH serving as electron donors. Proteomics analysis and key enzyme activity tests revealed the presence of a sulfite reductase-mediated selenite reduction pathway. To our knowledge, this is the first report to identify the involvement of sulfite reductase in selenite reduction under physiological conditions. P. rettgeri HF16 could be a suitable and robust biocatalyst for the bioremediation of selenite, and would accelerate the efficient and economical synthesis of selenium nanoparticles.

Effectiveness of six different methods in green synthesis of selenium nanoparticles using propolis extract: Screening and characterization

AbstractSelenium nanoparticles (Se NPs) were fabricated with propolis hydro-alcoholic extract and six different methods, namely, hydrothermal, microwave irradiation, ultrasonication, UV radiation, self-assembling, and conventional heating. Results indicated that antioxidant activity, turbidity, pH, and brix values of the provided hydroalcoholic propolis extract were 85.8%, 2.235% a.u., 4.1, and 3.2°Bx, respectively. Gas chromatography analysis revealed that approximately 38 bioactive compounds were detected in the provided extract within 40 min of retention time, including chalcone. Results also revealed that each method had advantage in fabrication of Se NPs compared to others, but spherical Se NPs with overall appropriate physicochemical attributes of particle size (50–60 nm), polydispersity index (0.362), zeta potential (−41.8 mV), maximum broad absorption peak (321 nm), and antioxidant activity (12.4%) were synthesized using the ultrasonication method with a frequency of 20 kHz and a power of 300 W for 10 min.

The Biological methods of selenium nanoparticles synthesis, their characteristics and properties

Nanotechnologies have an impact on every sphere of life, change approaches to environmental recovery, introduce new methods of disease analysis and prevention, treatment, drug delivery and gene therapy, affect the provision of environmentally friendly alternative energy sources, increase crop yields, animal and poultry productivity. Physical, chemical, biological methods of synthesis of nanoparticles, selenium in particular, their properties and the factors participating in reduction of metal ions to nanoparticles are considered. Limitations of nanoparticle synthesis inherent in the biological method (identification and isolation of bioactive fragment responsible for biomineralization of metal ions, analysis of ways to develop individual nanoparticles) and factors contributing to the intensification of nanoparticle production (optimization of pH, temperature, contact time, mixing degree) changes in the total charge of functional organic molecules on the cell wall). It has been proved that these factors affect the size, morphology, composition of nanoparticles and their efficiency during the synthesis. The model of green synthesis with the use of physicochemical means and their biomedical applications have been summarized. There are organisms used for the synthesis of NPs - terrestrial and marine bacteria, bacterial extracellular polymeric substances as bioreductants, fungi, yeast, algae, viruses, microorganisms. It has been demonstrated the biochemical ways of microorganisms in order to fight the toxicity of metals during the synthesis of nanoproducts and the factors that determine the toxicity of metals that are converted into nanoparticles (size, shape, coating agent, nanoparticle density and type of pathogen). The biological role of selenium and features of its influence on an organism in a nanoscale scale are shown. Key words: nanotechnologies, nanoselenium, bacteria, green synthesis, enzymes.

A newly isolated Bacillus amyloliquefaciens SRB04 for the synthesis of selenium nanoparticles with potential antibacterial properties.

The aim of this study was to isolate and characterize marine bacterial strains capable of converting selenite to elemental selenium with the formation of Se nanoparticles (SeNPs). For the first time, a novel marine strain belonging to Bacillus amyloliquefaciens (GenBank accession no. MK392020) was isolated from the coast of the Caspian Sea and characterized based on its ability for transformation of selenite to SeNPs under aerobic conditions. The preliminary formation of SeNPs was confirmed via color changes and the products characterized by UV-Vis spectroscopy. The field-emission scanning electron microscopy (FESEM) together with energy-dispersive X-ray (EDX) analysis showed the presence of the spherical SeNPs on both the surface of the bacterial biomass and in the supernatant solution. Dynamic light scattering (DLS) analysis showed the SeNPs to have an average particle size (Z-average) around 45.4-68.3nm. The X-ray diffraction (XRD) studies substantiated the amorphous nature of the biosynthesized SeNPs. Fourier-transform infrared spectroscopic (FTIR) studies of the SeNPs indicated typical proteinaceous and lipid-related bands as capping agents on the SeNPs. Different effective parameters corresponding the yield of SeNPs by B. amyloliquefaciens strain SRB04 were optimized under resting cell strategy. Results showed that the optimal process conditions for SeNP production were 2mM of selenite oxyanion, 20g/L of cell biomass, and 60h reaction time. The synthesized SeNPs had a remarkable antibacterial activity on Staphylococcus aureus compared with chloramphenicol as a broad-spectrum antibiotic.

