On a global scale, cancer is a disease with a tremendous effect on the human population, and has a constant need of new medicines. Scientific and research interest is drawing its attention in the field of biomedical nanotechnology and nanomedicines combining with naturally-derived compounds as they are considered to have less toxic side effects compared to current treatments such as chemotherapy and radiation. Even though plants have a wide range of medical application in cancer, they lack targeted therapeutic efficacy. The synthesis of nanoparticles by green synthesis method overcomes the drawbacks of conventional treatments like chemotherapy, radiation and targeted drug delivery system. The present study is to develop a fast, eco-friendly synthesis of chitosan entrapped Aegle marmelos silver nanoparticle (CEAMAg) using methanolic extract of Aegle marmelos which acts as a reducing agent and has an active principle against cancer activity. The prepared CEAMAg nanoparticles, were characterized using UV visible spectroscopy which showed a peak at 420 nm and 425 nm conforming the reduction of silver and successful chitosan encapsulation. The presence of biomolecules in synthesized CEAMAg Nanoparticles was analyzed using FTIR. The transmission electron microscope (TEM) is the most basic method for measuring the size of nanoparticles and the average particle size distribution of CEAMAg was found to be 15–37 nm. The surface morphology was analyzed by SEM which showed a spherical and hexagonal shapes with 165–358 nm. The particle size and stability was analyzed using DLS and zeta potential which revealed the good conductivity and stability of CEAMAgNp showing 969.4 nm size with 1.74 mV stability. The elemental composition and crystalline structure was analyzed by EDAX and XRD listing the cross linkers elements and structural formation of synthesized Nanoparticle. The cytotoxicity of CEAMAg nanoparticles against cervical cancer was assessed using MTT assay, wound healing assay, DAPI staining and acridine orange and ethidium bromide double staining. The expression pattern of β actin, E−cadherin, N-cadherin, SNAIL, caspase3 and 9 were analyzed using Reverse transcriptase PCR. The results obtained here indicated that the CEAMAg nanoparticles possess dose dependent cytotoxic effect against HeLa cell lines through the inhibition of β actin, N-cadherin, SNAIL and increasing the levels of E-cadherin, caspase3 and 9. In conclusion, synthesized CEAMAgNp exhibited significant anticancer activity against HeLa cell lines via EMT pathway.