CQD are required tool for traceable targeted delivery, biomedical research, and different therapy applications. In this work, we use lemon, papaya and onion which is commonly found in street markets are used for synthesis of carbon dots. Structural and optical properties of synthesized dots were characterized using XRD, Raman, UV – Vis and FTIR spectroscopic methods. Carbon dots suspended in DI water exposed to Mid UV (255 nm – 312 nm) emits fluorescent light of green color confirmed the formation of CDs. UV-Vis characterization showed and absorption prominent peak between 220 to 375 nm. The energy gap of CDs is found to be 3.241 eV. Its refractive index is estimated to be 2.0745. Showing that these CDs are potential candidate for detector applications. XRD spectra depicts that the absorption heap between 2q values 15 to 25 indicate the formation of nanoparticles. It gives an additional information that the powder contains crystalline structure (peaks at 2q values = 24.2, 28.34, 29.8, 30.84, 31.32, 34.08, 40.5, 43.22, 50.16, 58.58, 66.36, 73.66). Raman characterizations indicated the presence of prime functional groups. It is observed that the peaks in the region 1000 – 1250 cm-1 having functional group υ(C=S) and is Raman strong and IR weak. The peak region around 1400-1470 cm-1 δ(CH2) δ(CH3) is in Raman medium and in IR medium. FTIR Characterization the sample is Indicated as two regions finger print and Group and identifying functional Group. In this paper, we explain the current progress of CQD in the biomedical field, focusing on their synthetic methods and characterization with critical insights into facilitating their potential in various applications. Keywords: Carbon Dots, CQD, Green Synthesis Method, Lemon, Onion, Papaya.