Titanium dioxide (TiO2) is a metal oxide that has been used in textile products for self-cleaning and anti-microbial. Several coating methods were employed by past studies to incorporate TiO2 nanoparticles on cotton fabric. However, fabrics coated with the TiO2 nanoparticles show disadvantages, such as low air and water vapour permeability. Therefore, the current study has introduced the use of cotton yarn. An understanding on the effects of TiO2 coating on cotton yarn has yet to be discovered. Hence, the present study investigated the methods to coat TiO2 on cotton yarn. To achieve this, polysiloxane was used to coat the TiO2 nanoparticles on a cotton yarn. Three coating methods were investigated in the study; A (after), S (simultaneous) and B (before) which are referring to the after, simultaneous and before impregnated and sonication for 1 h at 75 ˚C. From the analysis, the A (after) method gave more desired results compared to the S (simultaneous) and B (before) methods. The A method exhibited the lowest weight loss, which was about 0.30 %, indicating that the TiO2 nanoparticles had a strong adhesion to the cotton surfaces. A large amount of the TiO2 particles was deposited onto the yarn treated with A method, where the estimated weight fraction of titanium element was 14.45 %. The presence of polysiloxane and TiO2 onto the coated yarn samples was confirmed by the FTIR and EDX analyses. The XRD analysis shows that the yarn samples from the A, S, and B methods exhibit similar diffraction peaks at 2θ of 15°,16.5°,22.9°,25.3°, and 28°, indicating the crystal structures of TiO2 and cotton cellulose.