ObjectiveTo describe the implementation and outcomes of synchronous video-supported treatment (VOT) as a telemedicine strategy for monitoring tuberculosis treatment in patients from Cali, Colombia. MethodsA quasi-experimental study was conducted in Cali, Colombia. Patients aged ≥ 18 years with newly diagnosed tuberculosis sensitive to first-line drugs were included. The intervention consisted of synchronous video calls to monitor the daily intake of drugs, remotely and in real-time using the WhatsApp mobile application. We described the demographic and clinical characteristics, supervision and treatment adherence process, adverse drug effects, perception of the strategies, and transportation costs. ResultsA total of 23 patients were enrolled in the study for synchronous VOT strategy. The adherence was 99.8% in the intensive phase (2HRZE) and 98.2% in the continuation phase (4HR). All patients completed the tuberculosis treatment, achieving 100% cure criteria for patients with pulmonary tuberculosis. Most adverse effects were reported during the first month of treatment (31.6%). Timesaving was the main advantage reported for the strategy. ConclusionsSynchronous VOT strategy was well tolerated and accepted by all patients, allowing an excellent level of adherence to tuberculosis treatment. Synchronous VOT is proposed as a viable alternative to directly observed therapy for tuberculosis in selected patients.