Yunnan's ethnic herbal medicines possess potent pharmacological effects, offering promising treatments for various ailments while embodying rich cultural heritage. This paper explores the intricate nexus between Yunnan's ethnic medicinal materials, cultural traditions, and human ecology. Through theoretical analysis and comprehensive literature review, it elucidates the transmission and dissemination of herbal knowledge among diverse ethnic groups, highlighting the preferences for specific herbal medicines rooted in climatic, geographical, and cultural influences. The study delves into the profound cultural meanings embedded within herbal medicine practices, revealing their role as symbols of cultural identity, emotional ties, and historical inheritance among ethnic communities. Additionally, an anthropological perspective unveils the complex interplay between culture, ecology, and herbal medicine practices, shedding light on environmental challenges and emphasizing the importance of sustainable living practices rooted in cultural values. While providing valuable insights, this study acknowledges the limitations of relying solely on theoretical analysis and literature review, advocating for interdisciplinary approaches integrating ethnographic fieldwork and biomedical research for a comprehensive understanding of herbal medicine use in Yunnan.