Green synthesis of Selenium nanoparticles using Capparis decidua and its anti-inflammatory activity

This in vitro study focuses on the green synthesis of selenium nanoparticles using Capparis decidua and its anti-inflammatory activity. Nanotechnology is the speciality associated with Material science and biology, rather than a particular field. It involves the formulation of particles at nanoscale known as Nanoparticles, where they have control over bulk macroscopic properties of the same material. Selenium, being a 'Drug nanocarrier', possesses strong antibacterial, antioxidant and anti-cancer as well as anti-inflammatory properties. As the medicinal plant Capparis decidua possesses a lot of phytochemicals, this study combined it to synthesise selenium nanoparticles, and anti-inflammatory properties were analysed. Synthesis of Selenium nanoparticles using Capparis decidua extract, collection of NPs using centrifugation, analysis of anti-inflammatory using UV spectroscopy and inhibition of Bovine serum albumin denaturation assay were performed. Biosynthesised selenium nanoparticles using Capparis decidua exhibit effective anti-inflammatory properties and act as an alternative candidate for steroidal and non-steroidal anti-inflammatory drugs. On account of performing the Green synthesis of selenium nanoparticles along with the combination of Capparis decidua, it is evident that Capparis decidua possesses effective anti-inflammatory with increasing concentrations up to 5muL. In future, we can encounter further efficacy by raising the concentrations by adding new formulations other than Capparis decidua

Antioxidant activity of Cynodon dactylon mediated selenium nanoparticles

The nanoparticles are known to reduce toxicity, enhance bioactivity and improve targeting cells. The metal and metal oxide nanoparticles such as gold, silver, zinc oxide, zirconium oxide, copper oxide, copper sulfide, selenium, hydroxyl apatite, and titanium oxide were used in several biomedical applications especially diagnosis of cancer, etc. The selenium nanoparticles (SeNPs) are explored due toits unique characteristics and various known therapeutic benefits such as anti bacterial, anti fungal and anti cancer activities. The selenium nanoparticles actively involved in the free radical scavenging activity and anti inflammatory activity. The antioxidant activities and reducing risk of cancer.In this present study, Cynodondactylon or Bermuda grass mediated synthesis of selenium nanoparticles and it was characterized using UV- vis spectrophotometer. And its antioxidant effect was analysed using DPPH assay. The plant extract of Cynodondactylon was prepared using distilled water and mixed with the sodium selenite solution. The solution was kept in a magnetic stirrer at 100 RPM for constant stirring while being observed for any colour change. The results showed that there was a significant antioxidant activity and peak confirm the selenium nanoparticles formation. Finally we conclude that the selenium nanoparticles can be used in various drug designing aspects in future.

A Green Approach to the Bio-based Synthesis of Selenium Nanoparticles from Mining Waste

This study deals with producing selenium nanoparticles (SeNPs) by an economical and reliable process using a mining waste that contains significant amounts of selenium (6.11%), silver (4.12%), lead (4.69%), copper (3.61%), antimony (4.97%) and barium sulfate (70.01%). For separation of valuable metals and obtaining a pure solution for Se synthesis, several steps need to be taken; (a) Barium sulfate removal by gravity separation; (b) Leaching of the concentrate by HNO3 (3M); (c) Precipitation of silver and lead as insoluble chloride salts and (d) removing of other impurities such as arsenic, tellurium and strontium. The solution of purified selenium stock was then utilized as a precursor for the green synthesis of SeNPs using fruit extracts via an eco-friendly approach. The different fruit extracts such as plum, grape, orange, pomegranate, tomatoes, and lime were examined, and orange extract was found to be an efficient, reducing agent as well as a stabilizer for green synthesis of SeNPs. The biosynthesized SeNPs were characterized by various techniques such as SEM, TEM, XRD, FTIR and EDX. The spherical morphology of SeNPs was confirmed by SEM and TEM analysis, and particle size of SeNPs was found between 70 and 80 nm on the basis of the Debye–Scherrer equation.

Preparation and growth-promoting effect of selenium nanoparticles capped by polysaccharide-protein complexes on tilapia.

Compared with traditional inorganic and organic selenium compounds, nano-selenium exhibited higher biological safety and nutritional potency. However, the biological efficacy of nano-selenium has not been comprehensively and accurately evaluated due to its dispersion instability. In this study, novel selenium nanoparticles (SeNPs) with high dispersion stability were successfully prepared using a polysaccharide-protein complex (PSP) as the capping agent. This was isolated from abalone viscera. The average particle size and zeta potential of polysaccharide-protein complex selenium nanoparticles (PSP-SeNPs) were 63.33 nm, and -37.1 mV, respectively. The SeNPs were firmly capped by PSP through SeO and SeN bonds, as demonstrated by X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, and transmission electron microscopy. Due to this capping, the dispersion of PSP-SeNPs remained stable for 12 months at 4 °C, as evidenced by visual inspection and multiple light scattering. Furthermore, PSP-SeNPs imparted an excellent growth-promoting effect on tilapia. The FBW, WGR, and SGR values of tilapia juveniles fed with PSP-SeNPs supplemented diets (0.5-4.5 mg/kg) were significantly higher than those of the control (P < 0.05). A weight gain rate of 4.1%-43.4% and specific growth rate of 0.15%-1.74% were obtained in tilapia during 45-day feeding. The use of marine viscera polysaccharides is a promising, green method for the synthesis of selenium nanoparticles. There are good opportunities for the application of the synthesized PSP-SeNPs in the life sciences. © 2020 Society of Chemical Industry.